Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,040,375 C→G 2.3% intergenic (‑123/‑265) RCAP_rcc01888 ← / → RCAP_rcc01889 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,040,3750C→G 2.3% 12.4 ‑0.3 ‑0.1428intergenic (‑123/‑265)RCAP_rcc01888/RCAP_rcc01889hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/5):  ref base (235/183):  total (240/188)
Fisher's exact test strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.13e-01

Only 100 of 473 total aligned reads displayed.

CCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGG.CGCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAAATC  >  NC_014034/2040276‑2040476
                                                                                                   |                                                                                                      
    ccTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCgg                                                                                                 <  1:218764/101‑1 (MQ=255)
     ctctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGa                                                                                                >  2:5657971/1‑101 (MQ=255)
     ctctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGa                                                                                                >  2:8853061/1‑101 (MQ=255)
     ctctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGa                                                                                                >  2:7765528/1‑101 (MQ=255)
       ctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATg                                                                                              <  2:3950863/101‑1 (MQ=255)
       ctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATg                                                                                              >  1:4038600/1‑101 (MQ=255)
       ctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATg                                                                                              >  2:7345479/1‑101 (MQ=255)
         gggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGc                                                                                            >  1:7054212/1‑101 (MQ=255)
           gTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCtt                                                                                          <  1:2448318/101‑1 (MQ=255)
           gTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCtt                                                                                          <  2:717614/101‑1 (MQ=255)
            ttGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTa                                                                                         <  1:1262458/101‑1 (MQ=255)
             tggaagggAAAAAGAGCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                        >  1:8836821/7‑101 (MQ=255)
             tGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                        >  1:6905358/1‑101 (MQ=255)
             tGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                        <  2:2763366/101‑1 (MQ=255)
                gATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcgcg                                                                                     >  2:1180911/1‑101 (MQ=255)
                 aTGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCgg                                                                                    >  1:4983387/1‑101 (MQ=255)
                 aTGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCgg                                                                                    >  2:5479231/1‑101 (MQ=255)
                    gAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTAt                                                                                 <  2:8571518/101‑1 (MQ=255)
                    gAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTAt                                                                                 <  1:743797/101‑1 (MQ=255)
                    gAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTAt                                                                                 >  1:6323008/1‑101 (MQ=255)
                     aaaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATg                                                                                >  1:4222989/1‑101 (MQ=255)
                     aaaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATg                                                                                >  1:1322577/1‑101 (MQ=255)
                     aaaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATg                                                                                <  1:932958/101‑1 (MQ=255)
                     aaaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATg                                                                                >  1:9161530/1‑101 (MQ=255)
                      aaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATgg                                                                               <  2:6174663/101‑1 (MQ=255)
                       ttccaatctaaCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGgc                                                                              <  1:8592305/92‑1 (MQ=255)
                          ggccGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGcgcg                                                                           <  1:2970398/99‑1 (MQ=255)
                            ccGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTg                                                                         >  2:7925377/1‑101 (MQ=255)
                            ccGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTg                                                                         >  2:7586383/1‑101 (MQ=255)
                              ggAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGcc                                                                       >  2:4849735/1‑101 (MQ=255)
                              ggAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGcc                                                                       <  1:6058674/101‑1 (MQ=255)
                                 aCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCaaa                                                                    <  1:5749267/101‑1 (MQ=255)
                                  cGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAg                                                                   <  1:3859519/101‑1 (MQ=255)
                                   ggCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGa                                                                  >  1:4873748/1‑101 (MQ=255)
                                     ccAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcg                                                                <  1:5646297/101‑1 (MQ=255)
                                     ccAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcg                                                                >  1:4483231/1‑101 (MQ=255)
                                     ccAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcg                                                                <  1:776565/101‑1 (MQ=255)
                                     ccAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcg                                                                <  2:6409238/101‑1 (MQ=255)
                                      cAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcgg                                                               >  2:8690341/1‑101 (MQ=255)
                                        ggggCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGGATGGCGCGGGCCGAAGAcggtg                                                             >  2:2071049/1‑99 (MQ=255)
                                          ggCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACggcggc                                                           <  1:5179208/101‑1 (MQ=255)
                                           gCCAGACGCAGGCGT.GGGGGTTCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGATGGCGGcc                                                          <  2:9077240/101‑1 (MQ=255)
                                           gCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGcc                                                          >  1:7881225/1‑101 (MQ=255)
                                           gCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGcc                                                          <  1:2618425/101‑1 (MQ=255)
                                             cAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAGAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGc                                                        >  2:6254149/1‑101 (MQ=255)
                                             cAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGc                                                        >  2:2595988/1‑101 (MQ=255)
                                             cAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGc                                                        >  1:4340361/1‑101 (MQ=255)
                                              aGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCa                                                       >  2:581215/1‑101 (MQ=255)
                                                aCGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCaaa                                                     >  1:1000124/1‑101 (MQ=255)
                                                 cGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAg                                                    <  2:1666437/101‑1 (MQ=255)
                                                  gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGATGGCGGCCGCAAAgg                                                   >  1:4703088/1‑101 (MQ=255)
                                                  gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAgg                                                   >  1:8758194/1‑101 (MQ=255)
                                                  gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAgg                                                   >  1:8985656/1‑101 (MQ=255)
                                                     ggCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.ccg                                               <  1:2268852/101‑3 (MQ=255)
                                                       gcgtgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.ccgc                                              <  2:7017088/97‑4 (MQ=255)
                                                           gggggATCCGGTGGAAGGTCAGACGTTGTGCAAAAGC.GCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCa                                           >  1:6578658/1‑101 (MQ=255)
                                                           gggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCa                                           <  2:4321900/101‑1 (MQ=255)
                                                             gggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATa                                         >  1:6491448/1‑101 (MQ=255)
                                                              ggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATaa                                        >  2:4961679/1‑101 (MQ=255)
                                                                 tCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTg                                     <  1:5471638/101‑1 (MQ=255)
                                                                 tCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTg                                     <  2:4644381/101‑1 (MQ=255)
                                                                    ggTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGc                                  >  2:4136958/1‑101 (MQ=255)
                                                                     gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGcc                                 <  1:3626266/101‑1 (MQ=255)
                                                                      tCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGccc                                <  2:5373727/101‑1 (MQ=255)
                                                                       cGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAAGGCCGCGCCATAAGTGAGCCCa                               >  1:5048368/1‑101 (MQ=255)
                                                                        gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAc                              >  1:1854176/1‑101 (MQ=255)
                                                                         aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAATGGCCGCGCCATAAGTGAGCCCAcc                             <  2:4379584/101‑1 (MQ=255)
                                                                         aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAcc                             >  2:6872149/1‑101 (MQ=255)
                                                                         aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAcc                             <  1:8426174/101‑1 (MQ=255)
                                                                         aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAcc                             >  2:3455697/1‑101 (MQ=255)
                                                                         aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAcc                             >  2:3558002/1‑101 (MQ=255)
                                                                         aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAcc                             >  2:8259353/1‑101 (MQ=255)
                                                                         aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAcc                             >  1:5822594/1‑101 (MQ=255)
                                                                             tCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAccggtt                         >  1:6793742/1‑97 (MQ=255)
                                                                              cAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTc                       >  1:3469890/1‑101 (MQ=255)
                                                                              cAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTc                       >  1:8319726/1‑101 (MQ=255)
                                                                              cAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTc                       >  2:1492121/1‑101 (MQ=255)
                                                                               aGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCg                      <  1:3305017/101‑1 (MQ=255)
                                                                               aGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCg                      <  2:296194/101‑1 (MQ=255)
                                                                                gACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCgg                     <  1:1596802/101‑1 (MQ=255)
                                                                                 aCGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGa                    >  2:3224352/1‑101 (MQ=255)
                                                                                     tgtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt                >  2:2330523/1‑99 (MQ=255)
                                                                                      gtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggttg               <  2:6731057/101‑4 (MQ=255)
                                                                                       tgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggttgg              <  1:725812/101‑5 (MQ=255)
                                                                                         cAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGAt           <  2:5198119/101‑1 (MQ=255)
                                                                                         cAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGAt           <  1:6242076/101‑1 (MQ=255)
                                                                                           aaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGc         >  2:2458042/1‑101 (MQ=255)
                                                                                            aaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCa        >  1:2869117/1‑101 (MQ=255)
                                                                                               gcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATa      <  2:5037929/98‑1 (MQ=255)
                                                                                               gcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATa      <  2:8369859/98‑1 (MQ=255)
                                                                                               gcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATa      <  1:2068698/98‑1 (MQ=255)
                                                                                               gcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATa      <  2:3765644/98‑1 (MQ=255)
                                                                                               gcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATa      <  1:5385700/98‑1 (MQ=255)
                                                                                               gcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATa      <  2:6137370/98‑1 (MQ=255)
                                                                                                caccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaa     <  2:1322711/99‑1 (MQ=255)
                                                                                                caccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaa     >  2:8068465/3‑101 (MQ=255)
                                                                                                caccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCC.GCCGGTTCGGA.GGTTGGATGCATaa     >  2:2894902/3‑101 (MQ=255)
                                                                                                 accGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaaa    <  1:52973/100‑1 (MQ=255)
                                                                                                  ccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAAt   <  1:1540020/101‑1 (MQ=255)
                                                                                                   cGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATc  >  1:7996979/1‑101 (MQ=255)
                                                                                                   |                                                                                                      
CCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGG.CGCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAAATC  >  NC_014034/2040276‑2040476

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG