Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,440 G→C 15.5% S2T (AGC→ACC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4400G→C15.5% 141.6 ‑0.9 ‑0.0367S2T (AGC→ACC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (24/33):  ref base (151/159):  total (175/192)
Fisher's exact test strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 386 total aligned reads displayed.

CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACC  >  NC_014034/2155340‑2155540
                                                                                                      |                                                                                                    
ccgccgACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAg                                                                                                      >  2:5767958/1‑101 (MQ=255)
 ggccgGCGCGGCGGCGGTCCCCCCCACCTGCGGATGCCCGG.GGGCGCCGCCTCCGTCACCGCCC.GCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGc                                                                                                     <  1:7405216/100‑1 (MQ=255)
 ccccgCCCCGGCGGCGCTCGCCCTCACCTTCTGATGCCCTG.GGGCCCCGTCACCGTCCCCGCCC.GCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGc                                                                                                     <  2:3518439/99‑1 (MQ=255)
    cgcccgggCCGCGGTCCCCCTCCCCTTGTAATCCCCCC.GGGCCCCGCCACCGTCACCGCCC.GCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGAt                                                                                                  <  2:6617320/95‑1 (MQ=255)
       ccgggCCGCGTCCGCCCTCCCCTTCTGATCCCCTG.GGGCGCCGCCCCCGCCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                               <  2:6528228/98‑1 (MQ=255)
       cccGGCCGCGGTCCCCCTCCCCTTCTGATCCCCTC.GGGCGCCGCCACCGTCCCCCCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                               <  2:672595/101‑1 (MQ=255)
        ccGGCCGCGGTCGCCCTCCCCTTCTGATGCCCTC.GGGCGCCGTCCCCGTCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                              <  2:3070757/101‑1 (MQ=255)
         cGGCCGCGCCCGCCCTCCCCTTCTGATGCCCTC.GGGCGCCGTCCCCGTCCCCGCCC.GCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAg                                                                                             <  2:4617864/101‑1 (MQ=255)
            ccgggcccccccTCACCTTCTGATGCCCTC.GGGCGCCGTCCCCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                          <  1:4011944/92‑1 (MQ=255)
              gcgGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAggggac                                                                                        >  1:9085300/1‑99 (MQ=255)
              gcgGTCCCCCTCCCCTTTTGCTCCCCGC.GGGCGCCGTCACCGTCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGcc                                                                                        <  2:6361015/101‑1 (MQ=255)
               cggccccccTCCCCTTCGGATCCCCTG.GGGCGCCGTCCCCGTCCCCGCCC.GCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                       <  2:5376020/95‑1 (MQ=255)
                ggccccccTCCCCTCCGGAGGCCCTC.GGCCGCCGCCCCCGTCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      <  2:6218624/96‑1 (MQ=255)
                ggTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      >  2:931687/1‑101 (MQ=255)
                ggTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      >  1:7139608/1‑101 (MQ=255)
                ggTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      >  2:8421384/1‑101 (MQ=255)
                ggTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      >  2:806917/1‑101 (MQ=255)
                ggTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCCGGCGGCAAGCCCCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCTTc                                                                                      >  2:795528/1‑101 (MQ=255)
                 gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                     >  2:3650093/1‑101 (MQ=255)
                 gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                     >  1:1717708/1‑101 (MQ=255)
                 gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                     >  1:8378097/1‑101 (MQ=255)
                 gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                     >  1:7952221/1‑101 (MQ=255)
                  ccccccTCCCCTTCTGATCCCCCC.GGGCGCCGCCCCCGCCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                    <  1:6028080/98‑1 (MQ=255)
                   ccccccccccTTCTGATCCCCCC.GGGCGCCGTCCCCGTCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                   <  1:6746193/93‑1 (MQ=255)
                   cGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                   >  1:5646980/1‑101 (MQ=255)
                    ccccTCACCTTCTGACGCCCTC.GGGCCCCGTCCCCGCCCCCCCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                  <  1:4405005/100‑1 (MQ=255)
                     cccTCCCCTTCTGAGGCCCTC.GGGCGCCGTCCCCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                 <  1:8968806/101‑1 (MQ=255)
                      ccTCCCCTTCTGATGCCCTC.GGGCGCCGCCCCCGCCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                <  2:2374658/101‑1 (MQ=255)
                      ccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                >  2:5914786/1‑101 (MQ=255)
                      ccTCACCTTCTGAGCCCCTC.GGGCGCCGCCCCCGCCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                <  2:220316/101‑1 (MQ=255)
                       cTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                               >  1:867590/1‑101 (MQ=255)
                        ccACCTTCTGAGCCCCGC.GGGCGCCGTCACCCTCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCt                                                                              <  2:6983879/100‑1 (MQ=255)
                         ccccTTCTGATGCCCGC.GGGCGCCGTCCCCGTCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                             <  2:3183630/99‑1 (MQ=255)
                         cACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                             >  1:3329506/1‑101 (MQ=255)
                          aCCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                            >  2:1973352/1‑101 (MQ=255)
                          aCCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                            >  1:4519130/1‑101 (MQ=255)
                           ccTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgt                                                                           >  1:8147974/1‑101 (MQ=255)
                              tCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                        >  2:8368851/1‑101 (MQ=255)
                               cgggtccccCC.GGGCGCCGTCCCCGTCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGAt                                                                       <  1:931700/95‑1 (MQ=255)
                                  tgccccTCGGGGCGCGGCCCCCGCCCCCCCGC.GCGGGGGGCCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                     <  1:3216713/98‑1 (MQ=255)
                                     cccGC.GGGCGCCCCCCCCGCCCCCCCCC.GCGGGGGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATc                                                                 <  1:1096588/101‑1 (MQ=255)
                                      ccgc.GGGCGCCGTCCCCGCCCCCCCCC.GCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCt                                                                <  1:3695827/98‑1 (MQ=255)
                                      cccggggccgcCGCCACCGCCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATc                                                                 <  2:2568510/93‑1 (MQ=255)
                                      ccTG.GGGCGCCGCCACCCTCCCCCCCC.GCCGGGCGCAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCt                                                                <  2:1751478/101‑1 (MQ=255)
                                          ggggCCCCGCCCCCGCCCCCCCCC.GCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                             <  1:3840079/100‑1 (MQ=255)
                                                ccgtcaccgtcaCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcgggg                                                       >  1:5041990/1‑101 (MQ=255)
                                                  gtcatcgtcaccGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                     >  1:6221665/1‑101 (MQ=255)
                                                   tcaccgtcaccgCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCg                                                    >  1:6371021/1‑101 (MQ=255)
                                                   tcaccgtcaccgCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCg                                                    >  2:1043615/1‑101 (MQ=255)
                                                     ccccgtcccgcccggCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                   <  2:5646773/94‑1 (MQ=255)
                                                          cccccccc.gcgGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcaccac                                             <  1:6801418/96‑1 (MQ=255)
                                                           ccctccc.ggcGGTGGGAAAGCCGCGAAAGGTCAGTATCATTAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                            <  2:4975451/97‑1 (MQ=255)
                                                           cccgccc.gcGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                            <  1:8068840/100‑1 (MQ=255)
                                                           accgCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                            >  2:7698523/1‑101 (MQ=255)
                                                                   gccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcgg                                     >  1:3478947/1‑101 (MQ=255)
                                                                     cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                   >  1:2946962/1‑101 (MQ=255)
                                                                          ggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                              >  1:2468307/1‑101 (MQ=255)
                                                                          gacAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                              <  2:8830005/99‑1 (MQ=255)
                                                                            cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                            >  2:4008369/1‑101 (MQ=255)
                                                                            cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                            >  2:6893493/1‑101 (MQ=255)
                                                                            aaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                            <  1:4204462/100‑1 (MQ=255)
                                                                               gCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCg                         <  1:5483543/101‑1 (MQ=255)
                                                                                ccGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCgg                        >  1:5704567/1‑101 (MQ=255)
                                                                                  gcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTAGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggga                      >  2:4430280/1‑100 (MQ=255)
                                                                                    gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                    >  1:8481187/1‑101 (MQ=255)
                                                                                    gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                    >  2:560040/1‑101 (MQ=255)
                                                                                       aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                 >  2:427969/1‑101 (MQ=255)
                                                                                          agaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtg              <  2:9069203/93‑1 (MQ=255)
                                                                                           cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt             <  2:7319206/101‑1 (MQ=255)
                                                                                            aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTTGATGCCATCTGGGGCGGGGCCGTCTCCACGATGGCGGCGGCGCTCGCCGTGCGGCTGAtgtg            <  1:6571163/101‑1 (MQ=255)
                                                                                             ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg           >  1:1158685/1‑101 (MQ=255)
                                                                                             aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg           <  2:7475742/96‑1 (MQ=255)
                                                                                             aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCCAATGTgg           >  1:364252/6‑101 (MQ=255)
                                                                                              gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc          <  1:7027937/101‑1 (MQ=255)
                                                                                              gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc          >  1:2363283/1‑101 (MQ=255)
                                                                                              gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc          >  2:1496761/1‑101 (MQ=255)
                                                                                              gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc          <  1:3941089/101‑1 (MQ=255)
                                                                                              aaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGc          <  1:182817/97‑1 (MQ=255)
                                                                                               aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGGTGTGGCa         >  1:2731983/1‑101 (MQ=255)
                                                                                               aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa         <  1:7769340/101‑1 (MQ=255)
                                                                                               aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa         >  1:6939138/1‑101 (MQ=255)
                                                                                                aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGGAt        >  2:2887484/3‑101 (MQ=255)
                                                                                                aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt        <  2:7361019/99‑1 (MQ=255)
                                                                                                 cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       <  1:5891151/101‑1 (MQ=255)
                                                                                                 cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       <  2:7877046/101‑1 (MQ=255)
                                                                                                 cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       >  2:2084139/1‑101 (MQ=255)
                                                                                                 cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       >  1:5248911/1‑101 (MQ=255)
                                                                                                 cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCCGCTGTGGCAtt       >  2:1386037/1‑101 (MQ=255)
                                                                                                 aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt       >  1:2174053/2‑101 (MQ=255)
                                                                                                  aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      <  2:6842036/101‑1 (MQ=255)
                                                                                                  aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc      <  1:7286619/101‑1 (MQ=255)
                                                                                                   tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg     <  2:7081825/101‑1 (MQ=255)
                                                                                                   tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg     >  1:1294228/1‑101 (MQ=255)
                                                                                                   tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg     <  1:4638898/101‑1 (MQ=255)
                                                                                                   tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg     <  2:1192705/101‑1 (MQ=255)
                                                                                                   tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg     >  2:6068774/1‑101 (MQ=255)
                                                                                                   tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg     <  1:1522166/101‑1 (MQ=255)
                                                                                                   tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg     <  1:7592762/101‑1 (MQ=255)
                                                                                                      gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc  >  2:4792511/1‑101 (MQ=255)
                                                                                                      gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc  >  2:450486/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACC  >  NC_014034/2155340‑2155540

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG