BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,740,785	G→C	98.2%	intergenic (+22/+113)	cxp → / ← RCAP_rcc01610	thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,740,785	0	G→C	98.2%	6.5 ‑2.7 ‑1.3	443	intergenic (+22/+113)	cxp/RCAP_rcc01610	thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand): new base (220/215): ref base (1/7): total (221/222)
Fisher's exact test strand distribution p-value = 6.79e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.57e-02

Only 100 of 457 total aligned reads displayed.


GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.GGG.GCGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880


gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAg                                                                                             <  1:5941753/101‑1 (MQ=255)


gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAg                                                                                             <  2:8017921/101‑1 (MQ=255)


gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAg                                                                                             <  1:5255279/101‑1 (MQ=255)


 cGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAgg                                                                                            >  1:2257729/1‑101 (MQ=255)


   ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCg                                                                                          >  1:8709844/1‑101 (MQ=255)


       cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGc                                                                                      >  2:5028631/1‑101 (MQ=255)


       cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGc                                                                                      >  1:5144894/1‑101 (MQ=255)


         aGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCt                                                                                    <  1:3351910/101‑1 (MQ=255)


           ccGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCg                                                                                  <  2:2019430/101‑1 (MQ=255)


           ccGACGGAAGAACCGCTTCGGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCg                                                                                  <  2:9042081/101‑1 (MQ=255)


            cGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAA.GGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCgg                                                                                 >  1:4670674/1‑101 (MQ=255)


             gACGGAAGAACCGCTTCTGGACTATCGCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGc                                                                                <  2:8952527/101‑1 (MQ=255)


               cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGccc                                                                              >  2:5392743/1‑101 (MQ=255)


                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           <  1:6755134/101‑1 (MQ=255)


                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           <  1:1078377/101‑1 (MQ=255)


                   agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGCGGCGATGCCTCGGCCCCCCg                                                                          <  1:8224101/101‑1 (MQ=255)


                    gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGACCCCCgg                                                                         >  2:6993937/1‑101 (MQ=255)


                     aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                                        >  1:4793211/1‑101 (MQ=255)


                      aCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                                       >  2:3490120/1‑101 (MQ=255)


                      aCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                                       <  2:627814/101‑1 (MQ=255)


                        cGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGata                                                                     >  1:427119/1‑101 (MQ=255)


                         gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                                    <  2:376985/101‑1 (MQ=38)


                         gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                                    >  2:5877200/1‑101 (MQ=255)


                         gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                                    >  2:1048781/1‑101 (MQ=255)


                         ccTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                                    <  2:3057297/100‑1 (MQ=38)


                          cTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                                   <  2:2341663/101‑1 (MQ=38)


                          cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                                   >  2:6775276/1‑101 (MQ=38)


                           ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                  <  1:2492762/101‑1 (MQ=38)


                           ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                  >  1:7194952/1‑101 (MQ=38)


                           ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                  >  2:4134014/1‑101 (MQ=38)


                            tCTGGACTATCTTGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 <  2:4834859/101‑1 (MQ=38)


                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 <  1:8923903/101‑1 (MQ=38)


                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 >  1:2433309/1‑101 (MQ=38)


                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 >  2:3634610/1‑101 (MQ=38)


                             cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                <  1:8004560/101‑1 (MQ=38)


                             cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                >  1:1806607/1‑101 (MQ=38)


                             cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                >  1:2845326/1‑101 (MQ=38)


                             cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                >  1:3449746/1‑101 (MQ=38)


                              tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                                               >  2:6162519/1‑101 (MQ=38)


                               ggACTATCTCGAAAGCAAATCCGCCGCGGTCTTCCGGTTTTGAGGGCGG.G.GGG.GCGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                                              <  1:2021789/101‑1 (MQ=25)


                                gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                                             >  1:9174652/1‑101 (MQ=38)


                                 gcTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCa                                                            <  2:829428/100‑1 (MQ=38)


                                 aCTATCTCGAAAGCAAATGCGCCGCGATCTACGGGCTTTGAGGGC.G.AGGGG.GCGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCa                                                            <  1:1256592/101‑1 (MQ=37)


                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          >  2:4888734/1‑101 (MQ=38)


                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          >  2:8455645/1‑101 (MQ=38)


                                   tATCTCGAAAGCAAAGACGCCGCGATCTCCCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          <  2:4201131/101‑1 (MQ=37)


                                        cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                                     >  1:4517179/1‑101 (MQ=37)


                                         gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    >  2:1201297/1‑101 (MQ=37)


                                          aaaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                                   >  1:5630388/1‑101 (MQ=37)


                                             gCAAATCCGCCGCGATCTCCCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                                <  1:298700/101‑1 (MQ=37)


                                             gCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCACCCGGGATATTTCGAGCAAGATGAAAGGCa                                                >  1:855243/1‑101 (MQ=255)


                                               aaaTACGCCGCGATCTCCCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                              <  2:7379145/101‑1 (MQ=37)


                                               aaaTACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                              >  1:3670377/1‑101 (MQ=37)


                                                aaTACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATcc                                             >  2:6513914/1‑101 (MQ=37)


                                                aaTACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATcc                                             >  1:2597396/1‑101 (MQ=37)


                                                 aTACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                                            >  2:4660332/1‑101 (MQ=37)


                                                   aCGCCGCGATCTACCGGCTTTGAGGGC.G.A.GGGTGCGCTGCCCCCGC.CGTGGGGATGCCTCGGCCCCCCGGGCTATTTCGAGCAAGATGAGAGGCAGCCgga                                          >  2:5581585/1‑100 (MQ=255)


                                                     gccgcGATCTCCCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                                        <  2:2594466/101‑1 (MQ=37)


                                                     gccgcGATCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                                        >  1:1467945/1‑101 (MQ=37)


                                                     gccgcGATCTACCGGCTTGGAGGGC.G.AGGGG.GCCCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                                        <  2:859696/101‑1 (MQ=37)


                                                      cccgtaTCTACCGGCTTTGGGGGCGG.G.GGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                                       <  2:133502/96‑1 (MQ=25)


                                                       cgcgATCTCCCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCa                                      <  1:6044326/101‑1 (MQ=37)


                                                           atttACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  <  2:8126831/98‑1 (MQ=25)


                                                           aTCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  >  2:3152394/1‑101 (MQ=25)


                                                           aTCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  >  2:1680913/1‑101 (MQ=25)


                                                            tCTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                                 >  2:5526337/1‑101 (MQ=25)


                                                             cTACCGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                                >  1:6248919/1‑101 (MQ=25)


                                                               aCCGGCTTTGGGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt                              <  2:5188212/101‑1 (MQ=25)


                                                                ccGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc                             >  1:9036827/1‑101 (MQ=25)


                                                                ccGGCTATGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc                             <  2:8857653/101‑1 (MQ=25)


                                                                 cGGCTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa                            <  2:4488000/101‑1 (MQ=25)


                                                                    cTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt                         >  1:63484/1‑101 (MQ=25)


                                                                    cTTTGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt                         <  1:3816367/101‑1 (MQ=25)


                                                                     tttGAGGGC.G.G.GGGNGCGCGGCCCCCNN.NNNGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa                        <  1:7462732/101‑1 (MQ=21)


                                                                     tttGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa                        >  2:37179/1‑101 (MQ=25)


                                                                     tttGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa                        <  2:7575773/101‑1 (MQ=25)


                                                                      ttGAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc                       >  1:3168483/1‑101 (MQ=25)


                                                                      ttGAGGGC.G.AGGGG.GCGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc                       <  1:4134133/101‑1 (MQ=21)


                                                                        gAGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt                     >  1:6513401/1‑101 (MQ=25)


                                                                         aGGGC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc                    >  2:394932/1‑101 (MQ=25)


                                                                           ggC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgg                  >  1:1128228/1‑101 (MQ=21)


                                                                           ggC.G.AGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgg                  <  2:3566526/101‑1 (MQ=25)


                                                                                gggggg.gCGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               <  1:5526544/98‑1 (MQ=21)


                                                                                gagggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               >  1:9122592/4‑101 (MQ=255)


                                                                                gagggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               >  1:8411983/4‑101 (MQ=255)


                                                                                  gggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGa             <  2:2863006/100‑1 (MQ=255)


                                                                                  gggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGa             <  2:2476361/100‑1 (MQ=255)


                                                                                  gggg.gCGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGa             <  2:1512007/100‑1 (MQ=255)


                                                                                   ggg.gCGTTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAg            <  2:775649/101‑1 (MQ=255)


                                                                                   ggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAg            <  1:5845604/101‑1 (MQ=255)


                                                                                   ggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAg            <  1:2136507/101‑1 (MQ=255)


                                                                                   ggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAg            >  1:1682485/1‑101 (MQ=255)


                                                                                   ggg.gCGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAg            <  2:3420163/101‑1 (MQ=255)


                                                                                      ggcccctccccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGt           <  1:6994222/94‑1 (MQ=18)


                                                                                        cgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTccc        <  2:7194648/101‑1 (MQ=255)


                                                                                        cgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTccc        >  2:3340031/1‑101 (MQ=255)


                                                                                        cgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTccc        <  2:6016420/101‑1 (MQ=255)


                                                                                         gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       >  2:4354482/1‑101 (MQ=255)


                                                                                            gCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAg    >  2:4082294/1‑101 (MQ=255)


                                                                                             cccccgC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAgg   <  1:4594143/101‑1 (MQ=255)


GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.GGG.GCGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG