Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,176,472 G→C 100% A302G (GCG→GGG)  rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,176,4720G→C100.0% 1803.1 482A302G (GCG→GGG) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (246/235):  ref base (0/0):  total (247/235)

Only 100 of 491 total aligned reads displayed.

CCCAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATG.CCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCGCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTGGC  >  NC_014034/2176375‑2176569
                                                                                                  |                                                                                                 
 ccAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGt                                                                                                >  1:8756951/1‑101 (MQ=255)
      aTTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGAAGAAATCGAGCCCGTCGGaa                                                                                           >  2:6924504/1‑101 (MQ=255)
       ttGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAAt                                                                                          >  1:625248/1‑101 (MQ=255)
       ttGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAAt                                                                                          <  1:5187773/101‑1 (MQ=255)
        tGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAAtt                                                                                         >  2:834296/1‑101 (MQ=255)
        tGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAAtt                                                                                         >  1:8483414/1‑101 (MQ=255)
         gTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTc                                                                                        >  2:2191586/1‑101 (MQ=255)
         gTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTc                                                                                        >  1:7929398/1‑101 (MQ=255)
         gTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTc                                                                                        >  1:4928560/1‑101 (MQ=255)
         gTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGAGTTGACGAAATCGAGCCCGTCGGAATTc                                                                                        >  1:1026169/1‑101 (MQ=255)
          tttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCa                                                                                       <  1:6531436/101‑1 (MQ=255)
           ttCAGCGCCTCTGCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCAc                                                                                      <  2:1527900/101‑1 (MQ=255)
            tCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCAcc                                                                                     >  2:3876607/1‑101 (MQ=255)
              aGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCgg                                                                                   >  1:5263187/1‑101 (MQ=255)
                 gcCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCtt                                                                                >  2:5067632/1‑101 (MQ=255)
                     ctTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTg                                                                            >  2:8273531/1‑101 (MQ=255)
                     ctTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTg                                                                            >  1:7112329/1‑101 (MQ=255)
                       tCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGcc                                                                          >  2:5475444/1‑101 (MQ=255)
                       tCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGcc                                                                          >  1:380466/1‑101 (MQ=255)
                         cGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGt                                                                        <  1:7428518/101‑1 (MQ=255)
                         cGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGt                                                                        >  2:2759626/1‑101 (MQ=255)
                         cGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGt                                                                        >  1:1668161/1‑101 (MQ=255)
                          gAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       >  1:7271577/1‑101 (MQ=255)
                          gAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       <  1:3179651/101‑1 (MQ=255)
                          gAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       >  2:9200066/1‑101 (MQ=255)
                           aGGTGAGCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTcc                                                                      <  1:2391709/101‑1 (MQ=255)
                             gTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGATCCCGTCGGAATTCACCGGCTTCGTGCCGTCCtt                                                                    >  1:5020550/1‑101 (MQ=255)
                             gTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCtt                                                                    >  2:7980439/1‑101 (MQ=255)
                               gATCGAGGGGATGCCCCCGATAGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTgg                                                                  <  1:2046150/101‑1 (MQ=255)
                                aTCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGc                                                                 >  2:4481292/1‑101 (MQ=255)
                                   gAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATa                                                              <  1:4023632/101‑1 (MQ=255)
                                   gAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATa                                                              <  1:348565/101‑1 (MQ=255)
                                    aGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAg                                                             <  2:4074325/101‑1 (MQ=255)
                                    aGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAg                                                             >  1:5111796/1‑101 (MQ=255)
                                    aGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAg                                                             >  2:5314358/1‑101 (MQ=255)
                                     ggggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAgg                                                            >  1:4301568/1‑101 (MQ=255)
                                     ggggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAgg                                                            <  2:8491188/101‑1 (MQ=255)
                                      gggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAggg                                                           >  2:5625149/1‑100 (MQ=255)
                                      gggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAggc                                                           <  2:9058569/101‑1 (MQ=255)
                                         atgcccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCGTTGGCATAggcggc                                                        >  2:3080469/5‑101 (MQ=255)
                                          tccccgcGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGgcggcg                                                       <  2:5966952/95‑1 (MQ=255)
                                             ccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATc                                                    <  1:5395347/101‑1 (MQ=255)
                                             ccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATc                                                    <  2:7701401/101‑1 (MQ=255)
                                              cccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCg                                                   >  1:1350814/1‑101 (MQ=255)
                                                cGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGGTCGcc                                                 >  2:4835722/1‑101 (MQ=255)
                                                  aTCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTc                                               <  2:7125587/101‑1 (MQ=255)
                                                  aTCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTc                                               <  1:782974/101‑1 (MQ=255)
                                                  aTCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTc                                               <  1:2702994/101‑1 (MQ=255)
                                                   tCGGGCTGTCGGTGACCCGCGTCACCCCGGTGTTGACGAAATTGAGTCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCg                                              <  2:1653567/101‑1 (MQ=255)
                                                    cGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGa                                             >  2:4189042/1‑101 (MQ=255)
                                                     gggCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCgtccaggt                                            >  2:3228864/1‑94 (MQ=255)
                                                      ggCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATg                                           <  1:7564324/101‑1 (MQ=255)
                                                       gCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGc                                          <  1:1853720/101‑1 (MQ=255)
                                                        cTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGcc                                         <  1:6674033/101‑1 (MQ=255)
                                                         tGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGccc                                        >  1:6589733/1‑101 (MQ=255)
                                                          gTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCt                                       <  2:7506851/101‑1 (MQ=255)
                                                          gTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCt                                       <  1:4481427/101‑1 (MQ=255)
                                                           tCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCtt                                      <  2:4260010/101‑1 (MQ=255)
                                                           tCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCtt                                      <  1:5882251/101‑1 (MQ=255)
                                                            gggTGACCCGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTg                                     <  1:5598620/100‑1 (MQ=255)
                                                            cGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTg                                     >  2:293217/1‑101 (MQ=255)
                                                             ggTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTgg                                    <  1:69619/101‑1 (MQ=255)
                                                             ggTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTgg                                    <  1:6857431/101‑1 (MQ=255)
                                                             ggTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCCCCTCGATGCCCTTgg                                    <  2:358222/101‑1 (MQ=255)
                                                               tGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTggcg                                  <  1:5475629/101‑3 (MQ=255)
                                                               tGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAg                                  >  1:2599684/1‑101 (MQ=255)
                                                                 aCCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGc                                <  2:6663911/101‑1 (MQ=255)
                                                                   cAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGccc                              >  2:1082750/1‑100 (MQ=255)
                                                                    aGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCAt                             >  1:6271877/1‑101 (MQ=255)
                                                                      cGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTTGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCa                           >  1:5715005/1‑101 (MQ=255)
                                                                      cGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCCTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCa                           >  2:5225029/1‑101 (MQ=255)
                                                                       gTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAg                          >  1:3300778/1‑101 (MQ=255)
                                                                       gTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAg                          <  2:8135510/101‑1 (MQ=255)
                                                                       gTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAg                          >  2:6026501/1‑101 (MQ=255)
                                                                       gTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAg                          >  1:4598918/1‑101 (MQ=255)
                                                                       gTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAg                          >  2:3492720/1‑101 (MQ=255)
                                                                         cACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCTTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGaa                        <  2:625238/101‑1 (MQ=255)
                                                                         cACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGaa                        >  2:2546531/1‑101 (MQ=255)
                                                                             ccGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCgg                    >  2:9171916/1‑101 (MQ=255)
                                                                               ggTTTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGa                  <  2:3440035/101‑1 (MQ=255)
                                                                                gtgtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGaa                 >  1:4192002/1‑101 (MQ=255)
                                                                                 tgtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAt                <  2:6142097/101‑1 (MQ=255)
                                                                                  gtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAtt               <  1:4732979/101‑1 (MQ=255)
                                                                                  gtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAtt               >  1:336294/1‑101 (MQ=255)
                                                                                  gtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAtt               >  1:3742017/1‑101 (MQ=255)
                                                                                    tGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGc             >  1:3589658/1‑101 (MQ=255)
                                                                                    tGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGc             <  1:3908040/101‑1 (MQ=255)
                                                                                    ggACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGc             <  1:4137870/100‑1 (MQ=255)
                                                                                     gACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCa            <  2:8652182/101‑1 (MQ=255)
                                                                                      aCGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCAt           >  1:8086449/1‑101 (MQ=255)
                                                                                      aCGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCAt           >  2:1914210/1‑101 (MQ=255)
                                                                                        gAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCg         >  2:7036472/1‑101 (MQ=255)
                                                                                        gAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCg         <  2:3686720/101‑1 (MQ=255)
                                                                                        gAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCg         >  2:4389972/1‑101 (MQ=255)
                                                                                        caaaTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCg         <  1:2919391/100‑1 (MQ=255)
                                                                                         aaaTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGa        <  1:9147821/101‑1 (MQ=255)
                                                                                          aaTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAg       >  1:2157436/1‑101 (MQ=255)
                                                                                          aaTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAg       >  2:1334836/1‑101 (MQ=255)
                                                                                            tCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGt     <  2:7271273/101‑1 (MQ=255)
                                                                                              gAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTgg   >  1:1132977/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CCCAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATG.CCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCGCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTGGC  >  NC_014034/2176375‑2176569

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG