Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 1,696,184 | C→A | 95.1% | intergenic (‑112/+113) | RCAP_rcc01564 ← / ← mmsA | isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase |
Read alignment evidence... | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 1,696,184 | 0 | C→A | 95.1% | 40.4 ‑1.1 ‑0.9 | 456 | intergenic (‑112/+113) | RCAP_rcc01564/mmsA | isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase |
Reads supporting (aligned to +/- strand): new base (220/208): ref base (11/16): total (231/225) | ||||||||||
Fisher's exact test strand distribution p-value = 3.24e-01 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.22e-01 |
Only 100 of 468 total aligned reads displayed.
AAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCC > NC_014034/1696085‑1696285
|
aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.cc > 1:3989713/1‑101 (MQ=255)
aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.ccc < 2:2536666/101‑1 (MQ=255)
gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.cccc < 1:4470687/101‑2 (MQ=37)
gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.CCCcgg > 2:759173/1‑101 (MQ=37)
gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.CCCcgg > 1:3249673/1‑101 (MQ=37)
gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCA.CCCcgg > 1:8575659/1‑101 (MQ=37)
ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCggg < 2:1735253/101‑1 (MQ=37)
ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCggg > 1:1660251/1‑101 (MQ=255)
cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGa > 1:4820394/1‑101 (MQ=255)
cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGa < 2:5858495/101‑1 (MQ=255)
cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGa < 2:2348854/101‑1 (MQ=255)
gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGatg > 2:241690/1‑99 (MQ=255)
aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGatgt < 1:1333073/101‑4 (MQ=255)
aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGatgtt < 2:2847999/101‑5 (MQ=255)
aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGatgtt > 1:7000019/1‑97 (MQ=25)
ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGatgttt < 1:1850359/101‑6 (MQ=25)
tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTg > 2:3765442/1‑101 (MQ=255)
tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTg > 1:3407393/1‑101 (MQ=255)
tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTg > 1:60860/1‑101 (MQ=255)
tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.ACCGGGATGTTTg > 2:8135342/1‑101 (MQ=255)
tGAACGTCTGTTTAATGTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTg < 2:1987588/101‑1 (MQ=255)
aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTggg > 2:4817701/1‑101 (MQ=255)
aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTggg > 1:5445828/1‑101 (MQ=255)
cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGcc < 1:5125584/101‑1 (MQ=255)
tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaa < 2:4175530/101‑1 (MQ=255)
cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaag < 1:23557/101‑2 (MQ=255)
cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaag < 2:4973319/101‑2 (MQ=255)
cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaag < 1:3154232/101‑2 (MQ=255)
cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaag > 1:3570014/1‑100 (MQ=21)
tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaagg < 1:4869644/101‑3 (MQ=19)
tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg > 1:5919440/1‑97 (MQ=19)
tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg < 2:6389652/101‑5 (MQ=255)
tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg > 1:937686/1‑97 (MQ=19)
ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga > 1:7179833/1‑96 (MQ=255)
tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa < 2:1178228/101‑7 (MQ=255)
aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaaa < 1:5807632/101‑8 (MQ=255)
aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAac < 1:6180845/101‑1 (MQ=16)
aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAac > 2:4623506/1‑101 (MQ=255)
tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAaca < 2:528911/101‑2 (MQ=255)
tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAaca > 1:2925836/1‑100 (MQ=255)
tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GCTT.CCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacc < 1:8962488/101‑4 (MQ=16)
cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGAGCCAA..GGTGAAacacct < 2:5626890/101‑5 (MQ=255)
gAATCCTTGCACGAATTTTGCAAGGAGGGGAGTGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt < 1:6253168/101‑6 (MQ=255)
gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt > 1:9120559/1‑96 (MQ=12)
gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt > 2:8271786/1‑96 (MQ=12)
gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt < 1:2775950/101‑6 (MQ=255)
gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt > 1:776384/1‑96 (MQ=12)
gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt < 2:4512623/101‑6 (MQ=255)
aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc > 1:8313772/1‑94 (MQ=255)
aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc < 1:2547182/101‑8 (MQ=255)
aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc > 2:7690978/1‑94 (MQ=255)
aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGCGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc < 1:4673592/101‑8 (MQ=255)
cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt < 2:6073741/101‑11 (MQ=255)
cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt > 1:3883826/1‑91 (MQ=255)
gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc < 2:3249574/101‑1 (MQ=255)
cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt < 1:1117682/101‑1 (MQ=255)
cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt < 1:7956894/101‑1 (MQ=255)
cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg > 2:1406863/1‑101 (MQ=255)
gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg > 1:367493/1‑101 (MQ=9)
gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg < 1:4395264/101‑1 (MQ=255)
aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GCTGAAA.CACCTT.GCTTTTTCTTGgc < 2:4371030/101‑1 (MQ=9)
tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa > 2:8637723/1‑101 (MQ=255)
tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa > 1:5535828/1‑101 (MQ=255)
ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa > 2:5131253/1‑101 (MQ=255)
ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa < 1:6827683/101‑1 (MQ=255)
tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa < 2:92221/101‑1 (MQ=255)
tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa > 2:1655709/1‑101 (MQ=9)
gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt < 1:4313734/101‑1 (MQ=9)
gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt > 1:6702561/1‑101 (MQ=255)
gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt > 1:5716164/1‑101 (MQ=255)
gCAAGGAGGGGAGAGGGGC..CCTGCCCCGCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt < 2:7048738/101‑1 (MQ=9)
cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa > 2:6671757/1‑101 (MQ=255)
cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa < 2:4739872/101‑1 (MQ=255)
aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg > 1:97208/1‑101 (MQ=255)
ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc > 2:3435811/1‑101 (MQ=255)
aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct > 2:7878682/1‑101 (MQ=12)
aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct < 1:1576521/101‑1 (MQ=9)
aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct < 2:6914665/101‑1 (MQ=9)
ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc > 1:7512077/1‑101 (MQ=12)
gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct < 2:8804757/101‑1 (MQ=255)
gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct > 2:4047540/1‑101 (MQ=9)
agaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc < 1:4623641/91‑1 (MQ=255)
agaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc < 2:5330525/91‑1 (MQ=255)
gggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa > 2:3527922/7‑101 (MQ=16)
gcgctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg < 1:8457017/97‑1 (MQ=255)
cgctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg < 1:4088655/98‑1 (MQ=16)
ctGCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc < 1:1264021/100‑1 (MQ=255)
gCCCCTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa > 2:221261/1‑101 (MQ=255)
cccTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc < 2:6181029/101‑1 (MQ=16)
cccTCCTGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc > 1:7420814/1‑101 (MQ=255)
tcctGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgc > 2:5395481/4‑101 (MQ=255)
tGGCCTTTCG.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc > 2:1844309/1‑101 (MQ=255)
gCCTTTCGCC.CAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa < 2:8089587/101‑1 (MQ=19)
cG.G.CCAATCCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc < 2:9014228/101‑1 (MQ=19)
ggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg > 1:2186895/4‑101 (MQ=255)
cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc > 2:2695705/1‑101 (MQ=255)
aaTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTGGGCGCAAATCCGCCTCTGGCCCGAAGGCCAAGCCGCGCCGAAGGCGCGGccc < 1:2019150/101‑1 (MQ=255)
aaTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc < 2:4540326/101‑1 (MQ=25)
aaTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGAGCAAGCCGCGCCGAAGGCCAGGccc > 2:7234286/1‑101 (MQ=19)
ttCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGATGGCGCGGccccc < 1:8121503/101‑1 (MQ=25)
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AAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCC > NC_014034/1696085‑1696285
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 23 ≤
ATCG
< 30 ≤
ATCG
< 34 ≤
ATCG
< 41 ≤
ATCG
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