Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,447 A→T 2.0% intergenic (‑226/‑732) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4470A→T 2.0% 6.0 0.0 ‑0.7256intergenic (‑226/‑732)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (103/147):  total (106/150)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.15e-01

Only 100 of 280 total aligned reads displayed.

TGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434347‑1434510
                                                                                                      |                                                               
tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 >  2:9187775/1‑101 (MQ=255)
tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 >  1:8311215/1‑101 (MQ=255)
 gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                <  1:6925140/101‑1 (MQ=255)
 gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                <  1:5887868/101‑1 (MQ=255)
 gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                <  1:2006417/101‑1 (MQ=255)
   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                              <  2:314364/101‑1 (MQ=255)
   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                              <  2:1148668/101‑1 (MQ=255)
   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                              <  2:7057587/101‑1 (MQ=255)
    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCCACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                                             <  2:4427349/101‑1 (MQ=255)
    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacagatc                                                             >  2:8200932/1‑97 (MQ=255)
     cGCTCCCCCCGCGGGGGCGTGGATCGNAACNTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                                            <  1:5788804/101‑1 (MQ=255)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                           >  2:79879/1‑101 (MQ=255)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                           <  1:3306437/101‑1 (MQ=255)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                           <  2:4584378/101‑1 (MQ=255)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                           <  1:4052469/101‑1 (MQ=255)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                           <  2:5664748/101‑1 (MQ=255)
       cTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                                          >  1:7614744/1‑101 (MQ=255)
        tCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                                         >  2:3283867/1‑101 (MQ=255)
         ccccccGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                                        >  1:8987469/1‑101 (MQ=255)
          cccccGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                                       <  1:9188197/101‑1 (MQ=255)
          cccccGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                                       <  2:2895605/101‑1 (MQ=255)
           ccccGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                       >  2:720983/1‑94 (MQ=37)
           ccccGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaacgac                                                       <  1:8791791/101‑8 (MQ=37)
           ccccGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                                      <  1:4948623/101‑1 (MQ=255)
           ccccGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                                      <  1:6428934/101‑1 (MQ=255)
             ccGCGGGGGCGTGGATCGAAACGGCTCCGCTCTGCTTCACGGCTGCGGCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                    <  2:9000106/101‑1 (MQ=255)
               gcgGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                                  >  2:5883562/1‑101 (MQ=255)
                cgGGGGCGTGGATCGAAGCGTCTGCGCTCTGCTTCACGGGTGCGTCCACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  2:1091079/101‑1 (MQ=21)
                cgGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATggaggc                                                 >  1:4219941/1‑97 (MQ=255)
                cgGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 >  2:5806103/1‑101 (MQ=255)
                cgGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 >  1:3674258/1‑101 (MQ=255)
                 gggggCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                                <  2:3455424/101‑1 (MQ=255)
                 gggggCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                                >  1:5147426/1‑101 (MQ=255)
                  ggggCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCGGATTGCATGGCGCCGa                                               >  1:6195534/1‑101 (MQ=255)
                   gggCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                              >  1:7487776/1‑101 (MQ=255)
                    ggCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTTGCTCCCCCCGCGGGGGCGTCGATCGAAACACGGATTGCATGGCGCCGAgg                                             >  2:1599192/1‑101 (MQ=255)
                    ggCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                                             <  1:5414358/101‑1 (MQ=255)
                    ggCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                                             <  1:2734648/101‑1 (MQ=255)
                     gCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                                            <  2:3954443/101‑1 (MQ=255)
                       gTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                          <  2:4965219/101‑1 (MQ=255)
                       gTGGATCGAAACGGCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                          <  1:4125388/101‑1 (MQ=255)
                        tGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                         <  1:5674256/101‑1 (MQ=255)
                        tGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                         <  2:6752850/101‑1 (MQ=255)
                        tGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                         <  2:2331908/101‑1 (MQ=255)
                         ggATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                        >  2:4482443/1‑101 (MQ=255)
                         ggATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                        >  2:47265/1‑101 (MQ=255)
                         ggATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                        <  1:604482/101‑1 (MQ=255)
                         ggATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCCCGGCGGCGTGGATCGAAACACTGAGTGCATGGCGCGGGGGGGCAg                                        >  1:1404722/1‑101 (MQ=25)
                           aTCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc                                      <  2:1326673/101‑1 (MQ=255)
                             cGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                    >  1:6277393/1‑101 (MQ=255)
                             cGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                    >  1:1968616/1‑101 (MQ=255)
                             cGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                    >  1:107803/1‑101 (MQ=255)
                              gatctgTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  1:84865/96‑1 (MQ=255)
                              gAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   >  2:2118679/1‑101 (MQ=255)
                              gAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   >  2:1476317/1‑101 (MQ=255)
                              gAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   >  1:4819525/1‑101 (MQ=255)
                              gAAACGGCTCCGCTCTTCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  1:8696868/101‑1 (MQ=255)
                               aaaCGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                                  <  1:79879/101‑1 (MQ=255)
                                aaCGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 <  2:8805010/101‑1 (MQ=255)
                                aaCGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 >  2:5313773/1‑101 (MQ=255)
                                aaCGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 >  1:7878248/1‑101 (MQ=255)
                                 aCGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa                                <  1:3241612/101‑1 (MQ=255)
                                 aCGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa                                <  1:7062775/101‑1 (MQ=255)
                                   gTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtttagatc                              >  2:84865/1‑96 (MQ=255)
                                    tctcCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                             <  2:6464249/101‑1 (MQ=255)
                                     ctcCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg                            <  1:4519346/101‑1 (MQ=255)
                                     ctcCGATCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg                            <  1:2539399/101‑1 (MQ=255)
                                       ccGCTCTGCTTCACGGCTTCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCt                          <  2:7691018/101‑1 (MQ=255)
                                        cGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc                         <  2:1972192/101‑1 (MQ=255)
                                        cGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc                         <  2:8890638/101‑1 (MQ=255)
                                        cGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc                         <  2:8612867/101‑1 (MQ=255)
                                          ctctGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccc                       <  1:7689059/101‑1 (MQ=255)
                                           tctGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc                      >  2:7315799/1‑101 (MQ=255)
                                            ctGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                     <  2:8570103/101‑1 (MQ=255)
                                             tGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                    >  1:632138/1‑101 (MQ=255)
                                             tGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                    <  2:2245891/101‑1 (MQ=255)
                                             tGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                    <  2:6877831/101‑1 (MQ=255)
                                              gCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                   <  2:7033866/101‑1 (MQ=255)
                                                ttCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgcg                 >  2:8771545/1‑101 (MQ=255)
                                                ttCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgcg                 >  2:6329115/1‑101 (MQ=255)
                                                 tCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg                >  2:2734303/1‑101 (MQ=255)
                                                 tCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg                >  2:7638701/1‑101 (MQ=255)
                                                 tCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGAGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg                >  1:1564817/1‑101 (MQ=255)
                                                    cGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCggggg             >  1:1776264/1‑101 (MQ=255)
                                                     ggCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgggggg            >  2:6579646/1‑100 (MQ=255)
                                                     ggCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGc            <  1:1055972/101‑1 (MQ=40)
                                                      gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCg           >  2:173650/1‑101 (MQ=40)
                                                      gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCg           >  2:220581/1‑101 (MQ=40)
                                                        tGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTg         <  1:343562/101‑1 (MQ=38)
                                                         gCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg        >  1:521676/1‑101 (MQ=37)
                                                         gCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg        <  1:8274837/101‑1 (MQ=37)
                                                         atctCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg        <  2:3824591/98‑1 (MQ=18)
                                                          cGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGCCAGGCTTGGCAGTCGCTCCCCCCGCGGGGGCGTGGa       >  1:2963334/1‑101 (MQ=255)
                                                            tCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:6125409/101‑1 (MQ=33)
                                                             cAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGagat    >  1:3824700/1‑98 (MQ=17)
                                                             cAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  2:4913378/101‑1 (MQ=32)
                                                              aaCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   >  1:8398743/1‑101 (MQ=31)
                                                              aaCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   >  2:3192172/1‑101 (MQ=31)
                                                               aCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  2:6714320/101‑1 (MQ=11)
                                                               aCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  >  2:524220/1‑101 (MQ=11)
                                                                                                      |                                                               
TGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCTCCGCTCTGCTTCACGGCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434347‑1434510

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG