BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,407,597	A→C	100%	W83G (TGG→GGG)	nifH2 ←	nitrogenase iron protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,407,597	0	A→C	100.0%	1231.3	325	W83G (TGG→GGG)	nifH2	nitrogenase iron protein
Reads supporting (aligned to +/- strand): new base (165/160): ref base (0/0): total (165/160)

Only 100 of 338 total aligned reads displayed.


GCGCAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCAGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGATG  >  NC_014034/2407499‑2407694


  gcAACTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGaaa                                                                                               >  1:7200692/1‑101 (MQ=255)


   cAGCTTTCCGGGCAGATCGCCCGCGGCGGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAc                                                                                              <  2:8167307/101‑1 (MQ=255)


   cAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAc                                                                                              >  1:7873335/1‑101 (MQ=255)


    cgCTCTTCGAGCAGATCGCCCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAAcc                                                                                             <  1:5944080/100‑1 (MQ=255)


     gCTCTTCGAGCAGATCGCCCGCGCCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCg                                                                                            <  2:1210959/101‑1 (MQ=255)


       tctTCGAGCAGATCGCCCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAc                                                                                          <  1:3763377/101‑1 (MQ=255)


       tctTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAc                                                                                          >  2:4590901/1‑101 (MQ=255)


        ctTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcg                                                                                         >  2:1832508/1‑101 (MQ=255)


        ctTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcg                                                                                         >  1:6683715/1‑101 (MQ=255)


         ttCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcgg                                                                                        >  2:6974495/1‑101 (MQ=255)


             aGCAGATCGCCCGCGGCCGAGATGCCGCGCCCGGCCCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGAcggcgaa                                                                                    <  1:5484765/101‑3 (MQ=255)


              gCAGCTCGACCGGGGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGaa                                                                                   <  1:3334614/101‑1 (MQ=255)


               cAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGaaa                                                                                  >  1:3652314/1‑101 (MQ=255)


                 gATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATg                                                                                >  1:7527408/1‑101 (MQ=255)


                 gATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCACGAAACCGACGGCGGAAATg                                                                                >  2:1523354/1‑101 (MQ=255)


                 gATCGACCGCGCCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACGGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATg                                                                                <  2:2467971/101‑1 (MQ=255)


                  aTCGCCCGCGGCCGAGGGGCCGCCCCCGGCGCACCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGt                                                                               <  1:4132614/101‑1 (MQ=255)


                  aTCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATgg                                                                               >  1:8468739/1‑100 (MQ=255)


                    ccgccGGGGCCGAGTTGCCGCCCCGGGCCCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCt                                                                             <  2:6868559/98‑1 (MQ=255)


                     gACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCtt                                                                            >  2:5197478/1‑101 (MQ=255)


                       ccGCGGCCGAGATGCCGCGCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTcc                                                                          <  2:4027355/101‑1 (MQ=255)


                       ccGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAAAGACCCCGAAACAGACGGCGGGAATGTCTTca                                                                          >  2:4265762/1‑100 (MQ=255)


                        cgcgACGGAGATGCCGCCCCCGGCGCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCa                                                                         <  2:7968904/101‑1 (MQ=255)


                         tcttccgatctGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAg                                                                        <  2:8107430/91‑1 (MQ=255)


                         gcgGCGGAGACGCCGCGCCCGGCGCAGCCCCCCCCCGGCACCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAg                                                                        <  2:2150723/101‑1 (MQ=255)


                          cgGCCGAGAGCCCCCCCCCGGCGCAGCCCCCCCCCGCCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGc                                                                       <  2:2816596/101‑1 (MQ=255)


                           ggCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCt                                                                      >  1:8733396/1‑101 (MQ=255)


                             ccGAGAGGCCGCCCCCGGCGCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTc                                                                    <  1:900222/101‑1 (MQ=255)


                              ccagggccctcgcCCGGGGAGGCCCACCCCGGGCGCCGGCCCCCCCGCCTCGATGCACAGACCGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCg                                                                   <  1:5115630/91‑1 (MQ=255)


                              cGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCg                                                                   >  2:8807098/1‑101 (MQ=255)


                              cGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCg                                                                   >  2:7839908/1‑101 (MQ=255)


                                 gaggCCGCCCCCGGCGCCGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGtt                                                                <  2:7777764/98‑1 (MQ=255)


                                  gtGCCGCCCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGttt                                                               <  1:7122666/100‑1 (MQ=255)


                                   tGCCGCCCCCGGCGCAGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTg                                                              <  2:4537389/101‑1 (MQ=255)


                                    gCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTga                                                             >  2:7143371/1‑100 (MQ=255)


                                    gCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGc                                                             >  2:7499933/1‑101 (MQ=255)


                                    gCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGc                                                             >  1:1182609/1‑101 (MQ=255)


                                    cccGCGCCGGGCGCAGCCCCCCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGc                                                             <  2:1408135/100‑1 (MQ=255)


                                     ccGCCCCGGGCCCACCCCCCCCCCGGCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCg                                                            <  1:2302948/101‑1 (MQ=255)


                                      cgccCCCGGCGCCCCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCgg                                                           <  2:5633014/101‑1 (MQ=255)


                                         gcCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGcc                                                        >  2:8597561/1‑100 (MQ=255)


                                          cccGGCCCCCCCCCCCCCCGGCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCAc                                                       <  1:2266183/101‑1 (MQ=255)


                                          cccGGCCCAGCCCCCCCCCGGCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCAc                                                       <  1:4045350/101‑1 (MQ=255)


                                           cgggCGCCGCCCCCCCCCGGCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGATACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACg                                                      <  2:4445340/99‑1 (MQ=255)


                                           cggcggcgacccCCCCGCGCCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACg                                                      <  2:8470478/92‑1 (MQ=255)


                                           ccGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACg                                                      >  1:3027112/1‑101 (MQ=255)


                                             ggCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGt                                                    >  1:4538567/1‑101 (MQ=255)


                                              gcgcCGCCCCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACCGTc                                                   <  1:8812671/101‑1 (MQ=255)


                                                cccccccCCCCCCGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcgc                                                 <  2:8399435/97‑1 (MQ=255)


                                                 cAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTcgcg                                                >  1:1069582/1‑101 (MQ=255)


                                                  aGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGGAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCgcgc                                               >  2:5929689/1‑101 (MQ=255)


                                                   gCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCa                                              >  1:5749700/1‑101 (MQ=255)


                                                     ccACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAgg                                            >  2:4165153/1‑101 (MQ=255)


                                                      cccccccGCCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGc                                           <  1:3416489/99‑1 (MQ=255)


                                                        cccccGGCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTg                                         <  1:4912386/101‑1 (MQ=255)


                                                         cccgGGCCCCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGt                                        <  2:3153303/101‑1 (MQ=255)


                                                         ccccGGCCCCGGCCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGt                                        <  2:6073518/101‑1 (MQ=255)


                                                            cgcccccGCCCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTTTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGTCTGTCCa                                     <  2:3370016/96‑1 (MQ=255)


                                                            cGGCCCCGGCCCCCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCa                                     <  2:5782665/101‑1 (MQ=255)


                                                             ggCCCCGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAg                                    <  1:4591036/101‑1 (MQ=255)


                                                             gcccccGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAg                                    <  1:3559542/97‑1 (MQ=255)


                                                              gccccGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGc                                   <  1:6830404/98‑1 (MQ=255)


                                                              gCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGc                                   >  1:4446376/1‑101 (MQ=255)


                                                                cccGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCAc                                 <  1:209197/100‑1 (MQ=255)


                                                                cccGCCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCAc                                 <  1:778229/100‑1 (MQ=255)


                                                                  cGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACgg                               >  2:7627702/1‑101 (MQ=255)


                                                                  cGGCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACgg                               >  1:1638723/1‑101 (MQ=255)


                                                                   ggCCCGCCCGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGt                              >  1:1376642/1‑101 (MQ=255)


                                                                    gcccgcccGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTg                             >  2:3052740/1‑101 (MQ=255)


                                                                    gcccccccGCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTg                             <  2:6279431/101‑1 (MQ=255)


                                                                     cccgcccgCCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTgg                            >  2:2754431/1‑101 (MQ=255)


                                                                      ccgcccgcCTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTggg                           >  1:693516/1‑101 (MQ=255)


                                                                       cgcccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGc                          <  2:6561050/101‑1 (MQ=255)


                                                                       cgcccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGc                          <  2:1977057/101‑1 (MQ=255)


                                                                        ccccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCa                         <  2:3921708/100‑1 (MQ=255)


                                                                        ccccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCa                         <  2:8357078/100‑1 (MQ=255)


                                                                          ccgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGt                       <  2:6470842/101‑1 (MQ=255)


                                                                           cgccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGtt                      <  2:8901844/101‑1 (MQ=255)


                                                                            gccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTc                     <  2:6620871/101‑1 (MQ=255)


                                                                            gccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTc                     >  1:2824911/1‑101 (MQ=255)


                                                                             ccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCt                    <  1:4688717/101‑1 (MQ=255)


                                                                             ccTCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCt                    >  2:1025409/1‑101 (MQ=255)


                                                                               tCGATGCACAGACCGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGc                  >  1:8194726/1‑101 (MQ=255)


                                                                               tCGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGc                  >  1:4789852/1‑101 (MQ=255)


                                                                                cGATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGCCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcc                 >  2:7351519/1‑101 (MQ=255)


                                                                                 gATGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGccc                >  1:4009286/1‑101 (MQ=255)


                                                                                  aTGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcccc               >  2:9099155/1‑101 (MQ=255)


                                                                                  aTGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcccc               >  1:836195/1‑101 (MQ=255)


                                                                                  aTGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCAGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGcccc               <  1:7357349/101‑1 (MQ=255)


                                                                                   tGCACAGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccg              >  1:3838495/1‑101 (MQ=255)


                                                                                     cacaGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccgcc            <  1:3132518/101‑1 (MQ=255)


                                                                                      acaGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCccgccg           >  2:3488480/1‑101 (MQ=255)


                                                                                       caGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCcgccgc          >  1:2662776/1‑101 (MQ=255)


                                                                                        aGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCcgcg         <  2:4646858/101‑1 (MQ=255)


                                                                                        aGAACGCCCCCGAAACCGACGGCGGAAATGTCTTCAATCTTCGGTTTTCGGGCGCGGGGGCGCGGGCGGCACAGCCCGGAGCGCAGTCCGGCCCACCcgcg         >  1:636174/1‑101 (MQ=255)


                                                                                         ggaCGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCgcgc        <  2:4983674/99‑1 (MQ=255)


                                                                                            cGCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAgg     <  1:659943/101‑1 (MQ=255)


                                                                                             gCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGa    >  2:3733607/1‑101 (MQ=255)


                                                                                             gCCCCCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGa    >  1:3355703/1‑101 (MQ=255)


                                                                                               cccCGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGAtg  <  1:1980362/101‑1 (MQ=255)


GCGCAGCTCTTCGAGCAGATCGACCGCGGCCGAGATGCCGCGCCCGGCGCAGCCCACCCCCGGCACCGGCCCGCCCGCCTCGATGCACAGAACGCCCCAGAAACCGACGGCGGAAATGTCTTCCAGCTTCGGTTTGCGGGCACGGTCGCGCAGGCTGTCCAGCACGGTGGGCAGTTCTGCCCCGCCGCGCAGGATG  >  NC_014034/2407499‑2407694

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG