BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,123,404	A→G	2.1%	P256P (CCT→CCC)	wza ←	polysaccharide export protein Wza

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,123,404	0	A→G	2.1%	10.1 0.0 ‑0.0	416	P256P (CCT→CCC)	wza	polysaccharide export protein Wza
Reads supporting (aligned to +/- strand): new base (4/5): ref base (199/207): total (204/212)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.30e-01

Only 100 of 451 total aligned reads displayed.


AAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATG  >  NC_014034/2123306‑2123503


aaGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGgg                                                                                                    >  2:5956268/1‑101 (MQ=255)


 aGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGggc                                                                                                   <  1:3910660/101‑2 (MQ=255)


 aGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGggc                                                                                                   <  1:1330672/101‑2 (MQ=255)


   aTGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCtt                                                                                                >  2:7456268/1‑101 (MQ=255)


    tGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTg                                                                                               <  1:8709187/101‑1 (MQ=255)


        gcgGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcga                                                                                           >  2:4721378/1‑99 (MQ=255)


         cgGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgat                                                                                          >  1:2513639/1‑98 (MQ=255)


          gggCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatc                                                                                         >  1:1979465/1‑97 (MQ=255)


           ggCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatct                                                                                        >  1:4377310/1‑96 (MQ=255)


            gCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctg                                                                                       <  2:8020240/101‑7 (MQ=255)


             cATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctgg                                                                                      >  1:1217130/1‑94 (MQ=255)


                cGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggccc                                                                                   <  1:8234872/101‑11 (MQ=255)


                cGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggccc                                                                                   <  1:3057982/101‑11 (MQ=255)


                         ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCATGGTTATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAg                                                                          <  2:2697144/101‑1 (MQ=255)


                         ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAg                                                                          <  2:6854631/101‑1 (MQ=255)


                         ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAg                                                                          <  1:7261230/101‑1 (MQ=255)


                           tCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGc                                                                        <  1:8474786/101‑1 (MQ=255)


                           tCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGc                                                                        <  2:4608406/101‑1 (MQ=255)


                           gcGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGc                                                                        <  2:8819903/100‑1 (MQ=255)


                            cGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcc                                                                       <  1:4990959/101‑1 (MQ=255)


                               ccAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcctcc                                                                    >  2:3648005/1‑101 (MQ=255)


                               ccAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcctcc                                                                    <  1:3688402/101‑1 (MQ=255)


                               ccACACCCCCGACGCGGGTTACCGCCCTGGTCAGCGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcctcc                                                                    <  2:1351444/101‑1 (MQ=255)


                                 aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTg                                                                  <  1:7456592/101‑1 (MQ=255)


                                  gACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGc                                                                 <  1:3152407/101‑1 (MQ=255)


                                  gACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTAATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGc                                                                 <  2:6565687/101‑1 (MQ=255)


                                    ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                               <  2:151618/101‑1 (MQ=255)


                                    ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                               <  2:6352984/101‑1 (MQ=255)


                                     cgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                              <  1:5434945/101‑1 (MQ=255)


                                        cgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTc                                                           >  2:1006388/1‑101 (MQ=255)


                                         gACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCt                                                          >  2:752957/1‑101 (MQ=255)


                                         gACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCt                                                          >  2:7312256/1‑101 (MQ=255)


                                          aCGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTg                                                         <  2:3300315/101‑1 (MQ=255)


                                           cgcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgc                                                        <  1:1120784/101‑1 (MQ=255)


                                            gcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgcc                                                       <  1:8007863/101‑1 (MQ=255)


                                             cgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccg                                                      >  2:8529388/1‑101 (MQ=255)


                                             cgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccg                                                      >  2:1891333/1‑101 (MQ=255)


                                             cgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccg                                                      >  1:2407419/1‑101 (MQ=255)


                                             cgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccg                                                      <  2:7019763/101‑1 (MQ=255)


                                                gTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                                   <  1:4680483/101‑1 (MQ=255)


                                                gTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                                   >  1:485279/1‑101 (MQ=255)


                                                  gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                 >  2:6686796/1‑101 (MQ=255)


                                                  gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                 >  2:5624557/1‑101 (MQ=255)


                                                  gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                 >  2:16479/1‑101 (MQ=255)


                                                   aTCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGT.GGAGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCGGGACCGTCTGCCGCCCGAc                                                >  1:2875978/1‑101 (MQ=255)


                                                    tCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACg                                               <  2:5301291/101‑1 (MQ=255)


                                                      gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                             <  2:4918027/101‑1 (MQ=255)


                                                      gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                             <  1:7144716/101‑1 (MQ=255)


                                                      gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                             <  2:1979405/101‑1 (MQ=255)


                                                         tGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgac                                          >  1:2691776/1‑101 (MQ=255)


                                                         tGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgac                                          >  1:7731140/1‑101 (MQ=255)


                                                         tGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgac                                          >  1:1869148/1‑101 (MQ=255)


                                                          gggTTAGTGTTATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                         <  1:33490/101‑1 (MQ=255)


                                                          gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                         <  2:1269623/101‑1 (MQ=255)


                                                          gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                         <  1:6218680/101‑1 (MQ=255)


                                                            gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                       <  1:6105722/101‑1 (MQ=255)


                                                               aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                    >  1:2317170/1‑101 (MQ=255)


                                                                gggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCg                                   <  2:5184940/101‑1 (MQ=255)


                                                                gggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCg                                   >  2:8292521/1‑101 (MQ=255)


                                                                gggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCg                                   >  1:6661117/1‑101 (MQ=255)


                                                                 ggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGc                                  >  2:2578210/1‑101 (MQ=255)


                                                                 ggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGc                                  >  1:2588091/1‑101 (MQ=255)


                                                                  gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                 >  2:2418369/1‑101 (MQ=255)


                                                                       gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                            <  2:7141940/101‑1 (MQ=255)


                                                                        ttttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTg                           <  2:624899/101‑1 (MQ=255)


                                                                         tttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgc                          >  1:6447998/1‑101 (MQ=255)


                                                                          ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                         <  1:8602549/101‑1 (MQ=255)


                                                                          ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                         >  1:6331375/1‑101 (MQ=255)


                                                                          ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                         >  2:5046381/1‑101 (MQ=255)


                                                                            ccAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                       <  1:7837299/101‑1 (MQ=255)


                                                                            ccAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                       >  2:7909326/1‑101 (MQ=255)


                                                                              aGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAc                     <  1:4585827/101‑1 (MQ=255)


                                                                              aGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAc                     >  1:6680299/1‑101 (MQ=255)


                                                                                cGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCg                   <  1:8827732/101‑1 (MQ=255)


                                                                                 gTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCGGTTTTGCAGCACCgg                  >  1:4235538/1‑101 (MQ=255)


                                                                                 gTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgg                  >  2:6139824/1‑101 (MQ=255)


                                                                                 gTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgg                  <  2:2290397/101‑1 (MQ=255)


                                                                                   cAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt                >  1:9023632/1‑101 (MQ=255)


                                                                                   cAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt                >  2:5728243/1‑101 (MQ=255)


                                                                                   cAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt                >  1:95116/1‑101 (MQ=255)


                                                                                    aGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt               <  1:1429448/101‑1 (MQ=255)


                                                                                    aGATCGACCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt               <  1:5959969/101‑1 (MQ=255)


                                                                                     gatcgatcGTCG.CGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg              >  1:8089686/1‑101 (MQ=255)


                                                                                     gatcgatcGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGCCATCGCCGTTTTGCAGCACCGGgttg              >  2:2882763/1‑101 (MQ=255)


                                                                                     gatcgatcGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg              >  1:168097/1‑101 (MQ=255)


                                                                                        cgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg           <  1:9114813/101‑1 (MQ=255)


                                                                                        cgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg           >  1:2171729/1‑101 (MQ=255)


                                                                                        cgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACACCGCCGTTTTGCAGCACCGGGttggtg           >  2:2944324/1‑101 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg          >  2:8408468/1‑101 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg          <  2:8682795/101‑1 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg          <  1:1006406/101‑1 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg          >  1:5714994/1‑101 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg          >  2:3209296/1‑101 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg          <  2:4051609/101‑1 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGAAGCACCGGGTTGTTgg          >  2:3959060/1‑101 (MQ=255)


                                                                                          atcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgc         <  1:7012942/101‑1 (MQ=255)


                                                                                          atcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgc         <  2:3518281/101‑1 (MQ=255)


                                                                                              gtcgaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGCCGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGcc      >  1:7357988/5‑101 (MQ=255)


                                                                                               tcgaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCg     <  1:5206141/98‑1 (MQ=255)


                                                                                                 gaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt   >  1:7502552/2‑101 (MQ=255)


AAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATG  >  NC_014034/2123306‑2123503

Base quality scores:


ATCG

< 3 ≤


ATCG

< 25 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG