Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,213 G→A 100% P55L (CCC→CTC)  rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2130G→A100.0% 1740.5 445P55L (CCC→CTC) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (226/219):  ref base (0/0):  total (226/219)

Only 100 of 453 total aligned reads displayed.

GCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGT  >  NC_014034/2177115‑2177305
                                                                                                  |                                                                                                 
gCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGc                                                                                                 <  2:1753897/101‑1 (MQ=255)
gCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGc                                                                                                 <  1:9149146/101‑1 (MQ=255)
gCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGc                                                                                                 >  1:1673114/1‑101 (MQ=255)
 cGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCa                                                                                                >  1:6746295/1‑101 (MQ=255)
 cGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCa                                                                                                <  2:1468463/101‑1 (MQ=255)
 cGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCa                                                                                                >  2:3036831/1‑101 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               <  1:3131199/101‑1 (MQ=255)
   tGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAcc                                                                                              <  1:3438294/101‑1 (MQ=255)
    gATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGTCCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCa                                                                                             <  2:1085802/101‑1 (MQ=255)
     aTCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAg                                                                                            <  1:1249345/101‑1 (MQ=255)
      tCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGc                                                                                           >  2:2776324/1‑101 (MQ=255)
       cAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGcc                                                                                          >  2:2815946/1‑101 (MQ=255)
         aaaTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGcccc                                                                                        >  1:1072120/1‑101 (MQ=255)
          aaTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCa                                                                                       >  1:3994181/1‑101 (MQ=255)
           aTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAt                                                                                      <  2:129816/101‑1 (MQ=255)
           aTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAt                                                                                      <  2:7606289/101‑1 (MQ=255)
           aTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAt                                                                                      <  2:666700/101‑1 (MQ=255)
           aTCCCCGTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAt                                                                                      >  1:5727071/1‑101 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    <  2:435425/101‑1 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    >  2:7503400/1‑101 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    <  2:6557446/101‑1 (MQ=255)
               ccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCg                                                                                  >  1:3736058/1‑101 (MQ=255)
                cTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCgg                                                                                 <  1:3080568/101‑1 (MQ=255)
                cTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCgg                                                                                 <  1:5569114/101‑1 (MQ=255)
                  ttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTc                                                                               <  1:8235869/101‑1 (MQ=255)
                  ttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTc                                                                               >  2:192431/1‑101 (MQ=255)
                   tGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCg                                                                              >  2:7122722/1‑101 (MQ=255)
                   tGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCg                                                                              >  2:5849460/1‑101 (MQ=255)
                     ccccGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGcc                                                                            <  1:9010375/101‑1 (MQ=255)
                      cccgcccgCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCt                                                                           <  2:1202396/101‑1 (MQ=255)
                       ccgcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCtt                                                                          >  2:6080854/1‑101 (MQ=255)
                        cgcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATATGTCGAGAGCACCAGCCCCATCTCGGTCGCCttt                                                                         <  1:4772611/101‑1 (MQ=255)
                         gcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTg                                                                        >  2:2493759/1‑101 (MQ=255)
                         gcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTg                                                                        <  2:8145752/101‑1 (MQ=255)
                          cccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGc                                                                       >  1:7284967/1‑101 (MQ=255)
                           ccgcGATGCAGCTTTCGGCCGCCGCCGTCTGCCTTTCCCCATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGcc                                                                      <  2:8887267/101‑1 (MQ=255)
                           ccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCTCCTTTGcc                                                                      >  1:4446893/1‑101 (MQ=255)
                           ccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGcc                                                                      <  1:5436743/101‑1 (MQ=255)
                            cgcgATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCg                                                                     <  1:6681339/101‑1 (MQ=255)
                            cgcgATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTCCCg                                                                     <  1:7200131/101‑1 (MQ=255)
                             gcgATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGa                                                                    <  2:4818330/101‑1 (MQ=255)
                               gATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAg                                                                  >  2:8238556/1‑101 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 <  1:4663270/101‑1 (MQ=255)
                                  gCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAc                                                               >  1:1015483/1‑101 (MQ=255)
                                   cAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcc                                                              >  1:2809036/1‑101 (MQ=255)
                                    aGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcct                                                             <  1:192434/101‑1 (MQ=255)
                                     gCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcctt                                                            <  2:7925532/101‑1 (MQ=255)
                                      cTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttc                                                           <  1:8039091/101‑1 (MQ=255)
                                        ttCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttcct                                                         >  1:4156433/1‑101 (MQ=255)
                                        ttCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttcct                                                         >  1:7692440/1‑101 (MQ=255)
                                        ttCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttcct                                                         <  1:5908991/101‑1 (MQ=255)
                                         tCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttcctt                                                        <  1:6886980/101‑1 (MQ=255)
                                          cGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACcttccttc                                                       <  2:2980045/101‑1 (MQ=255)
                                          cGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACcttccttc                                                       <  1:8590285/101‑1 (MQ=255)
                                           gACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCa                                                      >  1:8364803/1‑101 (MQ=255)
                                             ccgccgCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATc                                                    <  2:7751509/101‑1 (MQ=255)
                                               gccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCtt                                                  >  1:3288964/1‑101 (MQ=255)
                                                ccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTg                                                 <  2:4601443/101‑1 (MQ=255)
                                                    gATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGa                                             >  2:8423922/1‑101 (MQ=255)
                                                    gATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGa                                             >  1:8117996/1‑101 (MQ=255)
                                                     aTCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaa                                            >  2:5943875/1‑101 (MQ=255)
                                                      tCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaa                                           >  1:2965470/1‑101 (MQ=255)
                                                      tCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaa                                           <  2:3947221/101‑1 (MQ=255)
                                                       cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaa                                          <  1:3833647/101‑1 (MQ=255)
                                                         gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaag                                        >  1:5063411/1‑99 (MQ=255)
                                                         gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaag                                        <  2:6844709/101‑3 (MQ=255)
                                                          cGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..agg                                     <  2:5291555/101‑1 (MQ=255)
                                                          cGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..agg                                     >  1:2117934/1‑101 (MQ=255)
                                                           gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggg                                    >  2:676427/1‑100 (MQ=255)
                                                           gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggg                                    >  1:5877886/1‑100 (MQ=255)
                                                           gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggg                                    >  1:7534941/1‑100 (MQ=255)
                                                           gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggg                                    >  1:7807342/1‑100 (MQ=255)
                                                             ttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggt                                  >  1:7396174/1‑98 (MQ=255)
                                                              gccACGTCGCTATCGATGTTGCCCGCAGAGGTCGAGAGCACCAGCCCCATCTCTGGTGCCTTGGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggtt                                 <  2:2127835/100‑5 (MQ=255)
                                                               ccACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttg                                <  1:7064823/101‑6 (MQ=255)
                                                                cacaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttgg                               >  2:4675522/1‑95 (MQ=255)
                                                                  caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtg                             >  2:3466336/1‑100 (MQ=255)
                                                                  caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtg                             >  2:5036516/1‑100 (MQ=255)
                                                                  caTCGCCATCGATCGTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtg                             <  2:5256090/101‑2 (MQ=255)
                                                                   aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgt                            <  1:5237214/101‑3 (MQ=255)
                                                                    tCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtc                           <  2:8763681/101‑4 (MQ=255)
                                                                      gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcgg                         >  2:6203201/1‑96 (MQ=255)
                                                                       ccATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggt                        <  1:1560571/101‑7 (MQ=255)
                                                                        cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtc                       >  2:1021460/1‑94 (MQ=255)
                                                                        cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtc                       <  2:7297319/101‑8 (MQ=255)
                                                                          tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtctt                     <  2:6160973/101‑10 (MQ=255)
                                                                             aTCTTGCCCGCACAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggt                   >  2:3379579/1‑99 (MQ=255)
                                                                              tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAGGAAAAAGGGGTTGGTGTCGGTCTt.ggtg                  >  1:6397592/1‑98 (MQ=255)
                                                                               cTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtga                 >  1:6077861/1‑97 (MQ=255)
                                                                                ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGCCGAAAAAGGGGTTGGTGTCGGTCTt.gttgat                >  1:4259950/1‑97 (MQ=255)
                                                                                ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgat                >  2:797040/1‑96 (MQ=255)
                                                                                ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgat                <  1:6208218/101‑6 (MQ=255)
                                                                                    ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcaga            <  1:2413288/101‑10 (MQ=255)
                                                                                       cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAtcagacag          >  2:2357363/1‑101 (MQ=255)
                                                                                        aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAgg         <  1:1157299/101‑1 (MQ=255)
                                                                                           ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc      <  2:6343005/101‑1 (MQ=255)
                                                                                           ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc      >  2:9139674/1‑101 (MQ=255)
                                                                                             gcGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCg    <  2:4189265/100‑1 (MQ=255)
                                                                                               gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGt  >  1:2528771/1‑101 (MQ=255)
                                                                                               gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGt  >  2:5710329/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGT  >  NC_014034/2177115‑2177305

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG