Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,965,271 C→G 100% intergenic (+39/+53) bdhA → / ← RCAP_rcc01818 3‑hydroxybutyrate dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,965,2710C→G100.0% 247.6 102intergenic (+39/+53)bdhA/RCAP_rcc018183‑hydroxybutyrate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (61/41):  ref base (0/0):  total (61/41)

Only 100 of 132 total aligned reads displayed.

CGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GCGGGCT.C.C.G.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTC  >  NC_014034/1965174‑1965366
                                                                                                        |                                                                                                   
 ggcggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGgggg                                                                                                 >  1:5496589/1‑100 (MQ=255)
 ggcggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGc                                                                                                 >  2:1120463/1‑101 (MQ=255)
   cggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GGGG.CG.ACCCG.CC..CGGGGCT.c                                                                                              >  2:1114634/1‑101 (MQ=39)
    ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGgggggcc                                                                                              >  2:1965779/1‑97 (MQ=255)
      cgcAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGT.G.C.G.c                                                                                        >  2:8181747/1‑101 (MQ=38)
      cgcAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCG.C.C.G.c                                                                                        >  1:7583846/1‑101 (MQ=255)
        cAGGTCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCG.C.C.C.ccc                                                                                      >  1:7229250/1‑101 (MQ=38)
        cAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG..C..GCggggggccgccc                                                                                         >  2:2114038/1‑92 (MQ=37)
         aGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGG.C.C.C.cccc                                                                                     >  1:4735328/1‑101 (MQ=38)
          gATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGggggggccccccc                                                                                        >  1:3620908/1‑91 (MQ=38)
             cACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGG.C.C.C.cccccccg                                                                                 >  2:410114/1‑97 (MQ=37)
             cACCGGCACGACGATGTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGC.C.C.G.ccccccgc                                                                                 >  1:7239045/1‑97 (MQ=25)
              aCCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..CGGGGGG.C.C.G.cccccccgc                                                                                >  2:2143392/1‑96 (MQ=37)
               ccGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGT.C.C.G.ccccccggcc                                                                               >  1:1627867/1‑95 (MQ=37)
                   cACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGC.C.C.G.ccccccccgcaccc                                                                           >  2:3264099/1‑91 (MQ=25)
                     cgacgaTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..CGGGGCT.CGG.G.CCCCGGTCCCCCGAg                                                                          >  2:501321/1‑101 (MQ=37)
                      gacgaTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..CGGGGGT.C.C.GCCCCCGGACCCCCGAgg                                                                         >  2:5963352/1‑101 (MQ=37)
                         gaTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTTGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGT.C.C.GCCCCCGGGGCCCCGAGGGAt                                                                      >  1:2989998/1‑101 (MQ=25)
                          aTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCGCCC..GGGGGCC.C.C.C.CCCCCGGccccccgggggat                                                                     >  1:6586114/1‑93 (MQ=25)
                          aTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..CGGGGCT.C.C.GCCCCCCGACCCCCGAGGGAtt                                                                     >  1:2802488/1‑101 (MQ=25)
                            ttCTGCGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCTCC.C.C.CCCCGGACCCCCGAgggagtgc                                                                   >  2:8970865/1‑95 (MQ=21)
                             tCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGT.C.C.GCCCCCCGGCCCCCCAGGGATTTCa                                                                  >  2:3288021/1‑101 (MQ=21)
                              gtggggggggcggcGGCGCGGCTCGGTGAGAGTTCGCGCCGG.GGGCCAGCCCC.GGGC.CC.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAg                                                                 <  2:5936072/93‑1 (MQ=2)
                              cTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCTCC.C.C.CCCCGGACCCCCGAGGGATTTccg                                                                 >  2:4280292/1‑99 (MQ=25)
                               tgtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGT.T.C.GCCCCCCGGCCCCCCCGGTATTTTAgg                                                                >  2:6616805/1‑101 (MQ=18)
                               tgtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGCGGGATTTCAgg                                                                >  2:1662911/1‑101 (MQ=25)
                                gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CCCGGGGGGCC.C.C.C.CCCCGGCCCCCCCAggacttctcgg                                                                >  1:5870839/1‑92 (MQ=21)
                                gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGTCC.C.C.CCCCCGGCCCCCCAGGGGTTTCAGGc                                                               >  1:5802370/1‑101 (MQ=21)
                                gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCTCC.C.C.CCCCCGACCCCCGCGGGATTTCCGGc                                                               >  1:2249429/1‑101 (MQ=21)
                                 tgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGT.C.C.GCCCCCGGACCCCCCGGGGATTTCAGGCa                                                              >  2:5636575/1‑101 (MQ=21)
                                 tgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCC.C.C.GCCCCCCGGCCCCCCGGGGATTTCCGGCa                                                              >  1:7108094/1‑101 (MQ=18)
                                   gggggcggcGGGCGGGCGTGGTGAGGGTTTCCGCCGG.GGGGCACCCCC.GGGC.CC.CCCCG.CC..GGGGGCT.C.T.GCCCCCGGACCCCCGAGGTATTTCAGGCAAg                                                            <  1:6107488/98‑1 (MQ=2)
                                   gATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGT.C.C.GCCCCCGGGCCCCCGAGGGATTTCAGGCAAg                                                            >  2:7276820/1‑101 (MQ=25)
                                    gggggggcGGGGCGGGGCTGGGGGAGCTTCCGCCGG.GGGGCACGCCG.GGGG.CC.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGa                                                           <  2:5642761/96‑1 (MQ=9)
                                    ggggcggcGGGGCGGCGCTGTGAAAGTTTCCCCCGC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGa                                                           <  1:473817/99‑1 (MQ=18)
                                    aTGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGGCCCCCCGGGGATTTCCgggcagg                                                           >  2:5237564/1‑96 (MQ=18)
                                     tGGCGGCTGGACGGCGCTGTGAGAGATATCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGT.C.CGG.CCCCCGCCCCCCGCGGGAtttgcagccagaa                                                          >  2:2112683/1‑91 (MQ=16)
                                     gggcgggGGGCCGGCGCGGGGAGAG.TTGCCCCCG.GGGGCCAGCCC.GGGGCCC.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGAt                                                          <  1:2577204/100‑1 (MQ=2)
                                     gggcggcGGGCGGGGGTTGTGAGAGTTTCCCCCGG.GGGGCACGCCG.GGGG.CC.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGAt                                                          <  2:5539946/100‑1 (MQ=9)
                                      ggcggcTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGGATTTCAGGGCAGATg                                                         >  2:6675437/1‑101 (MQ=25)
                                      ggcggcGGGGGGGCGGGGTGAGAGTTCCCGCCGG.GGGCCAGCCCC.GAGG.CG.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATg                                                         <  2:7690983/101‑1 (MQ=2)
                                        gggggggggggCGGTGTGAGAGCTTCGGCCGG.GGGGCAGGCCG.GGGG.CC.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGaa                                                       <  2:2964024/92‑1 (MQ=9)
                                         ggcTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGGATTTCAGGCAAGATGaaa                                                      >  1:1299692/1‑101 (MQ=21)
                                          gggggcgggCGGTGGGAGAGTTTCCGCCGG.GGGCCAACCCG.GGGC.CC.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAag                                                     <  2:4718566/94‑1 (MQ=2)
                                           cTGGACGGCGCTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CCGGGGGGGCC.C.C.C.CCCCGGCCCCCCCGGGGTTTTCAGCGAAGAGGaaaa                                                     >  1:2513049/1‑100 (MQ=12)
                                              gggggCGGTGTGAGAG.TTCCCCCGCGGGGGCACCCCC.GAGC.CC.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAggg                                                 <  2:3614019/98‑1 (MQ=9)
                                                  gatcTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGTCC.C.C.CCCCCGACCCCCGAGGGATTTCAGGCCAGATTGAAGAGGGgggc                                             >  1:8543887/4‑101 (MQ=16)
                                                    gcTGTGAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGTCC.C.C.CCCCCGGCCCCCGCGGGATTTCAGGCAAGAAGGAAGAGGGgcgggc                                           >  2:3458240/1‑98 (MQ=16)
                                                      tgtgAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCGCCC..GGGGGCC.C.C.C.CCCCCGGCCCCCCCGGGGTTTTAAGGGAGAAGGAAGAGGGGGGCTCAg                                         >  1:5986250/1‑101 (MQ=9)
                                                       gtgAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGTCC.C.C.CCCCCGGCCCCCCAGGGATTTTCGGGAAGATGAAAGAGGGgggccccgg                                        >  2:6929686/1‑96 (MQ=12)
                                                       gtgAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGT.C.C.GCCCCCGGGCCCCCCAGGGATTTTAGGCAAGATGAAAGAggggggggcagg                                        >  2:7787975/1‑93 (MQ=18)
                                                        tgAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGCGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAggg                                       >  2:8295888/1‑100 (MQ=21)
                                                        tgAGAGATTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCCggg                                       >  2:5277745/1‑100 (MQ=21)
                                                         gagagaTTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGT.C.C.GCCCCCGGGCCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCCGAggcg                                      >  2:3666811/1‑101 (MQ=21)
                                                          agagaTTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGG.C.C.GCCCCCCGCCCCCCGAGGAATTTCAGGCAAGATGAAAGAGGGGCGGGCAggggg                                     >  2:115514/1‑101 (MQ=18)
                                                          agagaTTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAggcgg                                     >  2:1545964/1‑101 (MQ=21)
                                                          agagaTTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCCAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCCggcgg                                     >  2:8234648/1‑101 (MQ=21)
                                                           gagaTTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGCACCCCCCCGGGATTTCAGGCAAGATGAAAGAGGGGGGCTCAggccgc                                    >  2:8400765/1‑101 (MQ=18)
                                                            agaTTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CCG.GGGGGCC.C.C.C.CCCCCGGCCCCCCCGGGATTTTAGGCAAAATGAAAGAGGGGGGCTCAGGCGGcc                                   >  1:963026/1‑101 (MQ=16)
                                                             gaTTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGTCC.C.C.CCCCGGGCCCCCCCGGGATTTCTGGCAAGATGGAAGAGGGGGGCGCAGGGGGCct                                  >  1:8755106/1‑101 (MQ=12)
                                                              aTTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCCAGGGATTTCAGGCAAGATGAAAGAGGGGCGCCCAGGCGGCctt                                 >  1:5798850/1‑101 (MQ=21)
                                                              aTTTCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCCGGCCCCCCAGGGCTTTCGGGCAAGATGAAAAAGGGGGGCTCAGGCGGcccc                                 >  1:8385315/1‑99 (MQ=16)
                                                               tctccgcgc.GGGGCAACCCCGGCGG.CC.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCctt                                 <  2:8669000/93‑1 (MQ=18)
                                                                 tcgcgcggggggCCGGCCC.GGGG.CGCCCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCcttc                                <  2:5476407/93‑1 (MQ=18)
                                                                  cgcGTC.GGGGCAAGCGC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGAGCGCTCAGGCGGCCttcttc                             >  1:8825620/1‑101 (MQ=21)
                                                                   cccgc.GGGGCACGCCC.GGGC.CC.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTcg                            <  1:613858/97‑1 (MQ=21)
                                                                    gccc.GGGGCACCCCCGGACG.CC.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTcg                            <  1:5419641/98‑1 (MQ=18)
                                                                    ccgc.GGGGCCACCCCGGAGC.CG.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTcg                            <  2:8094216/98‑1 (MQ=18)
                                                                      ccgggggCCAGCCC.GGGGCCC.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTcgc                           <  2:5360321/98‑1 (MQ=21)
                                                                       c.GGGGCAAGCCC.GAGG.CG.ACCCG.CCG.GGGGGCC.C.C.C.CCCCCGGCCCCCCCGGGATTTTAGGGCAGAAGAAAAAAGGGGGCGCCGGGGGGCCTCCTcgcccc                        >  2:558092/1‑99 (MQ=2)
                                                                       c.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGGT.C.C.GCCCCCCGACCCCCCAGGGATTTCCGGCAAGATGGAAGAGGGGGGCTCAGGCGGCCTTTCTcgccgc                        >  2:8142677/1‑101 (MQ=21)
                                                                       c.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGGGCTCAGGCGGCCTTCTTcgccgc                        >  2:9003112/1‑101 (MQ=21)
                                                                       c.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCCGGCCCCCCCGGGATTTCAGGCCAGATGAAAGAGGGGCGCTCAGGCGGCCCTCTTcgccgc                        >  1:1493935/1‑101 (MQ=21)
                                                                        ggggcCAAGCCC.GGCG.CC.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTcgccgc                        <  1:861366/100‑1 (MQ=21)
                                                                         ggggCAAGCCC.GAGG.CG.ACCCG.CCG.GGGGGGC.C.C.C.CCCCCGACCCCCCCGGGATTTCAGGCAAAAAGAAAAAAGGGCGCTTCGGCGGCCTTCTTCgccccc                       >  2:2724938/1‑101 (MQ=12)
                                                                          gggCAAGCCC.GAGG.CG.ACCCG.CC..GGGGGCTCC.C.C.CCCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCgccgccc                      >  2:8235987/1‑100 (MQ=25)
                                                                              aaGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCCTCTTCGCCGCCTTGcc                  >  2:1371645/1‑101 (MQ=21)
                                                                              aaGCCC.GAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCCGAGCCCCCCGGTATTTCAGGCAAGATGAAAGAGGGGCGGGCGGGCGGGCTTCCTCcccccccgtcc                  >  2:2834626/1‑96 (MQ=9)
                                                                                 ccc.GAGG.CG.ACCCG.CC..GGGGGCTCC.C.C.CCCCCGACCCCCCAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCCTGccccg               >  2:7044733/1‑99 (MQ=21)
                                                                                   c.GAGC.CG.CCCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgtg             <  2:3202607/101‑1 (MQ=25)
                                                                                     gAGG.CG.ACCCG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgtgt            >  2:7238006/1‑101 (MQ=25)
                                                                                     ccggccccccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgt              <  1:1943294/92‑1 (MQ=25)
                                                                                     ccgcggccccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgt              <  1:3687327/92‑1 (MQ=25)
                                                                                      aGG.CG.ACCCG.CC..GGGGGGT.C.C.GCCCCCCGACCCCCGAGGGATTTCCGGCAAGATGAAAGAGGGGCGCCCAGGCGGCCCTCTTCGCCGCCCTGCCCCgtggc           >  2:2894662/1‑99 (MQ=18)
                                                                                        gggccccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtc           <  2:4123805/95‑1 (MQ=25)
                                                                                         gccgccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtct          <  2:8972161/96‑1 (MQ=25)
                                                                                         gcccccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtct          <  2:8754701/96‑1 (MQ=25)
                                                                                         ccccccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtct          <  2:1995825/96‑1 (MQ=25)
                                                                                          cccccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtctc         <  1:2069087/97‑1 (MQ=25)
                                                                                          cccccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtctc         <  1:131192/97‑1 (MQ=25)
                                                                                          cccccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtctc         <  1:5277929/97‑1 (MQ=25)
                                                                                          cccccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtctc         <  1:5406111/97‑1 (MQ=25)
                                                                                           ccccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCg        <  1:8056243/98‑1 (MQ=37)
                                                                                           ccccccG.CC..GGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCg        <  2:7107790/98‑1 (MQ=37)
                                                                                            cccccG.CC..GGGGGCT.T.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCgg       <  1:8000229/99‑1 (MQ=37)
                                                                                               ccg.cc..gGGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGct    <  2:7401024/101‑1 (MQ=255)
                                                                                                cg.cc..ggGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGctt   <  1:8752861/101‑1 (MQ=255)
                                                                                                 g.cc..ggGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGcttc  <  2:7718799/101‑1 (MQ=255)
                                                                                                 g.cc..ggGGGCT.C.C.GCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGcttc  <  2:3831917/101‑1 (MQ=255)
                                                                                                 g.cc..ggGGGCC.C.C.GCCCCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCCTCTTCGCCGCCTTGCCCGGGGTCTCGGGgttc  >  2:6491933/1‑101 (MQ=25)
                                                                                                        |                                                                                                   
CGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTC.GGGGCAAGCCC.GAGG.CG.ACCCG.CC..GCGGGCT.C.C.G.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTC  >  NC_014034/1965174‑1965366

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 26 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG