Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,445 G→A 19.4% A4T (GCA→ACC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4450G→A19.4% 193.0 ‑1.4 ‑0.1406A4T (GCA→ACA) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (34/45):  ref base (166/161):  total (200/206)
Fisher's exact test strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.24e-01

Only 100 of 417 total aligned reads displayed.

ACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGT  >  NC_014034/2155346‑2155545
                                                                                                    |                                                                                                    
ccccGGCCGCGGCCCCCCTCCCCTTCGGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGc                                                                                                     <  1:1145002/100‑1 (MQ=255)
 ccgggCCGCGTCCGCCCTCCCCTTCTGATCCCCTGGGGCGCCGCCCCCGCCCCCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                                    <  2:6528228/98‑1 (MQ=255)
 cccGGCCTGGGTCGCCCTCCCTTTCGGAGGCCCTGGGGCGCCGTCACCGTCCCCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                                    <  2:5619726/101‑1 (MQ=255)
 cccGCCCGCGGTCCCCCTCACCTTCTGATGCCCGCGGGCGCCGCCACCCTCCCCGCCCGCG.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                                    <  1:3708707/101‑1 (MQ=255)
  ccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                                   >  2:4733600/1‑101 (MQ=255)
  ccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                                   >  2:7015170/1‑101 (MQ=255)
    ggCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.CGGCGGCAAGCCGCGCAAGGGCAGGATCATGAGCGATGCAAgg                                                                                                 >  1:1443928/1‑101 (MQ=255)
     gcggcgGTCGCCCTCCCCTTCTGATGCCCTGGGGCGCCGTCCCCGTCACCGCCCGCC.GGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAggg                                                                                                <  2:1134756/98‑1 (MQ=255)
        gcgGTCCCCCTCCCCTTTTGCTCCCCGCGGGCGCCGTCACCGTCCCCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGcc                                                                                             <  2:6361015/101‑1 (MQ=255)
          ggccccccTCCCCTCCGGAGGCCCTCGGCCGCCGCCCCCGTCCCCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                           <  2:6218624/96‑1 (MQ=255)
          ggTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                           >  2:8421384/1‑101 (MQ=255)
           gTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                          >  2:3650093/1‑101 (MQ=255)
           gTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                          >  1:7952221/1‑101 (MQ=255)
            tccccccccccTTCTGATGCCCGCGGGCGCCGCCCCCGTCCCCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                         <  1:8365938/92‑1 (MQ=255)
            gcccccgccccTTCTGATCCCCGCGGGCCCCGTCCCCGTCCCCGCCCGCG.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                         <  2:3029325/92‑1 (MQ=255)
             cGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGGTCATGAGCGATGCAAGGGGCCTCGTa                                                                                        >  2:9154237/1‑101 (MQ=255)
             cGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                        >  2:5688781/1‑101 (MQ=255)
                ccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                     >  2:3398244/1‑101 (MQ=255)
                ccTCACCTTCTGAGCCCCTCGGGCGCCGCCCCCGCCCCCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                     <  2:220316/101‑1 (MQ=255)
                 cTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                                    >  1:8455148/1‑101 (MQ=255)
                    aCCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                                 >  2:1973352/1‑101 (MQ=255)
                    aCCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                                 >  1:4519130/1‑101 (MQ=255)
                       ttCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtcg                                                                              >  1:5656346/1‑101 (MQ=255)
                        tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                             >  2:8368851/1‑101 (MQ=255)
                        tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                             >  2:8068500/1‑101 (MQ=255)
                            aTGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                         >  1:874114/1‑101 (MQ=255)
                               cccggggccgcCGCCACCGCCCCCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATc                                                                      <  2:2568510/93‑1 (MQ=255)
                                ccTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCt                                                                     >  1:5719663/1‑101 (MQ=255)
                                    gggCGCGGTCCCCGCCCCCGCCCGCG.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                                 <  2:6704871/101‑1 (MQ=255)
                                     ggCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggc                                                                >  1:74145/1‑101 (MQ=255)
                                           gtcaccgtcaccGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                          >  1:953702/1‑101 (MQ=255)
                                            tcaccgtcaccgCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCg                                                         >  1:4764868/1‑101 (MQ=255)
                                             caccgtcaccgCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                        >  2:1694077/1‑101 (MQ=255)
                                                 gtcaccgCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                    >  1:1530449/1‑101 (MQ=255)
                                                   ccacggccgccgGGGGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacca                                                   <  1:7015455/95‑1 (MQ=255)
                                                    ccctcccggc.GGTGGGAAAGCCGCGAAAGGTCAGTATCATTAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                 <  2:4975451/97‑1 (MQ=255)
                                                    cccgcccgcG.GGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                 <  2:3145222/100‑1 (MQ=255)
                                                    cccgcccgcG.GGGCGCAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                 <  2:8766518/100‑1 (MQ=255)
                                                    accgCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                 >  2:7698523/1‑101 (MQ=255)
                                                               gggCGCAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                       <  1:8512940/101‑1 (MQ=255)
                                                                  cggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGgcgc                                    >  1:2192454/1‑101 (MQ=255)
                                                                   ggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                                   >  1:2468307/1‑101 (MQ=255)
                                                                   ggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                                   >  1:1773682/1‑101 (MQ=255)
                                                                   ggaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                                   <  1:4418002/98‑1 (MQ=255)
                                                                     cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                 >  2:6893493/1‑101 (MQ=255)
                                                                     aaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                 <  1:4204462/100‑1 (MQ=255)
                                                                      aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                                >  1:3315240/1‑101 (MQ=255)
                                                                      aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCCCGc                                >  1:6041266/1‑101 (MQ=255)
                                                                           gcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggc                           <  1:3861241/101‑1 (MQ=255)
                                                                            cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                          >  1:7261645/1‑101 (MQ=255)
                                                                              aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGGGGCGCTCGCCGggcggg                        >  2:4160428/1‑100 (MQ=255)
                                                                              aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                        >  1:7135665/1‑101 (MQ=255)
                                                                              aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCCGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                        >  1:1884301/1‑101 (MQ=255)
                                                                               aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                       >  1:6872828/1‑101 (MQ=255)
                                                                                aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                      <  1:3650201/101‑1 (MQ=255)
                                                                                aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                      <  2:7294309/101‑1 (MQ=255)
                                                                                    gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                  <  1:811850/94‑1 (MQ=255)
                                                                                    cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt                  <  1:1274429/101‑1 (MQ=255)
                                                                                    cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt                  <  1:6746955/101‑1 (MQ=255)
                                                                                     aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg                 <  2:5401575/95‑1 (MQ=255)
                                                                                     aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                 >  2:4408974/1‑101 (MQ=255)
                                                                                      ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGATCGCCGGGCGGCTGATGTgg                <  1:9140559/101‑1 (MQ=255)
                                                                                      aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg                <  2:7475742/96‑1 (MQ=255)
                                                                                       gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc               >  2:1496761/1‑101 (MQ=255)
                                                                                       gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc               <  1:7866556/101‑1 (MQ=255)
                                                                                       ctaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGc               <  2:5998987/97‑1 (MQ=255)
                                                                                       aaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGc               >  1:4153267/5‑101 (MQ=255)
                                                                                        aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa              >  1:3565053/4‑101 (MQ=255)
                                                                                         aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGGAt             >  2:2887484/3‑101 (MQ=255)
                                                                                         aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCag             >  1:5999222/3‑100 (MQ=255)
                                                                                         aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt             <  2:7361019/99‑1 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGTGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            >  1:6258817/1‑101 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            <  2:7877046/101‑1 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            >  1:3371623/1‑101 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            >  2:2084139/1‑101 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCCGCTGTGGCAtt            >  2:1386037/1‑101 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCCGGCGGCTGATGGGGCAtt            >  1:5155165/1‑101 (MQ=255)
                                                                                          aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt            <  1:3945148/100‑1 (MQ=255)
                                                                                           aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGTCATTc           <  2:4523947/101‑1 (MQ=255)
                                                                                           aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc           >  1:5281019/1‑101 (MQ=255)
                                                                                           aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc           <  2:6842036/101‑1 (MQ=255)
                                                                                           aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc           >  1:6661443/1‑101 (MQ=255)
                                                                                           aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc           >  2:8902977/1‑101 (MQ=255)
                                                                                           aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc           <  1:2591844/101‑1 (MQ=255)
                                                                                           aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc           <  1:6461434/101‑1 (MQ=255)
                                                                                            tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg          <  2:7081825/101‑1 (MQ=255)
                                                                                            tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg          >  2:6068774/1‑101 (MQ=255)
                                                                                            tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg          <  1:3138003/101‑1 (MQ=255)
                                                                                            tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg          <  1:7592762/101‑1 (MQ=255)
                                                                                            tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg          <  2:2444946/101‑1 (MQ=255)
                                                                                             gaccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa         >  1:2799912/4‑101 (MQ=255)
                                                                                               gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc       <  2:4483429/101‑1 (MQ=255)
                                                                                               gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc       <  1:5503858/101‑1 (MQ=255)
                                                                                               gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc       <  1:8999797/101‑1 (MQ=255)
                                                                                                cGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGGTGTGGCATTCGACCg      >  1:2273085/1‑101 (MQ=255)
                                                                                                cGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCg      <  2:7951881/101‑1 (MQ=255)
                                                                                                cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg      <  2:536530/101‑1 (MQ=255)
                                                                                                 gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa     <  1:1043633/101‑1 (MQ=255)
                                                                                                   tGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAgg   <  2:5855137/101‑1 (MQ=255)
                                                                                                    gCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGt  >  2:3964231/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
ACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCC.GGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGT  >  NC_014034/2155346‑2155545

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG