Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,732,868 T→C 8.5% intergenic (‑292/‑111) RCAP_rcc01601 ← / → tgt FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,732,8680G→C 8.5% 10.6 ‑0.5 ‑0.582intergenic (‑292/‑111)RCAP_rcc01601/tgtFeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/7):  ref base (10/65):  total (10/72)
Fisher's exact test strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.95e-01

CGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCC....GT...C...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGGC  >  NC_014034/1732780‑1732948
                                                                                                |                                                                                         
cGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGGGAAG...CCCCCC....GC...C...GG.ccgcccccg                                                                        >  1:8193809/1‑94 (MQ=255)
        gcAACAGGAGAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCC....GCGGGT...CG.CCTCGGGCTTGccc                                                                   <  2:6776328/100‑1 (MQ=21)
           acgggCAGGGCCCCCGCTTTTCCTGGGCAAAATACGCCCTCCTTTCTTTTC.GGGGGGGCGGGGGTTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAg                                                                <  2:571090/98‑1 (MQ=12)
            cAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATATGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAgg                                                               <  2:934971/101‑1 (MQ=21)
             gggCAAGGGCCCCGCTTTTCCGAGGCAAAATCCCCCCTTTTTTTTTTTG.GGGGGGGCGGGGGGTGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGc                                                              <  1:2347546/100‑1 (MQ=9)
              ggCAGGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTTTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.g                                                            <  1:4492154/101‑1 (MQ=18)
              ggCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.g                                                            <  1:7822077/101‑1 (MQ=21)
              ggCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.g                                                            <  2:8963130/101‑1 (MQ=21)
              ggCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCC....GCGGGT...CG.CCTCGGGCTTGCCCGAGGC.g                                                            <  2:7740725/101‑1 (MQ=21)
              gccagggcccccGTTTTCCCTGGGCAAAATCCCCCCTTCTTTCTTTGG.GGGGGGGGGGGGGGGAAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.g                                                            <  2:4614877/93‑1 (MQ=2)
               gCAAGGGCCCCGCTTTTCCTAGGCAAACTACGCCCTTCTTTCTTTGC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.Ga                                                           <  2:929356/101‑1 (MQ=18)
               gCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.Ga                                                           <  1:921973/101‑1 (MQ=21)
                cAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.Gaa                                                          >  2:3870616/1‑101 (MQ=21)
                  aGGGCCGCGCTGTCCCTAGTCAAACTTCGCCCTTCTTTCTTTGC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAg                                                        <  2:2887872/101‑1 (MQ=9)
                   gggCCCGGTTTTTCCGGGGAAAAGTCCGCCCTTGTTTTTGTGG.GGGGGGGGGGGGGGGGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGc                                                       <  1:6290766/101‑1 (MQ=2)
                    gcccccGTTTTTCCTAGGCAAAATCCCCCCTTTTTTCTTTGG.GGGGGGGCGGGGGGGGAAC...CCCCCC....GCGGGT...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCt                                                      <  1:67034/99‑1 (MQ=2)
                      cacggCTTTTCCGGGCCAAAATGGGCTTTTGTTTCTTGCA.GGGGGGGGAGAGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.c                                                   <  1:1283412/97‑1 (MQ=2)
                       cccGCTTTTCCTAGGCAAAATCCGCCCTTCTTTCTTGTC.GGGGGGGGGGGGGGTGAAC...CCCCCC....GCGGGT...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cc                                                  <  1:8173736/101‑1 (MQ=18)
                        cgtcTTTTCCTAGGCAAAATCCCCCTTTCTTTCTTTGG.GGGGGGGCGGGGGGGGAAG...CCCCCC....GCGGGT...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.ccc                                                 <  2:55053/98‑1 (MQ=2)
                        ccGCTTTCCCTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCC....GC...GGGTCG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.ccc                                                 <  2:8669873/101‑1 (MQ=18)
                         cGCTTTTCCTGGGCAAAATGCGCCCTTCTTTCTTTGC.GGGGGGGCGGGGGGTGAAG...CCCCCC....GC...GGGTCG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cccc                                                <  2:2508570/101‑1 (MQ=17)
                           cTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTG.GGGGGGGGGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cccccc                                              <  1:5297489/101‑1 (MQ=18)
                           cTTTCCCTAGGCAAAATCCCCCCTTTTTTCTTTTG.GGGGGGGCGGGGGGTGACG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.cccccc                                              <  1:5963820/101‑1 (MQ=9)
                            ttttCCTAGGCACAATTCGCCTTTCTTTCTTTGC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCg                                             <  2:4879547/101‑1 (MQ=17)
                            ttttCCTAGGCAAAATCCGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCg                                             <  2:4676943/101‑1 (MQ=18)
                             tttCCTAGGCAAAATCCGCCCTTCTTTCTTTGG.GGGGGGGGGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGt                                            <  1:4570822/101‑1 (MQ=17)
                              ttCCTAGGCAAAATGCGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTg                                           <  2:4103966/101‑1 (MQ=18)
                              ttCCTAGGCAAAANCCGCCTNNNTTTNTTNNC.GGGGGGGCGGGGGTTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTg                                           <  1:3639305/101‑1 (MQ=17)
                              tcccTAGGCAAAATCCGCCCTTCTTTCTTTTG.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTg                                           <  2:4534751/99‑1 (MQ=17)
                               tCCTAGGCAAAATCCGCCCTTTTTTCTTTTG.GGGGGGGGGGGGGGTGAAC...CCCCCCGCG.GG...T...CG.CCTTGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGa                                          <  1:4754998/101‑1 (MQ=12)
                               tCCTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGGGGAGGC.CCCCCCG...GG...G...CGCCCCCGGGCTGGCCCCCGGC.GGAAGCTT.ccccccccc                                           >  1:6426769/1‑98 (MQ=255)
                               tCCTAGGAAAAATCCCCCTTTTTTTTTTTGG.GGGGGGGGGGGGGGTGACC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGa                                          <  1:3310579/101‑1 (MQ=2)
                                ccTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGaa                                         <  2:2580140/101‑1 (MQ=21)
                                 cTAGGCAAAATCCGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGaaa                                        <  2:7003610/101‑1 (MQ=18)
                                   agtcAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAc                                      <  2:9143811/98‑1 (MQ=18)
                                   aGGCAAATTCCCCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGGGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAc                                      <  1:1687178/101‑1 (MQ=17)
                                   aGGCAAAATACGCTCTTCTTTCTTTTG.GGGGGGGCGGGGGTTGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAc                                      <  2:3960076/101‑1 (MQ=18)
                                   aGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAc                                      <  2:8439840/101‑1 (MQ=21)
                                    gacAAAAGTCGCCCTTCTTTCTTTGG.GGGGGGGCGGGGGTGGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAcc                                     <  1:5693992/99‑1 (MQ=9)
                                     gCAAGATGCCCCCTTCTTTTGTTAG.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCt                                    <  2:7089179/101‑1 (MQ=9)
                                     gCAAAAGACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGTGGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCt                                    <  1:7302494/101‑1 (MQ=18)
                                     gCAAAAAGCCCCCTTTTTTTTTTTCGGGGGGGGGGGGGGGTGACC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAAcc                                     <  2:3051691/101‑1 (MQ=9)
                                      cAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCtt                                   <  2:5169475/101‑1 (MQ=21)
                                      cAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGGGAAG...CCCCCCGCG.GG...G...CG.CCTCGGGGCTGGCCGAGGG.GGAAGCTT.CCCCCCCGGGAAAccct                                   >  1:2980902/1‑99 (MQ=12)
                                        aaaTACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGGGGAGGC.CCCCCCGG..GG...C...GG.CCCCCGCCTTCCCCGGGGG.GAAAGCGT.CCCCCCCGGAAAAccctc                                  >  1:6050026/1‑98 (MQ=255)
                                         aattccgccTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGcc                                <  1:5888929/94‑1 (MQ=18)
                                         aatgcccccTTCTTTCTTTGC.GGGGGGGCGGGGGGTGCAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGcc                                <  1:5402615/95‑1 (MQ=17)
                                         aaTCCGCCCTTCTTTCTTTGC.GGGGGGGGGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGcc                                <  1:2629042/101‑1 (MQ=18)
                                         aaTACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGcc                                <  1:7332099/101‑1 (MQ=21)
                                            aCGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGGGGAGCCCCCCCCC....GG...G...CGCCCCCGGGCTTCCCCGGGGG.GAAGGCTT.CCCCCCGGGAAAACCtgccccc                              >  2:5253443/1‑95 (MQ=255)
                                              gtccTTCTTTCTTTTG.GGGGGGGGGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTgcgc                           <  2:8334277/99‑1 (MQ=18)
                                                ccTTCTTTCTTGGG.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTgcgcgc                         <  1:9073136/101‑1 (MQ=17)
                                                   tctttcttTTC.GGGGGGGCGGGGGGGGAAG...CCCCCCCCGGGG...C...CG.CCCCGGGCTTGCCCCGGGG.GAAACCTT.CCCCCC.CGAAAAACCTGCCCgcgccccgc                      >  1:8914027/1‑96 (MQ=255)
                                                   tctttcttTTC.GGGGGGGCGGGGGGGGAAG...CCCCCCCCG.GG...G...GG.CCCCGGGGTTGCCCCCGGC.GGAAGCTTCCCCCCCGGGAAAACCTTGccgccccgccc                       >  2:907546/1‑92 (MQ=255)
                                                    tttccttgTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCg                     <  2:9003824/97‑1 (MQ=18)
                                                    ctgtctttGC.GGGGGGGCGGGGGTTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCg                     <  2:4791002/98‑1 (MQ=18)
                                                      ttctttTC.GGGGGGGCGGGGGGGGGAGGCCCCCCCCG...GG...C...GG.CCCCGGGGTGGCCCCGGGG.GAACGCTC.CCCCCCCGGAAAACCTT.CCTTcgcgcggcccc                   >  1:6013366/1‑96 (MQ=255)
                                                      ttctttTC.GGGGGGGCGGGGGGGGAAG...CCCCCCGCG.GG...G...CG.CCTCGGGCGTGCCCCCGGG.GGCAGGTT.CCCCCCCTGAAAAACCTGCCTGCGCGCGGCGcc                   >  1:4386855/1‑100 (MQ=255)
                                                      ttccttgtggggggggGGGCGGGTGAAG...CCCCCCGCG.GG...G...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGc                    <  1:922659/92‑1 (MQ=18)
                                                       tctttTC.GGGGGGGCGGGGGGGGGAGGC.CCCCCCGG..GG...C...GG.CCCGGGGGTGGCCCGGGGG.GCAAGCTT.CCCCCCCCGAAAACCTGCCCTGCGCGCGGCGCt                   >  1:1162582/1‑101 (MQ=255)
                                                        tttttgggggggggGGGGGGGTGAGC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCtt                  <  1:2135367/94‑1 (MQ=17)
                                                        ctttTG.GGGGGGGGGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                 <  2:4139532/101‑1 (MQ=18)
                                                        ctttTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                 <  1:5165026/101‑1 (MQ=21)
                                                        ctttTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                 <  1:4139230/101‑1 (MQ=21)
                                                        ctttTC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                 <  2:2084553/101‑1 (MQ=21)
                                                        ctttTC.GGGGGGGCGGGGGGGGAAGGC.CCCCCCGG..GG...G...GG.CCCCGGGGTGGCCCGGGGG.GAAGGGTC.CCCCCCGCGAAAACCTTTCC.GCGCGCGCCggctt                 >  2:6227605/1‑97 (MQ=255)
                                                         ttttgggggggggGGGGGGGGGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCttt                 <  1:383728/95‑1 (MQ=17)
                                                         tttgC.GGGGGGGGGGGGGGTGACG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTc                <  1:9181550/101‑1 (MQ=18)
                                                         tttcC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTc                <  2:3750071/101‑1 (MQ=18)
                                                          tttC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTcc               <  1:56048/101‑1 (MQ=21)
                                                          tttC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...G...CG.GCTCGGGGTTGCCCGGGGG.GAAAGCGT.TCCCCCGGGAAAAACTTTGCTGCGCGCGTGGGTTTcc               >  1:1338346/1‑101 (MQ=255)
                                                          tttC.GGGGGGGCGGGGGGCGAAG...CCCCCCGCG.GG...G...CG.CCTCCGGGTTGCCCGAGGC.GGAAGCTT.CCCCCCCCGAAAAACCTGCCTGCGCGCGGCCCCTTcc               >  2:6345974/1‑101 (MQ=2)
                                                           ttgggggggggGGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTcc               <  1:4961944/97‑1 (MQ=18)
                                                           ttC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTccc              <  2:796891/101‑1 (MQ=21)
                                                           ttC.GGGGGGGCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTccc              <  2:3511610/101‑1 (MQ=21)
                                                           ttC.GGGGGGGCGGGGGGGGAAG...CCCCCCCCG.GG...G...CG.CCCCCGGCTTGCCCCCGGCGGAAACATT.CCCCCCCGGAAACACTT.CCTGGGCGCGGCGCTTTcgc              >  2:5968239/1‑99 (MQ=255)
                                                             tggggggggGGGGGGGGGAGG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCt             <  1:322251/99‑1 (MQ=18)
                                                             c.GGGGGGGCGTGGGTGGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCtt            <  2:2246000/101‑1 (MQ=18)
                                                             c.GGGGGGGCGGGGGGGGAAG...CCCCCCGCG.GG...G...CG.CCCCCGGCGTGCCCGCGGCGGAAACGTC.CCCCCCCCGAAAACC.TGCCCCCGCGCGGCGCTTTCCCtt            >  2:8016072/1‑101 (MQ=255)
                                                                ggggggCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTc          <  2:8516181/101‑1 (MQ=21)
                                                                ggggggCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTc          <  2:1136105/101‑1 (MQ=21)
                                                                 gggggCGGGGGGTGACG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCg         <  2:5301255/101‑1 (MQ=21)
                                                                   gggGGGGGGGTGAAC...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGc       <  1:6303815/101‑1 (MQ=21)
                                                                   gggCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGc       <  1:5688135/101‑1 (MQ=21)
                                                                   gggCGGGGGGGGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGc       <  2:8008591/101‑1 (MQ=21)
                                                                    ggCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGcc      <  2:879266/101‑1 (MQ=21)
                                                                    ggCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGcc      <  1:331132/101‑1 (MQ=21)
                                                                     gCGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGccc     <  2:3950001/101‑1 (MQ=21)
                                                                      cGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg    >  1:3962372/1‑101 (MQ=21)
                                                                      cGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg    <  2:1699815/101‑1 (MQ=21)
                                                                      cGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg    >  2:2430284/1‑101 (MQ=21)
                                                                      cGGGGGGTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg    >  2:267526/1‑101 (MQ=21)
                                                                       ggggggTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGccccg   >  2:5569540/1‑99 (MQ=21)
                                                                       ggggggTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCg  >  1:7707072/1‑100 (MQ=21)
                                                                       ggggggTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGCCGCTTTCCCTTTCGGCCCgg   >  2:705857/1‑101 (MQ=21)
                                                                        gggggTGAAG...CCCCCCGCG.GG...T...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGgc  <  2:2717010/101‑1 (MQ=21)
                                                                                                |                                                                                         
CGTCCGCGTCAACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATACGCCCTTCTTTCTTTTC.GGGGGGGCGGGGGGTGAAG...CCCCCC....GT...C...CG.CCTCGGGCTTGCCCGAGGC.GAAAGCTT.CCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCTTTCGGCCCGGC  >  NC_014034/1732780‑1732948

Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 20 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG