Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,769 A→G 1.6% intergenic (+6/+129) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,7690A→G 1.6% 4.2 ‑2.9 ‑0.4427intergenic (+6/+129)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/6):  ref base (227/193):  total (228/199)
Fisher's exact test strand distribution p-value = 5.36e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.96e-01

Only 100 of 461 total aligned reads displayed.

CGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGGCGCTGCCCCCCGCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGG  >  NC_014034/1740670‑1740864
                                                                                                    |                                                                                                
 gagaCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.gg                                                                                               >  1:2039082/1‑101 (MQ=255)
 gacaCGAGCGTGCCCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.gg                                                                                               <  1:1509991/98‑1 (MQ=255)
    acgagggTGGCCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.ggggg                                                                                            <  2:2165757/95‑2 (MQ=255)
     cGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.gggggg                                                                                           >  2:194996/1‑99 (MQ=255)
     cGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.gggggc                                                                                           >  2:3967429/1‑99 (MQ=255)
           tGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGcc                                                                                      <  1:5887209/101‑1 (MQ=255)
           tGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGcc                                                                                      <  2:3253809/101‑1 (MQ=255)
           tGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGcc                                                                                      >  2:3593897/1‑101 (MQ=255)
             aCCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGcccc                                                                                    <  1:7821958/101‑1 (MQ=255)
             aCCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGcccc                                                                                    <  1:2597088/101‑1 (MQ=255)
              ccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGccccc                                                                                   <  2:4799768/101‑1 (MQ=255)
              ccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGccccc                                                                                   >  2:5588034/1‑101 (MQ=255)
                  cTGGGGGTTGGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGcccccgccg                                                                               <  1:9016538/101‑5 (MQ=255)
                    gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAg                                                                             <  1:5941753/101‑1 (MQ=255)
                     cGGGTCCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAgg                                                                            <  2:3661919/101‑1 (MQ=255)
                      ggttccGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGc                                                                           <  2:7034042/96‑1 (MQ=255)
                      gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGc                                                                           >  1:2958223/1‑101 (MQ=255)
                      gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGc                                                                           >  1:1378997/1‑101 (MQ=255)
                      gggTTCGAGCCGACGGAAGAAACGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCACGCCGAGGc                                                                           >  1:2500708/1‑101 (MQ=255)
                          tCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTGGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATg                                                                       <  2:6328798/101‑1 (MQ=255)
                           cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGc                                                                      >  2:5815456/1‑101 (MQ=255)
                            gAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGcc                                                                     >  2:2507030/1‑101 (MQ=255)
                             ggCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCt                                                                    <  1:1185911/100‑1 (MQ=255)
                             aGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCt                                                                    >  2:7614249/1‑101 (MQ=255)
                              gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTc                                                                   <  1:5309712/101‑1 (MQ=255)
                              gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTc                                                                   <  2:4978652/101‑1 (MQ=255)
                               ccGACGGAAGAACCGCTTCGGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCg                                                                  <  2:9042081/101‑1 (MQ=255)
                                   cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGccc                                                              >  2:4550400/1‑101 (MQ=255)
                                    ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGcccc                                                             >  2:6183393/1‑101 (MQ=255)
                                     gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGccccc                                                            <  1:991298/101‑1 (MQ=255)
                                      aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGcccccc                                                           <  1:1078377/101‑1 (MQ=255)
                                      aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGcccccc                                                           <  1:448332/101‑1 (MQ=255)
                                      aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGcccccc                                                           >  1:516813/1‑101 (MQ=255)
                                       agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTcagatcggaa                                                          >  2:3102848/1‑92 (MQ=255)
                                       agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCg                                                          >  2:439561/1‑101 (MQ=255)
                                       agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCg                                                          >  2:856293/1‑101 (MQ=255)
                                        gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCgg                                                         >  2:289979/1‑101 (MQ=255)
                                           ccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGat                                                      <  2:7769462/101‑1 (MQ=255)
                                           ccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGat                                                      >  2:8163907/1‑101 (MQ=255)
                                           ccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGat                                                      <  1:5593449/101‑1 (MQ=255)
                                            cGCTTCTGGACTATCTCGAAGGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGata                                                     <  2:413372/101‑1 (MQ=38)
                                             gCTTCGGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGatat                                                    <  2:52983/101‑1 (MQ=38)
                                              cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                   <  1:8230617/101‑1 (MQ=38)
                                              cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                   >  2:6775276/1‑101 (MQ=38)
                                              cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCAGCCCCCCGGGAAAtt                                                   >  1:2831180/1‑101 (MQ=255)
                                               ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                  >  1:7194952/1‑101 (MQ=38)
                                                tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                 >  1:2433309/1‑101 (MQ=38)
                                                tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                 >  2:3467409/1‑101 (MQ=38)
                                                 cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                <  1:8004560/101‑1 (MQ=38)
                                                  tGGACTATCTCGCAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                               >  1:6578111/1‑101 (MQ=38)
                                                  tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                               >  1:4586223/1‑101 (MQ=38)
                                                  tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                               >  1:7440116/1‑101 (MQ=38)
                                                    gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                             <  2:3879466/101‑1 (MQ=38)
                                                     gcTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCa                                            <  2:829428/100‑1 (MQ=38)
                                                       tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                          >  2:8455645/1‑101 (MQ=38)
                                                       tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                          >  2:6633959/1‑101 (MQ=38)
                                                       tATCTCGAAAGCAAAGACGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                          <  2:4201131/101‑1 (MQ=37)
                                                          ctcGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATg                                       <  2:7449587/101‑1 (MQ=37)
                                                          ctcGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATg                                       <  2:3364862/101‑1 (MQ=37)
                                                           tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                      >  1:4679102/1‑101 (MQ=37)
                                                            cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                     >  2:8873253/1‑101 (MQ=37)
                                                             gAAAGCAAATGCGCCGGGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                    <  1:1917993/101‑1 (MQ=37)
                                                             gAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                    <  1:334431/101‑1 (MQ=37)
                                                             gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                    >  2:1215692/1‑101 (MQ=37)
                                                              aaaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                   >  2:7306337/1‑101 (MQ=37)
                                                              aaaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                   <  1:3806772/101‑1 (MQ=38)
                                                               aaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAgg                                  <  1:4179163/101‑1 (MQ=38)
                                                                 gCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                >  1:6982347/1‑101 (MQ=37)
                                                                 gCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                <  1:2179318/101‑1 (MQ=38)
                                                                  cAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                               >  2:1151364/1‑101 (MQ=37)
                                                                   aaaTACGCCGCGATCTGCCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                              <  2:4305545/101‑1 (MQ=37)
                                                                   aaaTACGCCGCGATCTCCCGGCTTTGGGGGG.GAGGGGGCGCCGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                              <  1:3543343/101‑1 (MQ=25)
                                                                   aaaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                              >  1:3670377/1‑101 (MQ=37)
                                                                   aaaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                              >  1:859709/1‑101 (MQ=37)
                                                                    aaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATcc                             >  1:3383774/1‑101 (MQ=37)
                                                                          cccgtaTCTACCGGCTTTGGGGGCGGG.GGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                       <  2:133502/96‑1 (MQ=25)
                                                                            gcgATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                     <  1:9136627/101‑1 (MQ=37)
                                                                            gcgATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                     >  2:1449183/1‑101 (MQ=37)
                                                                             cgATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATc                    <  2:8961854/101‑1 (MQ=37)
                                                                             cgATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATc                    <  1:57584/101‑1 (MQ=37)
                                                                              gATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                   >  1:1703168/1‑101 (MQ=37)
                                                                              gATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                   <  2:3513418/101‑1 (MQ=37)
                                                                              gATCTACCGGCTTGGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                   <  1:6273985/101‑1 (MQ=25)
                                                                               aTCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                  >  1:4813435/1‑101 (MQ=25)
                                                                                tCTCCCGGCTTTGAGGGCGGG.GGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                 <  2:373139/101‑1 (MQ=25)
                                                                                tCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                 >  2:5526337/1‑101 (MQ=25)
                                                                                  tACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt               >  1:8462067/1‑101 (MQ=25)
                                                                                   aCCGGCTTTGGGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt              <  2:5188212/101‑1 (MQ=25)
                                                                                   aCCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt              >  1:6056139/1‑101 (MQ=25)
                                                                                    ccGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc             >  1:644112/1‑101 (MQ=25)
                                                                                     cGGCTTTGGGGGC.GAGGGGGCGCTCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa            <  1:8864862/101‑1 (MQ=21)
                                                                                       gCTTTGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCaaa          <  2:4887646/101‑1 (MQ=25)
                                                                                        cttggggggC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt         <  2:2747963/95‑1 (MQ=21)
                                                                                         tttGGGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa        <  2:8698118/101‑1 (MQ=21)
                                                                                         tttGAGGGC.GAGGGGGCGCGGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa        <  2:8023493/101‑1 (MQ=21)
                                                                                          ttGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc       >  1:3168483/1‑101 (MQ=25)
                                                                                          ttGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc       >  1:2268064/1‑101 (MQ=25)
                                                                                          ttGAGGGC.GAGGGGGCGCTCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc       <  1:4134133/101‑1 (MQ=21)
                                                                                           tGAGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc      >  2:5938570/1‑101 (MQ=25)
                                                                                             aGGGC.GAGGGGGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc    >  2:2373802/1‑101 (MQ=25)
                                                                                                    |                                                                                                
CGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGGCGCTGCCCCCCGCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGG  >  NC_014034/1740670‑1740864

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG