Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,467,061 G→C 100% V29L (GTC→CTC)  RCAP_rcc02292 → signal transduction histidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,467,0610G→C100.0% 1455.7 387V29L (GTC→CTC) RCAP_rcc02292signal transduction histidine kinase
Reads supporting (aligned to +/- strand):  new base (189/198):  ref base (0/0):  total (189/198)

Only 100 of 400 total aligned reads displayed.

GGGCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGGTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGT  >  NC_014034/2466963‑2467158
                                                                                                  |                                                                                                 
gggCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTc                                                                                                 <  1:1495398/101‑1 (MQ=255)
 ggcctgaGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCa                                                                                                <  2:5821039/95‑1 (MQ=255)
 ggCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCa                                                                                                <  2:8625744/101‑1 (MQ=255)
 ggCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCa                                                                                                <  1:6944461/101‑1 (MQ=255)
   ccGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGc                                                                                              <  1:1240379/101‑1 (MQ=255)
     gCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTa                                                                                            >  1:718401/1‑101 (MQ=255)
      gaGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCACGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           <  1:8951600/100‑1 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           <  1:4108270/101‑1 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           >  2:8041379/1‑101 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           <  2:4706994/101‑1 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           <  2:851113/101‑1 (MQ=255)
       aGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATg                                                                                          <  1:2510578/101‑1 (MQ=255)
          cggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgcgc                                                                                       <  2:6870616/101‑1 (MQ=255)
             cATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGa                                                                                    <  1:9146693/101‑1 (MQ=255)
              aTGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTGTGCGCAGAc                                                                                   >  1:2929339/1‑101 (MQ=255)
               tGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAcc                                                                                  <  1:3796913/101‑1 (MQ=255)
                 aTCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCt                                                                                <  2:7918315/101‑1 (MQ=255)
                 aTCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCt                                                                                <  1:1667323/101‑1 (MQ=255)
                 aTCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCt                                                                                >  2:174551/1‑101 (MQ=255)
                  tCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTg                                                                               <  2:8234726/101‑1 (MQ=255)
                  tCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTg                                                                               <  1:6307212/101‑1 (MQ=255)
                  tCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTg                                                                               <  1:7950952/101‑1 (MQ=255)
                  tCCAGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTg                                                                               <  1:3511730/101‑1 (MQ=255)
                     ggTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGcc                                                                            >  2:2002311/1‑101 (MQ=255)
                      gTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGTTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCa                                                                           <  1:4771774/101‑1 (MQ=255)
                       tGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCAc                                                                          <  2:4498772/101‑1 (MQ=255)
                       tGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCAc                                                                          >  2:2671799/1‑101 (MQ=255)
                        ggCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCAcc                                                                         <  1:3361690/101‑1 (MQ=255)
                         gCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCg                                                                        <  1:8428492/101‑1 (MQ=255)
                           gACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGaa                                                                      <  1:749850/101‑1 (MQ=255)
                             cgcgGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACg                                                                    <  2:6653540/101‑1 (MQ=255)
                              gcgGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACgg                                                                   <  2:1715202/101‑1 (MQ=255)
                               cgGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACggg                                                                  >  2:4335954/1‑101 (MQ=255)
                               cgGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACggg                                                                  >  1:8346385/1‑101 (MQ=255)
                                 gCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgcc                                                                <  1:2780260/101‑1 (MQ=255)
                                 gCACGGGCGGTATGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgcc                                                                <  1:110977/101‑1 (MQ=255)
                                  cgcgcgCGCTCCGGGGCTTCGCCTGGCGGTGATCATGGGGGCGGTGCTGCCGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgccg                                                               <  2:4241015/96‑1 (MQ=255)
                                    cGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgccgcc                                                             <  1:3186590/101‑1 (MQ=255)
                                     gggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGccgccg                                                            <  2:7570855/101‑1 (MQ=255)
                                       gCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaa                                                          <  1:5287177/101‑1 (MQ=255)
                                       gCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaa                                                          >  1:1885160/1‑101 (MQ=255)
                                         ggTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaaaa                                                        >  2:3509676/1‑101 (MQ=255)
                                         ggTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaaaa                                                        >  2:3045781/1‑101 (MQ=255)
                                          gTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAAc                                                       <  2:2489470/101‑1 (MQ=255)
                                           tCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAAcc                                                      >  1:1387928/1‑101 (MQ=255)
                                             tGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGt                                                    >  1:4975942/1‑101 (MQ=255)
                                             tGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGt                                                    >  1:607605/1‑101 (MQ=255)
                                               gggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCt                                                  <  2:6983469/101‑1 (MQ=255)
                                                ggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTg                                                 <  1:2111319/101‑1 (MQ=255)
                                                 gCTGCGGCTGACGGTGATCATGGGGGCGTTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgc                                                >  1:6526979/1‑101 (MQ=255)
                                                 gCTGCGGCTGACGGTGATCATGGGGGCGGTGCTTACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgc                                                <  1:4228567/101‑1 (MQ=255)
                                                  cTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcg                                               >  2:7157503/1‑101 (MQ=255)
                                                    gCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcgcg                                             >  2:3853211/1‑101 (MQ=255)
                                                       gCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCgcgg                                          >  2:7262669/1‑101 (MQ=255)
                                                       gCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCgcgg                                          >  1:770604/1‑101 (MQ=255)
                                                       gCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCgcgg                                          >  2:283524/1‑101 (MQ=255)
                                                         tGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCgcggcg                                        >  1:8989682/1‑101 (MQ=255)
                                                         tGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCgcggcg                                        >  1:7630617/1‑101 (MQ=255)
                                                          gACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGa                                       >  1:8696817/1‑101 (MQ=255)
                                                              gTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCt                                   <  1:5849425/101‑1 (MQ=255)
                                                              gTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCt                                   >  2:4867360/1‑101 (MQ=255)
                                                              gTGATCATGGGGGCGGCGCTGCCGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCt                                   <  2:8956799/101‑1 (MQ=255)
                                                               tGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTg                                  <  2:1531234/101‑1 (MQ=255)
                                                                gATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTgg                                 >  2:1196466/1‑101 (MQ=255)
                                                                gATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTgg                                 >  2:2099404/1‑101 (MQ=255)
                                                                 aTCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTggg                                >  1:1994810/1‑101 (MQ=255)
                                                                  tCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGc                               >  2:3665412/1‑101 (MQ=255)
                                                                  tCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGc                               >  2:1806235/1‑101 (MQ=255)
                                                                   cATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCg                              <  1:5412146/101‑1 (MQ=255)
                                                                    aTGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCga                             >  2:6383373/1‑101 (MQ=255)
                                                                     tGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgag                            >  2:2562796/1‑101 (MQ=255)
                                                                     tGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgag                            >  2:740903/1‑101 (MQ=255)
                                                                      gggggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgagc                           >  1:3637058/1‑100 (MQ=255)
                                                                      gggggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgaga                           <  2:5444464/101‑1 (MQ=255)
                                                                      gggggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgaga                           <  2:8117309/101‑1 (MQ=255)
                                                                      gggggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgaga                           >  2:5010680/1‑101 (MQ=255)
                                                                        gggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACg                         <  2:1387896/101‑1 (MQ=255)
                                                                        gggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACg                         <  1:7416166/101‑1 (MQ=255)
                                                                          gCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCt                       <  2:3171195/101‑1 (MQ=255)
                                                                          gCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCt                       <  2:2297356/101‑1 (MQ=255)
                                                                           cGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTg                      >  1:4616133/1‑101 (MQ=255)
                                                                            ggTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGa                     <  2:1025516/101‑1 (MQ=255)
                                                                                cTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgc                 >  2:7783651/1‑101 (MQ=255)
                                                                                 tGAGGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCGTGGGCGAGACGCTGATGgcc                >  1:7231894/1‑101 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGCCCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                <  1:5505645/101‑1 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                >  1:2619814/1‑101 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                <  1:6660950/101‑1 (MQ=255)
                                                                                  gACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgccg               >  2:5290266/1‑101 (MQ=255)
                                                                                    cgccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgccgcc             <  1:5669468/101‑1 (MQ=255)
                                                                                    cgccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgccgcc             <  1:8041719/101‑1 (MQ=255)
                                                                                     gccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGccgccg            >  2:947733/1‑101 (MQ=255)
                                                                                     gccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGccgccg            >  2:5343654/1‑101 (MQ=255)
                                                                                     gccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGccgccg            >  1:242755/1‑101 (MQ=255)
                                                                                      ccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgc           >  1:5626166/1‑101 (MQ=255)
                                                                                        gcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcgc         >  2:4060677/1‑101 (MQ=255)
                                                                                           cGCCGTGCTCAGCTATGCGCGGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCgccgcgccgc      <  1:896771/101‑1 (MQ=255)
                                                                                           cGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCgccgcgccgc      >  2:5573525/1‑101 (MQ=255)
                                                                                             ccGTGCTCAGCTATGCGCAGCCCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAg    <  1:5094296/101‑1 (MQ=255)
                                                                                             ccGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAg    >  2:6125233/1‑101 (MQ=255)
                                                                                               gTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGt  >  1:1000460/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GGGCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGGTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGT  >  NC_014034/2466963‑2467158

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG