Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,392 T→C 1.1% intergenic (‑171/‑787) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3920T→C 1.1% 2.8 0.0 ‑0.5538intergenic (‑171/‑787)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (3/3):  ref base (237/295):  total (240/298)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.24e-01

Only 100 of 575 total aligned reads displayed.

GCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCC  >  NC_014034/1434292‑1434492
                                                                                                       |                                                                                                      
 cACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTg                                                                                                       <  2:3570029/101‑1 (MQ=255)
  aCCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGc                                                                                                      >  2:7062490/1‑101 (MQ=255)
   ccGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCt                                                                                                     <  1:5668642/101‑1 (MQ=255)
     gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTc                                                                                                   <  2:6448685/101‑1 (MQ=255)
     gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTc                                                                                                   <  1:2542878/101‑1 (MQ=255)
     gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTCCTTc                                                                                                   >  2:5823208/1‑101 (MQ=255)
     cgatctcGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTc                                                                                                   <  2:5379403/95‑1 (MQ=255)
      cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTcc                                                                                                  >  1:5344718/1‑100 (MQ=255)
      cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCa                                                                                                  <  2:4357176/101‑1 (MQ=255)
              ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.g                                                                                        <  1:7870985/101‑1 (MQ=255)
              ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGCATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.g                                                                                        <  1:6460346/101‑1 (MQ=255)
                aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCCCGGGGGCGTGGAGCGAAAA..GTCTCCGCCCTCCCTCAAG.GCTggcgg                                                                                       >  1:2810309/1‑97 (MQ=37)
                 tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCa                                                                                     <  1:4037232/101‑1 (MQ=255)
                  cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCaa                                                                                    >  2:1041980/1‑101 (MQ=255)
                   gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAAc                                                                                   <  2:7940092/101‑1 (MQ=255)
                     aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCt                                                                                 <  2:1771513/101‑1 (MQ=255)
                      aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCtt                                                                                <  2:9149676/101‑1 (MQ=255)
                         ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTc                                                                             <  1:3383618/101‑1 (MQ=255)
                            tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCt                                                                          <  2:2307424/101‑1 (MQ=255)
                            tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCt                                                                          <  2:4574614/101‑1 (MQ=255)
                             gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGATCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTc                                                                         >  1:7670070/1‑101 (MQ=255)
                                ttGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTGTGCTTCACG.GCTGC.GTCAACCTTGTCGCTcccc                                                                      <  1:8199213/101‑1 (MQ=255)
                                 tGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTccccc                                                                     <  2:6974644/101‑1 (MQ=255)
                                  gggCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTcccccc                                                                    <  1:1425989/101‑1 (MQ=255)
                                  gggCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTcccccc                                                                    >  1:2030782/1‑101 (MQ=255)
                                   ggCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCcg                                                                   <  2:3967990/101‑1 (MQ=255)
                                   ggCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCcg                                                                   <  1:6050128/101‑1 (MQ=255)
                                       gAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCggg                                                               >  1:9083024/1‑101 (MQ=255)
                                        aaCACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCgggg                                                              >  2:8314299/1‑101 (MQ=255)
                                         acacCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCCCggggg                                                             >  1:7946521/1‑101 (MQ=255)
                                          cacCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGc                                                            <  2:7241718/101‑1 (MQ=255)
                                           acCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCg                                                           >  2:3929476/1‑101 (MQ=255)
                                            ccTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGt                                                          <  1:4489361/101‑1 (MQ=255)
                                              tGATCCTGCTGGGTCGCTCCCCCCCCCGGGGGGGGGGATCGAACC..GCCTCCGCTCTGCTT.ACC.GCCGCGGTCACCCTTGTCGCTCCCCCCCCGGGGGCggg                                                         >  2:1141811/1‑99 (MQ=255)
                                              tGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTgg                                                        >  2:4993372/1‑101 (MQ=255)
                                                gcgggctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTg                                                         <  1:8705621/91‑1 (MQ=2)
                                                gcgggctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTg                                                         >  1:4549741/11‑101 (MQ=2)
                                                aTCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGac                                                      >  2:225014/1‑100 (MQ=255)
                                                aTCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      >  2:1912120/1‑101 (MQ=255)
                                                 tCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:5893786/101‑1 (MQ=255)
                                                  ccTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  2:7437336/1‑101 (MQ=255)
                                                  ccTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  2:343357/101‑1 (MQ=255)
                                                  ccTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  1:3796571/101‑1 (MQ=255)
                                                  ccTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  2:4788773/101‑1 (MQ=255)
                                                   ggctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  1:5524151/94‑1 (MQ=2)
                                                   ggctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      >  2:4926267/8‑101 (MQ=2)
                                                   ctgctgGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   >  1:3063875/1‑101 (MQ=255)
                                                   ctgctgGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   >  1:4433366/1‑101 (MQ=255)
                                                    gctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     >  2:3689168/7‑101 (MQ=2)
                                                    gctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  2:8809126/95‑1 (MQ=2)
                                                     gctgGGTCGGT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  2:2914350/101‑1 (MQ=255)
                                                     ctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  2:5668430/6‑101 (MQ=2)
                                                       tgGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGCGGCGGggggcatcacc                                               <  2:7597380/101‑10 (MQ=37)
                                                       atcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  1:7275722/98‑1 (MQ=2)
                                                        tcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  2:8513932/99‑1 (MQ=2)
                                                         ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                             <  2:7847782/101‑1 (MQ=255)
                                                         ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                             <  2:5517771/101‑1 (MQ=255)
                                                         cgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                <  1:488163/100‑1 (MQ=2)
                                                         cgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                >  2:6347948/2‑101 (MQ=2)
                                                         cgTCGCG.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                <  2:6537707/100‑1 (MQ=2)
                                                           tCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                           >  2:6131633/1‑101 (MQ=255)
                                                            cGCT.CCCCCCGCGGGGGCGTGGATCGNAAC..NTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                          <  1:5788804/101‑1 (MQ=255)
                                                            cGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                             >  1:6971326/1‑98 (MQ=2)
                                                            cGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAaccct                                             >  1:1212164/1‑98 (MQ=2)
                                                                 ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                         >  1:8987434/1‑94 (MQ=2)
                                                                 ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                         >  2:2698435/1‑94 (MQ=2)
                                                                 ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                      >  2:2358207/1‑101 (MQ=255)
                                                                   ccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgac                                       <  1:8791923/101‑10 (MQ=2)
                                                                    cccGCGGGGGCGTGGATCGAAAC..GTCGCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                   <  2:5289921/101‑1 (MQ=255)
                                                                     ccGCGGGGGCGTGGATCGAAAC..GGCTCCGCTCTGCTTCACG.GCTGC.GGCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                  <  2:9000106/101‑1 (MQ=255)
                                                                        cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                               >  1:3674258/1‑101 (MQ=255)
                                                                        cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                               >  2:3057753/1‑101 (MQ=255)
                                                                        cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                               <  2:1831073/101‑1 (MQ=255)
                                                                         gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGTGGTGGATCGAAACACGGATTGCATgggtccg                              >  1:7691344/1‑96 (MQ=255)
                                                                         gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                              >  1:5147426/1‑101 (MQ=255)
                                                                           gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                            >  1:7487776/1‑101 (MQ=255)
                                                                           gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                            <  2:682952/101‑1 (MQ=255)
                                                                           gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                            <  1:8528825/101‑1 (MQ=255)
                                                                            ggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTTGCTCCCCCCGCGGGGGCGTCGATCGAAACACGGATTGCATGGCGCCGAgg                           >  2:1599192/1‑101 (MQ=255)
                                                                               gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGGGGGGTTCAAAACCGGGATGGAAGGGCGCGGAgggca                        >  1:1625765/1‑99 (MQ=25)
                                                                               gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                        <  1:8412567/101‑1 (MQ=255)
                                                                                tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                       >  1:414131/1‑101 (MQ=255)
                                                                                 ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                      >  2:6733253/1‑101 (MQ=255)
                                                                                 ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                      >  2:4482443/1‑101 (MQ=255)
                                                                                 ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                      >  1:6695722/1‑101 (MQ=255)
                                                                                  gATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAgg                     >  2:8016641/1‑101 (MQ=255)
                                                                                   aTCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc                    >  1:2245958/1‑101 (MQ=255)
                                                                                   aTCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc                    <  2:1326673/101‑1 (MQ=255)
                                                                                     cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                  <  2:3233014/101‑1 (MQ=255)
                                                                                     cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                  >  1:6277393/1‑101 (MQ=255)
                                                                                     cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                  >  1:1968616/1‑101 (MQ=255)
                                                                                     cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                  >  1:107803/1‑101 (MQ=255)
                                                                                      gAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                 >  2:1476317/1‑101 (MQ=255)
                                                                                         aC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa              <  1:7062775/101‑1 (MQ=255)
                                                                                              tctcCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc           <  1:4806477/101‑1 (MQ=255)
                                                                                               ctcCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg          <  1:6302553/101‑1 (MQ=255)
                                                                                                  cGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc       <  2:8612867/101‑1 (MQ=255)
                                                                                                   gCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc      >  2:2149746/1‑101 (MQ=255)
                                                                                                       tGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc  <  2:6014629/101‑1 (MQ=255)
                                                                                                       tGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc  <  1:3283967/101‑1 (MQ=255)
                                                                                                       |                                                                                                      
GCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCC  >  NC_014034/1434292‑1434492

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG