Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,976,892 G→C 93.3% intergenic (‑71/+9) fba ← / ← gap1 fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,976,8920G→C93.3% 65.5 ‑0.2 ‑0.0477intergenic (‑71/+9)fba/gap1fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (229/215):  ref base (16/17):  total (245/232)
Fisher's exact test strand distribution p-value = 8.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.15e-01

Only 100 of 493 total aligned reads displayed.

GTTGCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCGGCGTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976797‑1976990
                                                                                                  |                                                                                                  
gTTGCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCGACC.CCGGctt                                                                                                >  2:7785016/1‑99 (MQ=255)
   gCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTtc                                                                                            >  1:6202317/1‑101 (MQ=255)
     caaaGGGTGATCGCAGCTATTTTGTCAGTTCCTGGAGTTCGCGGCTTTTTTCTTGGCACAAATACGCCGCGGGT.CCGGGGGCAGCG.CCACCGGC.TTTtctc                                                                                          <  2:5557304/99‑1 (MQ=12)
     cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTtctc                                                                                          >  1:500263/1‑101 (MQ=255)
     cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTtctc                                                                                          >  1:2679243/1‑101 (MQ=255)
      gaggggTGATCAGTGCCTTTTTCTCAGTCCCTTGATTTCGCGCCCTTTTTCTTGGCCGAAATCCGCGGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCa                                                                                         <  2:9197235/98‑1 (MQ=21)
        aGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGa                                                                                       >  2:7523585/1‑101 (MQ=255)
          ggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATc                                                                                     <  2:4623541/101‑1 (MQ=255)
            tGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAg                                                                                   <  2:3615993/101‑1 (MQ=255)
            tGAGCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATCCGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAg                                                                                   <  2:4794212/101‑1 (MQ=25)
             gATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGc                                                                                  >  1:2101794/1‑101 (MQ=255)
             gATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGc                                                                                  >  1:3167041/1‑101 (MQ=255)
               tCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCtt                                                                                >  1:8278843/1‑101 (MQ=255)
                gaGTGCCTTTTTCTCGTTTCTTGGAGTCCGCGCCTTTTTTCTTGCCCAAAATACGCCGGGGGC.CGGGGGGCAGCC.CC.CCCGGCTTTTCTCAGATCAGCTTg                                                                               <  2:1733909/100‑1 (MQ=255)
                cAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTg                                                                               >  1:3447050/1‑101 (MQ=255)
                 aGTGCCTTTTTCTCAGTTCCTTGAGTGCGCGGCTTTTTTCTGGGCCGAAATACGCCGGGGGT.CGGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGc                                                                              <  2:3055588/101‑1 (MQ=25)
                 aGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGc                                                                              >  1:2435263/1‑101 (MQ=255)
                 aGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGc                                                                              >  2:2418263/1‑101 (MQ=255)
                  gTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGcc                                                                             <  2:1577745/101‑1 (MQ=255)
                    gCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCa                                                                           >  2:6060459/1‑101 (MQ=255)
                     ccATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCAt                                                                          <  1:1606460/101‑1 (MQ=255)
                       tttttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATCCGCCGGGGGT.CCGGGGGCAGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCg                                                                        <  1:3235769/100‑1 (MQ=37)
                       aTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCg                                                                        >  1:838927/1‑101 (MQ=255)
                       aTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCg                                                                        <  2:5296358/101‑1 (MQ=255)
                         tttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGcc                                                                      >  1:7814192/1‑101 (MQ=255)
                          ttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCa                                                                     <  2:502996/101‑1 (MQ=255)
                           tctcGGTTCCTTGAGTTCGCGGCCTTTTTCTGGGCCGAAATACGCGGGGGGG.CCGGGGGCGCCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    <  1:5991249/101‑1 (MQ=255)
                            ctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcg                                                                   >  2:4131340/1‑101 (MQ=255)
                            ctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcg                                                                   <  1:1355154/101‑1 (MQ=255)
                             gcggTTCCGTGGGTTCGCGCCCTTTTTCGAGGCGGAAATCCGCCGGGGGC.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcgg                                                                  <  1:1699683/98‑1 (MQ=21)
                                gTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcggcgg                                                               >  1:4190643/1‑101 (MQ=255)
                                gTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcggcgg                                                               >  1:7047545/1‑101 (MQ=255)
                                 ttCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGt                                                              >  2:2100152/1‑101 (MQ=255)
                                 ttCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGt                                                              <  2:3370881/101‑1 (MQ=255)
                                  tCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  1:2260658/1‑101 (MQ=255)
                                   ccTTGAGGTCGCGGCCTTTTTCTTGGCCGAAATCCGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTAt                                                            <  2:1267025/101‑1 (MQ=25)
                                     ttGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg                                                          >  1:104310/1‑101 (MQ=255)
                                     ttGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg                                                          >  2:8077225/1‑101 (MQ=255)
                                      taaGTTCGCGCCCTTTTCCTTGGCCGAAATGCGCCGGGGGT.CCGGGGCCATCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         <  2:4746952/99‑1 (MQ=25)
                                      tGAGTTCGCGGCCTTTTTCTTGCCCGAAATGCGCGGGGGGT.CGGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         <  1:4828799/101‑1 (MQ=37)
                                        ggTTCGCGGCTTTTTTCTGGGCCGAAACGCGCGGGGGGC.CGGGGGGCAGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGcc                                                       <  1:7736239/100‑1 (MQ=21)
                                          ttCGCGGCCTTTTTCTTGGCCGAAATCCGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCCCCACGGCGGTATCGGCCAt                                                     <  1:874336/101‑1 (MQ=37)
                                          tccgctccttttttGTTGGCCAAAATCCCCGGGGGGG.CGGGGGCCAGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCAt                                                     <  1:7524943/92‑1 (MQ=16)
                                            cgcgGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGTAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGc                                                   >  2:4448967/1‑101 (MQ=37)
                                            cgcgGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGc                                                   <  1:8584627/101‑1 (MQ=255)
                                            cgcgGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGc                                                   <  1:2858260/101‑1 (MQ=255)
                                             gcgGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg                                                  >  2:2251297/1‑101 (MQ=255)
                                                 ccTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc                                              >  1:2491057/1‑101 (MQ=255)
                                                  cTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             >  2:2664694/1‑101 (MQ=255)
                                                  cTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  2:6523509/101‑1 (MQ=255)
                                                  cTTTTTCTCGGCCGAAATCCGCCTGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  2:7218912/101‑1 (MQ=25)
                                                   tttttCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCga                                            >  1:8899629/1‑101 (MQ=255)
                                                   tttttCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCga                                            <  2:6922185/101‑1 (MQ=255)
                                                    ttttCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           <  2:6265968/101‑1 (MQ=255)
                                                    ttttCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           <  1:6797761/101‑1 (MQ=255)
                                                     tttCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgaga                                          >  1:5214612/1‑101 (MQ=255)
                                                      ttCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         <  1:5438683/101‑1 (MQ=255)
                                                      ttCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         >  1:5388419/1‑101 (MQ=255)
                                                       tCTTGGCCGAAATACGCCGGTGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAg                                        <  2:8279573/101‑1 (MQ=25)
                                                        cTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       >  1:2606832/1‑101 (MQ=255)
                                                         ttGGCCGAAATCCGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcc                                      <  2:2536412/101‑1 (MQ=25)
                                                            gCCGAAATAGGCGGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCca                                   <  2:1046654/101‑1 (MQ=21)
                                                            gCCGAAATACGCCGGGGGT.CTGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCCGTTCGAGAAGCCCca                                   <  1:7242089/101‑1 (MQ=25)
                                                             ccGAAA.ACGCCGGGGGTCCCGGGGGCGCCGCCC.CCGGCG.TTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcat                                  <  1:1805564/101‑1 (MQ=17)
                                                               gAAATGCGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                <  2:2742108/101‑1 (MQ=21)
                                                               gAAATCCGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                <  2:2918766/101‑1 (MQ=21)
                                                               gAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                >  2:6844406/1‑101 (MQ=255)
                                                                aaaTACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGCATCGGCCATGCGGTTCGAGAAGCCCcattca                               >  2:5098334/1‑101 (MQ=25)
                                                                  aTACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             >  2:6706592/1‑101 (MQ=255)
                                                                  aTACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             >  2:5841529/1‑101 (MQ=255)
                                                                   tACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTg                            <  1:8077574/101‑1 (MQ=255)
                                                                   tACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTg                            >  1:8171679/1‑101 (MQ=255)
                                                                   tACGCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTg                            >  1:9194146/1‑101 (MQ=255)
                                                                    aCGCCGGGGTT.CCGGAGGCCGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGt                           <  2:7991000/101‑1 (MQ=18)
                                                                      gCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         <  2:838914/101‑1 (MQ=255)
                                                                      gCCGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         >  2:3190400/1‑101 (MQ=255)
                                                                       ccGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        <  2:8655335/101‑1 (MQ=255)
                                                                       ccGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        <  1:5854244/101‑1 (MQ=255)
                                                                       ccGGGGGG.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        <  1:4526217/101‑1 (MQ=21)
                                                                        cGGGGGT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATa                       <  1:3755996/101‑1 (MQ=255)
                                                                         gggggT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      >  1:3547407/1‑101 (MQ=255)
                                                                          ggggT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     <  2:8540308/101‑1 (MQ=255)
                                                                          ggggT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     <  1:1480541/101‑1 (MQ=255)
                                                                          ggggC.CGGGGGGCCGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     <  1:3101220/101‑1 (MQ=17)
                                                                           gggT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCa                    <  1:3980798/101‑1 (MQ=255)
                                                                           gggC.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCTGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGCTCGAGAAGCCCCATTTATTGTCATACCa                    <  1:6516203/101‑1 (MQ=18)
                                                                            ggT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCAc                   <  1:7344448/101‑1 (MQ=255)
                                                                             gT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  <  2:7047260/101‑1 (MQ=255)
                                                                             gT.CCGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  >  2:5478353/1‑101 (MQ=255)
                                                                                ccGGGGGCAGCGCCC.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGAc                >  2:1375028/1‑101 (MQ=255)
                                                                                 cGGGGGCAGCGCCC.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa               >  2:9117486/1‑101 (MQ=255)
                                                                                 cGGGGGCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa               <  2:396181/101‑1 (MQ=255)
                                                                                     ggCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGAt           >  1:1084074/1‑101 (MQ=255)
                                                                                     ggCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGAt           <  2:2470611/101‑1 (MQ=255)
                                                                                     ggCAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGAt           <  1:6372054/101‑1 (MQ=255)
                                                                                       cAGCG.CCGCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCCTGCGGTTCGAGAAGACCCATTCATTGTCCTACCACGACAGGATGc         >  2:2645980/1‑101 (MQ=17)
                                                                                       cAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         <  2:8865029/101‑1 (MQ=255)
                                                                                         gcg.cC.CCCGGCTTTTCTCAGATCAGCTTGCCGATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCgg       <  1:6586996/101‑1 (MQ=18)
                                                                                         gcg.cC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCgg       >  2:1847852/1‑101 (MQ=255)
                                                                                            ccc.ccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    <  2:3704995/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
GTTGCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGT.CCGGGGGCAGCG.CC.CCGGCGTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976797‑1976990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG