BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,249,332	G→T	2.5%	intergenic (‑128/‑177)	parE ← / → RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,249,332	0	G→T	2.5%	15.5 ‑2.0 ‑1.8	443	intergenic (‑128/‑177)	parE/RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand): new base (8/3): ref base (209/223): total (217/226)
Fisher's exact test strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.65e-02

Only 100 of 483 total aligned reads displayed.


ATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTC  >  NC_014034/2249233‑2249431


aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACGCCCCCGGGATATTTGAGGCAAGATGAAggggg                                                                                                       >  1:4668825/1‑101 (MQ=255)


  gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGc                                                                                                     <  2:3261189/101‑1 (MQ=255)


      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                                                                                 >  2:4743120/1‑101 (MQ=255)


      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                                                                                 <  2:748514/101‑1 (MQ=255)


      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                                                                                 <  2:1571314/101‑1 (MQ=255)


      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                                                                                 <  1:6971091/101‑1 (MQ=255)


       gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCa                                                                                                <  1:4323824/101‑1 (MQ=255)


        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCAt                                                                                               <  1:93810/101‑1 (MQ=255)


          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                                                                             <  1:4907456/101‑1 (MQ=255)


          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                                                                             <  2:6423256/101‑1 (MQ=255)


           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCtt                                                                                            <  1:5339678/101‑1 (MQ=255)


           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCtt                                                                                            <  1:8895606/101‑1 (MQ=255)


           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCtt                                                                                            <  1:8369860/101‑1 (MQ=255)


           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCtt                                                                                            <  1:7445694/101‑1 (MQ=255)


           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCtt                                                                                            <  1:3066613/101‑1 (MQ=255)


           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCCGGATATTTGAGGCAAGATGAAGGGGGGGGTTCATcct                                                                                            >  1:1751418/1‑99 (MQ=25)


            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTg                                                                                           <  1:7712142/101‑1 (MQ=255)


             gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGc                                                                                          <  1:3784828/101‑1 (MQ=255)


              cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGcc                                                                                         >  2:7629550/1‑101 (MQ=255)


              cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGcc                                                                                         <  2:8539948/101‑1 (MQ=255)


               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCg                                                                                        >  1:2085417/1‑101 (MQ=255)


                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCgca                                                                                      >  2:7852134/1‑99 (MQ=255)


                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaa                                                                                      >  1:248346/1‑101 (MQ=255)


                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaa                                                                                      >  1:1336262/1‑101 (MQ=255)


                     ggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAata                                                                                  <  2:8135876/101‑1 (MQ=255)


                      gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAatat                                                                                 <  1:3973491/101‑1 (MQ=255)


                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATc                                                                                <  2:8818152/101‑1 (MQ=255)


                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATc                                                                                <  1:7930419/101‑1 (MQ=255)


                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATc                                                                                <  1:879592/101‑1 (MQ=255)


                        aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATcc                                                                               >  1:1798221/1‑101 (MQ=255)


                         cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATccc                                                                              <  1:1004950/101‑1 (MQ=255)


                          ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCg                                                                             <  1:162261/101‑1 (MQ=255)


                          ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCg                                                                             >  2:256920/1‑101 (MQ=255)


                           cGGGTCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                                                            <  2:1065071/101‑1 (MQ=255)


                            ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCggg                                                                           >  1:1523783/1‑101 (MQ=255)


                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                      >  1:3438051/1‑99 (MQ=255)


                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                      >  1:9048861/1‑101 (MQ=255)


                                  agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                     >  1:8191813/1‑101 (MQ=255)


                                  agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                     >  1:4436970/1‑101 (MQ=255)


                                  agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                     <  1:5549844/101‑1 (MQ=255)


                                   gaatGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                    <  2:7776902/101‑1 (MQ=255)


                                   gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                    <  2:2343321/101‑1 (MQ=255)


                                    aagTGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                                   <  2:5672356/101‑1 (MQ=255)


                                    aagGGGGCTCTGCCCCCGTCCCGTGCCGGGGCTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                                   <  1:7267913/101‑1 (MQ=255)


                                    aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                                   >  2:9107635/1‑101 (MQ=255)


                                      gggggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATgg                                                                 <  1:47356/101‑1 (MQ=255)


                                       ggggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                >  2:8437674/1‑101 (MQ=255)


                                         ggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGAGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATAgcgc                                                              >  2:4211706/1‑101 (MQ=37)


                                          gCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa                                                             >  2:5290302/1‑101 (MQ=255)


                                           ctctGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                                                            <  1:3544508/101‑1 (MQ=255)


                                           ctctGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                                                            <  1:2844391/101‑1 (MQ=255)


                                             ctgcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                                          >  1:7867319/5‑101 (MQ=255)


                                               gcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                                                        >  2:6671115/3‑101 (MQ=255)


                                               gcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                                                        >  1:2691587/3‑101 (MQ=255)


                                               gcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                                                        >  1:5875644/3‑101 (MQ=255)


                                               gcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                                                        >  2:3917124/3‑101 (MQ=255)


                                               gcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAATGGCGCAGCCag                                                        >  2:2172051/3‑101 (MQ=255)


                                                 ccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCagag                                                      <  2:6063452/101‑1 (MQ=255)


                                                 ccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCagag                                                      <  2:5535179/101‑1 (MQ=255)


                                                     gtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAggggg                                                  >  2:9158144/1‑101 (MQ=255)


                                                      tcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGCTGAAGGGGGGCTTTCATCTTGCCGCAAATATCCCGGGGGGGGAATGGCGCCGCCCGAgggggg                                                 >  1:2967431/1‑101 (MQ=37)


                                                      tcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                                                 >  2:537554/1‑101 (MQ=255)


                                                       cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                                                <  2:4567622/101‑1 (MQ=255)


                                                       cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                                                <  2:6314784/101‑1 (MQ=255)


                                                       cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                                                >  1:5049873/1‑101 (MQ=255)


                                                        ccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa                                               <  2:1900228/101‑1 (MQ=255)


                                                          gtGCCAGGACTCCCGCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg.c                                            <  2:1261338/101‑1 (MQ=255)


                                                          gggCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg.c                                            <  1:7629877/99‑1 (MQ=255)


                                                           tGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg.cg                                           <  1:1165148/101‑1 (MQ=255)


                                                           tGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATGGCGCAGCCAGAGGGGGGCAg.cg                                           >  2:3533680/1‑101 (MQ=255)


                                                             ccGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.cc                                        >  2:7204361/1‑101 (MQ=255)


                                                              cGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc                                       <  1:7664704/101‑1 (MQ=255)


                                                              cGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccc                                       >  2:1818101/1‑101 (MQ=255)


                                                               gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.cccc                                      >  2:3002039/1‑101 (MQ=255)


                                                               gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.cccc                                      <  1:6049399/101‑1 (MQ=255)


                                                               gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.cccc                                      <  1:226673/101‑1 (MQ=255)


                                                               gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.cccc                                      <  1:6355631/101‑1 (MQ=255)


                                                                 gACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.cccccc                                    >  2:1151446/1‑100 (MQ=255)


                                                                    tCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccccccttc                                 <  2:3702144/101‑5 (MQ=255)


                                                                    tCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccccccttc                                 <  2:2771868/101‑5 (MQ=255)


                                                                     cccccGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.ccccccttcg                                <  2:1464103/101‑6 (MQ=255)


                                                                         cGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCANCCAGAGGGGGGCAG.CGCCCCCCTTCgcggg                             >  1:655564/1‑101 (MQ=255)


                                                                                tttGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTAGCGGGCGGCcgc                      >  1:6244415/1‑101 (MQ=255)


                                                                                 ttGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcgcg                     <  1:6724924/101‑1 (MQ=255)


                                                                                 ttGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCcgcg                     >  2:5853825/1‑101 (MQ=255)


                                                                                  tGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGa                    >  2:6930111/1‑101 (MQ=255)


                                                                                   gAGGCAAGATGAAGGGGGTCTTTCATCTTGCCGAAAATATCGCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAc                   <  2:9023061/101‑1 (MQ=37)


                                                                                   gAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAc                   >  2:4908152/1‑101 (MQ=255)


                                                                                     ggCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgc                 >  2:4059790/1‑101 (MQ=255)


                                                                                       cAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcgc               >  2:2054705/1‑101 (MQ=255)


                                                                                           aTGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg           >  2:5111174/1‑101 (MQ=255)


                                                                                             gAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa         >  1:3299225/1‑101 (MQ=255)


                                                                                             gAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa         >  1:8075213/1‑101 (MQ=255)


                                                                                              aaGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa        >  2:8296706/1‑101 (MQ=255)


                                                                                              aaGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa        <  1:1452888/101‑1 (MQ=255)


                                                                                               aGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc       <  2:1388783/101‑1 (MQ=255)


                                                                                               aGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc       >  1:578997/1‑101 (MQ=255)


                                                                                               aGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCCAc       >  2:7373864/1‑101 (MQ=37)


                                                                                                  ggggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg    <  1:6664534/101‑1 (MQ=255)


                                                                                                  gggcCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCCC.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg    <  1:4226183/101‑1 (MQ=37)


ATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTC  >  NC_014034/2249233‑2249431

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG