BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,696,195	G→T	100%	intergenic (‑123/+102)	RCAP_rcc01564 ← / ← mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,696,195	0	G→T	100.0%	1405.1	432	intergenic (‑123/+102)	RCAP_rcc01564/mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand): new base (220/211): ref base (1/0): total (221/211)

Only 100 of 442 total aligned reads displayed.


TGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTG  >  NC_014034/1696097‑1696294


tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTg                                                                                                  >  2:3765442/1‑101 (MQ=255)


tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTg                                                                                                  >  1:3407393/1‑101 (MQ=255)


 gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTgg                                                                                                 <  2:4443020/101‑1 (MQ=255)


  aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTggg                                                                                                <  2:3042882/101‑1 (MQ=255)


  aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTggg                                                                                                <  1:7498158/101‑1 (MQ=25)


  aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTggg                                                                                                >  2:4817701/1‑101 (MQ=255)


   aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGc                                                                                               >  1:2335811/1‑101 (MQ=255)


   aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGc                                                                                               >  2:6916556/1‑101 (MQ=255)


    cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGcc                                                                                              <  1:3494553/101‑1 (MQ=255)


    cGTCTGTCTAATGTCGAATCCTTGCACGAATTGTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGCTGTTTGGGcc                                                                                              <  2:5929557/101‑1 (MQ=255)


       cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                                                           >  1:1614626/1‑100 (MQ=21)


       cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                                                           >  1:9142452/1‑100 (MQ=21)


       cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                                                           >  2:4185393/1‑100 (MQ=21)


       cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                                                           >  2:54869/1‑100 (MQ=21)


          tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                                                                        >  1:5919440/1‑97 (MQ=19)


          tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                                                                        <  1:8907874/101‑5 (MQ=255)


           ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                                                       >  2:5721779/1‑96 (MQ=255)


           ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                                                       >  2:241031/1‑96 (MQ=255)


            tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                                      >  2:9022230/1‑95 (MQ=255)


             aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                                     <  1:5807632/101‑8 (MQ=255)


               tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                 <  1:3918602/101‑2 (MQ=255)


               tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                 <  2:528911/101‑2 (MQ=255)


                ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                                <  2:1979051/101‑3 (MQ=255)


                ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                                >  2:3585672/1‑99 (MQ=12)


                 tccaaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                               <  1:902012/98‑4 (MQ=12)


                  cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                              <  1:6077631/101‑5 (MQ=255)


                   gAATCCTTGCACGAATTTTGCAAGGAGGGGAGTGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                             <  1:6253168/101‑6 (MQ=255)


                   gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                             <  1:6711735/101‑6 (MQ=255)


                    aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                            >  2:705191/1‑95 (MQ=255)


                     aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                           >  2:7690978/1‑94 (MQ=255)


                     aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGCGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                           <  1:4673592/101‑8 (MQ=255)


                      tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                          >  1:5214767/1‑93 (MQ=12)


                      tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                          >  1:7973157/1‑93 (MQ=12)


                       ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                         <  2:6684122/101‑10 (MQ=9)


                        cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                        <  2:2285414/101‑11 (MQ=255)


                        cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                        >  1:74018/1‑91 (MQ=255)


                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                   <  2:3249574/101‑1 (MQ=255)


                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                   <  1:3639127/101‑1 (MQ=255)


                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                   >  2:7904628/1‑101 (MQ=255)


                            cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                  <  1:1117682/101‑1 (MQ=255)


                              cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                                >  2:1406863/1‑101 (MQ=255)


                               gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                               >  1:4839558/1‑101 (MQ=9)


                               gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                               <  1:4395264/101‑1 (MQ=255)


                               gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                               <  2:617207/101‑1 (MQ=255)


                                gaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                              <  2:8738536/100‑1 (MQ=9)


                                aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCGCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                              <  1:927419/101‑1 (MQ=9)


                                  ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                            >  1:3252056/1‑101 (MQ=9)


                                    ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                          >  1:953557/1‑101 (MQ=255)


                                    ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                          <  1:6827683/101‑1 (MQ=255)


                                    ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                          >  2:5131253/1‑101 (MQ=255)


                                     tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                         <  2:92221/101‑1 (MQ=255)


                                     tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                         >  2:1655709/1‑101 (MQ=9)


                                      gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                        >  1:5488121/1‑101 (MQ=255)


                                      gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                        <  1:4301883/101‑1 (MQ=9)


                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                       <  2:4537870/101‑1 (MQ=255)


                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                       <  2:474087/101‑1 (MQ=255)


                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                       <  1:6982807/101‑1 (MQ=255)


                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                       <  2:1763280/101‑1 (MQ=255)


                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                       >  2:8145966/1‑101 (MQ=255)


                                        aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                      <  1:553996/101‑1 (MQ=255)


                                        aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                      >  1:7787257/1‑101 (MQ=9)


                                        aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                      <  1:1376034/101‑1 (MQ=255)


                                         aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACg                                                     <  2:8831328/101‑1 (MQ=9)


                                         aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                     >  1:2963891/1‑101 (MQ=255)


                                          ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                    >  2:2668377/1‑101 (MQ=255)


                                          ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                    >  2:2717133/1‑101 (MQ=255)


                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                  <  1:5721986/101‑1 (MQ=9)


                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                <  2:1142843/101‑1 (MQ=255)


                                                   agaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                                             <  1:4623641/91‑1 (MQ=255)


                                                       gggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa                                         >  1:8485982/7‑101 (MQ=16)


                                                          cgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                      <  1:4088655/98‑1 (MQ=16)


                                                           gctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                                     <  2:8519899/99‑1 (MQ=255)


                                                            ctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                                    <  1:1264021/100‑1 (MQ=255)


                                                              gCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                  >  2:3496701/1‑101 (MQ=19)


                                                              gCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                  >  2:221261/1‑101 (MQ=255)


                                                               ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCGAg                                 <  2:74018/101‑1 (MQ=255)


                                                                 ccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                               >  1:3785279/1‑101 (MQ=255)


                                                                  cTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg                              <  1:2683013/101‑1 (MQ=255)


                                                                      tGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                          >  2:3351854/1‑101 (MQ=255)


                                                                       ggCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                         >  1:6163352/1‑101 (MQ=255)


                                                                       ggCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                         >  1:8336171/1‑101 (MQ=255)


                                                                        gCCTTTCGCC.CAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa                        <  2:8089587/101‑1 (MQ=19)


                                                                         ccTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                       <  2:5333981/101‑1 (MQ=19)


                                                                               cggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc                  <  2:4984176/97‑1 (MQ=25)


                                                                                 gccAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCgg                <  1:7055821/99‑1 (MQ=25)


                                                                                 gccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCgg                >  1:2136670/3‑101 (MQ=255)


                                                                                 gccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCgg                >  2:4553415/3‑101 (MQ=255)


                                                                                  ccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGc               >  1:4976106/2‑101 (MQ=255)


                                                                                   cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc              >  1:4490264/1‑101 (MQ=255)


                                                                                    aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTGGGCGCAAATCCGCCTCTGGCCCGAAGGCCAAGCCGCGCCGAAGGCGCGGccc             <  1:2019150/101‑1 (MQ=255)


                                                                                    aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc             >  2:4717663/1‑101 (MQ=255)


                                                                                    aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGAGCAAGCCGCGCCGAAGGCCAGGccc             >  2:7234286/1‑101 (MQ=19)


                                                                                     attcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc            >  2:6539797/7‑101 (MQ=255)


                                                                                     aTTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc            <  1:8146315/101‑1 (MQ=25)


                                                                                      ttcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc           >  2:1841793/6‑101 (MQ=255)


                                                                                        caccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt         >  1:2809217/4‑101 (MQ=255)


                                                                                         cccccGGGATGTTGGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg        <  1:7660130/99‑1 (MQ=255)


                                                                                          ccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGa       <  1:2520662/100‑1 (MQ=37)


                                                                                           cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt      <  1:1792954/101‑1 (MQ=37)


                                                                                             cGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCg    >  2:387426/1‑101 (MQ=255)


TGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTG  >  NC_014034/1696097‑1696294

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG