Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 2,185,975 | G→C | 7.4% | intergenic (+248/‑257) | mtlK → / → RCAP_rcc02028 | mannitol 2‑dehydrogenase/FeoA family protein |
Read alignment evidence... | ||||||||||
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seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 2,185,975 | 0 | G→C | 7.4% | 2.9 ‑0.5 ‑0.4 | 67 | intergenic (+248/‑257) | mtlK/RCAP_rcc02028 | mannitol 2‑dehydrogenase/FeoA family protein |
Reads supporting (aligned to +/- strand): new base (0/5): ref base (11/51): total (11/56) | ||||||||||
Fisher's exact test strand distribution p-value = 5.82e-01 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.97e-01 |
AAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..CCC..G.CCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAG > NC_014034/2185875‑2186068
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aaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..g > 2:882043/1‑101 (MQ=255)
aaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCTCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..g < 2:6157796/101‑1 (MQ=255)
agcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..c < 1:3349982/101‑1 (MQ=255)
agcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGGCCGGGGGCGG..A..G..c > 2:4708284/1‑101 (MQ=38)
agcagcGGCTTGCCCCGAAGCCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..c < 1:7217022/101‑1 (MQ=37)
tcaggtgCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cc < 1:2980912/95‑1 (MQ=37)
cagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..ccc > 2:8215895/1‑101 (MQ=255)
cagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..ccc > 1:8901137/1‑101 (MQ=255)
cagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..ccc > 1:8211260/1‑101 (MQ=255)
cagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..ccc > 1:767092/1‑101 (MQ=255)
cgcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAGCGCCGAACCGCCCC.CTTTCACCTTGGCTCAGATATCCCGGGGGTCCGGGGGCGG..A..G..cccc < 2:7536884/100‑2 (MQ=37)
agcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccc < 2:753010/101‑2 (MQ=37)
agcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccc > 1:5106648/1‑100 (MQ=37)
agcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGGCCGGGGGCGG..A..G..cccc > 2:2354475/1‑100 (MQ=37)
agcGGCTTGCCCCGAAGGCCTTG.TATTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccc > 2:4374812/1‑100 (MQ=37)
gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..ccccc < 2:2869756/101‑3 (MQ=37)
gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..ccccc < 1:7532949/101‑3 (MQ=37)
gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..ccccc < 1:587127/101‑3 (MQ=37)
gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..ccccc < 2:5437737/101‑3 (MQ=37)
gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..ccccc < 2:6178695/101‑3 (MQ=37)
gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..ccccc < 1:1773115/101‑3 (MQ=37)
cGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccg < 1:8844289/101‑4 (MQ=37)
cGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccg < 2:1656953/101‑4 (MQ=37)
cGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccg < 2:472920/101‑4 (MQ=37)
cGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccg < 2:8389981/101‑4 (MQ=37)
ggCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccgg < 1:8015490/101‑5 (MQ=25)
gCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccggc > 2:6016404/1‑96 (MQ=25)
ttGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCGCCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccggcgg > 2:1629877/1‑94 (MQ=25)
tGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccggcggg > 2:1883161/1‑93 (MQ=21)
gCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccggcgggg > 1:554025/1‑92 (MQ=21)
gCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccggcgggg > 2:2098289/1‑92 (MQ=21)
gCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccggcgggg > 2:1882937/1‑92 (MQ=21)
ccccGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccggcgggtc < 1:7356805/101‑11 (MQ=21)
ccccGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..cccccggcgggtc < 2:6479185/101‑11 (MQ=21)
gAACCGCCGACCCCCCCC.CTTTCCCCTGGGCTAAAATCTCCGGGGGGTCCGGGGGCGG..ACCC..CCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAacac < 1:5525199/101‑1 (MQ=0)
aaCGCCGAACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAccc < 1:1118319/101‑1 (MQ=2)
aCGCCGACCCGCCCC.CTTTCGCCTTGGCTCAAATATCCGGGGGGTCGGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc < 1:1116316/101‑1 (MQ=2)
aCGCCGACCCGCCCC.CTTTCCCCTGGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc < 2:1511626/101‑1 (MQ=2)
aCGCCGACCCCCCCC.CTTTCCCCTTGGCTCAAATATCCGGGGGGTCGGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc < 1:2773655/101‑1 (MQ=2)
cGCCGAACCGCCCC.CTTTCACCTGGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCa < 2:4988315/101‑1 (MQ=2)
gAACCGCCCC.CTTCCACCTTGGCTCAAATATCCCGGGGCTCCGGGGCCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAgcgc < 2:2687490/101‑1 (MQ=2)
acccgcccc.cTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGcc < 1:6808976/95‑1 (MQ=0)
acccccccc.cTTTCCCCTTGGCTCAAATACCCGGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGcc < 1:6858652/95‑1 (MQ=0)
ccgcccc.cTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCac < 2:6501987/97‑1 (MQ=2)
cgcccc.cTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCacc < 1:2895984/98‑1 (MQ=2)
cccccc.cTTCCACCTTGGCTCAAATACCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCacc < 2:8665780/98‑1 (MQ=2)
cccc.cTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccga < 2:1703336/100‑1 (MQ=9)
ccc.cTTCCCCCTTGGCTCAAATACCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgac < 1:8501997/101‑1 (MQ=2)
ccccgctccccTTGTCTCGAATCCCCCGGTGCTCCGGGGGCGC..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgac < 2:7349847/92‑1 (MQ=2)
cTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCAccgaccga < 2:4852213/101‑1 (MQ=14)
ttgcaccggggCTCAAATCTCCGGGGGGTCGGGGGGCGG..A..G..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACcgaccgac < 1:2710784/92‑1 (MQ=2)
tccccTGGGCTCAAATACCCGGGGGGCCGGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACtt < 1:871499/98‑1 (MQ=2)
tCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACtt < 2:2158383/101‑1 (MQ=14)
tCACCTGGCCACAAATACCCGGGGGGCCGGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACtt < 2:519103/101‑1 (MQ=2)
cACCTGGGCTAAAATACCCCGGGGGTCCGGGGGCGG..C..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTg < 2:4622402/101‑1 (MQ=9)
cccTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGc < 2:5545285/100‑1 (MQ=14)
cccTTGGCTCAAATACCCGGGGGGTCGGGGGGCGG..AC.C..CCC..GTGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGc < 1:423312/100‑1 (MQ=9)
ccTGGGCTCAAATCGCCGGGGGGTCGGGGGGCGG..A..C..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCg < 1:8796984/101‑1 (MQ=9)
ccTGGGCTCAAATACCCGGGGGGCCGGGGGGCGG..A..C..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCg < 1:2354542/101‑1 (MQ=9)
ttGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGAc < 1:8669472/101‑1 (MQ=14)
ttGGCTAAAATACCCCGGGGGCCGGGGGGCGGCCC..C..CCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGAc < 1:780946/101‑1 (MQ=9)
tGGCTCAAATACCCCGGGGGTCCGGGGGCGG..ACCC..CCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACg < 1:1615606/101‑1 (MQ=9)
cactaaTACCCCGGGGGTCCGGTGTCGC..ACCC..CCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGcc < 1:2585898/97‑1 (MQ=9)
cTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..CCC..C.CGGCGGGGGGCCCAGGGGCAAGCCCGCGGCGAAACACCCCCCCGCCCCCGCCCCACCCGCGCCGGcc > 2:2888435/1‑101 (MQ=2)
cTCAAATACCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGcc < 2:5337728/101‑1 (MQ=9)
aatcgcccGGGGGCCGGGGGGCGG..G..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTc < 1:1925722/96‑1 (MQ=9)
aaaacccggggggTCCGGGGGGGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTc < 2:767079/93‑1 (MQ=9)
aaTACCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTc < 2:5698457/101‑1 (MQ=12)
atatCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTcg < 2:5877156/101‑1 (MQ=17)
atagCCCGGGGGTCGGGGGGCGG..G..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTcg < 1:9071844/101‑1 (MQ=12)
taccccGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTcgc < 2:8807057/98‑1 (MQ=12)
cccGGGGGTCCGGGGGCGG..ACCC..CCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCa < 2:9070155/101‑1 (MQ=16)
ggggggCCGGGGGGCGG..C..G..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaa < 1:4788684/100‑1 (MQ=12)
gggggTCGGGGGGCGG..G..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaaa < 1:7088895/101‑1 (MQ=12)
gggggTCGGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaaa < 2:5106479/101‑1 (MQ=12)
gggggCCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaaa < 2:8900731/101‑1 (MQ=12)
gggcgTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaaa < 2:8759791/101‑1 (MQ=12)
ggggTCCGGGGGGGG..A..G..CCC..C.CGGCGGGTCGTCTCGGGCAAAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCAGTCGCGCAAAAg < 1:6591085/101‑1 (MQ=12)
ggggTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg < 2:7728329/101‑1 (MQ=18)
ggggTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg < 2:2747861/101‑1 (MQ=18)
ggggTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg < 2:9057280/101‑1 (MQ=18)
gggTCCGGGGGCGG..A..G..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGc < 2:3394380/101‑1 (MQ=18)
ggcccGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGcc < 2:379800/98‑1 (MQ=12)
cGGGGGCGG..C..G..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGc < 2:671107/101‑1 (MQ=16)
gggggCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGcc < 2:7767353/101‑1 (MQ=18)
gggggCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGcc < 2:8210911/101‑1 (MQ=18)
gggggCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGcc < 2:554015/101‑1 (MQ=18)
gggCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcag < 2:7447771/101‑1 (MQ=18)
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AAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..CCC..G.CCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAG > NC_014034/2185875‑2186068
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 14 ≤
ATCG
< 24 ≤
ATCG
< 33 ≤
ATCG
< 40 ≤
ATCG
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