Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,387 C→A 20.1% intergenic (‑166/‑792) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3870C→A20.1% 299.6 ‑0.1 0.0545intergenic (‑166/‑792)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (51/58):  ref base (197/233):  total (251/294)
Fisher's exact test strand distribution p-value = 9.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 581 total aligned reads displayed.

TATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTC  >  NC_014034/1434287‑1434487
                                                                                                        |                                                                                                       
tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTcc                                                                                                         <  2:2542885/101‑1 (MQ=255)
tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTcc                                                                                                         <  2:1016889/101‑1 (MQ=255)
 atatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCg                                                                                                        <  2:4961755/101‑1 (MQ=255)
 atatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCg                                                                                                        >  2:7688733/1‑101 (MQ=255)
 atatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCg                                                                                                        >  1:2103291/1‑101 (MQ=255)
   atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGct                                                                                                      >  1:7940432/1‑101 (MQ=255)
      tgtcGCCTACCGTGGATCGAAACAGGCTGGTTTGGGCGGAACCCCTGGTCCTGCTGGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTg                                                                                                   <  2:604557/98‑1 (MQ=37)
       aCCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGc                                                                                                  >  1:7036227/1‑101 (MQ=255)
        ccGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCt                                                                                                 >  2:343559/1‑101 (MQ=255)
        ccGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCt                                                                                                 <  1:5668642/101‑1 (MQ=255)
          gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTc                                                                                               <  1:2542878/101‑1 (MQ=255)
           cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTcc                                                                                              >  1:5344718/1‑100 (MQ=255)
           cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCa                                                                                              >  2:8246442/1‑101 (MQ=255)
           cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCa                                                                                              <  2:4357176/101‑1 (MQ=255)
               ccGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.Gc                                                                                         <  2:7874280/101‑1 (MQ=255)
                cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCt                                                                                        >  1:4574749/1‑101 (MQ=255)
                cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCt                                                                                        >  1:7597707/1‑101 (MQ=255)
                cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCt                                                                                        >  2:6924855/1‑101 (MQ=255)
                cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCt                                                                                        <  2:6135504/101‑1 (MQ=255)
                  tGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGCGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGc                                                                                      >  1:3902070/1‑101 (MQ=255)
                   ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCg                                                                                     <  1:7870985/101‑1 (MQ=255)
                   ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCg                                                                                     >  2:2283936/1‑101 (MQ=255)
                   ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGCATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCg                                                                                     <  1:6460346/101‑1 (MQ=255)
                     aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTc                                                                                   <  1:4151428/101‑1 (MQ=255)
                      tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTcc                                                                                  >  2:4489226/1‑100 (MQ=255)
                       cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCaa                                                                                 >  2:5164728/1‑101 (MQ=255)
                        gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAAc                                                                                >  1:3391032/1‑101 (MQ=255)
                        gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAAc                                                                                >  1:6302128/1‑101 (MQ=255)
                         aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCAAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAAcc                                                                               >  2:6597241/1‑101 (MQ=255)
                          aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCt                                                                              <  2:1771513/101‑1 (MQ=255)
                           aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                             <  2:9149676/101‑1 (MQ=255)
                            cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..g                                                                          >  2:110938/1‑101 (MQ=255)
                             aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..Gt                                                                         >  1:1845077/1‑101 (MQ=255)
                                 tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCt                                                                     >  1:1148686/1‑101 (MQ=255)
                                  gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTc                                                                    <  2:7577776/101‑1 (MQ=255)
                                    tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTccc                                                                  <  1:8345691/101‑1 (MQ=255)
                                     ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccc                                                                 <  2:8429031/101‑1 (MQ=255)
                                        ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCcg                                                              <  1:5793125/101‑1 (MQ=255)
                                        ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCcg                                                              <  1:6050128/101‑1 (MQ=255)
                                             aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCgggg                                                         <  2:2183316/101‑1 (MQ=255)
                                                 ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                                     <  2:3585177/101‑1 (MQ=255)
                                                 ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                                     <  2:40850/101‑1 (MQ=255)
                                                  cTGATCCTGCTGGGGCGCTCCCCGCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTTTGCTTCACG.GCTGCGTCATCCTT..GTCGCTCCCCCCGCGGGGGCGTg                                                    <  2:3728331/101‑1 (MQ=38)
                                                      tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:5893786/101‑1 (MQ=255)
                                                        ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  1:5524151/94‑1 (MQ=2)
                                                         gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  2:8809126/95‑1 (MQ=2)
                                                         gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGGggcta                                                >  2:1479952/7‑98 (MQ=0)
                                                          ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  2:4874240/96‑1 (MQ=2)
                                                           ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           <  1:4761698/101‑1 (MQ=255)
                                                           ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           <  2:6474057/101‑1 (MQ=255)
                                                           ctgGGTCGATCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGATTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           <  2:5403705/101‑1 (MQ=255)
                                                            atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             >  1:2309858/4‑101 (MQ=2)
                                                            atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             >  1:4495603/4‑101 (MQ=2)
                                                             tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  2:184020/99‑1 (MQ=2)
                                                             gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                         >  2:5639638/1‑101 (MQ=255)
                                                             gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                         <  2:5781825/101‑1 (MQ=255)
                                                             gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                         <  1:2006417/101‑1 (MQ=255)
                                                              cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           <  1:488163/100‑1 (MQ=2)
                                                              cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           >  2:6349878/2‑101 (MQ=2)
                                                              cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GGTTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           <  2:3947337/100‑1 (MQ=2)
                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                       <  2:7057587/101‑1 (MQ=255)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgc                                         <  2:2737897/101‑3 (MQ=2)
                                                                 cGCTCCCCCCGCAGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                        <  1:9117689/101‑4 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                       >  1:4092651/1‑97 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                       >  1:1600412/1‑97 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                    <  1:3306437/101‑1 (MQ=255)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                    >  2:79879/1‑101 (MQ=255)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                      <  1:6906154/101‑6 (MQ=2)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGCTTg                                   >  2:7291494/1‑101 (MQ=255)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                   <  2:7166702/101‑1 (MQ=255)
                                                                    tCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTgt                                  >  2:3157882/1‑100 (MQ=255)
                                                                    tCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                  >  2:3283867/1‑101 (MQ=255)
                                                                     ccccccGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                    >  2:2338575/1‑94 (MQ=2)
                                                                     ccccccGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                    <  1:7427006/101‑8 (MQ=2)
                                                                     ccccccGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                 >  1:6640417/1‑101 (MQ=255)
                                                                     ccccccGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                 >  1:8987469/1‑101 (MQ=255)
                                                                     ccccccGCGGGGGCGTGGATCGAAAC..G..TCTCCCCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                 <  1:6265211/101‑1 (MQ=255)
                                                                      cccccGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                <  2:8590553/101‑1 (MQ=255)
                                                                       ccccGCGGGGGCGTGGATCGAAAC..T..TGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaacgac                                <  1:8791791/101‑8 (MQ=37)
                                                                        cccGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                 <  1:3693557/101‑11 (MQ=0)
                                                                        cacgggggggCGTGGAGCGAAGC..G..TCTCCGCTCTTCTTCCCG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                              <  2:2943857/96‑1 (MQ=21)
                                                                         ccGCGGGGGCGTGGATCGAAAC..G..GCTCCGCTCTGCTTCACG.GCTGCGGCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                             <  2:9000106/101‑1 (MQ=255)
                                                                           gcgGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                           >  2:5883562/1‑101 (MQ=255)
                                                                             gggggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGTGGTGGATCGAAACACGGATTGCATgggtccg                         >  1:7691344/1‑96 (MQ=255)
                                                                             gggggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                         >  1:5147426/1‑101 (MQ=255)
                                                                               gggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCgcg                       >  2:1611077/1‑99 (MQ=255)
                                                                                ggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                      <  1:2734648/101‑1 (MQ=255)
                                                                                  cGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                    <  1:4460719/101‑1 (MQ=255)
                                                                                   gTGGATCGAAAC..G..TCTCCGCCCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGCGAc                   <  1:7204910/101‑1 (MQ=255)
                                                                                     ggATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                 >  2:47265/1‑101 (MQ=255)
                                                                                      gATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAgg                <  1:1413945/101‑1 (MQ=255)
                                                                                       aTCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc               <  2:1326673/101‑1 (MQ=255)
                                                                                         cGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt             >  1:3112023/1‑101 (MQ=255)
                                                                                         cGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt             >  1:107803/1‑101 (MQ=255)
                                                                                          gAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt            <  1:8177462/101‑1 (MQ=255)
                                                                                          gAAAC..G..GCTCCGCTCTTCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt            <  1:8696868/101‑1 (MQ=255)
                                                                                            aaC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc          >  2:5313773/1‑101 (MQ=255)
                                                                                              c..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg        <  1:848887/101‑1 (MQ=255)
                                                                                              c..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACAGATTGCATGGCGCCGAGGGACAGGCTTTGCAg        >  2:6750936/1‑101 (MQ=255)
                                                                                                        cGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc  <  2:1972192/101‑1 (MQ=255)
                                                                                                        |                                                                                                       
TATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTC  >  NC_014034/1434287‑1434487

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG