Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,960,669 G→C 3.9% G370G (GGC→GGG)  RCAP_rcc01813 ← AMP‑dependent synthetase and ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,960,6690G→C 3.9% 27.7 ‑0.5 ‑0.0435G370G (GGC→GGG) RCAP_rcc01813AMP‑dependent synthetase and ligase
Reads supporting (aligned to +/- strand):  new base (9/8):  ref base (195/223):  total (204/231)
Fisher's exact test strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.64e-01

Only 100 of 458 total aligned reads displayed.

GATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GG.CTCGGCGGCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCTCCGGGGGACAGGACCGCGA  >  NC_014034/1960572‑1960769
                                                                                                   |                                                                                                    
gATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGCGCcc                                                                                                    >  1:1136976/1‑101 (MQ=255)
gATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGCGCcc                                                                                                    >  1:6347789/1‑101 (MQ=255)
gATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGCGCcc                                                                                                    <  2:46085/101‑1 (MQ=255)
gATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGCGCcc                                                                                                    >  2:2905833/1‑101 (MQ=255)
gATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGGGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCTGC.CTCGGCGCcc                                                                                                    >  1:9043123/1‑101 (MQ=255)
  tAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGCGCccca                                                                                                  <  1:6142939/101‑2 (MQ=255)
    ggCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAAt                                                                                               <  1:1392711/101‑1 (MQ=255)
    ggCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAAt                                                                                               <  2:2386589/101‑1 (MQ=255)
     gCGGTGGCGCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATa                                                                                              <  1:7427189/101‑1 (MQ=255)
     gCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATa                                                                                              <  1:5905565/101‑1 (MQ=255)
     gCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATa                                                                                              <  2:2999616/101‑1 (MQ=255)
     gCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATa                                                                                              <  2:7126334/101‑1 (MQ=255)
     gCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATa                                                                                              <  2:7969226/101‑1 (MQ=255)
      cGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCTGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAg                                                                                             <  1:6763632/101‑1 (MQ=255)
       gATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGc                                                                                            >  1:8688020/1‑101 (MQ=255)
       gATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGc                                                                                            >  1:3269305/1‑101 (MQ=255)
        gtGGCCCCGGCGTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGcc                                                                                           <  2:3257007/100‑1 (MQ=255)
         tGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCg                                                                                          >  2:1235817/1‑101 (MQ=255)
         tGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCg                                                                                          >  2:7692428/1‑101 (MQ=255)
         tGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCg                                                                                          >  2:9183425/1‑101 (MQ=255)
          ggCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGa                                                                                         >  2:8748721/1‑101 (MQ=255)
          ggCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGa                                                                                         >  1:8496730/1‑101 (MQ=255)
             cccGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGa                                                                                      <  1:7139111/101‑1 (MQ=255)
               cGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGaaa                                                                                    <  1:2472142/101‑1 (MQ=255)
                ggCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGaaaa                                                                                   >  1:101609/1‑101 (MQ=255)
                ggCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGaaaa                                                                                   <  2:7991058/101‑1 (MQ=255)
                ggCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGaaaa                                                                                   <  2:6179633/101‑1 (MQ=255)
                  ccTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGc                                                                                 <  1:6941487/101‑1 (MQ=255)
                  ccTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGc                                                                                 <  2:9113510/101‑1 (MQ=255)
                     cGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGcccc                                                                              >  2:5495669/1‑101 (MQ=255)
                     cGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGcccc                                                                              >  2:8986879/1‑101 (MQ=255)
                      ggTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGCAAAGCCCCg                                                                             >  2:7369920/1‑101 (MQ=255)
                      ggTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCg                                                                             >  2:536465/1‑101 (MQ=255)
                      ggTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCg                                                                             >  1:4037511/1‑101 (MQ=255)
                       gTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCgg                                                                            <  1:606437/101‑1 (MQ=255)
                        tCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGa                                                                           <  1:931529/101‑1 (MQ=255)
                        tCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGa                                                                           >  2:6367476/1‑101 (MQ=255)
                        tCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGa                                                                           <  1:2266171/101‑1 (MQ=255)
                         cATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGAt                                                                          >  2:5041779/1‑101 (MQ=255)
                          aTCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATc                                                                         >  2:3557247/1‑101 (MQ=255)
                           tCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCg                                                                        >  2:4904824/1‑101 (MQ=255)
                             cGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCt                                                                      <  1:550793/101‑1 (MQ=255)
                              gCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTg                                                                     <  2:1876984/101‑1 (MQ=255)
                              gCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTg                                                                     >  2:2477494/1‑101 (MQ=255)
                                 cgaggacGCCGGTGCAGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCaa                                                                  >  1:7243249/7‑101 (MQ=255)
                                  gATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAAt                                                                 <  2:5584798/101‑1 (MQ=255)
                                    tGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGc                                                               <  1:7969834/101‑1 (MQ=255)
                                    tGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGc                                                               <  2:7234166/101‑1 (MQ=255)
                                     gTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCa                                                              >  1:161289/1‑101 (MQ=255)
                                     gTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCa                                                              <  2:4549361/101‑1 (MQ=255)
                                     gTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCa                                                              >  1:8904770/1‑101 (MQ=255)
                                     gTCGCCGGCGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCGCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCCCTGCAATGcc                                                              >  2:4445835/1‑100 (MQ=255)
                                       cGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCAcc                                                            <  1:6289939/101‑1 (MQ=255)
                                         ccGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCACAATAGCCGCGGAAAAGCCCCGGGTCGCTGCAATGCACCgc                                                          >  1:2588672/1‑100 (MQ=255)
                                          cGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGAc                                                         >  1:5207004/1‑101 (MQ=255)
                                          cGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGAc                                                         <  2:1571859/101‑1 (MQ=255)
                                          cGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGAc                                                         <  2:1051851/101‑1 (MQ=255)
                                           ggTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACa                                                        <  1:5298764/101‑1 (MQ=255)
                                           ggTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACa                                                        >  1:2172013/1‑101 (MQ=255)
                                             tGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGc                                                      >  1:3896781/1‑101 (MQ=255)
                                                ggAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCg                                                   >  1:4849454/1‑101 (MQ=255)
                                                   aCCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGccc                                                <  2:6323776/101‑1 (MQ=255)
                                                     cAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCgg                                              >  2:521376/1‑101 (MQ=255)
                                                        tCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTc                                           <  1:4168103/101‑1 (MQ=255)
                                                        tCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTc                                           <  1:831814/101‑1 (MQ=255)
                                                         ccccGTCCGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCg                                          <  2:1766629/101‑1 (MQ=255)
                                                               ccGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCgg                                    >  2:8606279/1‑101 (MQ=255)
                                                               ccGCGGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCgg                                    >  1:765002/1‑101 (MQ=255)
                                                                  cgGTGATCTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGGCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGc                                 <  1:7510684/101‑1 (MQ=255)
                                                                  cgGTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGc                                 >  2:8396085/1‑101 (MQ=255)
                                                                   ggTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCa                                >  1:2369518/1‑101 (MQ=255)
                                                                    gTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCAc                               <  1:4488678/101‑1 (MQ=255)
                                                                    gTGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCAc                               >  1:4613707/1‑101 (MQ=255)
                                                                     tGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCAcc                              <  1:1987142/101‑1 (MQ=255)
                                                                     tGAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCAcc                              <  1:8065167/101‑1 (MQ=255)
                                                                      gAACTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCg                             >  1:6582494/1‑101 (MQ=255)
                                                                         cTTTGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGGTGCAATGCAAGGAGGGGTCGCGGGGTTCGGCCGGGGGGAcccggt                          >  1:4296852/1‑97 (MQ=255)
                                                                          tttGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATg                         >  2:4287007/1‑101 (MQ=255)
                                                                           ttGCCCGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATgg                        <  1:5072915/101‑1 (MQ=255)
                                                                              cccGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGCGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGTTTCGCCCCGGGGCACCGGATGGccc                     <  2:2588590/101‑2 (MQ=255)
                                                                               ccGCGCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGccct                    <  2:5851497/101‑3 (MQ=255)
                                                                                cgcgCCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGccctc                   >  1:3168893/1‑98 (MQ=255)
                                                                                 gcgcCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGccctcg                  <  1:4970468/101‑5 (MQ=255)
                                                                                   gcCTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCggg                >  2:7603166/1‑101 (MQ=255)
                                                                                    ccTC.GGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCgggg               >  2:6713422/1‑101 (MQ=255)
                                                                                     ctc.ggcctcgGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCggggg              <  2:2212329/101‑1 (MQ=255)
                                                                                     ctc.ggcctcgGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCggggg              >  2:5988260/1‑101 (MQ=255)
                                                                                       tcggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGa             <  1:3721696/96‑1 (MQ=255)
                                                                                       tcggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGa             >  2:164030/6‑101 (MQ=255)
                                                                                        cggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGAc            <  1:5796282/97‑1 (MQ=255)
                                                                                        cggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGAc            <  2:3896666/97‑1 (MQ=255)
                                                                                         ggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACa           >  2:3432448/4‑101 (MQ=255)
                                                                                          gcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAg          >  2:2139038/3‑101 (MQ=255)
                                                                                           cctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAgg         <  1:1478011/100‑1 (MQ=255)
                                                                                           cctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAgg         <  1:1810787/100‑1 (MQ=255)
                                                                                            gcgcggcgcCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAgg         <  2:6558195/94‑1 (MQ=255)
                                                                                            ctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAGGa        >  2:5692686/1‑101 (MQ=255)
                                                                                            ctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAGGa        <  1:1235849/101‑1 (MQ=255)
                                                                                            ctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAGGa        >  1:3003363/1‑101 (MQ=255)
                                                                                                ggcgcCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAGGACcg     <  1:7809255/98‑1 (MQ=255)
                                                                                                   |                                                                                                    
GATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTC.GG.CTCGGCGGCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCTCCGGGGGACAGGACCGCGA  >  NC_014034/1960572‑1960769

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG