Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,404 G→A 0.9% intergenic (‑183/‑775) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4040G→A 0.9% 2.5 0.0 ‑0.0521intergenic (‑183/‑775)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (225/291):  total (227/294)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.27e-01

Only 100 of 544 total aligned reads displayed.

GTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1434304‑1434504
                                                                                                         |                                                                                                      
 tGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGCGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGc                                                                                                       >  1:3902070/1‑101 (MQ=255)
         aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAATC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCt                                                                                               <  1:6463448/101‑1 (MQ=255)
          aCAGGCTGCTTTGGGCGGAACCCCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                                              <  2:1628571/101‑1 (MQ=255)
          aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGCCGCTCCCCCCGCGGCGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                                              >  1:9000528/1‑101 (MQ=255)
           cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..g                                                                                           <  1:1976671/101‑1 (MQ=255)
                 gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTc                                                                                     <  2:1500665/101‑1 (MQ=255)
                 gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTc                                                                                     <  2:7577776/101‑1 (MQ=255)
                 gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGATCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTc                                                                                     >  1:7670070/1‑101 (MQ=255)
                  ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcc                                                                                    >  2:4867871/1‑101 (MQ=255)
                  ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcc                                                                                    >  1:3455528/1‑101 (MQ=255)
                    ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccc                                                                                  >  1:6714588/1‑101 (MQ=255)
                       ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCANCCTT..GTCGCTCCCCCcg                                                                               >  2:2419844/1‑101 (MQ=255)
                       ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCcg                                                                               >  2:4052354/1‑101 (MQ=255)
                       ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCcg                                                                               <  2:1896482/101‑1 (MQ=255)
                            aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCgggg                                                                          <  2:2183316/101‑1 (MQ=255)
                              cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGc                                                                        <  1:1370762/101‑1 (MQ=255)
                                ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                                                      <  1:4489361/101‑1 (MQ=255)
                                ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                                                      <  2:526765/101‑1 (MQ=255)
                                 cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTg                                                                     <  2:3630391/101‑1 (MQ=255)
                                 cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTg                                                                     >  2:6222430/1‑101 (MQ=255)
                                  tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  1:7167006/1‑101 (MQ=255)
                                   gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  1:2299263/101‑1 (MQ=255)
                                    gcgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTg                                                                     >  1:4549741/11‑101 (MQ=2)
                                    aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGGgggt                                                                  >  2:3253966/1‑99 (MQ=255)
                                     cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                    <  1:1142919/92‑1 (MQ=2)
                                     cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                    <  2:4508304/92‑1 (MQ=2)
                                     cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                    <  1:5837120/92‑1 (MQ=2)
                                      ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGCTCg                                                                >  2:3744877/1‑101 (MQ=255)
                                       ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:6067518/94‑1 (MQ=2)
                                       ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  1:5524151/94‑1 (MQ=2)
                                       ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               >  2:9182526/1‑101 (MQ=255)
                                       ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               >  1:4433366/1‑101 (MQ=255)
                                        tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  2:5377689/1‑101 (MQ=255)
                                        tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  1:5676098/1‑101 (MQ=255)
                                        gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  2:7885961/95‑1 (MQ=2)
                                        gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 >  1:2038027/7‑101 (MQ=2)
                                         gctgGGTCGGTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  2:2914350/101‑1 (MQ=255)
                                         ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                >  2:5668430/6‑101 (MQ=2)
                                         ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  1:178397/96‑1 (MQ=2)
                                         ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  2:1800390/96‑1 (MQ=2)
                                          ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                            <  1:1592449/101‑1 (MQ=255)
                                           tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                           >  1:3307249/1‑101 (MQ=255)
                                           atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  1:2833716/4‑101 (MQ=2)
                                             ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                                         <  2:5517771/101‑1 (MQ=255)
                                             cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                            >  2:6349878/2‑101 (MQ=2)
                                             cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GGTTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                            <  2:3947337/100‑1 (MQ=2)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                           >  1:5303036/1‑100 (MQ=2)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                        <  2:1148668/101‑1 (MQ=255)
                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCCACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                                       <  2:4427349/101‑1 (MQ=255)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                         >  1:6971326/1‑98 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                                      >  2:460174/1‑101 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                     >  2:79879/1‑101 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                     <  2:8781499/101‑1 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                     <  1:4052469/101‑1 (MQ=255)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGCTTg                                                    >  2:7291494/1‑101 (MQ=255)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                                   >  1:4913538/1‑101 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                     <  2:3140834/101‑8 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                                  >  1:9128754/1‑101 (MQ=255)
                                                      ccccGCGGGGGCGTGGATCGAAAC..T..TGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                 >  2:720983/1‑94 (MQ=37)
                                                      ccccGCGGGGGCGTGGATCGAAAC..T..TGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                 >  2:7049686/1‑94 (MQ=37)
                                                      ccccGCGGGGGCGTGGATCGAAAC..T..TGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                 <  2:888062/101‑8 (MQ=37)
                                                       cccGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                                  <  1:3693557/101‑11 (MQ=0)
                                                       cccGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                               <  1:6087041/101‑1 (MQ=255)
                                                          gcgGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                            >  2:5883562/1‑101 (MQ=255)
                                                           cgGGGGCGTGGATCGAAGC..G..TCTGCGCTCTGCTTCACG.GGTGCGTCCACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                           <  2:1091079/101‑1 (MQ=21)
                                                            gggggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGTGGTGGATCGAAACACGGATTGCATgggtccg                                          >  1:7691344/1‑96 (MQ=255)
                                                            gggggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                          <  2:3455424/101‑1 (MQ=255)
                                                            gggggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                          >  1:8750597/1‑101 (MQ=255)
                                                            gggggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                          >  1:5147426/1‑101 (MQ=255)
                                                              gggGGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                        >  1:8805415/1‑101 (MQ=255)
                                                              gggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                        >  1:7487776/1‑101 (MQ=255)
                                                              gggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                        <  1:8528825/101‑1 (MQ=255)
                                                              gggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                        >  1:7367438/1‑101 (MQ=255)
                                                                  gTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                    <  1:8412567/101‑1 (MQ=255)
                                                                  gTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                    <  2:4965219/101‑1 (MQ=255)
                                                                   tGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                   <  2:2331908/101‑1 (MQ=255)
                                                                   tGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                   <  1:3171976/101‑1 (MQ=255)
                                                                        cGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                              >  1:6277393/1‑101 (MQ=255)
                                                                        cGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                              <  2:9048746/101‑1 (MQ=255)
                                                                         gAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                             <  2:2103229/101‑1 (MQ=255)
                                                                          aaaC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                            <  2:6924661/101‑1 (MQ=255)
                                                                          aaaC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                            <  1:79879/101‑1 (MQ=255)
                                                                           aaC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                           >  1:2610157/1‑101 (MQ=255)
                                                                           aaC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                           >  1:7878248/1‑101 (MQ=255)
                                                                            aC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa                          >  1:4142041/1‑101 (MQ=255)
                                                                            aC..G..ACTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCATTGcg                          >  2:823431/1‑100 (MQ=255)
                                                                             c..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACAGATTGCATGGCGCCGAGGGACAGGCTTTGCAg                         >  2:6750936/1‑101 (MQ=255)
                                                                                g..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtttagatc                        >  2:84865/1‑96 (MQ=255)
                                                                                g..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                        >  1:6008392/1‑101 (MQ=255)
                                                                                   tctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                       <  2:22564/101‑1 (MQ=255)
                                                                                   tctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                       <  1:4806477/101‑1 (MQ=255)
                                                                                   tctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                       >  2:6479084/1‑101 (MQ=255)
                                                                                        gCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                  >  2:142760/1‑101 (MQ=255)
                                                                                        gCTCTGCTTCAAG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                  <  2:765171/101‑1 (MQ=255)
                                                                                           ctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc               <  2:8570103/101‑1 (MQ=255)
                                                                                            tGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc              >  1:632138/1‑101 (MQ=255)
                                                                                                tCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg          >  2:2734303/1‑101 (MQ=255)
                                                                                                    g.gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgggggg      >  2:6579646/1‑100 (MQ=255)
                                                                                                    g.gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGc      <  1:1055972/101‑1 (MQ=40)
                                                                                                         gCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg  <  1:8274837/101‑1 (MQ=37)
                                                                                                         |                                                                                                      
GTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1434304‑1434504

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG