Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,397 C→G 20.3% intergenic (‑176/‑782) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3970C→G20.3% 286.1 ‑0.4 ‑0.0537intergenic (‑176/‑782)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (50/58):  ref base (185/239):  total (237/300)
Fisher's exact test strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.53e-01

Only 100 of 565 total aligned reads displayed.

GCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGG  >  NC_014034/1434297‑1434496
                                                                                                          |                                                                                                       
 cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCa                                                                                                        >  1:3275012/1‑101 (MQ=255)
     ccGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.Gc                                                                                                   <  2:7874280/101‑1 (MQ=255)
      cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCt                                                                                                  >  2:5397679/1‑101 (MQ=255)
      cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCt                                                                                                  >  2:6924855/1‑101 (MQ=255)
       gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTg                                                                                                 >  1:4846203/1‑101 (MQ=255)
       gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTg                                                                                                 <  2:1400437/101‑1 (MQ=255)
         ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCTCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.Cg                                                                                              >  1:3371110/1‑101 (MQ=255)
             cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCaa                                                                                          >  2:5164728/1‑101 (MQ=255)
              gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                         >  1:6302128/1‑101 (MQ=255)
               aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGATCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAcc                                                                                        >  2:6086804/1‑101 (MQ=255)
                aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCt                                                                                       >  1:7445536/1‑101 (MQ=255)
                 aCAGGCTGCTTTGGGCGGAACCCCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCtt                                                                                      <  2:1628571/101‑1 (MQ=255)
                 aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCtt                                                                                      <  2:7570654/101‑1 (MQ=255)
                 aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCtt                                                                                      <  1:782941/101‑1 (MQ=255)
                    ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTc                                                                                 >  2:5893551/1‑101 (MQ=255)
                        gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTc                                                                             <  2:1500665/101‑1 (MQ=255)
                         ctTTAGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcc                                                                            <  2:7979285/101‑1 (MQ=255)
                          tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccc                                                                           <  1:8345691/101‑1 (MQ=255)
                           ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccc                                                                          <  2:8429031/101‑1 (MQ=255)
                            tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccccc                                                                         <  1:4749334/101‑1 (MQ=255)
                             gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccccc                                                                        <  1:1425989/101‑1 (MQ=255)
                             gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccccc                                                                        >  1:2030782/1‑101 (MQ=255)
                              ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCANCCTT..GTCGCTCCCCCcg                                                                       >  2:2419844/1‑101 (MQ=255)
                                cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCcgcg                                                                     <  1:6269598/101‑1 (MQ=255)
                                 ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCgg                                                                    >  1:8182525/1‑101 (MQ=255)
                                  gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCggg                                                                   >  2:5587558/1‑101 (MQ=255)
                                        cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTg                                                             <  2:3630391/101‑1 (MQ=255)
                                        cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTg                                                             <  1:2134545/101‑1 (MQ=255)
                                           gcgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTg                                                             <  1:8705621/91‑1 (MQ=2)
                                           aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGGgggt                                                          >  2:3253966/1‑99 (MQ=255)
                                            tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  1:5893786/101‑1 (MQ=255)
                                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGCTCg                                                        >  2:3744877/1‑101 (MQ=255)
                                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        <  2:343357/101‑1 (MQ=255)
                                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        <  1:4335561/101‑1 (MQ=255)
                                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        <  2:4130292/101‑1 (MQ=255)
                                              ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                          <  2:6475276/94‑1 (MQ=2)
                                              ggctagcgTCGCTCCCCCCGCGGCGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                          <  2:2935419/94‑1 (MQ=2)
                                              ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                       >  2:9182526/1‑101 (MQ=255)
                                               gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGGTc                                                         >  1:4488511/7‑101 (MQ=2)
                                               gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:2150852/95‑1 (MQ=2)
                                               gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         >  2:6573099/7‑101 (MQ=2)
                                                ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        <  2:4874240/96‑1 (MQ=2)
                                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                   >  1:3536847/1‑101 (MQ=255)
                                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                   >  2:8767354/1‑101 (MQ=255)
                                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                   <  2:177833/101‑1 (MQ=255)
                                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCCCCGGGGCGCGGCTAGAAaca                                                   >  2:6262971/1‑101 (MQ=37)
                                                  atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      <  1:7275722/98‑1 (MQ=2)
                                                   tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     >  1:3034259/3‑101 (MQ=2)
                                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                  <  1:3769151/101‑1 (MQ=255)
                                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                  <  2:5781825/101‑1 (MQ=255)
                                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                  <  1:6925140/101‑1 (MQ=255)
                                                    ggTCGCTCCCCCCGCGGGGGCGCGGATCGAAAC..G..TCTCCT.CTCTG.CTTCACG.GCTG.CGACAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                                 <  1:1606295/101‑1 (MQ=255)
                                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GGTTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                    <  2:3947337/100‑1 (MQ=2)
                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                <  2:1148668/101‑1 (MQ=255)
                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..CTGCTGACACCGCCAACAAA.TATGTGGTCAACACT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                   >  2:184771/1‑100 (MQ=2)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                               >  1:1882574/1‑101 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGNAAC..N..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                              <  1:5788804/101‑1 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGGGGATCGAAaccct                                                 >  1:1212164/1‑98 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                              <  2:201222/101‑1 (MQ=255)
                                                       cGCTCCCCCCGCAGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                 <  1:9117689/101‑4 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                             <  2:8846213/101‑1 (MQ=255)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                               <  1:7919934/101‑6 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGCTTg                                            >  2:7291494/1‑101 (MQ=255)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                            <  2:7166702/101‑1 (MQ=255)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                              >  1:5579428/1‑95 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                              >  1:5266945/1‑95 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                             >  2:2338575/1‑94 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                             <  2:3140834/101‑8 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                             >  1:4967330/1‑94 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                          >  1:8987469/1‑101 (MQ=255)
                                                           ccccccGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                          >  1:6640417/1‑101 (MQ=255)
                                                            cccccGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                            <  2:9042635/101‑9 (MQ=2)
                                                             ccccGCGGGGGCGTGGATCGAAACGGG..TCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgac                                           <  1:8791923/101‑10 (MQ=2)
                                                             ccccGCGGGGGCGTGGATCGAAAC..T..TGTCTG.ACGCG.CTCATCA.GC.A.CGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                         >  2:7049686/1‑94 (MQ=37)
                                                             ccccGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                        <  1:6428934/101‑1 (MQ=255)
                                                             ccccGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                        <  2:8549517/101‑1 (MQ=255)
                                                                     gggCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                <  1:8528825/101‑1 (MQ=255)
                                                                      ggCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                               <  1:2734648/101‑1 (MQ=255)
                                                                          tGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                           <  2:6752850/101‑1 (MQ=255)
                                                                          tGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                           <  1:5397863/101‑1 (MQ=255)
                                                                           ggATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                          <  1:604482/101‑1 (MQ=255)
                                                                               cGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                      >  1:107803/1‑101 (MQ=255)
                                                                                  aaC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                   >  2:5313773/1‑101 (MQ=255)
                                                                                   aC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa                  <  1:7062775/101‑1 (MQ=255)
                                                                                   aC..G..ACTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCATTGcg                  >  2:823431/1‑100 (MQ=255)
                                                                                       g..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                <  2:4445949/101‑1 (MQ=255)
                                                                                          tctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc               <  1:4099684/101‑1 (MQ=255)
                                                                                          tctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc               <  2:22564/101‑1 (MQ=255)
                                                                                           ctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg              >  2:6350214/1‑101 (MQ=255)
                                                                                           ctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg              <  1:4519346/101‑1 (MQ=255)
                                                                                           ctcCG.ATCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg              <  1:2539399/101‑1 (MQ=255)
                                                                                              cG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc           >  2:4956640/1‑101 (MQ=255)
                                                                                               g.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGCCAGGCTTTGCAggacgtcc          >  1:4017369/1‑94 (MQ=255)
                                                                                               g.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc          <  1:7602872/101‑1 (MQ=255)
                                                                                               g.CTCTG.CTTCAAG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc          <  2:765171/101‑1 (MQ=255)
                                                                                                 ctctG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccc         <  1:7689059/101‑1 (MQ=255)
                                                                                                  tctG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc        <  2:4534157/101‑1 (MQ=255)
                                                                                                   ctG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc       <  2:4636338/101‑1 (MQ=255)
                                                                                                         tCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg  >  2:2734303/1‑101 (MQ=255)
                                                                                                         tCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg  <  2:107803/101‑1 (MQ=255)
                                                                                                          |                                                                                                       
GCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGG  >  NC_014034/1434297‑1434496

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG