Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,347 G→C 4.5% intergenic (‑143/‑162) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3470G→C 4.5% 38.4 ‑2.2 ‑1.0447intergenic (‑143/‑162)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (14/6):  ref base (216/210):  total (231/216)
Fisher's exact test strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.02e-01

Only 100 of 476 total aligned reads displayed.

AGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446
                                                                                                           |                                                                                                        
aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                                          >  1:3333887/1‑101 (MQ=255)
 gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                                                         <  2:1445844/101‑1 (MQ=255)
 gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                                                         >  1:5883854/1‑101 (MQ=255)
  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCgca                                                                                                        >  2:7852134/1‑99 (MQ=255)
  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                                        >  2:1165130/1‑101 (MQ=255)
  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                                        >  1:248346/1‑101 (MQ=255)
    tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                                      <  1:6625029/101‑1 (MQ=255)
     gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                                     <  1:3845200/101‑1 (MQ=255)
     gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                                     <  2:3786598/101‑1 (MQ=255)
     gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                                     <  2:6608386/101‑1 (MQ=255)
      ggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAata                                                                                                    <  2:3969777/101‑1 (MQ=255)
      ggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAata                                                                                                    <  2:8135876/101‑1 (MQ=255)
       gCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                                                   <  1:5248662/101‑1 (MQ=255)
       gCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGAATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                                                   <  1:7179108/101‑1 (MQ=255)
        cACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                                  <  1:7930419/101‑1 (MQ=255)
         aCCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATcc                                                                                                 >  1:1798221/1‑101 (MQ=255)
          cccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGACAATATccc                                                                                                >  1:7347981/1‑101 (MQ=255)
          cccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                                <  1:1004950/101‑1 (MQ=255)
           ccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                               >  2:256920/1‑101 (MQ=255)
            cGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                              <  2:5689575/101‑1 (MQ=255)
            cGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                              >  1:6665859/1‑101 (MQ=255)
             ggggCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                                             <  1:7448307/101‑1 (MQ=255)
                 cAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggggg                                                                                         >  1:4395243/1‑100 (MQ=255)
                   agaagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                       <  1:20988/101‑1 (MQ=255)
                    gaagGGGGCTCTGCCCCC.GTCCCGTGCAGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                      <  1:5378764/101‑1 (MQ=255)
                       ggaggCTCCG.CCCCAGTCCCGTGCCGGAACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tgg                                                                                  <  2:775329/98‑1 (MQ=37)
                         gggCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                                                >  2:5397572/1‑101 (MQ=255)
                         gggCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGgcg                                                                                >  1:6260475/1‑101 (MQ=255)
                          ggCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                               <  1:4513281/101‑1 (MQ=255)
                              ctgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                         <  2:6044108/97‑1 (MQ=255)
                               tgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                                                        <  2:2452754/98‑1 (MQ=255)
                                gccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                       >  1:5626813/3‑101 (MQ=255)
                                gccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                       >  1:2691587/3‑101 (MQ=255)
                                gccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                       >  2:6671115/3‑101 (MQ=255)
                                gccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                       >  1:1654059/3‑101 (MQ=255)
                                gccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                       <  1:8949544/99‑1 (MQ=255)
                                gccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                       >  2:3037958/3‑101 (MQ=255)
                                gccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                       <  1:7663264/99‑1 (MQ=255)
                                 ccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                                      >  1:7602815/2‑101 (MQ=255)
                                   ccc.gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                                    <  1:4713756/101‑1 (MQ=255)
                                       gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                 >  2:2094068/1‑101 (MQ=255)
                                       gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                 >  2:9139202/1‑101 (MQ=255)
                                       gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                 >  1:3172563/1‑101 (MQ=255)
                                        tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                >  2:4126638/1‑101 (MQ=255)
                                           cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                             >  2:8447664/1‑101 (MQ=255)
                                            gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                                           >  1:9129302/1‑101 (MQ=255)
                                            gtGACGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                                           <  1:2906103/101‑1 (MQ=255)
                                             tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg                                                          <  1:1165148/101‑1 (MQ=255)
                                             caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATACCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                               <  1:6454527/96‑1 (MQ=18)
                                                cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccc                                                      >  2:1818101/1‑101 (MQ=255)
                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccc                                                     <  1:6049399/101‑1 (MQ=255)
                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccc                                                     >  1:1470428/1‑101 (MQ=255)
                                                   gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccccc                                                   >  1:4795037/1‑100 (MQ=255)
                                                     cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cccccctt                                                 <  1:1372285/101‑4 (MQ=255)
                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                                <  2:3702144/101‑5 (MQ=255)
                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                                <  2:108225/101‑5 (MQ=255)
                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                                <  2:1281900/101‑5 (MQ=255)
                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.ccccccttc                                                <  2:2771868/101‑5 (MQ=255)
                                                         ccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtg                                                  >  2:8587461/1‑100 (MQ=0)
                                                         ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTcgc                                               >  2:6221132/1‑101 (MQ=255)
                                                         ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTcgc                                               >  2:7265326/1‑101 (MQ=255)
                                                             gggATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CCCCCtgcccc                                              >  2:5283047/1‑96 (MQ=2)
                                                             gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggc                                           >  2:3383730/1‑101 (MQ=255)
                                                             gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggc                                           <  2:4394653/101‑1 (MQ=255)
                                                             gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggc                                           <  2:9048439/101‑1 (MQ=255)
                                                               gATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCgcgggcgg                                         >  1:7741240/1‑101 (MQ=255)
                                                                 tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGcc                                       <  2:5545367/101‑1 (MQ=255)
                                                                  atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGCCGGCcg                                      >  1:346550/1‑101 (MQ=255)
                                                                     tGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGa                                   >  2:6930111/1‑101 (MQ=255)
                                                                      gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAc                                  >  1:8079337/1‑101 (MQ=255)
                                                                          cAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcgc                              >  2:2054705/1‑101 (MQ=255)
                                                                             gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                           >  1:954440/1‑101 (MQ=255)
                                                                             gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                           >  2:2293595/1‑101 (MQ=255)
                                                                                gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                        <  2:8915125/101‑1 (MQ=255)
                                                                                gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                        >  2:5877176/1‑101 (MQ=255)
                                                                                 aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa                       >  2:8296706/1‑101 (MQ=255)
                                                                                 aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGCCGGCCCCCGCGCGcgccgcca                       >  1:1564224/1‑99 (MQ=21)
                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      <  2:5049705/101‑1 (MQ=255)
                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      <  2:4794888/101‑1 (MQ=255)
                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      >  2:7557792/1‑101 (MQ=255)
                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      <  2:1388783/101‑1 (MQ=255)
                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      >  1:296785/1‑101 (MQ=255)
                                                                                     gaggggagtcTTTCATCGTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                        <  2:3529689/92‑1 (MQ=25)
                                                                                       ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                    <  2:7194864/101‑1 (MQ=255)
                                                                                        gg..gcCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCCC.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                   <  1:4226183/101‑1 (MQ=37)
                                                                                         g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAggc                  >  1:5012030/1‑100 (MQ=255)
                                                                                            ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                 >  2:6327927/1‑101 (MQ=255)
                                                                                               tttCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg              >  1:6856556/1‑101 (MQ=255)
                                                                                                  cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc           >  1:4006938/1‑101 (MQ=255)
                                                                                                  cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc           >  1:5795814/1‑101 (MQ=255)
                                                                                                   aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc          <  1:3609032/101‑1 (MQ=255)
                                                                                                   aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc          <  1:7891971/101‑1 (MQ=255)
                                                                                                    tCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCt         <  2:1574569/101‑1 (MQ=255)
                                                                                                     cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt        <  1:2324341/101‑1 (MQ=255)
                                                                                                     cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt        >  1:826460/1‑101 (MQ=255)
                                                                                                     cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt        >  2:4695158/1‑101 (MQ=255)
                                                                                                     cTTACCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGTGGCCGCGACGCGAGCCGCAACAAGGCGTGCCCCtt        >  1:8804130/1‑101 (MQ=25)
                                                                                                      ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt       <  1:8448028/101‑1 (MQ=255)
                                                                                                       tGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg      <  2:7402025/101‑1 (MQ=255)
                                                                                                         ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc    <  2:1967814/101‑1 (MQ=255)
                                                                                                           |                                                                                                        
AGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG