Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,612,428 T→C 2.5% intergenic (+106/‑255) purN → / → rnd2 phosphoribosylglycinamide formyltransferase/ribonuclease D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,612,4280T→C 2.5% 12.5 ‑3.7 ‑0.3401intergenic (+106/‑255)purN/rnd2phosphoribosylglycinamide formyltransferase/ribonuclease D
Reads supporting (aligned to +/- strand):  new base (9/1):  ref base (209/182):  total (218/183)
Fisher's exact test strand distribution p-value = 2.46e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.89e-01

Only 100 of 429 total aligned reads displayed.

ATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGGCGGGGAAAGGCGA  >  NC_014034/1612328‑1612528
                                                                                                    |                                                                                                        
aTCCGGTGAGCTGCCCGAGGCCCTCCCCGAGGTCCCGCGACCCTTGGGCCCCGTTCCCGCCGAGGCCCCAGCGGCCCTCGGCCTGCGCCCGCCCCGCCCAt                                                                                                          <  2:8604396/101‑1 (MQ=12)
aTCCGCTGATCTGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCAt                                                                                                          <  1:435436/101‑1 (MQ=255)
 tCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATg                                                                                                         >  1:185979/1‑101 (MQ=255)
 tCCGCTGATCGGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATg                                                                                                         <  2:1814301/101‑1 (MQ=255)
 cccGCTGATCTGCCCGGGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATg                                                                                                         <  2:3690742/100‑1 (MQ=255)
  ccGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATgg                                                                                                        >  1:5768419/1‑101 (MQ=255)
   cGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggc                                                                                                       >  1:9028098/1‑101 (MQ=255)
    gCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcg                                                                                                      >  2:2314782/1‑101 (MQ=255)
      tGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcggg                                                                                                    >  2:6657896/1‑101 (MQ=255)
      tGATCGGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcggg                                                                                                    <  1:4091393/101‑1 (MQ=255)
       gATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcgggg                                                                                                   >  1:4617683/1‑100 (MQ=255)
       gATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcgggc                                                                                                   <  1:6005231/101‑1 (MQ=255)
         tCTGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGgcgc                                                                                                 <  1:4770734/101‑1 (MQ=255)
           tgcccGAGGCCATCGCCGAGGTCCCGCGACACATTGCCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCAt                                                                                               <  1:8170178/98‑1 (MQ=255)
           tGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCAt                                                                                               >  2:1980900/1‑101 (MQ=255)
           ggACCGAGCCCATCCCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCAt                                                                                               <  2:2163433/100‑1 (MQ=255)
            gcccGAGCCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCAtt                                                                                              <  2:8395570/99‑1 (MQ=255)
             aCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcgggcggcttg                                                                                             >  1:8833504/1‑96 (MQ=255)
              ccGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGgggccctcc                                                                                            >  2:8205779/1‑96 (MQ=255)
              ccGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGGCTTcg                                                                                            >  2:3346714/1‑101 (MQ=255)
               cGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTcgc                                                                                           >  1:265757/1‑101 (MQ=255)
               cGAGGCCATCGCCGAGGGCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcgggccggctagc                                                                                           >  2:9128506/1‑93 (MQ=255)
               cGAGGCCATCCCCGAGGTCCCGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTcgc                                                                                           <  1:1483123/101‑1 (MQ=255)
                gAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCCTTcgc.g                                                                                         >  1:126749/1‑101 (MQ=255)
                gAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCACTcgc.g                                                                                         >  1:2053041/1‑101 (MQ=255)
                 aGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGACCCCGCACAGCACATggcgcgcaggccggcca                                                                                         >  1:4196139/1‑91 (MQ=17)
                  ggCCATTGCCGAGGTCAAGCG.CACCCTCTCCCCGTGACCGCCGAGGGCCCCAAGGCACTCGGCCAGCGCCCGCCCCGCCCATGGCGGTTTCATTCCc.g.ccg                                                                                     >  2:6013215/1‑101 (MQ=18)
                   gCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGc.g.ccc                                                                                     >  1:586664/1‑100 (MQ=255)
                   gCCATCCCCGAGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGc.g.ccg                                                                                     <  2:8022315/101‑1 (MQ=255)
                    ccATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCagttccgcccc                                                                                      >  2:4534077/1‑91 (MQ=255)
                    ccATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGc.g.cccc                                                                                    >  1:2493005/1‑99 (MQ=255)
                      accGCCGAGGCCCCGCGACACATTGCCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.ccgccg                                                                                  <  2:5337512/99‑1 (MQ=255)
                        cGCCGAGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCggg                                                                                <  1:3392770/101‑1 (MQ=255)
                           cGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCCTCGC.G.CCGCCCGGGCg                                                                             >  1:5500800/1‑101 (MQ=255)
                             gggccccgcgACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCggg                                                                           <  1:7696211/95‑1 (MQ=255)
                               gtcgccccacacATTGGCCCCGTGCCCGCGGAGGGCCCGGGGCCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGAc                                                                         <  2:4019913/93‑1 (MQ=17)
                                tcccgcgACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACg                                                                        <  1:7157697/98‑1 (MQ=255)
                                 cACGCGACACATTGGCCCCGTGCCCGCCGAGGCCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGc                                                                       <  2:5071006/101‑1 (MQ=255)
                                 cACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGTGGGCATTCGC.G.CCGCCCGGGCggggggc                                                                       >  1:7963483/1‑97 (MQ=17)
                                  aCGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGACCATGGCGGGGGCGTTCGC.G.CCCCCCGGGCggggggcc                                                                      >  1:6557007/1‑96 (MQ=17)
                                     cgaccaaTTGGCCCCGTGCCCGCCGAGGGCCCAGGGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGG.G.GCGCCGGGGCGGGACGCAGGc                                                                   <  2:3599699/95‑1 (MQ=17)
                                     cgACACATGGGCCCCGTGCCGGCCGAGGCCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGc                                                                   <  1:3343391/101‑1 (MQ=17)
                                      gACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTTGC.G.CCGCCCGGGCGGGGCGGAGGcc                                                                  >  2:3780338/1‑101 (MQ=17)
                                        cacaTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGC.G.CCGCCCGGGCGGGGCGCCGGcccc                                                                >  1:191405/1‑99 (MQ=14)
                                        cacaTGGGCCCCGTGCCCGCGGAGGGCCCAGAGCCCCTCGCCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTc                                                                <  1:8388873/101‑1 (MQ=17)
                                          cttgggCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTccc                                                              <  2:4327378/97‑1 (MQ=255)
                                          caTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTccc                                                              <  2:7586851/101‑1 (MQ=255)
                                          caTTGCCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTccc                                                              <  2:315566/101‑1 (MQ=255)
                                          caTGGGCCCCGTGACCGCCGGGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTccc                                                              <  1:1640252/101‑1 (MQ=255)
                                            tgggCCCCGTGCCCGCCGAGGGCCCAGAGCCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCcgc                                                            <  1:5303221/99‑1 (MQ=255)
                                               gCCCCGTGACCGGCGAGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGCGGCTGC.G.CCGCCCCGGCGGGGCGCAGGGCCcccttgtg                                                         >  1:3215356/1‑96 (MQ=14)
                                                  ccgggcccgccgAGGGCCGAGAGCCCCCCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.tt                                                     <  2:3048126/95‑1 (MQ=17)
                                                  ccGTGACCGCGGGGGGCCCTGGGCCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.tt                                                     <  1:5138780/101‑1 (MQ=17)
                                                   cGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGGGGGGGGCCGGCCCCCCCCGCGC.GTg                                                    >  2:846831/1‑101 (MQ=14)
                                                    gTGACCGCCGGGGCCCCAGAGCCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTgg                                                   <  2:7235184/101‑1 (MQ=255)
                                                    gTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCATTTGC.G.CCCCCCGGGCGGGACGCAGGCCTCCCCCGCGC.CTgg                                                   >  2:779602/1‑101 (MQ=17)
                                                    gTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCCC.G.CCGCCCGGGCGGGGCGCGGGCCTCCCgcgcgcgcgg                                                    >  2:4907385/1‑97 (MQ=14)
                                                     ggcccgccgGGGGCCCAGGGGCCCTCGGCCTGCCCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTggg                                                  <  1:7027040/98‑1 (MQ=18)
                                                      gcccgccgGGGGCCCAGAGGCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTgggg                                                 <  2:6502997/99‑1 (MQ=255)
                                                        ccgcggAGGCCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAg                                               <  2:7830485/101‑1 (MQ=255)
                                                            cgggggCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGGCTTCGC.GCCCCCCGGGGCGGGGGGGGGGCC.CCCGCGCGCGTTGGGGaaagg                                            >  1:979794/4‑97 (MQ=2)
                                                             gAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGGGGGACGCAGGCCTCCCGCGCGC.TTGGGGGGGCggt                                          >  2:738085/1‑99 (MQ=255)
                                                             gAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGGCGCAGGGCTCCCCCGCGC.TTGGGGGgggggt                                          >  1:3723842/1‑97 (MQ=14)
                                                             gAGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGGCGTTGC.G.CCGCCCCGGCGGGGGGGGGCCCGCCCGGGCGC.TTtggggacacgg                                          >  2:1431675/1‑94 (MQ=2)
                                                              aGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCCGGGCGGGGCGCAAGCCCCCCGCGCGC.TTGGGGGGGCGCTc                                         >  2:5983845/1‑101 (MQ=17)
                                                               gggCCCAGGGGCCGTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCt                                        <  2:6173975/101‑1 (MQ=255)
                                                                gccccgggggCCCTCGCCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCtt                                       <  2:5219265/93‑1 (MQ=255)
                                                                 gCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTg                                      <  1:738098/101‑1 (MQ=255)
                                                                    cAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.GCGCCGTGGGGGGGCGCAGGCCTCCCCGCCGC.CTGGGGGGGGGaggtggtta                                   >  2:2931519/1‑93 (MQ=2)
                                                                     agagCCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCAc                                  <  2:3534400/101‑1 (MQ=255)
                                                                      gagccgcTCGGCCTGCCCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCAcc                                 <  2:592148/95‑1 (MQ=255)
                                                                      gagGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCATTCGC.G.CCGCCGGGGCGGGGCGCCGGGCCCCCGCCCGC.TTGGGGGGGGGGTCTTGtgccc                                 >  2:7002514/1‑97 (MQ=14)
                                                                       agtcccTCGGCCTGGGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCg                                <  2:9059881/98‑1 (MQ=255)
                                                                       agGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCCCGCGC.CTGGGGGGGCGGTCTTTTCACCg                                >  2:8113073/1‑101 (MQ=17)
                                                                         gCCCCCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGc                              <  1:2819126/101‑1 (MQ=255)
                                                                           cgtgccctgccgcccgccCCGCCCATGGCGCGCGCATTCTC.G.CCGCCGGGGGGGGACGCAGTCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCtt                            <  1:3893649/91‑1 (MQ=21)
                                                                           ccTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCATTTGCGG.CCGCCGGGGCGGGAGGAAGGCCCCCCCCCCGC.TCGGGGGGGGGTGCTTGttcccgcct                             >  2:1133271/1‑93 (MQ=9)
                                                                           ccTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCtt                            <  1:4939578/101‑1 (MQ=255)
                                                                            ctcgcctggcgcCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTg                           <  2:8924381/93‑1 (MQ=255)
                                                                             tCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGGGATCTTGTCCCCGGCtttg                          >  2:2757407/1‑99 (MQ=255)
                                                                             gcGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTgg                          <  1:3034958/100‑1 (MQ=255)
                                                                              cGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGGGCTCTTGTCACCGGCTTTGt                         >  2:4143803/1‑101 (MQ=255)
                                                                               ggCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTc                        <  1:2312065/101‑1 (MQ=255)
                                                                                gCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGGGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCCTTTCACCGGCTTGGTcg                       >  2:3822304/1‑101 (MQ=255)
                                                                                  cTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTcgc.g                    <  2:5488468/101‑1 (MQ=255)
                                                                                  cTGCCCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTcgc.g                    <  1:2933478/101‑1 (MQ=255)
                                                                                  cTGCCCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTcgc.g                    <  1:6845945/101‑1 (MQ=255)
                                                                                   tGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGGCGCAGGCCTCCCGCGCGC.TTGGGGGGGCGATCTTGTCACCGGCTTGGTcgggc                    >  1:7274264/1‑98 (MQ=255)
                                                                                    gcgcCCGCCCCGCCCATGGCGGGCGCCTTCGC.G.CCGCCGGGGGGGGGCGCCGGCCTCCCGCGCGC.TTGGGGGGGCGGTCTTGTCACCGGCTTTGGcgc.ggc                  >  1:569829/1‑99 (MQ=17)
                                                                                    gcgcCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GAc                  <  2:8738169/101‑1 (MQ=255)
                                                                                    ccccccgccccgcCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GAc                  <  1:8610290/98‑1 (MQ=255)
                                                                                     cccccgccccgcCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GAcg                 <  1:6277522/99‑1 (MQ=255)
                                                                                      gcccgcccCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GAcgg                <  2:5541409/101‑1 (MQ=255)
                                                                                       cccgccccgcCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.CTGGGGGGGGGAGCTTGTCACCGGCTTTGTCGC.CGcggg               >  1:781785/1‑101 (MQ=21)
                                                                                           ccccgcccATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGGCTCCCGCGCGC.CTGGGGAGGCGATCTTGTGACCGGCTTTGTCCCGGAcgggcgg            >  2:6851972/1‑101 (MQ=21)
                                                                                               gcccATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGGCGGGGaaa       <  2:5730051/101‑1 (MQ=255)
                                                                                               gcccATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGGCGGGGaaa       <  1:2373824/101‑1 (MQ=255)
                                                                                               gcccATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCCGCTTTGTCGC.CGCGGGCgggggga       >  2:2816259/1‑98 (MQ=25)
                                                                                                cccATGGCGGGCGCATTCGC.G.CCGCCGGGGGGGGGCGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTTGGCGC.GGCGGGGGGGGGAAg      >  1:1050822/1‑101 (MQ=21)
                                                                                                   aTGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGGCGGGGAAAGGCg   <  2:3464053/101‑1 (MQ=255)
                                                                                                    |                                                                                                        
ATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGC.G.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGC.TTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGGCGGGGAAAGGCGA  >  NC_014034/1612328‑1612528

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG