Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,183 C→T 4.2% intergenic (‑111/+114) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1830C→T 4.2% 37.8 0.0 ‑1.4456intergenic (‑111/+114)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (10/9):  ref base (221/216):  total (231/225)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.40e-02

Only 100 of 482 total aligned reads displayed.

CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.G.G.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCC  >  NC_014034/1696082‑1696285
                                                                                                        |                                                                                                       
 cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCTG.C.CAATTca                                                                                                        >  1:5553873/1‑100 (MQ=255)
  aaaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTcac                                                                                                       <  1:4653035/101‑3 (MQ=255)
    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.CCAATTCA.ccc                                                                                                   <  2:2536666/101‑1 (MQ=255)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.CCAATTCA.cccc                                                                                                  <  1:645836/101‑2 (MQ=37)
       gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.CCAATTCA.CCCcgg                                                                                                >  1:8575659/1‑101 (MQ=37)
         cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGa                                                                                               <  2:162687/101‑1 (MQ=255)
         cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGa                                                                                               >  2:6180606/1‑101 (MQ=255)
         cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGa                                                                                               <  2:5858495/101‑1 (MQ=255)
          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGag                                                                                              >  2:5315697/1‑100 (MQ=37)
           gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGatg                                                                                             >  2:241690/1‑99 (MQ=255)
            aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGatgt                                                                                            <  1:1333073/101‑4 (MQ=255)
             aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGatgtt                                                                                           <  2:2847999/101‑5 (MQ=255)
              ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGatgttt                                                                                          <  2:2165913/101‑6 (MQ=255)
              ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGatgttt                                                                                          >  2:1139305/1‑96 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTg                                                                                         <  2:7170808/101‑1 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTg                                                                                         <  2:357226/101‑1 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTg                                                                                         >  2:3765442/1‑101 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTg                                                                                         <  1:2194374/101‑1 (MQ=255)
                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTgg                                                                                        >  1:4597083/1‑101 (MQ=25)
                 aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTggg                                                                                       >  2:4817701/1‑101 (MQ=255)
                    gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCa                                                                                    >  1:7010184/1‑101 (MQ=255)
                      cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaag                                                                                  >  2:4185393/1‑100 (MQ=21)
                        gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                                <  1:4046330/101‑4 (MQ=255)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                               <  2:6389652/101‑5 (MQ=255)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                               <  1:3171981/101‑5 (MQ=255)
                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga                                                                              >  1:7179833/1‑96 (MQ=255)
                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga                                                                              >  2:1263989/1‑96 (MQ=255)
                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa                                                                             <  1:3432965/101‑7 (MQ=255)
                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCGCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGCTGTTTGGGCCaaggcgaag                                                                            >  1:4983160/1‑94 (MQ=255)
                             aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAac                                                                         <  1:6180845/101‑1 (MQ=16)
                             aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAac                                                                         >  2:4623506/1‑101 (MQ=255)
                              tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                        >  2:1809254/1‑100 (MQ=255)
                              tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                        <  1:3918602/101‑2 (MQ=255)
                               ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                       <  2:1979051/101‑3 (MQ=255)
                               ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaa                                                                       >  1:528919/1‑99 (MQ=12)
                                tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GCTT.CCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                      <  1:8962488/101‑4 (MQ=16)
                                 cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                     <  1:8306843/101‑5 (MQ=255)
                                 cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                     >  1:474095/1‑97 (MQ=255)
                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                    >  2:8271786/1‑96 (MQ=12)
                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                    >  1:776384/1‑96 (MQ=12)
                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                    <  2:4512623/101‑6 (MQ=255)
                                    aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                  >  2:7690978/1‑94 (MQ=255)
                                     tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                 <  1:4390118/101‑9 (MQ=255)
                                       cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTTGGCCAA..GGTGAAacaccttgcttt                                                               >  2:4543699/1‑91 (MQ=255)
                                       cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                               <  2:6073741/101‑11 (MQ=255)
                                          gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                          <  2:3249574/101‑1 (MQ=255)
                                           cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                         <  1:1745840/101‑1 (MQ=255)
                                           cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                         <  1:1117682/101‑1 (MQ=255)
                                            aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                        <  2:2335744/101‑1 (MQ=9)
                                            aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                        <  1:2400185/101‑1 (MQ=9)
                                              gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                      >  1:4839558/1‑101 (MQ=9)
                                               aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCGC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                     <  1:927419/101‑1 (MQ=9)
                                               aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GCTGAAA.CACCTT.GCTTTTTCTTGgc                                                     <  2:4371030/101‑1 (MQ=9)
                                                  tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                  >  1:5975322/1‑101 (MQ=255)
                                                   ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                 >  1:953557/1‑101 (MQ=255)
                                                   ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                 >  2:5131253/1‑101 (MQ=255)
                                                   ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                 <  1:6827683/101‑1 (MQ=255)
                                                   ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGACAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                 >  1:3331828/1‑101 (MQ=255)
                                                    tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                <  2:92221/101‑1 (MQ=255)
                                                    tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                <  1:186579/101‑1 (MQ=255)
                                                     gCAAGGATGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               <  1:5827168/101‑1 (MQ=9)
                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               >  1:6702561/1‑101 (MQ=255)
                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               <  1:4277184/101‑1 (MQ=9)
                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               >  1:5488121/1‑101 (MQ=255)
                                                     gCAAGGAGGGGAGAGGGGC..CCTGCCCCGCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               <  2:7048738/101‑1 (MQ=9)
                                                      cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                              >  2:6671757/1‑101 (MQ=255)
                                                      cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                              >  2:8145966/1‑101 (MQ=255)
                                                      cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                              <  2:4739872/101‑1 (MQ=255)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                             >  1:7952831/1‑101 (MQ=9)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                             <  2:1723835/101‑1 (MQ=255)
                                                        aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                            >  1:4444059/1‑101 (MQ=255)
                                                        aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                            >  2:2688379/1‑101 (MQ=255)
                                                        aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                            >  1:2963891/1‑101 (MQ=255)
                                                         ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                           >  2:3435811/1‑101 (MQ=255)
                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                         <  2:6914665/101‑1 (MQ=9)
                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                         <  1:5721986/101‑1 (MQ=9)
                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                         <  1:1576521/101‑1 (MQ=9)
                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                         >  2:7878682/1‑101 (MQ=12)
                                                            ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                        >  1:7512077/1‑101 (MQ=12)
                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                       <  2:8629141/101‑1 (MQ=255)
                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                       <  2:8804757/101‑1 (MQ=255)
                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                       >  2:4047540/1‑101 (MQ=9)
                                                                    aggggcgctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                  <  2:7527352/93‑1 (MQ=255)
                                                                    aggggcgctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                  <  1:8387093/93‑1 (MQ=255)
                                                                      gggcgctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa                                >  1:9061010/7‑101 (MQ=16)
                                                                         cgctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                             <  1:2787232/98‑1 (MQ=16)
                                                                           ctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                           <  1:1264021/100‑1 (MQ=255)
                                                                            tGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                          <  2:5614868/101‑1 (MQ=16)
                                                                             gCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                         <  1:616045/101‑1 (MQ=19)
                                                                               cccTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                       >  1:7420814/1‑101 (MQ=255)
                                                                                ccTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                      >  1:3785279/1‑101 (MQ=255)
                                                                                  tcctGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgc                    >  1:2575379/4‑101 (MQ=255)
                                                                                  tcctGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGCCCCGAAGGGCAAGAcgc                    >  1:360605/4‑101 (MQ=16)
                                                                                       gCCTTTC.GCC.CAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa               <  2:8089587/101‑1 (MQ=19)
                                                                                          tttC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg            >  2:5377927/1‑101 (MQ=255)
                                                                                             c.G.G.CCAATCCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc         <  2:9014228/101‑1 (MQ=19)
                                                                                               cggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc         <  2:4984176/97‑1 (MQ=25)
                                                                                                   cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc     >  2:5190560/1‑101 (MQ=255)
                                                                                                    aaTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTGGGCGCAAATCCGCCTCTGGCCCGAAGGCCAAGCCGCGCCGAAGGCGCGGccc    <  1:2019150/101‑1 (MQ=255)
                                                                                                      ttCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGATGGCGCGGccccc  <  1:8121503/101‑1 (MQ=25)
                                                                                                        |                                                                                                       
CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.G.G.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCC  >  NC_014034/1696082‑1696285

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG