Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,889 T→G 2.5% intergenic (‑142/+51) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8890T→G 2.5% 15.8 ‑4.2 ‑0.0477intergenic (‑142/+51)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/2):  ref base (207/257):  total (218/259)
Fisher's exact test strand distribution p-value = 1.50e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.95e-01

Only 100 of 503 total aligned reads displayed.

CAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGC  >  NC_014034/1734790‑1734989
                                                                                                     |                                                                                                      
 aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                       <  1:6522569/101‑1 (MQ=255)
 aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                       >  2:2899565/1‑101 (MQ=255)
      aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                                                                                 >  1:6066780/1‑101 (MQ=255)
         ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                              <  1:343646/101‑1 (MQ=255)
           cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                            >  2:2305913/1‑101 (MQ=255)
            tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATc                                                                                           >  1:5183745/1‑101 (MQ=255)
             gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                          <  1:4192734/101‑1 (MQ=255)
              gcgctcgggcAGCCGAGGGGCCGGGCAGGGGTGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCGTTCATCtt                                                                                         <  2:319987/92‑1 (MQ=14)
              aCGCTGCGTCAGCCGGCGGGCCGGGCATGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                         <  1:1613384/101‑1 (MQ=18)
              aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                         <  1:447756/101‑1 (MQ=255)
              aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                         <  2:8994823/101‑1 (MQ=255)
               cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                        <  2:5261444/101‑1 (MQ=255)
                  tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCa                                                                                     <  2:2172089/101‑1 (MQ=255)
                   gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGTGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                    <  2:4451659/101‑1 (MQ=255)
                   gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                    <  2:5561451/101‑1 (MQ=255)
                   gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                    <  1:6713945/101‑1 (MQ=255)
                   gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                    >  2:5713616/1‑101 (MQ=255)
                    ggTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                   <  2:611180/100‑1 (MQ=255)
                    cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                   <  1:1213689/101‑1 (MQ=255)
                     gTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                  <  2:111088/101‑1 (MQ=255)
                      tCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                 <  1:3708953/101‑1 (MQ=255)
                      tCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                 >  1:2456467/1‑101 (MQ=255)
                        aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGCTATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCAATAAata                                                                               >  1:4278739/1‑101 (MQ=17)
                         gCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                                                                              >  1:3320149/1‑101 (MQ=255)
                         gCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                                                                              <  1:9006888/101‑1 (MQ=255)
                         gCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                                                                              <  1:5361354/101‑1 (MQ=255)
                           cGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                            >  2:42606/1‑101 (MQ=255)
                           cGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                            <  2:6828081/101‑1 (MQ=255)
                               gggccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                                                                       <  1:2899663/101‑1 (MQ=255)
                                 tgacggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                    >  2:2469975/9‑101 (MQ=2)
                                 tgacggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                    >  1:3238446/9‑101 (MQ=2)
                                   cgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                   <  2:75811/101‑1 (MQ=255)
                                   cgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                   <  2:6360949/101‑1 (MQ=255)
                                    gggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                  <  2:5360201/101‑1 (MQ=255)
                                     ggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgg                                                                 <  1:7154886/101‑1 (MQ=255)
                                     ggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgg                                                                 >  2:2625040/1‑101 (MQ=255)
                                      gcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg                                                                <  1:5781132/101‑1 (MQ=255)
                                        tggggggCGCT.GCCCCCCGTGCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                              <  1:7254596/100‑1 (MQ=255)
                                        aGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGCTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGCGGc                                                              >  2:8535125/1‑101 (MQ=17)
                                        aGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                              <  2:1260499/101‑1 (MQ=255)
                                        aGGCGGGCGCTCC.CCCCCCTCCTGCGGACACCCCCCGCGATATTTATGGCAAGATGAAACCGC.CGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                              <  2:2832914/101‑1 (MQ=11)
                                           ggggCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCaga                                                           >  2:7742896/1‑101 (MQ=255)
                                             ggCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                         <  2:4044639/101‑1 (MQ=255)
                                               cgcT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGccc                                                       >  1:625133/1‑101 (MQ=255)
                                                 gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                      >  2:347622/6‑101 (MQ=255)
                                                  ctgccccccGTCCTGCGGACTCCCCCCGGGATATTTAAGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                     <  1:3776065/97‑1 (MQ=255)
                                                    gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                   <  2:3638613/99‑1 (MQ=255)
                                                    gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                   >  2:1976426/3‑101 (MQ=255)
                                                     ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                  <  2:3788042/100‑1 (MQ=255)
                                                     ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                  <  1:2987269/100‑1 (MQ=255)
                                                      cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                 <  2:7122610/101‑1 (MQ=255)
                                                      cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                 <  2:6211467/101‑1 (MQ=255)
                                                        cccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAc                                               >  2:1929014/1‑101 (MQ=255)
                                                           gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                            <  2:3710178/101‑1 (MQ=255)
                                                            tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGaa                                           >  1:8356644/1‑101 (MQ=255)
                                                             ccTTCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg                                          <  2:3114771/101‑1 (MQ=255)
                                                                 cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                      >  2:5829443/1‑101 (MQ=255)
                                                                  ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTTC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                     <  1:8117523/101‑1 (MQ=255)
                                                                   gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                    <  1:1988648/101‑1 (MQ=255)
                                                                   gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                    >  2:8861048/1‑101 (MQ=255)
                                                                   caCTCCCCCCGGGATATTTATGGCACGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                    <  2:2348901/100‑1 (MQ=255)
                                                                    aCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                   <  2:1426073/101‑1 (MQ=255)
                                                                    aCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                   >  2:5023458/1‑101 (MQ=255)
                                                                    aCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                   <  1:4663263/101‑1 (MQ=255)
                                                                      tCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGa                                 >  2:158934/1‑101 (MQ=255)
                                                                      tCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGa                                 <  2:4657934/101‑1 (MQ=255)
                                                                        ccccgGGAATATTTAT.GCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                              <  1:6933994/101‑1 (MQ=255)
                                                                          cccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg                             <  2:7451928/101‑1 (MQ=255)
                                                                            cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                           >  1:104331/1‑101 (MQ=255)
                                                                            cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                           <  1:8589459/101‑1 (MQ=255)
                                                                             gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                          >  1:1815322/1‑101 (MQ=255)
                                                                             gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                          >  1:488443/1‑101 (MQ=255)
                                                                               tatatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                        >  2:1764757/2‑101 (MQ=255)
                                                                               gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                        <  1:3054076/101‑1 (MQ=255)
                                                                                atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                       >  1:1383612/1‑101 (MQ=255)
                                                                                 tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                      >  1:5074066/1‑101 (MQ=255)
                                                                                  atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                     <  1:4530410/101‑1 (MQ=255)
                                                                                     tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                  <  2:6607153/101‑1 (MQ=255)
                                                                                     tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                  >  2:3909747/1‑101 (MQ=255)
                                                                                     tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                  >  1:7440856/1‑101 (MQ=255)
                                                                                      aTGGCAAGATGAAACTGG.GGCCTTCATTTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAACATGACAAGATCCGCGCCCCGGcttt                 >  1:398821/1‑101 (MQ=37)
                                                                                      aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                 >  2:4719074/1‑101 (MQ=255)
                                                                                      aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                 >  1:8822968/1‑101 (MQ=255)
                                                                                      aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                 >  1:92692/1‑101 (MQ=255)
                                                                                        ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg               <  2:29678/101‑1 (MQ=255)
                                                                                        ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg               >  1:6859036/1‑101 (MQ=255)
                                                                                        ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg               >  1:2143028/1‑101 (MQ=255)
                                                                                         gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc              >  2:8029697/1‑101 (MQ=255)
                                                                                          cAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt             >  2:8698341/1‑101 (MQ=255)
                                                                                          cAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt             >  2:6028208/1‑101 (MQ=255)
                                                                                           aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg            >  2:3313341/1‑101 (MQ=255)
                                                                                            aGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtga           >  1:6160355/1‑101 (MQ=255)
                                                                                            aGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtga           >  1:7546627/1‑101 (MQ=255)
                                                                                             gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat          >  1:5546298/1‑101 (MQ=255)
                                                                                              aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg         <  1:2721451/101‑1 (MQ=255)
                                                                                               tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGTtgatga        >  1:4161106/1‑101 (MQ=255)
                                                                                                  aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa     >  1:2254797/1‑101 (MQ=255)
                                                                                                   aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa    <  2:4915506/101‑1 (MQ=255)
                                                                                                    cTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg   >  1:7720069/1‑101 (MQ=255)
                                                                                                    cTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg   <  2:8356281/101‑1 (MQ=255)
                                                                                                     |                                                                                                      
CAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGC  >  NC_014034/1734790‑1734989

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG