Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,373 C→A 4.2% intergenic (‑169/‑136) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3730C→A 4.2% 34.2 ‑2.2 ‑0.3459intergenic (‑169/‑136)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (14/6):  ref base (226/212):  total (241/218)
Fisher's exact test strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.15e-01

Only 100 of 505 total aligned reads displayed.

GGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGG  >  NC_014034/2249274‑2249472
                                                                                                             |                                                                                                       
ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GAG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TAgcgc                                                                                                         >  2:4211706/1‑101 (MQ=37)
   tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                                                                                    <  1:5873162/96‑1 (MQ=255)
      gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                                                 >  1:2691587/3‑101 (MQ=255)
        ccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                                                               <  2:5535179/101‑1 (MQ=255)
         cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                                                              <  1:4713756/101‑1 (MQ=255)
          ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                                             <  1:3531674/101‑1 (MQ=255)
            gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                                           >  2:5720029/1‑101 (MQ=255)
            gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                                           >  2:9139202/1‑101 (MQ=255)
             tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGCTGAA...GGG..G..GGCTTTCATCTTGCCGCAAATATCCCGGGGGGGGAA.TGGCGCC..GCCCGAgggggg                                                                                          >  1:2967431/1‑101 (MQ=37)
             tcccgtGCCGGGACT.CCCCCCGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                                          <  1:7246252/101‑1 (MQ=255)
              cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                         <  2:4567622/101‑1 (MQ=255)
              cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                         >  1:1388815/1‑101 (MQ=255)
              cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                         >  1:8915531/1‑101 (MQ=255)
                 gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                                                      <  2:2198624/101‑1 (MQ=255)
                 gtGACGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                                                      <  1:2906103/101‑1 (MQ=255)
                 gggCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                                                      <  1:7629877/99‑1 (MQ=255)
                  caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGA..T..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                                         <  2:6110797/96‑1 (MQ=18)
                  caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGA..T..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                                         >  2:6516338/6‑101 (MQ=18)
                  caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGA..T..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                                         >  1:4364353/6‑101 (MQ=18)
                     cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.c                                                                                <  1:7664704/101‑1 (MQ=255)
                     cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.c                                                                                <  2:8191464/101‑1 (MQ=255)
                      gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.cc                                                                               >  2:3002039/1‑101 (MQ=255)
                      gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.cc                                                                               <  1:226673/101‑1 (MQ=255)
                          cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.cccctt                                                                           <  2:2776742/101‑4 (MQ=255)
                          cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.cccctt                                                                           <  1:2422173/101‑4 (MQ=255)
                           t.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.ccccttc                                                                          <  2:3702144/101‑5 (MQ=255)
                           t.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.ccccttc                                                                          >  1:7059912/1‑97 (MQ=255)
                           t.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTGGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.ccccttc                                                                          <  2:1735425/101‑5 (MQ=255)
                             cccccGGGATATTTGCGGGAAGATGAA...GGGTCA..TGATTTCATCTTGCCCGAAATAGCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CC.CCCt                                                                             <  1:6088224/101‑1 (MQ=2)
                             cccccGGGATATTTGCGGCAAGATGAA...GGG..GCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CC.CCCt                                                                             <  1:1892457/101‑1 (MQ=0)
                             cccccGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.ccccttcg                                                                         <  2:1464103/101‑6 (MQ=255)
                              ccccGGGATATTTGCGGCAAGATGAA...GGG..GCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CC.CCCtg                                                                            >  2:8587461/1‑100 (MQ=0)
                              ccccGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTcgc                                                                         >  2:7265326/1‑101 (MQ=255)
                              ccccGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTcgc                                                                         <  1:3276956/101‑1 (MQ=255)
                               cccGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTCTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGTGGGGCAGCGCCC.CCCTTcgcg                                                                        >  1:6446734/1‑101 (MQ=25)
                                ccGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCgcgg                                                                       >  1:7989119/1‑101 (MQ=255)
                                 cGGGATATTGGCGGCAAGATGAA...GGG..GCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CC.CCCtgccc                                                                         >  1:8802080/1‑97 (MQ=2)
                                  gggATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCgcgggc                                                                     >  2:8894636/1‑101 (MQ=255)
                                   ggATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCgcgggcg                                                                    >  1:4991200/1‑101 (MQ=255)
                                          tGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGa                                                             <  1:7902126/101‑1 (MQ=255)
                                          tGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGa                                                             >  1:965530/1‑101 (MQ=255)
                                           gAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGAc                                                            >  2:4908152/1‑101 (MQ=255)
                                            aGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGAcg                                                           <  2:2065188/101‑1 (MQ=255)
                                             ggCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGAcgc                                                          <  2:4060210/101‑1 (MQ=255)
                                              gCAAGATGAA...GGG..G..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGAcgcg                                                         <  2:2026013/101‑1 (MQ=255)
                                                aaGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGGcgcgcg                                                       >  2:6260781/1‑101 (MQ=255)
                                                 aGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACgcgcgc                                                      <  2:2340814/101‑1 (MQ=255)
                                                     gAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                                  >  1:4166559/1‑101 (MQ=255)
                                                      aa...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa                                                 >  2:6865349/1‑101 (MQ=255)
                                                      aa...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGAGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa                                                 >  2:6966749/1‑101 (MQ=255)
                                                       a...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCGGCAAc                                                >  2:7379385/1‑101 (MQ=255)
                                                       a...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                >  2:7557792/1‑101 (MQ=255)
                                                                g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAggc                                            >  2:4761170/1‑100 (MQ=255)
                                                                g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                                            >  2:8374043/1‑101 (MQ=255)
                                                                g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGAAGCGCGCCGCAACAggc                                            >  1:8282087/1‑100 (MQ=255)
                                                                   ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCCCGACGCCCGCCGCAACAGGTc                                           >  1:104517/1‑101 (MQ=25)
                                                                   ggCGTTCATCTGCCCGAAAATATCGCGGGGGGTGAA.TGGCGCA..GCGAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                           <  2:1852102/101‑1 (MQ=37)
                                                                      tttCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                        >  1:6856556/1‑101 (MQ=255)
                                                                         cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                     >  1:4006938/1‑101 (MQ=255)
                                                                          aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                    >  2:5970751/1‑101 (MQ=255)
                                                                          aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                    >  2:3504687/1‑101 (MQ=255)
                                                                            cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                  >  2:4695158/1‑101 (MQ=255)
                                                                              tGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                                >  2:1101625/1‑101 (MQ=255)
                                                                                cggAAAATATCGCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                              <  2:3291633/99‑1 (MQ=255)
                                                                                ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCTTGCCCCTTTGTc                              <  1:6630177/101‑1 (MQ=255)
                                                                                ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                              <  2:1336230/101‑1 (MQ=255)
                                                                                ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGCGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                              >  2:545694/1‑101 (MQ=255)
                                                                                 cGAAAATATCCAGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTGCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                             <  2:248343/101‑1 (MQ=37)
                                                                                    aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                          <  2:2666988/101‑1 (MQ=255)
                                                                                    aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                          >  1:8511560/1‑101 (MQ=255)
                                                                                    aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                          <  1:7456494/101‑1 (MQ=255)
                                                                                     aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                         <  1:168561/101‑1 (MQ=255)
                                                                                      atagCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                        <  1:7729471/101‑1 (MQ=255)
                                                                                        atCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                      >  2:3852407/1‑101 (MQ=255)
                                                                                           ccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCa                   >  1:1193650/1‑101 (MQ=255)
                                                                                              gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                >  1:5520198/1‑101 (MQ=255)
                                                                                               gggtctgcagggCTCA..GCCGGAGGGGGGCGGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                <  2:7689603/91‑1 (MQ=255)
                                                                                               ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCCTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa               >  2:8004797/1‑101 (MQ=255)
                                                                                                gtgTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt              <  2:5871198/99‑1 (MQ=255)
                                                                                                gggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt              >  1:3395560/1‑101 (MQ=255)
                                                                                                  gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATgc            >  2:8981637/1‑100 (MQ=255)
                                                                                                  gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt            <  2:6665599/101‑1 (MQ=255)
                                                                                                  gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt            >  1:3640337/1‑101 (MQ=255)
                                                                                                    gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa          <  2:7866986/101‑1 (MQ=255)
                                                                                                    gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa          >  1:4823683/1‑101 (MQ=255)
                                                                                                    gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa          >  2:7099653/1‑101 (MQ=255)
                                                                                                     atctGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa          <  2:5295001/98‑1 (MQ=255)
                                                                                                     aa.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt         <  1:3170149/101‑1 (MQ=255)
                                                                                                     aa.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt         <  1:5397756/101‑1 (MQ=255)
                                                                                                      a.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa        >  1:5697934/1‑101 (MQ=255)
                                                                                                      a.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa        <  2:6597307/101‑1 (MQ=255)
                                                                                                      a.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa        <  1:2049720/101‑1 (MQ=255)
                                                                                                        tGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa       >  2:5111777/1‑101 (MQ=255)
                                                                                                        tGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa       <  1:6282056/101‑1 (MQ=255)
                                                                                                         ggCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCaagat      >  1:5295185/1‑97 (MQ=255)
                                                                                                         ggCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg      <  1:9158566/101‑1 (MQ=255)
                                                                                                         ggCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg      <  1:1532694/101‑1 (MQ=255)
                                                                                                         ggCGCA..GCCAGAGGGGGGCAGCG.CCTCCCTGCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg      <  2:1532035/101‑1 (MQ=255)
                                                                                                             cA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg  <  1:3738659/101‑1 (MQ=255)
                                                                                                             cA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg  >  2:1270991/1‑101 (MQ=255)
                                                                                                             |                                                                                                       
GGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGG..G..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGG  >  NC_014034/2249274‑2249472

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG