Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,381 G→A 2.5% intergenic (‑177/‑128) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3810G→A 2.5% 16.1 ‑1.5 ‑0.0437intergenic (‑177/‑128)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (8/3):  ref base (212/214):  total (220/217)
Fisher's exact test strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.23e-01

Only 100 of 513 total aligned reads displayed.

CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCG  >  NC_014034/2249281‑2249480
                                                                                                             |                                                                                                     
ccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                                                                       <  2:6063452/101‑1 (MQ=255)
ccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                                                                       <  2:5535179/101‑1 (MQ=255)
  ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                                                     >  1:7366470/1‑101 (MQ=255)
    gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                                                   >  2:9158144/1‑101 (MQ=255)
    gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                                                   >  2:5720029/1‑101 (MQ=255)
     tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                                                  >  2:4126638/1‑101 (MQ=255)
      cccgtGCCGGGGCT.CTCCCGGGGTATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGGCGAAAAGATACCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                                 <  1:1606239/101‑1 (MQ=38)
      cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                                 >  1:8915531/1‑101 (MQ=255)
      cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                                 >  1:1793311/1‑101 (MQ=255)
      cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                                 >  1:2944100/1‑101 (MQ=255)
         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                                                              >  2:2674630/1‑101 (MQ=255)
          tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg                                                                                             <  1:5202130/101‑1 (MQ=255)
          tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg                                                                                             <  1:1165148/101‑1 (MQ=255)
          tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAgcg                                                                                             >  2:3533680/1‑101 (MQ=255)
           aagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggggg                                                                                                >  2:7429631/5‑99 (MQ=18)
             cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.c                                                                                        >  1:4960159/1‑101 (MQ=255)
             cGGGACG.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.c                                                                                        <  1:2519396/101‑1 (MQ=255)
              gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.cc                                                                                       <  1:6049399/101‑1 (MQ=255)
              gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.cc                                                                                       <  1:6355631/101‑1 (MQ=255)
                gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.cccc                                                                                     >  2:1151446/1‑100 (MQ=255)
                gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.cccc                                                                                     >  1:4795037/1‑100 (MQ=255)
                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.cccctt                                                                                   <  1:6918400/101‑4 (MQ=255)
                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc.cccctt                                                                                   >  1:56779/1‑98 (MQ=255)
                         cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..NCCAGAGGGGGGCAGCGCCC.CCCTTCgcggg                                                                              >  1:655564/1‑101 (MQ=255)
                         cGGGATATTGGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CC.CCCtgccc                                                                                 >  1:8802080/1‑97 (MQ=2)
                          gggATATTGGAGGCAAGAGGAA...GGTG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCgcgggc                                                                             <  2:1022483/101‑1 (MQ=21)
                             atatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGggcggc                                                                          >  2:3121539/1‑101 (MQ=255)
                              tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGcc                                                                         >  2:3705213/1‑101 (MQ=255)
                               atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCcg                                                                        <  2:1283134/101‑1 (MQ=255)
                               atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGCCGGCcg                                                                        >  1:346550/1‑101 (MQ=255)
                                 ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCcgcg                                                                      <  1:6724924/101‑1 (MQ=255)
                                   gAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCGCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGAc                                                                    <  2:9023061/101‑1 (MQ=37)
                                   gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGAc                                                                    >  2:4908152/1‑101 (MQ=255)
                                    aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGAcg                                                                   <  2:2065188/101‑1 (MQ=255)
                                     ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGAcgc                                                                  <  1:1319276/101‑1 (MQ=255)
                                      gCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGAcgcg                                                                 <  2:2026013/101‑1 (MQ=255)
                                          gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGcgcc                                                             >  2:719610/1‑101 (MQ=255)
                                          gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGcgcc                                                             >  2:8188126/1‑101 (MQ=255)
                                           aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGcgccg                                                            >  2:5111174/1‑101 (MQ=255)
                                             gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                                          <  2:8915125/101‑1 (MQ=255)
                                             gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                                          >  2:7758247/1‑101 (MQ=255)
                                             gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                                          >  1:4166559/1‑101 (MQ=255)
                                               a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                        <  2:4716052/101‑1 (MQ=255)
                                               a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                        <  2:1388783/101‑1 (MQ=255)
                                                     gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                                                     >  1:9201958/1‑101 (MQ=255)
                                                         ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                   <  1:7633419/101‑1 (MQ=255)
                                                         ggCGTTCATCTGCCCGAAAATATCGCGGGGGGTGAA.TGGCGCA..GCGAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                   <  2:1852102/101‑1 (MQ=37)
                                                            tttCATGTTGCCGAAAGTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                                <  2:7624217/101‑1 (MQ=37)
                                                             ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCC..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGGGGCCGCGACGCGCGCCGCACCCGGTCGTGc                                               >  1:3206857/1‑101 (MQ=25)
                                                               cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                             >  1:5795814/1‑101 (MQ=255)
                                                               cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                             >  1:4006938/1‑101 (MQ=255)
                                                                aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                            <  1:7891971/101‑1 (MQ=255)
                                                                 tCTTGCCGAAAATATCCCGGGGGGTGAA.TAGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCt                                           >  1:4656666/1‑101 (MQ=255)
                                                                  cTTGCCTAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCAACGCGCGCCGCAACAGGTCGTGCCCCtt                                          >  1:7959430/1‑101 (MQ=37)
                                                                  cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                          <  1:2324341/101‑1 (MQ=255)
                                                                  cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                          >  2:3901468/1‑101 (MQ=255)
                                                                  cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                          >  1:826460/1‑101 (MQ=255)
                                                                   ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGTCC.CCCTTCGCGGGCGGCGCCGCCGCGCGCCGCAACAGGTCGTGCCCCtct                                         >  2:5943706/1‑99 (MQ=25)
                                                                   ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                         <  1:8448028/101‑1 (MQ=255)
                                                                   ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                         <  2:8341580/101‑1 (MQ=255)
                                                                     gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                                       <  2:2202575/101‑1 (MQ=255)
                                                                      ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                      <  1:2769131/101‑1 (MQ=255)
                                                                      ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGCGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                      >  2:545694/1‑101 (MQ=255)
                                                                       cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCTCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                                     <  2:6260236/101‑1 (MQ=255)
                                                                        gAAAATATCCCGGGGGGAGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTGCGCGGGGGGCCGCCACGCGCGCCGCAACAGGGCGTCCCTCTTTGTTAt                                    >  2:6594982/1‑101 (MQ=21)
                                                                         aaaaTATCCCGGGGGGTGAA.TGACGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCGGCGACGCGGCCCGCAACCGGGCGTGCCCCGTTGTCAtt                                   >  2:7338505/1‑101 (MQ=21)
                                                                          aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                  <  1:7456494/101‑1 (MQ=255)
                                                                          aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                  >  1:2003222/1‑101 (MQ=255)
                                                                           aaTATCCCGGGGGGTGAA.TGTCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                 <  1:7204665/101‑1 (MQ=255)
                                                                              atCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                              >  2:4768171/1‑101 (MQ=255)
                                                                               tCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCg                             <  2:4592916/101‑1 (MQ=255)
                                                                                   ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                         >  1:2073559/1‑101 (MQ=255)
                                                                                    gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                        <  2:1509725/101‑1 (MQ=255)
                                                                                     ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                       >  2:7019424/1‑101 (MQ=255)
                                                                                      gtgTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                      <  2:5871198/99‑1 (MQ=255)
                                                                                       ggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                     >  2:6502219/1‑101 (MQ=255)
                                                                                        gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                    >  1:3640337/1‑101 (MQ=255)
                                                                                        gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                    >  2:2579880/1‑101 (MQ=255)
                                                                                        gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                    <  2:6665599/101‑1 (MQ=255)
                                                                                         ttggactgcgcA..TCGAGCGGGAGGGAGCG.CCGCTCGTCGGTGGGGGCCGGGAGGCGCGCCGCAACAGGTCGTTCCCCTTTTTCAATCTGTCGCGCCCATGt                    <  2:7961388/94‑1 (MQ=255)
                                                                                         tGAA.TGCCGCG..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                   <  2:6931458/101‑1 (MQ=255)
                                                                                          gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                  <  1:5365/101‑1 (MQ=255)
                                                                                           atctGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                  <  2:5295001/98‑1 (MQ=255)
                                                                                           aa.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                 <  1:1465670/101‑1 (MQ=255)
                                                                                            aagggCGCA..GCCAGAGGGGGGCCGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                 <  1:3306121/98‑1 (MQ=255)
                                                                                            a.TGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                <  2:1654012/101‑1 (MQ=255)
                                                                                              tGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa               >  1:7718633/1‑101 (MQ=255)
                                                                                              tGGCGCA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa               >  1:546793/1‑101 (MQ=255)
                                                                                               tctgcA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg              <  2:2346446/98‑1 (MQ=255)
                                                                                                   cA..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg          <  1:3738659/101‑1 (MQ=255)
                                                                                                    a..GCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGCCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc         >  1:8536939/1‑101 (MQ=255)
                                                                                                       gCCAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGcc        >  2:8505149/1‑101 (MQ=255)
                                                                                                        ccAGAGGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCg       <  2:4395117/101‑1 (MQ=255)
                                                                                                          agagGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCa     >  2:8654391/1‑101 (MQ=255)
                                                                                                          agagGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCa     >  1:3035056/1‑101 (MQ=255)
                                                                                                          agagGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCa     >  1:4298867/1‑101 (MQ=255)
                                                                                                            agGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc   <  2:3333783/101‑1 (MQ=255)
                                                                                                            agGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc   >  1:624853/1‑101 (MQ=255)
                                                                                                            agGGGGGCAGCGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc   >  1:1024218/1‑101 (MQ=255)
                                                                                                            agGGGGGCAGCGCCC.CCCTGCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc   <  2:2849224/101‑1 (MQ=255)
                                                                                                             |                                                                                                     
CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCG  >  NC_014034/2249281‑2249480

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG