Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,732,849 C→G 10.9% intergenic (‑273/‑130) RCAP_rcc01601 ← / → tgt FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,732,8490C→G10.9% 8.2 ‑3.3 0.0119intergenic (‑273/‑130)RCAP_rcc01601/tgtFeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/15):  ref base (25/79):  total (25/94)
Fisher's exact test strand distribution p-value = 3.85e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

AGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC....GT...C...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCG  >  NC_014034/1732749‑1732946
                                                                                                    |                                                                                                                 
aGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGTCCCCGCTTTTCCTAGGCAAAATTCGCCCTTCTTTCTTTTCGGGGGGGc                                                                                                                   <  1:7478921/101‑1 (MQ=255)
aGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGc                                                                                                                   >  2:1434184/1‑101 (MQ=255)
aGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGc                                                                                                                   >  1:2704042/1‑101 (MQ=255)
  cGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCgg                                                                                                                 >  2:4139111/1‑101 (MQ=255)
   gggACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCggg                                                                                                                >  2:6382522/1‑101 (MQ=255)
    ggACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCgggg                                                                                                               <  2:3103910/101‑1 (MQ=255)
    ggACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCgggg                                                                                                               >  2:6927303/1‑101 (MQ=255)
     gcccAGGCCACGCTGCAGGCGCAGGCCGGCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCggggg                                                                                                              <  2:7347126/99‑1 (MQ=255)
     gACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCggggg                                                                                                              >  2:400595/1‑101 (MQ=255)
     gACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCggggg                                                                                                              <  1:4200564/101‑1 (MQ=255)
     gACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCggggg                                                                                                              >  1:2176688/1‑101 (MQ=255)
     gACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCggggg                                                                                                              <  1:3129194/101‑1 (MQ=255)
       ccAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCggggggg                                                                                                            >  2:3681569/1‑100 (MQ=255)
       ccAGCCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGt                                                                                                            <  2:338694/101‑1 (MQ=255)
        cAGGCCCCCCTGCGGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTg                                                                                                           <  2:675286/101‑1 (MQ=255)
         aGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCggggggggg                                                                                                          >  1:6786762/1‑98 (MQ=255)
         aGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCggggggggg                                                                                                          >  2:623921/1‑98 (MQ=255)
         aGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCggggggggg                                                                                                          >  1:4836489/1‑98 (MQ=255)
         aGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGa                                                                                                          <  1:2548077/101‑1 (MQ=255)
         aGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGa                                                                                                          <  1:1877050/101‑1 (MQ=255)
         aGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGa                                                                                                          <  2:7318196/101‑1 (MQ=255)
         aGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGa                                                                                                          >  2:1067942/1‑101 (MQ=255)
           gCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCgggggggggag                                                                                                        >  1:7003895/1‑96 (MQ=255)
           gCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAg                                                                                                        <  1:5149458/101‑1 (MQ=255)
            ccccccTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...c                                                                                                    <  1:8499829/101‑1 (MQ=255)
             cccccTGCAGCCGCATGCCGTCCGCGTCAACAGGCAATGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...cc                                                                                                   <  1:9006183/101‑1 (MQ=255)
             cccccTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAG...cc                                                                                                   >  1:5251893/1‑101 (MQ=255)
              cggcTGCAGCCGGATGCCGCGCGGGTGAGCCGGCAAGGGCCCCGCTTTTCCTAGGTAAAATGCGCCCTTCTTTCTTATCGGGGGGGGGGGGGGTGAGG...ccc                                                                                                  <  2:9024881/98‑1 (MQ=255)
              ccccTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...ccc                                                                                                  <  1:8596676/101‑1 (MQ=255)
              ccccTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...ccc                                                                                                  <  1:6851363/101‑1 (MQ=255)
               cccTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCggggggggggggccc                                                                                                    >  2:383720/1‑92 (MQ=255)
               cccTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...cccc                                                                                                 <  1:7396425/101‑1 (MQ=255)
               cccTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...cccc                                                                                                 >  2:2347479/1‑101 (MQ=255)
               cccTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAG...gccc                                                                                                 >  2:5364835/1‑101 (MQ=255)
                ccTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...ccccc                                                                                                <  2:2456263/101‑1 (MQ=255)
                 cTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...cccccc                                                                                               <  1:2406561/101‑1 (MQ=255)
                 cTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...cccccc                                                                                               <  2:3895712/101‑1 (MQ=255)
                    cAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC....gcg                                                                                        <  1:1814054/101‑3 (MQ=255)
                    cAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAG...ccccccccc                                                                                            >  1:2617009/1‑98 (MQ=255)
                    cAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGAAG...caccccccc                                                                                            >  2:5632416/1‑98 (MQ=255)
                     aGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC....gcgg                                                                                       >  2:3766251/1‑98 (MQ=255)
                        cGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC....gcgggtc                                                                                    <  1:5551296/101‑7 (MQ=255)
                         gCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAG...gccccccccgggtg                                                                                       >  2:8799210/1‑93 (MQ=255)
                         gCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAG...gccccccccgggcg                                                                                       >  2:5164857/1‑93 (MQ=255)
                         gCATCCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC....gcgggtcg                                                                                   <  1:1064219/101‑8 (MQ=255)
                          cATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAG...gcccccccggggcgc                                                                                      >  1:3805046/1‑92 (MQ=255)
                           aTGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAG...ccccccccggggcggc                                                                                     >  2:6426841/1‑91 (MQ=255)
                                          acgggCAGGGCCCCCGCTTTTCCTGGGCAAAATACGCCCTCCTTTCTTTTCGGGGGGGCGGGGGTTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAg                                                              <  2:571090/98‑1 (MQ=12)
                                             ggCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.g                                                          <  1:7822077/101‑1 (MQ=21)
                                             ggCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC....GCGGGT...CG.CCTCGGGCTTGCCCGAGGC.g                                                          <  2:7740725/101‑1 (MQ=21)
                                             gccagggcccccGTTTTCCCTGGGCAAAATCCCCCCTTCTTTCTTTGGGGGGGGGGGGGGGGGAAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.g                                                          <  2:4614877/93‑1 (MQ=2)
                                              gCAAGGGCCCCGCTTTTCCTAGGCAAACTACGCCCTTCTTTCTTTGCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.Ga                                                         <  2:929356/101‑1 (MQ=18)
                                              gCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.Ga                                                         <  1:921973/101‑1 (MQ=21)
                                               cAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.Gaa                                                        >  2:3870616/1‑101 (MQ=21)
                                                 aGGGCCGCGCTGTCCCTAGTCAAACTTCGCCCTTCTTTCTTTGCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAg                                                      <  2:2887872/101‑1 (MQ=9)
                                                  gggCCCGGTTTTTCCGGGGAAAAGTCCGCCCTTGTTTTTGTGGGGGGGGGGGGGGGGGGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGc                                                     <  1:6290766/101‑1 (MQ=2)
                                                   gcccccGTTTTTCCTAGGCAAAATCCCCCCTTTTTTCTTTGGGGGGGGGCGGGGGGGGAAC...CCCCCC....GCGGGT...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCt                                                    <  1:67034/99‑1 (MQ=2)
                                                     cacggCTTTTCCGGGCCAAAATGGGCTTTTGTTTCTTGCAGGGGGGGGAGAGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.c                                                 <  1:1283412/97‑1 (MQ=2)
                                                      cccGCTTTTCCTAGGCAAAATCCGCCCTTCTTTCTTGTCGGGGGGGGGGGGGGTGAAC...CCCCCC....GCGGGT...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cc                                                <  1:8173736/101‑1 (MQ=18)
                                                       cgtcTTTTCCTAGGCAAAATCCCCCTTTCTTTCTTTGGGGGGGGGCGGGGGGGGAAG...CCCCCC....GCGGGT...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.ccc                                               <  2:55053/98‑1 (MQ=2)
                                                       ccGCTTTCCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC....GC...GGGTCG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.ccc                                               <  2:8669873/101‑1 (MQ=18)
                                                        cGCTTTTCCTGGGCAAAATGCGCCCTTCTTTCTTTGCGGGGGGGCGGGGGGTGAAG...CCCCCC....GC...GGGTCG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cccc                                              <  2:2508570/101‑1 (MQ=17)
                                                            tttCCTAGGCAAAATCCGCCCTTCTTTCTTTGGGGGGGGGGGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGt                                          <  1:4570822/101‑1 (MQ=17)
                                                              tCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAGGC.CCCCCCG...GG...G...CGCCCCCGGGCTGGCCCCCGGC.GGAAGCTT.ccccccccc                                         >  1:6426769/1‑98 (MQ=255)
                                                              tCCTAGGAAAAATCCCCCTTTTTTTTTTTGGGGGGGGGGGGGGGGTGACC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGa                                        <  1:3310579/101‑1 (MQ=2)
                                                               ccTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGaa                                       <  2:2580140/101‑1 (MQ=21)
                                                                  agtcAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAc                                    <  2:9143811/98‑1 (MQ=18)
                                                                  aGGCAAATTCCCCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAc                                    <  1:1687178/101‑1 (MQ=17)
                                                                  aGGCAAAATACGCTCTTCTTTCTTTTGGGGGGGGCGGGGGTTGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAc                                    <  2:3960076/101‑1 (MQ=18)
                                                                  aGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAc                                    <  2:8439840/101‑1 (MQ=21)
                                                                    gCAAGATGCCCCCTTCTTTTGTTAGGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCt                                  <  2:7089179/101‑1 (MQ=9)
                                                                    gCAAAAGACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGTGGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCt                                  <  1:7302494/101‑1 (MQ=18)
                                                                     cAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCtt                                 <  2:5169475/101‑1 (MQ=21)
                                                                     cAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGAAG...CCCCCCGCG.GG...G...CG.CCTCGGGGCTGGCCGAGGG.GGAAGCTT.CCCCCCCGGGAAAccct                                 >  1:2980902/1‑99 (MQ=12)
                                                                       aaaTACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGGAGGC.CCCCCCGG..GG...C...GG.CCCCCGCCTTCCCCGGGGG.GAAAGCGT.CCCCCCCGGAAAAccctc                                >  1:6050026/1‑98 (MQ=255)
                                                                        aattccgccTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGcc                              <  1:5888929/94‑1 (MQ=18)
                                                                        aatgcccccTTCTTTCTTTGCGGGGGGGCGGGGGGTGCAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGcc                              <  1:5402615/95‑1 (MQ=17)
                                                                             gtccTTCTTTCTTTTGGGGGGGGGGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTgcgc                         <  2:8334277/99‑1 (MQ=18)
                                                                                  tctttcttTTCGGGGGGGCGGGGGGGGAAG...CCCCCCCCGGGG...C...CG.CCCCGGGCTTGCCCCGGGG.GAAACCTT.CCCCCC.CGAAAAACCTGCCCgcgccccgc                    >  1:8914027/1‑96 (MQ=255)
                                                                                  tctttcttTTCGGGGGGGCGGGGGGGGAAG...CCCCCCCCG.GG...G...GG.CCCCGGGGTTGCCCCCGGC.GGAAGCTTCCCCCCCGGGAAAACCTTGccgccccgccc                     >  2:907546/1‑92 (MQ=255)
                                                                                   tttccttgTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCg                   <  2:9003824/97‑1 (MQ=18)
                                                                                   ctgtctttGCGGGGGGGCGGGGGTTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCg                   <  2:4791002/98‑1 (MQ=18)
                                                                                     ttctttTCGGGGGGGCGGGGGGGGGAGGCCCCCCCCG...GG...C...GG.CCCCGGGGTGGCCCCGGGG.GAACGCTC.CCCCCCCGGAAAACCTT.CCTTcgcgcggcccc                 >  1:6013366/1‑96 (MQ=255)
                                                                                     ttctttTCGGGGGGGCGGGGGGGGAAG...CCCCCCGCG.GG...G...CG.CCTCGGGCGTGCCCCCGGG.GGCAGGTT.CCCCCCCTGAAAAACCTGCCTGCGCGCGGCGcc                 >  1:4386855/1‑100 (MQ=255)
                                                                                      tctttTCGGGGGGGCGGGGGGGGGAGGC.CCCCCCGG..GG...C...GG.CCCGGGGGTGGCCCGGGGG.GCAAGCTT.CCCCCCCCGAAAACCTGCCCTGCGCGCGGCGCt                 >  1:1162582/1‑101 (MQ=255)
                                                                                       ttttgggggggggGGGGGGGGGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt               <  1:383728/95‑1 (MQ=17)
                                                                                       ctttTGGGGGGGGGGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt               <  2:4139532/101‑1 (MQ=18)
                                                                                       ctttTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt               <  2:2084553/101‑1 (MQ=21)
                                                                                       ctttTCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt               <  1:4139230/101‑1 (MQ=21)
                                                                                        tttgCGGGGGGGGGGGGGGTGACG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTc              <  1:9181550/101‑1 (MQ=18)
                                                                                         tttCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTcc             <  1:56048/101‑1 (MQ=21)
                                                                                         ttgggggggggGGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTcc             <  1:4961944/97‑1 (MQ=18)
                                                                                          ttCGGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTccc            <  2:3511610/101‑1 (MQ=21)
                                                                                          ttCGGGGGGGCGGGGGGGGAAG...CCCCCCCCG.GG...G...CG.CCCCCGGCTTGCCCCCGGCGGAAACATT.CCCCCCCGGAAACACTT.CCTGGGCGCGGCGCTTTcgc            >  2:5968239/1‑99 (MQ=255)
                                                                                               gggggCGGGGGGTGACG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCg       <  2:5301255/101‑1 (MQ=21)
                                                                                                 gggCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGc     <  1:5688135/101‑1 (MQ=21)
                                                                                                 gggCGGGGGGGGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGc     <  2:8008591/101‑1 (MQ=21)
                                                                                                  ggCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGcc    <  1:331132/101‑1 (MQ=21)
                                                                                                    cGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg  >  2:2430284/1‑101 (MQ=21)
                                                                                                    cGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg  >  2:267526/1‑101 (MQ=21)
                                                                                                    |                                                                                                                 
AGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG...CCCCCC....GT...C...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCG  >  NC_014034/1732749‑1732946

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG