Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,413 T→G 21.3% intergenic (‑192/‑766) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4130T→G21.3% 290.5 ‑0.8 ‑0.0508intergenic (‑192/‑766)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (50/58):  ref base (168/230):  total (219/289)
Fisher's exact test strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.54e-01

Only 100 of 533 total aligned reads displayed.

AACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434313‑1434510
                                                                                                       |                                                                                                   
aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCt                                                                                                     >  1:7445536/1‑101 (MQ=255)
 aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                                                    <  2:7867632/101‑1 (MQ=255)
 aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                                                    <  1:782941/101‑1 (MQ=255)
 aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCCCG.GCTGCGTCAACCtt                                                                                                    >  1:6568961/1‑101 (MQ=255)
  cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..g                                                                                                 >  2:110938/1‑101 (MQ=255)
  cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..g                                                                                                 <  1:1976671/101‑1 (MQ=255)
    ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTc                                                                                               >  1:6014864/1‑101 (MQ=255)
     gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCg                                                                                              <  1:5649584/101‑1 (MQ=255)
        gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTc                                                                                           >  1:9064693/1‑101 (MQ=255)
        gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTc                                                                                           >  2:7051810/1‑101 (MQ=255)
           ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTGTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccc                                                                                        <  1:8199213/101‑1 (MQ=255)
             gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccccc                                                                                      <  1:5766408/101‑1 (MQ=255)
                   aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCgggg                                                                                <  1:5103982/101‑1 (MQ=255)
                   aaCACCTGAGCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCgggg                                                                                <  2:6939275/101‑1 (MQ=255)
                     cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGc                                                                              <  1:1370762/101‑1 (MQ=255)
                      acCAGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCggggggg                                                                             >  1:7213238/1‑99 (MQ=255)
                        cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTg                                                                           <  2:3630391/101‑1 (MQ=255)
                        cTGATCCTGCTGGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCCT..GTCGCTCCCCCCGCGGGGGCGTg                                                                           <  2:1028520/101‑1 (MQ=255)
                           aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:1570553/101‑1 (MQ=255)
                           aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGGgggt                                                                        >  2:3253966/1‑99 (MQ=255)
                            tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  2:1898524/1‑101 (MQ=255)
                            cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  2:1729939/92‑1 (MQ=2)
                            cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  1:1142919/92‑1 (MQ=2)
                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGCTCg                                                                      >  2:3744877/1‑101 (MQ=255)
                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:2689756/101‑1 (MQ=255)
                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:2195447/101‑1 (MQ=255)
                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:3858817/101‑1 (MQ=255)
                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:4130292/101‑1 (MQ=255)
                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:4335561/101‑1 (MQ=255)
                              ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:6010675/94‑1 (MQ=2)
                              ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  2:234533/101‑1 (MQ=255)
                              ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     >  2:9182526/1‑101 (MQ=255)
                               gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGGTc                                                                       >  1:4488511/7‑101 (MQ=2)
                               gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  1:6290795/95‑1 (MQ=2)
                               gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  1:2038027/7‑101 (MQ=2)
                                ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:6328208/96‑1 (MQ=2)
                                ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:1204716/96‑1 (MQ=2)
                                ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:178397/96‑1 (MQ=2)
                                 ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  1:4761698/101‑1 (MQ=255)
                                 ctgGGTCGATCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGATTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  2:5403705/101‑1 (MQ=255)
                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 >  1:3307249/1‑101 (MQ=255)
                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 <  1:2631443/101‑1 (MQ=255)
                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 >  1:8311215/1‑101 (MQ=255)
                                  atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  1:2309858/4‑101 (MQ=2)
                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                >  2:5639638/1‑101 (MQ=255)
                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                <  1:5887868/101‑1 (MQ=255)
                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                <  2:5781825/101‑1 (MQ=255)
                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  >  2:6347948/2‑101 (MQ=2)
                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  1:488163/100‑1 (MQ=2)
                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGGGGATCGAAac                                                                  >  2:6381122/2‑101 (MQ=2)
                                    cgTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  2:6537707/100‑1 (MQ=2)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGCAacg                                                                 >  2:2843159/1‑100 (MQ=2)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                              <  2:314364/101‑1 (MQ=255)
                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                                             >  2:6131633/1‑101 (MQ=255)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                               >  2:1466542/1‑98 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                              <  1:8925248/101‑5 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                              >  1:4092651/1‑97 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                           <  2:8781499/101‑1 (MQ=255)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                           >  2:79879/1‑101 (MQ=255)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                                             <  1:6906154/101‑6 (MQ=2)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                            >  1:5266945/1‑95 (MQ=2)
                                           ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                           >  1:8787993/1‑94 (MQ=2)
                                           ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                           >  1:8569130/1‑94 (MQ=2)
                                           ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                                        >  2:4896142/1‑101 (MQ=255)
                                            cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                                       <  2:8590553/101‑1 (MQ=255)
                                             ccccGCGGGGGCGTGGATCGAAAC..TTGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                       >  2:720983/1‑94 (MQ=37)
                                              cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                                     <  2:5292689/101‑1 (MQ=255)
                                              cacgggggggCGTGGAGCGAAGC..GTCTCCGCTCTTCTTCCCG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                                     <  2:2943857/96‑1 (MQ=21)
                                                  cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  1:3702824/101‑1 (MQ=255)
                                                  cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  1:5521233/101‑1 (MQ=255)
                                                  cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  2:1831073/101‑1 (MQ=255)
                                                  cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 >  1:7052891/1‑101 (MQ=255)
                                                   gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                                <  2:3455424/101‑1 (MQ=255)
                                                    ggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCGGATTGCATGGCGCCGa                                               >  1:6195534/1‑101 (MQ=255)
                                                       gCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                                            <  2:3954443/101‑1 (MQ=255)
                                                        cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                           >  1:7984749/1‑101 (MQ=255)
                                                          tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                         <  1:3171976/101‑1 (MQ=255)
                                                          tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                         <  2:6752850/101‑1 (MQ=255)
                                                           ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                        >  2:47265/1‑101 (MQ=255)
                                                               cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                    <  2:9048746/101‑1 (MQ=255)
                                                                gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   >  2:2118679/1‑101 (MQ=255)
                                                                   aC..GACTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCATTGcg                                >  2:823431/1‑100 (MQ=255)
                                                                       gTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                              <  2:4445949/101‑1 (MQ=255)
                                                                        tctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                             <  2:22564/101‑1 (MQ=255)
                                                                         ctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg                            <  1:4519346/101‑1 (MQ=255)
                                                                             gCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                        <  1:7602872/101‑1 (MQ=255)
                                                                             gCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                        <  1:8420164/101‑1 (MQ=255)
                                                                              ctctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccc                       <  1:7689059/101‑1 (MQ=255)
                                                                               tctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc                      >  2:7315799/1‑101 (MQ=255)
                                                                                ctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                     <  2:8498379/101‑1 (MQ=255)
                                                                                ctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                     >  2:4542319/1‑101 (MQ=255)
                                                                                  gCTTCACG.GCTGCGTCAACCTT..GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                   <  2:5147257/101‑1 (MQ=255)
                                                                                     tCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg                >  2:7638701/1‑101 (MQ=255)
                                                                                     tCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGAGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg                >  1:1564817/1‑101 (MQ=255)
                                                                                         g.gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGc            <  1:1055972/101‑1 (MQ=40)
                                                                                                 tCACCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:8910225/101‑1 (MQ=11)
                                                                                                 tCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:7575545/101‑1 (MQ=33)
                                                                                                  cAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  1:2846586/101‑1 (MQ=32)
                                                                                                  cAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  1:3477276/101‑1 (MQ=32)
                                                                                                   aaCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGCAGATcct   <  2:6669412/101‑3 (MQ=9)
                                                                                                       |                                                                                                   
AACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434313‑1434510

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG