Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,990,962 T→A 100% Y555F (TAC→TTC)  glgB ← 1,4‑alpha‑glucan branching protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,990,9620T→A100.0% 2159.6 541Y555F (TAC→TTC) glgB1,4‑alpha‑glucan branching protein
Reads supporting (aligned to +/- strand):  new base (263/278):  ref base (0/0):  total (263/278)

Only 100 of 546 total aligned reads displayed.

GAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGTACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACT  >  NC_014034/1990864‑1991059
                                                                                                  |                                                                                                  
gAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAAc                                                                                                  <  1:911811/101‑1 (MQ=255)
 aaGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACg                                                                                                 >  1:7043712/1‑101 (MQ=255)
  aGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGc                                                                                                <  2:318250/101‑1 (MQ=255)
  aGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGc                                                                                                >  1:4790666/1‑101 (MQ=255)
   ggTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCa                                                                                               >  2:1380671/1‑101 (MQ=255)
   ggTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCa                                                                                               >  1:2292375/1‑101 (MQ=255)
    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCAt                                                                                              >  2:4504880/1‑101 (MQ=255)
    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCAt                                                                                              <  2:3724549/101‑1 (MQ=255)
     tGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATa                                                                                             <  2:8748238/101‑1 (MQ=255)
     tGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATa                                                                                             <  2:8374883/101‑1 (MQ=255)
         aGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAg                                                                                         <  1:2002380/101‑1 (MQ=255)
          gTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAgg                                                                                        >  1:895014/1‑101 (MQ=255)
            cAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGcc                                                                                      >  2:8904296/1‑101 (MQ=255)
             aaGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGccc                                                                                     <  1:1729475/101‑1 (MQ=255)
              aGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCg                                                                                    >  2:7920954/1‑101 (MQ=255)
              aGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCg                                                                                    <  2:1867570/101‑1 (MQ=255)
               gggCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGc                                                                                   <  1:8497455/101‑1 (MQ=255)
                ggCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCa                                                                                  <  1:8027977/101‑1 (MQ=255)
                 gCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAg                                                                                 <  2:2637044/101‑1 (MQ=255)
                     ccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTg                                                                             >  2:1964239/1‑101 (MQ=255)
                     ccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTg                                                                             >  1:79327/1‑101 (MQ=255)
                     ccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTg                                                                             >  1:5899181/1‑101 (MQ=255)
                      cTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTgg                                                                            <  1:7177862/101‑1 (MQ=255)
                        gATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCg                                                                          >  2:4218583/1‑101 (MQ=255)
                        gATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCg                                                                          >  2:7368663/1‑101 (MQ=255)
                        gATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCg                                                                          <  1:5034004/101‑1 (MQ=255)
                        gATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCg                                                                          <  1:120035/101‑1 (MQ=255)
                         aTGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCGTTGGAACGCATAGAAGGCCCGCAGGTTGGCGa                                                                         >  2:6750268/1‑101 (MQ=255)
                          tGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGaa                                                                        >  2:3585976/1‑101 (MQ=255)
                          tGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGaa                                                                        <  1:7617295/101‑1 (MQ=255)
                          tGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGaa                                                                        >  1:1024252/1‑101 (MQ=255)
                           gcgcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAt                                                                       >  1:1525569/1‑101 (MQ=255)
                           gcgcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAt                                                                       >  1:6892383/1‑101 (MQ=255)
                             gcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAttt                                                                     >  1:8993439/1‑101 (MQ=255)
                             gcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAttt                                                                     <  2:2902163/101‑1 (MQ=255)
                             gcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAttt                                                                     >  1:8347578/1‑101 (MQ=255)
                              cccATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTc                                                                    <  1:960302/101‑1 (MQ=255)
                              cccATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTAGCCCGGCTGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTc                                                                    >  1:4950081/1‑101 (MQ=255)
                               ccATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCt                                                                   <  2:3902288/101‑1 (MQ=255)
                                 aTTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGc                                                                 >  2:227015/1‑101 (MQ=255)
                                  ttCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCa                                                                <  2:6317988/101‑1 (MQ=255)
                                   tCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCaa                                                               <  1:3561870/101‑1 (MQ=255)
                                    cgcgCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAAc                                                              <  1:3749707/101‑1 (MQ=255)
                                     gcgcCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAAcc                                                             <  1:8417275/101‑1 (MQ=255)
                                      cgcCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAAccc                                                            <  1:8714254/101‑1 (MQ=255)
                                       gcCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCg                                                           <  2:818000/101‑1 (MQ=255)
                                           cTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGtc.tc                                                      <  1:5588915/101‑1 (MQ=255)
                                              cgcgAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCg                                                   <  2:2449251/101‑1 (MQ=255)
                                                cgAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGc                                                 >  1:4483183/1‑101 (MQ=255)
                                                 gAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTTCCCGGc                                                 >  2:5817899/1‑101 (MQ=255)
                                                 gAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCa                                                >  2:40712/1‑101 (MQ=255)
                                                 gAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCa                                                <  1:2709034/101‑1 (MQ=255)
                                                 gAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCa                                                >  2:5595509/1‑101 (MQ=255)
                                                   aCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATc                                              >  1:6060654/1‑101 (MQ=255)
                                                   aCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATc                                              <  1:3593296/101‑1 (MQ=255)
                                                     tCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCtt                                            <  1:3129568/101‑1 (MQ=255)
                                                     gccTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCtt                                            <  2:9003387/100‑1 (MQ=255)
                                                      ccTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTg                                           <  2:5297102/101‑1 (MQ=255)
                                                       cTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGGGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGc                                          >  2:8624003/1‑101 (MQ=255)
                                                        tGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGcc                                         >  1:4051525/1‑101 (MQ=255)
                                                         gCCCCATGAAGAGCAGCTTCTTGCCAGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGccc                                        <  1:7793658/101‑1 (MQ=255)
                                                          ccccATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCa                                       <  2:1470525/101‑1 (MQ=255)
                                                          ccccATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCa                                       >  1:2478320/1‑101 (MQ=255)
                                                           cccATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAg                                      >  1:4172278/1‑101 (MQ=255)
                                                             cATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCa                                    <  2:4714617/101‑1 (MQ=255)
                                                             cATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCa                                    >  2:4842118/1‑101 (MQ=255)
                                                              aTGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCAt                                   <  1:6881894/101‑1 (MQ=255)
                                                               ggAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATc                                  <  1:2942126/100‑1 (MQ=255)
                                                                gAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCg                                 <  2:4381112/101‑1 (MQ=255)
                                                                gAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCg                                 >  2:3334567/1‑101 (MQ=255)
                                                                gAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCg                                 <  1:5278804/101‑1 (MQ=255)
                                                                gAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCg                                 <  1:1379571/101‑1 (MQ=255)
                                                                  agagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGAc                               >  2:55272/1‑101 (MQ=255)
                                                                  agagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGAc                               >  1:4692407/1‑101 (MQ=255)
                                                                  agagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGAc                               <  2:3096719/101‑1 (MQ=255)
                                                                     gcagcTTCTTCCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGAcccc                            <  1:7364560/101‑1 (MQ=255)
                                                                      cagcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCt                           <  1:6741417/101‑1 (MQ=255)
                                                                       agcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCtt                          <  2:5115003/101‑1 (MQ=255)
                                                                       agcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCtt                          >  2:6870183/1‑101 (MQ=255)
                                                                           tcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCg                      >  2:641133/1‑101 (MQ=255)
                                                                           tcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCg                      >  1:1806291/1‑101 (MQ=255)
                                                                             ttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTg                    >  1:6343416/1‑101 (MQ=255)
                                                                               gCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGaa                  <  2:6985480/101‑1 (MQ=255)
                                                                               gCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGaa                  >  2:709484/1‑101 (MQ=255)
                                                                               gCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGaa                  >  2:7647945/1‑101 (MQ=255)
                                                                               gCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGaa                  <  1:5772729/101‑1 (MQ=255)
                                                                               gCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGaa                  >  2:2032866/1‑101 (MQ=255)
                                                                                gccGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAAc                 <  1:652523/100‑1 (MQ=255)
                                                                                cccGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAAc                 >  1:3381412/1‑101 (MQ=255)
                                                                                cccGGATGCCCCCATTGGAACGCATAGAAGGCCCGCGGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGagc                 <  2:7281055/101‑3 (MQ=255)
                                                                                 ccGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACg                <  1:4627355/101‑1 (MQ=255)
                                                                                   ggATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGAc              >  1:1648103/1‑101 (MQ=255)
                                                                                   ggATGCACCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGGCCCCTTGCCGTGAACGAc              <  2:3503224/101‑1 (MQ=255)
                                                                                     gtGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCt            <  2:1147898/100‑1 (MQ=255)
                                                                                     aTGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCt            >  1:5399457/1‑101 (MQ=255)
                                                                                       gcgcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcg          >  1:3849897/1‑101 (MQ=255)
                                                                                            cATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTg     <  1:2091499/101‑1 (MQ=255)
                                                                                             aTTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGa    >  1:3471501/1‑101 (MQ=255)
                                                                                              ttGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGAc   <  2:4301514/101‑1 (MQ=255)
                                                                                              ttGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGAc   <  2:1676968/101‑1 (MQ=255)
                                                                                                  |                                                                                                  
GAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGTACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTC.TCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACT  >  NC_014034/1990864‑1991059

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG