Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,374 C→G 100% P149R (CCC→CGC)  RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3740C→G100.0% 32.0 289P149R (CCC→CGC) RCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (137/134):  ref base (2/15):  total (139/150)
Fisher's exact test strand distribution p-value = 1.94e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

TCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.C.GGCA.GGGGCGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769284‑1769462
                                                                                                |                                                                                         
 cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCttt                                                                                  <  1:1509896/101‑1 (MQ=255)
    cGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCC.GGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGcc                                                                               <  1:6437056/101‑1 (MQ=255)
    cGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGcc                                                                               >  1:4621912/1‑101 (MQ=255)
     gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCt                                                                              <  1:4489680/101‑1 (MQ=255)
     gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCt                                                                              >  2:2564982/1‑101 (MQ=255)
     gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCt                                                                              >  2:7312195/1‑101 (MQ=255)
     gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCt                                                                              >  1:346395/1‑101 (MQ=255)
     gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTC.GCGCG.CGGGCTTTGCCt                                                                              <  1:4876302/101‑1 (MQ=255)
      aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCtt                                                                             <  1:1822318/101‑1 (MQ=255)
       tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTg                                                                            >  2:1870448/1‑101 (MQ=255)
       tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTC.GCGCG.CGGGCTTTGCCTTg                                                                            <  1:7308695/101‑1 (MQ=255)
        cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                                           >  1:5102384/1‑101 (MQ=255)
         aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTGGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCCTggg                                                                          >  2:6268150/1‑101 (MQ=255)
          ggACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTgggg                                                                         >  2:7982076/1‑101 (MQ=255)
           gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  2:7857174/101‑1 (MQ=255)
           gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        >  1:7050467/1‑101 (MQ=255)
            aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTc                                                                       >  1:3849712/1‑101 (MQ=255)
            aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTc                                                                       <  1:3321671/101‑1 (MQ=255)
            aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTc                                                                       <  1:5046039/101‑1 (MQ=255)
             ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      <  1:2121386/101‑1 (MQ=255)
             ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      <  1:2030183/101‑1 (MQ=255)
              cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGAG.CG.CGGGGTTTGCATTTGGGTCGa                                                                     >  1:4240952/1‑101 (MQ=255)
                aCTGGCTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAgg                                                                   <  2:8817564/101‑1 (MQ=255)
                  tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                 <  2:5781021/101‑1 (MQ=255)
                  tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                 >  1:8994275/1‑101 (MQ=255)
                  tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGGCGAGgcg                                                                 >  1:8225497/1‑101 (MQ=255)
                   ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                                <  1:7265400/101‑1 (MQ=255)
                       gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            >  1:380397/1‑101 (MQ=255)
                       gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  2:7925368/101‑1 (MQ=255)
                        cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           <  2:1774186/101‑1 (MQ=255)
                         ggCCGGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                          <  2:659686/101‑1 (MQ=255)
                          gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCggg                                                         >  1:1580307/1‑101 (MQ=255)
                            ccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCa                                                       >  1:2526432/1‑101 (MQ=255)
                              gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     <  2:3324043/101‑1 (MQ=255)
                              gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     <  2:4240836/101‑1 (MQ=255)
                              gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     <  2:3457294/101‑1 (MQ=255)
                               cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    <  2:6738155/101‑1 (MQ=255)
                                 cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:2074666/101‑1 (MQ=255)
                                 cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:8211618/101‑1 (MQ=255)
                                 cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  >  2:8342229/1‑101 (MQ=255)
                                 cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:4031426/101‑1 (MQ=255)
                                  ggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATg                                                 <  2:2916062/101‑1 (MQ=255)
                                   gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                <  1:4409293/101‑1 (MQ=255)
                                     ggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                                              <  1:402869/101‑1 (MQ=255)
                                     ggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                                              >  1:1334658/1‑101 (MQ=255)
                                       aaaTCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            >  1:5344794/1‑101 (MQ=255)
                                       aaaTCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            >  1:7591227/1‑101 (MQ=255)
                                       aaaTCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            <  1:5527028/101‑1 (MQ=255)
                                       aaaTCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            >  1:8731547/1‑101 (MQ=255)
                                        aaTCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTcc                                           >  2:2177440/1‑101 (MQ=255)
                                         aTCGTCGCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCt                                          <  1:5733758/101‑1 (MQ=255)
                                           cgtcgCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        >  1:3389485/1‑101 (MQ=255)
                                            gtcgCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       >  1:8161286/1‑101 (MQ=255)
                                            gtcgCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       >  2:2685663/1‑101 (MQ=255)
                                            gtcgCGGCTTTGGGCGCGGTCGATG.T.CGGGCACGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       <  2:5012421/101‑1 (MQ=255)
                                             tcgCGGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAt                                      <  2:6100267/101‑1 (MQ=255)
                                              cgcgGCTTTGGGCGCGGTCGATGTT.G.GGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  1:2393379/1‑101 (MQ=255)
                                              cgcgGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     <  1:7825402/101‑1 (MQ=255)
                                              cgcgGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     <  2:36992/101‑1 (MQ=255)
                                               gcgGCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  2:5432902/101‑1 (MQ=255)
                                                 ggCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  >  1:6622866/1‑101 (MQ=255)
                                                 ggCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  >  1:1062929/1‑101 (MQ=255)
                                                  gCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgt                                 <  2:7661729/101‑1 (MQ=255)
                                                  gCTTTGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgt                                 >  1:4157859/1‑101 (MQ=255)
                                                     ttGGGCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                              >  1:1237269/1‑101 (MQ=255)
                                                        ggCGCGGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccgc                           >  1:2949797/1‑101 (MQ=255)
                                                           gcgGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCa                        >  2:3265473/1‑101 (MQ=255)
                                                            cgGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  1:7396209/101‑1 (MQ=255)
                                                            cgGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       >  2:8512823/1‑101 (MQ=255)
                                                            cgGTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  2:519803/101‑1 (MQ=255)
                                                             ggTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      >  1:3533710/1‑101 (MQ=255)
                                                             ggTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      >  1:2872143/1‑101 (MQ=255)
                                                             ggTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      <  1:2242912/101‑1 (MQ=255)
                                                              gTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     >  2:6862426/1‑101 (MQ=255)
                                                              gTCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     <  1:852235/101‑1 (MQ=255)
                                                               tCGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    >  1:1556438/1‑101 (MQ=255)
                                                                cGATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   >  2:4459091/1‑101 (MQ=255)
                                                                 gATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  >  2:6950907/1‑101 (MQ=255)
                                                                 gATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  >  1:5773148/1‑101 (MQ=255)
                                                                 gATG.T.CGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  >  1:1005242/1‑101 (MQ=255)
                                                                     tgtcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGc                <  2:5102215/91‑1 (MQ=255)
                                                                     tgtcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGc                <  2:31428/91‑1 (MQ=255)
                                                                      gtcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt               <  2:421160/92‑1 (MQ=255)
                                                                      gtcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt               >  2:3514980/10‑101 (MQ=255)
                                                                        cgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTgg             >  1:3970047/8‑101 (MQ=255)
                                                                            cagggggCGGTGGTCGCG.CG.CGTGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         >  1:1701713/4‑101 (MQ=255)
                                                                            cagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         >  1:36681/4‑101 (MQ=255)
                                                                            cagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         <  2:410829/98‑1 (MQ=255)
                                                                             agggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        >  2:132538/3‑101 (MQ=255)
                                                                             agggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        >  2:5284685/3‑101 (MQ=255)
                                                                              gggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccga       >  1:7682272/2‑101 (MQ=255)
                                                                               ggggCGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      >  1:8608798/1‑101 (MQ=255)
                                                                                gggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatg     <  2:1580260/101‑1 (MQ=255)
                                                                                gggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatg     <  2:1127414/101‑1 (MQ=255)
                                                                                gggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatg     <  2:2234058/101‑1 (MQ=255)
                                                                                 ggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc    <  1:6098061/101‑1 (MQ=255)
                                                                                 ggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc    <  1:7982416/101‑1 (MQ=255)
                                                                                 ggCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc    >  1:1810214/1‑101 (MQ=255)
                                                                                  gCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACTGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc   >  1:5412430/1‑101 (MQ=255)
                                                                                   cGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgccg  <  2:985074/101‑1 (MQ=255)
                                                                                                |                                                                                         
TCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.T.C.GGCA.GGGGCGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769284‑1769462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG