Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,463 C→G 34.3% L10V (CTC→GTC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4630C→G34.3% 0.0 ‑1.4 ‑0.6495L10V (CTC→GTC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (89/81):  ref base (188/137):  total (277/218)
Fisher's exact test strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.76e-01

Only 100 of 508 total aligned reads displayed.

CTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCG  >  NC_014034/2155363‑2155563
                                                                                                     |                                                                                                    
cTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                                                      >  1:8455148/1‑101 (MQ=255)
   aCCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                                                   >  1:4519130/1‑101 (MQ=255)
         tGATGCCCTCGGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                                             >  2:6365556/1‑101 (MQ=255)
           aTGCCCTCGGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                                           >  1:874114/1‑101 (MQ=255)
             gCCCTCGGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                                         >  2:6050379/1‑101 (MQ=255)
             gCCCGCGGGCGCCGCCGCCGCCCCCCCCC.GCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                                         <  2:5824187/101‑1 (MQ=255)
              cccggggccgcCGCCACCGCCCCCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATc                                                                                        <  2:2568510/93‑1 (MQ=255)
                   gggCGCGGTCCCCGCCCCCGCCC.GCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                                                   <  2:6704871/101‑1 (MQ=255)
                   gggCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                                                   >  1:3714228/1‑101 (MQ=255)
                    ggCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggc                                                                                  >  1:74145/1‑101 (MQ=255)
                          gtcaccgtcaccGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                                            >  1:953702/1‑101 (MQ=255)
                          gtcaccgtcaccGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                                            >  1:7766925/1‑101 (MQ=255)
                           tcaccgtcaccgCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCg                                                                           >  1:4764868/1‑101 (MQ=255)
                            caccgtcaccgCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                                          >  1:3873418/1‑101 (MQ=255)
                             ccctcccccgccc.ggCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                                         <  2:722515/94‑1 (MQ=255)
                             ccccgtcccgcccggCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                                          <  2:5646773/94‑1 (MQ=255)
                                gtcaccgCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                                      >  1:6307581/1‑101 (MQ=255)
                                ccgcccgccc.gcCGGGGGCAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                                      <  2:462494/97‑1 (MQ=255)
                                   cccgccg.gcCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                                   <  2:1973813/100‑1 (MQ=255)
                                           gccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcgg                                                            >  1:3478947/1‑101 (MQ=255)
                                            ccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                                           >  2:1775563/1‑101 (MQ=255)
                                             cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                                          >  1:6763598/1‑101 (MQ=255)
                                                  ggaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                                                     <  1:4418002/98‑1 (MQ=255)
                                                   gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                                    >  2:4974060/1‑101 (MQ=255)
                                                    cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                                   >  1:5585180/1‑101 (MQ=255)
                                                    aaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                                   <  1:4204462/100‑1 (MQ=255)
                                                      aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                                                 <  1:7659955/101‑1 (MQ=255)
                                                        ccGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCgg                                               >  1:5704567/1‑101 (MQ=255)
                                                            gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                                           >  2:278555/1‑101 (MQ=255)
                                                              aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggcg                                         >  1:327117/1‑100 (MQ=255)
                                                              aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                                         >  1:1617972/1‑101 (MQ=255)
                                                                 gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt                                      <  2:2108824/92‑1 (MQ=255)
                                                                  gCAGGATGATGAGCGATGCAAGGGTCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                                     <  2:5181281/101‑1 (MQ=255)
                                                                  agaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtg                                     <  2:9069203/93‑1 (MQ=255)
                                                                   cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt                                    <  1:1274429/101‑1 (MQ=255)
                                                                    aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATACCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtggg                                   >  2:4429984/7‑99 (MQ=255)
                                                                    aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTTGATGCCATCTGGGGCGGGGCCGTCTCCACGATGGCGGCGGCGCTCGCCGTGCGGCTGAtgtg                                   <  1:6571163/101‑1 (MQ=255)
                                                                     ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGATCGCCGGGCGGCTGATGTgg                                  <  1:9140559/101‑1 (MQ=255)
                                                                     aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg                                  <  2:7475742/96‑1 (MQ=255)
                                                                      gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc                                 <  1:7027937/101‑1 (MQ=255)
                                                                      gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc                                 <  1:7866556/101‑1 (MQ=255)
                                                                      gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc                                 >  2:1496761/1‑101 (MQ=255)
                                                                      ctaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGc                                 <  2:5998987/97‑1 (MQ=255)
                                                                      aaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGc                                 <  1:182817/97‑1 (MQ=255)
                                                                       aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa                                >  2:6854374/4‑101 (MQ=255)
                                                                       aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa                                >  1:6939138/1‑101 (MQ=255)
                                                                        aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt                               >  2:8336580/3‑101 (MQ=255)
                                                                         cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt                              >  1:3371623/1‑101 (MQ=255)
                                                                         cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt                              <  2:7877046/101‑1 (MQ=255)
                                                                         cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCCGCTGTGGCAtt                              >  2:1386037/1‑101 (MQ=255)
                                                                          aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                             <  2:3077581/101‑1 (MQ=255)
                                                                          aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                             <  2:7343218/101‑1 (MQ=255)
                                                                          aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                             <  1:2388478/101‑1 (MQ=255)
                                                                          aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                             <  2:6957006/101‑1 (MQ=255)
                                                                          aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                             >  1:5281019/1‑101 (MQ=255)
                                                                           tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg                            <  2:1192705/101‑1 (MQ=255)
                                                                            gaccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa                           >  1:2799912/4‑101 (MQ=255)
                                                                             accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc                          >  2:6169219/3‑101 (MQ=255)
                                                                             accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc                          <  2:3421854/99‑1 (MQ=255)
                                                                             accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATCCGAc                          >  1:8557781/3‑101 (MQ=255)
                                                                              gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc                         >  2:450486/1‑101 (MQ=255)
                                                                              gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc                         >  2:4792511/1‑101 (MQ=255)
                                                                                gataccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGa                       >  1:7145224/10‑101 (MQ=255)
                                                                                gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGTCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa                       >  1:6010669/1‑101 (MQ=255)
                                                                                gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa                       <  1:1043633/101‑1 (MQ=255)
                                                                                 ataccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAg                      >  2:7548655/9‑101 (MQ=255)
                                                                                 aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg                      >  2:8483583/1‑101 (MQ=255)
                                                                                   accagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGt                    <  1:2575455/95‑1 (MQ=255)
                                                                                     cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc                  <  2:7948622/97‑1 (MQ=255)
                                                                                     aaGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGc                  <  1:6687515/101‑1 (MQ=255)
                                                                                     aaGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGc                  >  1:2196820/1‑101 (MQ=255)
                                                                                      agaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCg                 >  2:3991293/4‑101 (MQ=255)
                                                                                      agaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCg                 >  2:4340399/4‑101 (MQ=255)
                                                                                      aGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCg                 >  2:8067049/1‑101 (MQ=255)
                                                                                      aGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGGGCg                 >  2:6570945/1‑101 (MQ=255)
                                                                                        aggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGAAGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCggg               >  2:7741939/2‑101 (MQ=255)
                                                                                         ggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgc              <  2:2427595/101‑1 (MQ=255)
                                                                                          gCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcg             <  1:3036070/101‑1 (MQ=255)
                                                                                           ccTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgc            <  2:704775/101‑1 (MQ=255)
                                                                                            cTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCTGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcg           >  1:8862186/1‑101 (MQ=255)
                                                                                            cTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcg           <  1:5143561/101‑1 (MQ=255)
                                                                                              cGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcggc         >  1:1444727/1‑99 (MQ=255)
                                                                                              cGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcggc         <  2:3879999/101‑3 (MQ=255)
                                                                                              cGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCa         >  1:2326717/1‑101 (MQ=255)
                                                                                               gtcgagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcggggcg        >  1:7332377/4‑96 (MQ=255)
                                                                                               gtcgagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcggcg        <  1:5386296/98‑4 (MQ=255)
                                                                                               gtcgagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcggcg        >  1:3645612/4‑98 (MQ=255)
                                                                                               gTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCaa        >  1:6185794/1‑101 (MQ=255)
                                                                                                tcgagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcggcgg       >  1:4116596/3‑97 (MQ=255)
                                                                                                tAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCTGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAg       <  2:3266251/101‑1 (MQ=255)
                                                                                                 agagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgc      >  2:5430839/1‑101 (MQ=255)
                                                                                                 agagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgc      <  1:7529449/101‑1 (MQ=255)
                                                                                                 agagCTCGTCGATGACATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgc      <  1:7971879/101‑1 (MQ=255)
                                                                                                  gagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgcg     >  1:1975035/1‑101 (MQ=255)
                                                                                                  gagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgcg     >  1:814252/1‑101 (MQ=255)
                                                                                                  gagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgcg     >  2:1621074/1‑101 (MQ=255)
                                                                                                  gagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgcg     >  1:1126294/1‑101 (MQ=255)
                                                                                                  gagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgcg     <  2:4169086/101‑1 (MQ=255)
                                                                                                    gCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGcgcc   >  2:8841442/1‑101 (MQ=255)
                                                                                                     cTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGcgccg  >  1:5884198/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
CTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCC.GCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCG  >  NC_014034/2155363‑2155563

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG