Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,782,184 T→A 65.5% S661T (TCA→ATA)  RCAP_rcc01644 → FMN‑binding domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,782,1840T→A65.5% 0.0 ‑3.4 0.0359S661T (TCA→ACA) RCAP_rcc01644FMN‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (117/118):  ref base (77/47):  total (194/165)
Fisher's exact test strand distribution p-value = 3.41e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 362 total aligned reads displayed.

TGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782095‑1782275
                                                                                          |                                                                                            
  tgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaa                                                                                 <  2:2654032/101‑1 (MQ=255)
  tgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaa                                                                                 <  1:4433452/101‑1 (MQ=255)
   gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCTCCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaa                                                                                <  1:4348748/101‑1 (MQ=255)
   gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaa                                                                                >  2:2181499/1‑101 (MQ=255)
   gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaa                                                                                <  1:3254422/101‑1 (MQ=255)
    ccGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACaaaa                                                                               <  2:4725936/101‑1 (MQ=255)
    ccGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACaaaa                                                                               <  2:7352391/101‑1 (MQ=255)
    ccGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaaa                                                                               >  2:8670693/1‑101 (MQ=255)
    ccGCGTGCGCTGCGCCAATGGCGCGATCAGGCGCACGGGCGAGATCCGCTATTCCGAGTGTTTCCAGTTCCTTGACTGTGTGGCG.ATCCATGACGACaaaa                                                                               <  1:4918109/101‑1 (MQ=255)
     cgcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAg                                                                              <  2:6168281/101‑1 (MQ=255)
      tcgTGCTCGGCGTCTATGGCGCGCTCAAGGGCACCCGCGAGATCCGCTATTCCGAGTTCTTCGAGTGCCTTGACGGTGTGGCG.ATCCACGACGACAAAAGc                                                                             >  2:5953366/2‑101 (MQ=255)
       cgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGcc                                                                            >  1:3591305/1‑101 (MQ=255)
         tgcgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGc                                                                          <  2:1786307/101‑1 (MQ=255)
          gcgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCt                                                                         <  1:1709838/101‑1 (MQ=255)
           gcttgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtg                                                                        >  2:8951019/4‑101 (MQ=255)
            gctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGATTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgt                                                                       <  1:4782011/101‑1 (MQ=255)
             ctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCtgtg                                                                      <  1:7357804/101‑1 (MQ=255)
             ctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgtg                                                                      >  2:87163/1‑101 (MQ=255)
             ctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGCGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCtgtg                                                                      <  2:7250571/101‑1 (MQ=255)
               gcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCtgtgtg                                                                    <  1:5990654/101‑1 (MQ=255)
                 gcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGcc                                                                  <  1:7073662/101‑1 (MQ=255)
                  ccTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCt                                                                 >  1:676735/1‑101 (MQ=255)
                  ccTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTAGTCCGAGTGTTTCCAGTGCCTTGACTGTCTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCt                                                                 <  1:8261303/101‑1 (MQ=255)
                   cTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCtt                                                                <  2:6042875/101‑1 (MQ=255)
                   cTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCtt                                                                <  2:7845464/101‑1 (MQ=255)
                    tATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCttt                                                               <  1:1178539/101‑1 (MQ=255)
                    tATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCttt                                                               >  2:1029785/1‑101 (MQ=255)
                     aTGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTg                                                              <  1:138889/101‑1 (MQ=255)
                          gcgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCt                                                         >  1:8422747/1‑101 (MQ=255)
                           cgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTg                                                        >  2:2975713/1‑101 (MQ=255)
                             aTCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggc                                                      <  1:4192183/101‑1 (MQ=255)
                             aTCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggc                                                      <  1:8671078/101‑1 (MQ=255)
                             aTCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggc                                                      <  2:4938159/101‑1 (MQ=255)
                              tCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcg                                                     <  2:9089716/101‑1 (MQ=255)
                               cAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcgg                                                    >  1:5152682/1‑101 (MQ=255)
                               cAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcgg                                                    >  2:5353843/1‑101 (MQ=255)
                                aggcgcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcggc                                                   <  1:8951440/99‑1 (MQ=255)
                                aaGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcggc                                                   >  1:4091517/1‑101 (MQ=255)
                                aaGCGCACCGGCGAGATCCGCTATTCCGAGAGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcggc                                                   >  1:260087/1‑101 (MQ=255)
                                 aGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGgcggcg                                                  <  2:6413034/101‑1 (MQ=255)
                                   cgcACCGGCGTGATCCGCTATTACGAGTGTTTCCAGAGCCTTGACTGTGTGGCG.ATCCATGACGACACAAGCCGCTGTATCCCGTAGGTGCTGacggcgca                                                >  2:1317127/1‑99 (MQ=255)
                                    gcACCGCCGAGAGCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGGGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaa                                              <  1:2978864/101‑2 (MQ=255)
                                     cACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaac                                             <  1:7658719/101‑3 (MQ=255)
                                      aCCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacg                                            <  2:6609199/101‑4 (MQ=255)
                                        cGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacgaa                                          >  2:1511825/1‑96 (MQ=255)
                                           cGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAgg                                        >  1:2210461/1‑101 (MQ=255)
                                           cGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAgg                                        <  2:5810879/101‑1 (MQ=255)
                                            gagaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGc                                       >  1:3639455/1‑101 (MQ=255)
                                             agaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCt                                      >  1:3124646/1‑101 (MQ=255)
                                             agaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCt                                      >  2:501860/1‑101 (MQ=255)
                                              gaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTc                                     >  1:2436896/1‑101 (MQ=255)
                                               aTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCg                                    >  2:2431957/1‑101 (MQ=255)
                                               aTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCg                                    <  1:1975325/101‑1 (MQ=255)
                                               aTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCg                                    >  2:1397443/1‑101 (MQ=255)
                                                 ccGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCggg                                  >  2:8313595/1‑101 (MQ=255)
                                                 ccGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCggg                                  >  2:797612/1‑101 (MQ=255)
                                                 ccGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCggg                                  <  2:4017566/101‑1 (MQ=255)
                                                    cTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCAt                               >  2:7165415/1‑101 (MQ=255)
                                                    cTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCAt                               >  2:4296664/1‑101 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              >  1:7929624/1‑101 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              >  1:1813390/1‑101 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              <  2:529653/101‑1 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              <  1:5840017/101‑1 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              <  1:5679254/101‑1 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              <  2:6736025/101‑1 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              <  1:276664/101‑1 (MQ=255)
                                                      aTTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGa                             >  1:6397457/1‑101 (MQ=255)
                                                      aTTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCgggagatc                             >  1:2963468/1‑96 (MQ=255)
                                                      aTTCCGAGTGTGTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGGGCTGGCGGCGAAACGAAAGGCTCGGGCATGa                             <  2:1669592/101‑1 (MQ=255)
                                                       ttCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            <  1:6121715/101‑1 (MQ=255)
                                                       ttCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            <  1:887137/101‑1 (MQ=255)
                                                        tCCGAGTGTTTCCAGTGCCTGGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAg                           <  2:8418886/101‑1 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgc                          >  1:2174017/1‑101 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGGTGGCGGCGAAACGAAAGGCTCGGGCACGAAgg                          >  1:4384093/1‑100 (MQ=255)
                                                          cGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcg                         >  2:2566494/1‑101 (MQ=255)
                                                          cGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcg                         <  1:692137/101‑1 (MQ=255)
                                                            aGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggc                       <  1:1029803/101‑1 (MQ=255)
                                                             gtgtTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcg                      >  1:1459284/1‑101 (MQ=255)
                                                             gtgtTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcg                      <  1:5019781/101‑1 (MQ=255)
                                                               gtTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggc                    >  2:588811/1‑101 (MQ=255)
                                                               gtTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggc                    <  1:3944105/101‑1 (MQ=255)
                                                                tttCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggcg                   <  2:3811069/101‑1 (MQ=255)
                                                                  tCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGt                 >  1:2670502/1‑101 (MQ=255)
                                                                    cAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGttt               >  1:7001270/1‑101 (MQ=255)
                                                                      gTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCt             >  2:1123506/1‑101 (MQ=255)
                                                                       tGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTg            <  2:541336/101‑1 (MQ=255)
                                                                        gCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGc           >  2:2068640/1‑101 (MQ=255)
                                                                          cTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAg         <  2:3124547/101‑1 (MQ=255)
                                                                            tGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGAt       >  1:3112912/1‑101 (MQ=255)
                                                                            tGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGAt       >  1:964864/1‑101 (MQ=255)
                                                                            tGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGAt       >  1:5442912/1‑101 (MQ=255)
                                                                            tGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGGTTCTGCAGAt       >  2:6296439/1‑101 (MQ=255)
                                                                             gACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATc      <  1:5299317/101‑1 (MQ=255)
                                                                             gACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATc      >  1:4612972/1‑101 (MQ=255)
                                                                             gACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATc      >  2:5605603/1‑101 (MQ=255)
                                                                               cTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTC.GGCATGAAGCGGCGGCGGTTTCTGCAGATCGTg   <  1:8538607/101‑1 (MQ=255)
                                                                                tgtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTg   <  2:6877689/101‑1 (MQ=255)
                                                                                tgtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTg   >  1:809785/1‑101 (MQ=255)
                                                                                tgtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTg   >  2:3543865/1‑101 (MQ=255)
                                                                                 gtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTgg  >  2:2859241/1‑101 (MQ=255)
                                                                                          |                                                                                            
TGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782095‑1782275

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG