Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,185,992 C→G 100% intergenic (+265/‑240) mtlK → / → RCAP_rcc02028 mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,185,9920C→G100.0% 119.5 68intergenic (+265/‑240)mtlK/RCAP_rcc02028mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (0/67):  ref base (0/0):  total (0/68)

GAAACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..CCC..G.CCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGG  >  NC_014034/2185917‑2186077
                                                                                     |                                                                                     
gAACCGCCGACCCCCCCCCTTTCCCCTGGGCTAAAATCTCCGGGGGGTCCGGGGGCGG..ACCC..CCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAacac                                                               <  1:5525199/101‑1 (MQ=0)
  aaCGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAccc                                                             <  1:1118319/101‑1 (MQ=2)
   aCGCCGACCCGCCCCCTTTCGCCTTGGCTCAAATATCCGGGGGGTCGGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                            <  1:1116316/101‑1 (MQ=2)
   aCGCCGACCCGCCCCCTTTCCCCTGGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                            <  2:1511626/101‑1 (MQ=2)
   aCGCCGACCCCCCCCCTTTCCCCTTGGCTCAAATATCCGGGGGGTCGGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                            <  1:2773655/101‑1 (MQ=2)
    cGCCGAACCGCCCCCTTTCACCTGGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCa                                                           <  2:4988315/101‑1 (MQ=2)
        gAACCGCCCCCTTCCACCTTGGCTCAAATATCCCGGGGCTCCGGGGCCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAgcgc                                                       <  2:2687490/101‑1 (MQ=2)
         acccgcccccTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGcc                                                      <  1:6808976/95‑1 (MQ=0)
         acccccccccTTTCCCCTTGGCTCAAATACCCGGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGcc                                                      <  1:6858652/95‑1 (MQ=0)
           ccgcccccTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCac                                                    <  2:6501987/97‑1 (MQ=2)
            cgcccccTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCacc                                                   <  1:2895984/98‑1 (MQ=2)
            cccccccTTCCACCTTGGCTCAAATACCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCacc                                                   <  2:8665780/98‑1 (MQ=2)
              cccccTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccga                                                 <  2:1703336/100‑1 (MQ=9)
               ccccgctccccTTGTCTCGAATCCCCCGGTGCTCCGGGGGCGC..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgac                                                <  2:7349847/92‑1 (MQ=2)
               ccccTTCCCCCTTGGCTCAAATACCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgac                                                <  1:8501997/101‑1 (MQ=2)
                  cTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCAccgaccga                                             <  2:4852213/101‑1 (MQ=14)
                   ttgcaccggggCTCAAATCTCCGGGGGGTCGGGGGGCGG..A..G..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACcgaccgac                                            <  1:2710784/92‑1 (MQ=2)
                     tccccTGGGCTCAAATACCCGGGGGGCCGGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACtt                                          <  1:871499/98‑1 (MQ=2)
                     tCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACtt                                          <  2:2158383/101‑1 (MQ=14)
                     tCACCTGGCCACAAATACCCGGGGGGCCGGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACtt                                          <  2:519103/101‑1 (MQ=2)
                      cACCTGGGCTAAAATACCCCGGGGGTCCGGGGGCGG..C..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTg                                         <  2:4622402/101‑1 (MQ=9)
                       cccTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGc                                        <  2:5545285/100‑1 (MQ=14)
                       cccTTGGCTCAAATACCCGGGGGGTCGGGGGGCGG..AC.C..CCC..GTGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGc                                        <  1:423312/100‑1 (MQ=9)
                        ccTGGGCTCAAATCGCCGGGGGGTCGGGGGGCGG..A..C..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCg                                       <  1:8796984/101‑1 (MQ=9)
                        ccTGGGCTCAAATACCCGGGGGGCCGGGGGGCGG..A..C..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCg                                       <  1:2354542/101‑1 (MQ=9)
                          ttGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGAc                                     <  1:8669472/101‑1 (MQ=14)
                          ttGGCTAAAATACCCCGGGGGCCGGGGGGCGGCCC..C..CCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGAc                                     <  1:780946/101‑1 (MQ=9)
                           tGGCTCAAATACCCCGGGGGTCCGGGGGCGG..ACCC..CCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACg                                    <  1:1615606/101‑1 (MQ=9)
                              cactaaTACCCCGGGGGTCCGGTGTCGC..ACCC..CCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGcc                                 <  1:2585898/97‑1 (MQ=9)
                              cTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..CCC..C.CGGCGGGGGGCCCAGGGGCAAGCCCGCGGCGAAACACCCCCCCGCCCCCGCCCCACCCGCGCCGGcc                                 >  2:2888435/1‑101 (MQ=2)
                              cTCAAATACCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGcc                                 <  2:5337728/101‑1 (MQ=9)
                                  aatcgcccGGGGGCCGGGGGGCGG..G..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTc                             <  1:1925722/96‑1 (MQ=9)
                                  aaaacccggggggTCCGGGGGGGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTc                             <  2:767079/93‑1 (MQ=9)
                                  aaTACCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTc                             <  2:5698457/101‑1 (MQ=12)
                                   atatCCCGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTcg                            <  2:5877156/101‑1 (MQ=17)
                                   atagCCCGGGGGTCGGGGGGCGG..G..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTcg                            <  1:9071844/101‑1 (MQ=12)
                                    taccccGGGGGTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTcgc                           <  2:8807057/98‑1 (MQ=12)
                                       cccGGGGGTCCGGGGGCGG..ACCC..CCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCa                        <  2:9070155/101‑1 (MQ=16)
                                         ggggggCCGGGGGGCGG..C..G..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaa                      <  1:4788684/100‑1 (MQ=12)
                                          gggggTCGGGGGGCGG..G..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaaa                     <  1:7088895/101‑1 (MQ=12)
                                          gggggTCGGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaaa                     <  2:5106479/101‑1 (MQ=12)
                                          gggggCCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaaa                     <  2:8900731/101‑1 (MQ=12)
                                          gggcgTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaaa                     <  2:8759791/101‑1 (MQ=12)
                                           ggggTCCGGGGGGGG..A..G..CCC..C.CGGCGGGTCGTCTCGGGCAAAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCAGTCGCGCAAAAg                    <  1:6591085/101‑1 (MQ=12)
                                           ggggTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg                    <  2:7728329/101‑1 (MQ=18)
                                           ggggTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg                    <  2:2747861/101‑1 (MQ=18)
                                           ggggTCCGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg                    <  2:9057280/101‑1 (MQ=18)
                                            gggTCCGGGGGCGG..A..G..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGc                   <  2:3394380/101‑1 (MQ=18)
                                             ggcccGGGGGCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGcc                  <  2:379800/98‑1 (MQ=12)
                                                 cGGGGGCGG..C..G..CCCCGG.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGc              <  2:671107/101‑1 (MQ=16)
                                                  gggggCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGcc             <  2:554015/101‑1 (MQ=18)
                                                  gggggCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGcc             <  2:8210911/101‑1 (MQ=18)
                                                  gggggCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGcc             <  2:7767353/101‑1 (MQ=18)
                                                    gggCGG..A..GCCCCC..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcag           <  2:7447771/101‑1 (MQ=18)
                                                        gggcggccccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcag           <  2:6215482/91‑1 (MQ=21)
                                                         ggcggagccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcagg          <  2:406202/92‑1 (MQ=18)
                                                          ggggcgccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggc         <  1:1013457/93‑1 (MQ=21)
                                                          gcggagccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggc         <  2:5955567/93‑1 (MQ=21)
                                                          gcggagccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggc         <  2:1271743/93‑1 (MQ=21)
                                                          gcggagccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggc         <  1:33581/93‑1 (MQ=21)
                                                           ggggcccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggca        <  2:5714002/94‑1 (MQ=21)
                                                           cggcgccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggca        <  2:4798013/94‑1 (MQ=21)
                                                             gggccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggcagg      <  2:6331111/96‑1 (MQ=21)
                                                             ggcccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggcagg      <  2:3256797/96‑1 (MQ=21)
                                                             gagccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCtaggcagg      <  2:5813620/96‑1 (MQ=21)
                                                             gagccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggcagg      <  1:1608521/96‑1 (MQ=21)
                                                               gccccc..G.CCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGAc    <  2:8710564/98‑1 (MQ=25)
                                                               cccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGAc    <  2:7159399/98‑1 (MQ=25)
                                                                ccccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACg   <  1:5955563/99‑1 (MQ=25)
                                                                 cccc..G.GCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACgg  <  1:1882997/100‑1 (MQ=25)
                                                                                     |                                                                                     
GAAACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGG..A..G..CCC..G.CCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGG  >  NC_014034/2185917‑2186077

Base quality scores:  ATCG  < 3 ≤  ATCG  < 14 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG