Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,470,260 G→C 3.6% intergenic (‑193/+24) RCAP_rcc02294 ← / ← proB signal transduction histidine kinase/glutamate 5‑kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,470,2600G→C 3.6% 16.8 ‑1.4 ‑0.8350intergenic (‑193/+24)RCAP_rcc02294/proBsignal transduction histidine kinase/glutamate 5‑kinase
Reads supporting (aligned to +/- strand):  new base (3/10):  ref base (142/194):  total (145/205)
Fisher's exact test strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.63e-01

Only 100 of 403 total aligned reads displayed.

GGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGATCTCGCCCG  >  NC_014034/2470162‑2470360
                                                                                                  |                                                                                                    
ggCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGCcc                                                                                                    >  1:7861049/1‑101 (MQ=255)
  cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                                 <  2:1709973/101‑1 (MQ=255)
  cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                                 <  2:3610708/101‑1 (MQ=255)
  cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                                 <  1:1744446/101‑1 (MQ=255)
    cgGGGACGTTGATTACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTCGATGAAACCAAA.GGGGGGGCCCTGcc                                                                                               >  2:8400203/1‑101 (MQ=255)
      gggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccc                                                                                             >  1:1009535/1‑101 (MQ=255)
       ggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccccc                                                                                            <  2:2720250/101‑1 (MQ=255)
        gACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccccc                                                                                           <  1:6083639/101‑1 (MQ=255)
        gACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccccc                                                                                           <  2:5463689/101‑1 (MQ=255)
        gACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccccc                                                                                           >  1:1264286/1‑101 (MQ=255)
         aCGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCct                                                                                          <  1:4821575/101‑1 (MQ=255)
         aCGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCct                                                                                          <  2:3504378/101‑1 (MQ=255)
          cGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctc                                                                                         <  1:8430799/101‑1 (MQ=255)
          cGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctc                                                                                         <  1:1118942/101‑1 (MQ=255)
          cGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctc                                                                                         <  2:2181451/101‑1 (MQ=255)
             tgatgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGc                                                                                      >  1:2952424/1‑101 (MQ=255)
             tgatgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGc                                                                                      <  2:2331696/101‑1 (MQ=255)
              gatgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAACCCCGGGCGTTGATGACACCAAA.CGGGGGGCCCTGCCCCCctacgcc                                                                                     >  2:2399376/1‑96 (MQ=255)
               atgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGccc                                                                                    <  2:3028424/101‑1 (MQ=255)
                tgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGcccc                                                                                   <  1:6383866/101‑1 (MQ=255)
                tgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGcccc                                                                                   <  1:2340173/101‑1 (MQ=255)
                      gggggCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTc                                                                             <  2:849300/99‑1 (MQ=255)
                      gcgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTc                                                                             >  1:8624616/1‑101 (MQ=255)
                       cgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCa                                                                            <  2:6356009/101‑1 (MQ=255)
                        gggCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAg                                                                           >  1:5119193/1‑101 (MQ=255)
                        gggCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAg                                                                           <  2:7620951/101‑1 (MQ=255)
                        gggCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAg                                                                           >  1:9031586/1‑101 (MQ=255)
                            gcCAAAGGAACGCGCCCGGATCAACGCCGCCGGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCCGCCCCCCTCGGCCCCGGCCTCGGCCGa                                                                       >  2:4220099/1‑101 (MQ=255)
                               aaaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCa                                                                    >  2:5077135/1‑101 (MQ=255)
                               aaaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCa                                                                    >  2:7939885/1‑101 (MQ=255)
                                 aGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAcc                                                                  <  2:7096746/101‑1 (MQ=255)
                                 aGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAcc                                                                  >  2:9046049/1‑101 (MQ=255)
                                 aGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAcc                                                                  <  1:4935103/101‑1 (MQ=255)
                                   gAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCAt                                                                <  1:7716209/101‑1 (MQ=255)
                                   gAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCAt                                                                >  2:622966/1‑101 (MQ=255)
                                    aaCGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATg                                                               >  2:5830718/1‑101 (MQ=255)
                                       gcgcCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgtcg                                                            <  1:4593659/101‑1 (MQ=255)
                                       gcgcCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgtcg                                                            <  1:2572987/101‑1 (MQ=255)
                                          cccGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGtcgtcg                                                         <  2:1145087/101‑1 (MQ=255)
                                             ggATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGa                                                      >  2:6047074/1‑101 (MQ=255)
                                             ggATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGa                                                      >  2:7168459/1‑101 (MQ=255)
                                              gATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGAt                                                     <  1:3063999/101‑1 (MQ=255)
                                              gATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGAt                                                     >  1:5437373/1‑101 (MQ=255)
                                              gATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGAt                                                     >  1:4151865/1‑101 (MQ=255)
                                               aTCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATg                                                    <  2:2076211/101‑1 (MQ=255)
                                               aTCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATg                                                    <  2:4853063/101‑1 (MQ=255)
                                               aTCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATg                                                    >  1:3579549/1‑101 (MQ=255)
                                               aTCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATg                                                    >  1:2811821/1‑101 (MQ=255)
                                                tCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGc                                                   <  2:8568037/101‑1 (MQ=255)
                                                 cAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGca                                                  >  2:5155698/1‑101 (MQ=255)
                                                  aaCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcac                                                 <  2:8285166/101‑1 (MQ=255)
                                                  aaCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcac                                                 >  1:7124011/1‑101 (MQ=255)
                                                  aaCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcac                                                 <  2:6635715/101‑1 (MQ=255)
                                                    cgccgcCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACaa                                               <  2:4389446/101‑1 (MQ=255)
                                                    cgccgcCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACaa                                               <  2:3781759/101‑1 (MQ=255)
                                                      ccgcccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgc                                             <  1:7311729/101‑1 (MQ=255)
                                                           cgcgcgCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcggc                                        <  2:3244321/101‑1 (MQ=255)
                                                               cgcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGcgcggcgcgg                                    >  1:5863778/1‑101 (MQ=255)
                                                                gcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCgcggcgcggc                                   >  2:8665681/1‑101 (MQ=255)
                                                                gcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCgcggcgcggc                                   >  1:6695925/1‑101 (MQ=255)
                                                                gcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCgcggcgcggc                                   >  1:8240160/1‑101 (MQ=255)
                                                                  aaaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGccc                                 <  1:973174/101‑1 (MQ=255)
                                                                    aCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCgg                               <  1:8869288/101‑1 (MQ=255)
                                                                     ctcGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGa                              <  2:8150235/99‑1 (MQ=255)
                                                                      ccGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGAt                             <  1:1414998/101‑1 (MQ=255)
                                                                      ccGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGAt                             <  1:2065525/101‑1 (MQ=255)
                                                                      ccGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGAt                             <  2:141525/101‑1 (MQ=255)
                                                                       cGGGTGTTGATGCAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGaag                            >  1:8150584/1‑99 (MQ=255)
                                                                        gggTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAg                           >  1:9146645/1‑101 (MQ=255)
                                                                        gggTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCCGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAg                           >  2:1046243/1‑101 (MQ=255)
                                                                         ggTGTTGATGAATCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGc                          <  2:5871779/101‑1 (MQ=255)
                                                                           tgtTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGccc                        <  2:1644852/101‑1 (MQ=255)
                                                                              tgatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagc                     <  2:8781932/101‑1 (MQ=255)
                                                                              tgatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagc                     >  2:4724366/1‑101 (MQ=255)
                                                                               gatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGTTGCACAAGCGCGGCGCGGCCCGGCTAGCCcagca                    >  2:722564/1‑101 (MQ=255)
                                                                               gatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagca                    >  2:8845934/1‑101 (MQ=255)
                                                                               gatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagca                    >  2:1314959/1‑101 (MQ=255)
                                                                               gatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagca                    >  1:7800196/1‑101 (MQ=255)
                                                                               gatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGAGGCGCGGCCCGGATAGGCcagca                    >  1:34383/1‑101 (MQ=255)
                                                                                 tgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCagcagc                  >  2:6908386/1‑101 (MQ=255)
                                                                                    aaCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGcc               >  2:4576588/1‑101 (MQ=255)
                                                                                      ccAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTc             >  1:5770572/1‑101 (MQ=255)
                                                                                      ccAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTc             <  2:2620725/101‑1 (MQ=255)
                                                                                      ccAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTc             <  1:4435580/101‑1 (MQ=255)
                                                                                      ccAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTc             <  2:7754527/101‑1 (MQ=255)
                                                                                      ccAAA.GGGGGGCCCCTGCCCCCCTCTGCCCCGGCCCCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTc             <  1:2399449/101‑1 (MQ=255)
                                                                                        caaagggggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCg            <  1:7622921/97‑1 (MQ=255)
                                                                                        caaagggggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCg            <  2:7513552/97‑1 (MQ=255)
                                                                                        caaagggggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGAACCGGCTca            >  1:6294664/5‑100 (MQ=255)
                                                                                         aaagggggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGa           >  1:5156966/4‑101 (MQ=255)
                                                                                          aagggggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGAt          >  2:4042865/3‑101 (MQ=255)
                                                                                           agggggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATCGCCCAGCAGGGCATGGAtc         >  1:4282883/2‑101 (MQ=255)
                                                                                           agggggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGAtc         <  1:7273931/100‑1 (MQ=255)
                                                                                            gggggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGAtct        >  2:3407843/1‑101 (MQ=255)
                                                                                              gggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGAtctcc      >  1:40499/1‑100 (MQ=255)
                                                                                              gggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGATCTCg      <  1:5565836/101‑1 (MQ=255)
                                                                                              gggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGATCTCg      >  1:6800723/1‑101 (MQ=255)
                                                                                               ggggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGATCTCGc     <  2:2550644/101‑1 (MQ=255)
                                                                                                gggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGATCTCGcc    <  1:5223619/101‑1 (MQ=255)
                                                                                                 ggCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGATCTCGccc   >  1:3195642/1‑101 (MQ=255)
                                                                                                  |                                                                                                    
GGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTCGATCTCGCCCG  >  NC_014034/2470162‑2470360

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG