Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,405 C→G 20.9% intergenic (‑184/‑774) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4050C→G20.9% 289.0 ‑0.8 ‑0.3522intergenic (‑184/‑774)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (51/58):  ref base (176/237):  total (227/295)
Fisher's exact test strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.35e-01

Only 100 of 540 total aligned reads displayed.

TGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1434305‑1434505
                                                                                                         |                                                                                                      
 ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCg                                                                                                       <  1:7870985/101‑1 (MQ=255)
        aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAATC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCt                                                                                                <  1:6463448/101‑1 (MQ=255)
        aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCt                                                                                                >  1:4427476/1‑101 (MQ=255)
         aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                                               <  2:7867632/101‑1 (MQ=255)
         aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                                               <  2:7570654/101‑1 (MQ=255)
         aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGCCGCTCCCCCCGCGGCGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                                               >  1:9000528/1‑101 (MQ=255)
          cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..g                                                                                            <  1:1976671/101‑1 (MQ=255)
           aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..Gt                                                                                           <  1:4530900/101‑1 (MQ=255)
                 ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcc                                                                                     >  2:4867871/1‑101 (MQ=255)
                 ctTTAGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcc                                                                                     <  2:7979285/101‑1 (MQ=255)
                  tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTccc                                                                                    <  1:8345691/101‑1 (MQ=255)
                   ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccc                                                                                   <  2:8429031/101‑1 (MQ=255)
                             cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGc                                                                         <  1:1370762/101‑1 (MQ=255)
                               ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                                                       <  2:526765/101‑1 (MQ=255)
                               ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                                                       <  1:4489361/101‑1 (MQ=255)
                               ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                                                       <  2:40850/101‑1 (MQ=255)
                                cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTg                                                                      >  2:6222430/1‑101 (MQ=255)
                                 tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  1:7167006/1‑101 (MQ=255)
                                 tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTCCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  2:2589427/1‑101 (MQ=255)
                                    tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  >  2:1898524/1‑101 (MQ=255)
                                    cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  1:1142919/92‑1 (MQ=2)
                                    cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  1:5837120/92‑1 (MQ=2)
                                     gggctatcgTCGCTCCCCCCGCGGGAGCGTTGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGGTCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                    <  2:3265979/93‑1 (MQ=2)
                                     ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGCTCg                                                                 >  2:3744877/1‑101 (MQ=255)
                                     ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 <  1:3858817/101‑1 (MQ=255)
                                     ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 <  2:2195447/101‑1 (MQ=255)
                                      ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  2:6475276/94‑1 (MQ=2)
                                      ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  1:5524151/94‑1 (MQ=2)
                                      ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  2:6067518/94‑1 (MQ=2)
                                      ggctagcgTCGCTCCCCCCGCGGCGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  2:2935419/94‑1 (MQ=2)
                                      ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                >  2:9182526/1‑101 (MQ=255)
                                       tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               >  2:5377689/1‑101 (MQ=255)
                                       tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               >  1:5676098/1‑101 (MQ=255)
                                       gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  >  1:2038027/7‑101 (MQ=2)
                                        ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  2:5668430/6‑101 (MQ=2)
                                        ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 <  1:178397/96‑1 (MQ=2)
                                         gatcttCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                             <  1:8201281/96‑1 (MQ=255)
                                         ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                             <  1:1592449/101‑1 (MQ=255)
                                         ctgGGTCGCTCCCCCCGCGGCGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGCAac                                                             <  1:8820748/101‑1 (MQ=255)
                                          tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                            >  1:3307249/1‑101 (MQ=255)
                                          atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                               >  2:1729205/4‑101 (MQ=2)
                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                                          <  2:5517771/101‑1 (MQ=255)
                                            cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                             >  2:6349878/2‑101 (MQ=2)
                                            cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GGTTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                             <  2:3947337/100‑1 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                            >  1:5303036/1‑100 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                         <  2:1148668/101‑1 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgc                                                           <  2:2737897/101‑3 (MQ=2)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCCACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                                        <  2:4427349/101‑1 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                          >  1:6971326/1‑98 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                                       >  2:460174/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                      <  2:1530220/101‑1 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                      <  1:4052469/101‑1 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGCTTg                                                     >  2:7291494/1‑101 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                                     >  1:7614744/1‑101 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                                     <  2:7166702/101‑1 (MQ=255)
                                                 cTCCCCCAGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                                        >  2:3633396/1‑96 (MQ=2)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                                    >  1:4913538/1‑101 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                      >  2:2338575/1‑94 (MQ=2)
                                                   ccccccGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                      <  2:3140834/101‑8 (MQ=2)
                                                   ccccccGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                                   >  1:9128754/1‑101 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAAC..T..TGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                  <  2:888062/101‑8 (MQ=37)
                                                     ccccGCGGGGGCGTGGATCGAAAC..T..TGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                  >  2:7049686/1‑94 (MQ=37)
                                                      cccGCGGGGGCGTGGATCGAAACGGG..TCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                                   <  1:3693557/101‑11 (MQ=0)
                                                      cccGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                                <  1:6087041/101‑1 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                             >  2:5883562/1‑101 (MQ=255)
                                                          cgGGGGCGTGGATCGAAGC..G..TCTGCGCTCTGCTTCACG.GGTGCGTCCACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                            <  2:1091079/101‑1 (MQ=21)
                                                          cgGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                            <  2:1831073/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGTGGTGGATCGAAACACGGATTGCATgggtccg                                           >  1:7691344/1‑96 (MQ=255)
                                                           gggggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                           <  2:3455424/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                           >  1:8750597/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                           >  1:5147426/1‑101 (MQ=255)
                                                             gggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                         <  1:8528825/101‑1 (MQ=255)
                                                             gggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                         >  1:7487776/1‑101 (MQ=255)
                                                             gggCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                         >  1:7367438/1‑101 (MQ=255)
                                                                 gTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                     <  2:4965219/101‑1 (MQ=255)
                                                                  tGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                    <  1:3171976/101‑1 (MQ=255)
                                                                  tGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                    >  1:414131/1‑101 (MQ=255)
                                                                   ggATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                   >  1:6695722/1‑101 (MQ=255)
                                                                     aTCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc                                 >  1:2245958/1‑101 (MQ=255)
                                                                       cGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                               >  1:6277393/1‑101 (MQ=255)
                                                                       cGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                               <  2:9048746/101‑1 (MQ=255)
                                                                        gAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                              >  2:2118679/1‑101 (MQ=255)
                                                                         aaaC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                             <  2:6924661/101‑1 (MQ=255)
                                                                         aaaC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                             <  1:79879/101‑1 (MQ=255)
                                                                          aaC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                            >  1:2610157/1‑101 (MQ=255)
                                                                           aC..G..ACTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCATTGcg                           >  2:823431/1‑100 (MQ=255)
                                                                            c..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACAGATTGCATGGCGCCGAGGGACAGGCTTTGCAg                          >  2:6750936/1‑101 (MQ=255)
                                                                               g..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                         >  1:6008392/1‑101 (MQ=255)
                                                                                  tctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                        <  1:4806477/101‑1 (MQ=255)
                                                                                  tctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                        <  2:22564/101‑1 (MQ=255)
                                                                                       gCTCTGCTTCAAG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                   <  2:765171/101‑1 (MQ=255)
                                                                                         tctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc                 >  1:2836979/1‑101 (MQ=255)
                                                                                         tctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc                 <  2:4534157/101‑1 (MQ=255)
                                                                                          ctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                <  2:8570103/101‑1 (MQ=255)
                                                                                           tGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc               <  2:1429836/101‑1 (MQ=255)
                                                                                           tGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc               >  1:632138/1‑101 (MQ=255)
                                                                                              ttCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgcg            >  2:8771545/1‑101 (MQ=255)
                                                                                                   g.gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgggggg       >  2:6579646/1‑100 (MQ=255)
                                                                                                   g.gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGc       <  1:1055972/101‑1 (MQ=40)
                                                                                                       tGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTg    <  1:343562/101‑1 (MQ=38)
                                                                                                         |                                                                                                      
TGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..G..TCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1434305‑1434505

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG