BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,057,834	A→C	15.0%	intergenic (‑600/‑302)	RCAP_rcc01900 ← / → RCAP_rcc01901	hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,057,834	0	A→C	15.0%	137.9 ‑0.3 ‑1.4	340	intergenic (‑600/‑302)	RCAP_rcc01900/RCAP_rcc01901	hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand): new base (28/23): ref base (151/138): total (179/161)
Fisher's exact test strand distribution p-value = 7.63e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.86e-02

Only 100 of 353 total aligned reads displayed.


CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCG.CG.CG.C.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913


 tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGca                                                                                <  1:4171429/101‑2 (MQ=255)


 tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGca                                                                                >  2:5921739/1‑100 (MQ=255)


  gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGTGACAAT.CC..CG.CG.C.CGcaa                                                                               <  2:6485872/101‑3 (MQ=255)


  gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaa                                                                               >  2:3588780/1‑99 (MQ=255)


   tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaag                                                                              >  2:6737064/1‑98 (MQ=255)


   tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaag                                                                              <  1:8412165/101‑4 (MQ=255)


    ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaagc                                                                             <  2:4186327/101‑5 (MQ=255)


    ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaagc                                                                             >  2:2347261/1‑97 (MQ=255)


     tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaagcg                                                                            >  2:4665779/1‑96 (MQ=255)


       ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcacgcgtt                                                                          <  2:4521733/101‑8 (MQ=255)


        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaagcgttt                                                                         <  2:3392961/101‑9 (MQ=255)


        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaagcgttt                                                                         <  2:8330394/101‑9 (MQ=255)


        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaagcgttt                                                                         >  1:6928750/1‑93 (MQ=255)


          cccATTACCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaagcgtttcc                                                                       <  2:4939302/101‑11 (MQ=255)


          cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaagcgtttcc                                                                       >  1:4972289/1‑91 (MQ=255)


          cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CG.C.CGcaagcgtttcc                                                                       >  2:8872182/1‑91 (MQ=255)


                accccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCg                                                                     <  2:2221173/99‑1 (MQ=255)


                aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCg                                                                     >  1:4773467/1‑101 (MQ=255)


                aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCg                                                                     >  1:4932228/1‑101 (MQ=255)


                 aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCgg                                                                    <  1:6810022/101‑1 (MQ=255)


                 aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCgg                                                                    >  1:8719876/1‑101 (MQ=255)


                  cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATAGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGc                                                                   >  1:6690509/1‑101 (MQ=255)


                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCAt                                                                 <  1:7524580/101‑1 (MQ=255)


                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCAt                                                                 >  1:1955119/1‑101 (MQ=255)


                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATACCG.CGCCG.CAAGCGTTTCCGAACCGGCAt                                                                 <  1:2507464/101‑1 (MQ=255)


                     tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATc                                                                <  2:5679808/101‑1 (MQ=255)


                     tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATc                                                                >  1:3275808/1‑101 (MQ=255)


                      gCTTCGGCGCGCGGATTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCa                                                               >  1:8032746/1‑101 (MQ=255)


                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGAGTTTCCGGATTGGCGACAATCCCG.CGCCGCC.AGCGTTTCCGAACCGGCATct                                                               >  1:1311709/1‑100 (MQ=255)


                       cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCat                                                              <  1:5985523/101‑2 (MQ=255)


                       cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCat                                                              >  2:1120411/1‑100 (MQ=255)


                       cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCat                                                              <  1:2017661/101‑2 (MQ=255)


                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCatt                                                             <  1:5801712/101‑3 (MQ=255)


                         tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTc                                                            <  1:6484782/101‑1 (MQ=255)


                         tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTc                                                            >  1:7319861/1‑101 (MQ=255)


                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCt                                                           >  2:2108929/1‑101 (MQ=255)


                           ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCtt                                                          <  2:2501539/101‑1 (MQ=255)


                            gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTg                                                         >  2:1254713/1‑101 (MQ=255)


                              gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGG.c                                                      <  1:2995960/101‑1 (MQ=255)


                              gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGG.c                                                      <  1:144650/101‑1 (MQ=255)


                               cgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGG.cc                                                     <  2:7999524/101‑1 (MQ=255)


                                  ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  >  1:7084681/1‑101 (MQ=255)


                                  ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  >  1:5435016/1‑101 (MQ=255)


                                  ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCATGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  >  1:8017479/1‑101 (MQ=255)


                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 >  2:6041826/1‑100 (MQ=255)


                                    aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccg                                                >  1:5333341/1‑99 (MQ=255)


                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              >  1:7774770/1‑97 (MQ=255)


                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              <  1:6043626/101‑5 (MQ=255)


                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              >  2:803833/1‑97 (MQ=255)


                                        cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGACG.CAAGCGTTTCCGCACCGGCAGCATTCTTGG.ccccccgctgg                                            >  2:9082242/1‑95 (MQ=255)


                                         ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCggg                                            <  2:8553530/101‑1 (MQ=255)


                                          gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGa                                           >  1:1031047/1‑101 (MQ=255)


                                              ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                       >  1:2167491/1‑101 (MQ=255)


                                               gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATc                                      <  2:7468074/101‑1 (MQ=255)


                                                cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     <  1:8888483/101‑1 (MQ=255)


                                                cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     <  1:7259580/101‑1 (MQ=255)


                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    <  2:5319922/101‑1 (MQ=255)


                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    >  2:3968091/1‑101 (MQ=255)


                                                   cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgg                                  >  2:4874778/1‑101 (MQ=255)


                                                   cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgg                                  >  1:1844185/1‑101 (MQ=255)


                                                    ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 >  1:4041844/1‑101 (MQ=255)


                                                     gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgcc                                >  2:7128178/1‑101 (MQ=255)


                                                      ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               >  2:7307775/1‑101 (MQ=255)


                                                      ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               >  2:2710626/1‑101 (MQ=255)


                                                        ctctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccgcc                             <  2:786212/101‑1 (MQ=255)


                                                         tctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  2:2004910/1‑101 (MQ=255)


                                                         tctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  2:4322357/1‑101 (MQ=255)


                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCTGCCTc                           >  1:3384351/1‑101 (MQ=255)


                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           <  2:6394661/101‑1 (MQ=255)


                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           <  2:5532764/101‑1 (MQ=255)


                                                           ttGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          <  1:4375499/101‑1 (MQ=255)


                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         <  1:7682987/101‑1 (MQ=255)


                                                             gCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAtt                        >  1:1768596/1‑101 (MQ=255)


                                                             gCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAtt                        <  2:876785/101‑1 (MQ=255)


                                                             gCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAtt                        >  2:6878622/1‑101 (MQ=255)


                                                             gCGATCCTGCGTTTCCGGATTGGCGACAATCCCA.CGCCG.CAAGCGTTTCCGAACCGACATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAtt                        <  1:9082664/101‑1 (MQ=255)


                                                              cGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       <  1:2323411/101‑1 (MQ=255)


                                                              cGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       >  1:521252/1‑101 (MQ=255)


                                                               gATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      >  2:1464196/1‑101 (MQ=255)


                                                                aTCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     >  2:5190077/1‑101 (MQ=255)


                                                                aTCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     <  1:8196535/101‑1 (MQ=255)


                                                                aTCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     <  2:1693681/101‑1 (MQ=255)


                                                                 tCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    <  2:3742709/101‑1 (MQ=255)


                                                                  ccTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   >  1:4531972/1‑101 (MQ=255)


                                                                  ccTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   <  1:5045951/101‑1 (MQ=255)


                                                                   cTGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  >  2:9184915/1‑101 (MQ=255)


                                                                    tGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 >  1:1225308/1‑101 (MQ=255)


                                                                    tGCGTTTCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 >  2:1071994/1‑101 (MQ=255)


                                                                        tttCCGGATTGGCGACAATCCCGCCC.CG.CAAGCGTTTCCGACCCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  2:3195316/101‑1 (MQ=255)


                                                                        tttCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  1:361867/101‑1 (MQ=255)


                                                                        tttCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             >  2:90924/1‑101 (MQ=255)


                                                                         ttCCGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            >  1:1072597/1‑101 (MQ=255)


                                                                           ccGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          >  1:470338/1‑101 (MQ=255)


                                                                            cGGATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAGTGCGa         >  1:2840758/1‑101 (MQ=255)


                                                                              gATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       <  1:2189314/101‑1 (MQ=255)


                                                                              gATTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       >  1:8622832/1‑101 (MQ=255)


                                                                               aTTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  2:2859206/101‑1 (MQ=255)


                                                                               aTTGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  1:9184902/1‑101 (MQ=255)


                                                                                ttGGCGACAATCCCG.CGCCG.CAAGCGTTTCCGACCCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  2:763112/101‑1 (MQ=255)


                                                                                   gCGACAATCCCG.CGCCG.CAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  <  1:1634537/101‑1 (MQ=255)


CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCG.CG.CG.C.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913

Base quality scores:


ATCG

< 3 ≤


ATCG

< 22 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 40 ≤


ATCG