Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,003 A→G 3.2% intergenic (+51/‑191) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0030A→G 3.2% 15.7 ‑1.1 ‑0.1340intergenic (+51/‑191)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/5):  ref base (123/206):  total (129/211)
Fisher's exact test strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.59e-01

Only 100 of 388 total aligned reads displayed.

GCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.A.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTG  >  NC_014034/2493904‑2494102
                                                                                                     |                                                                                                         
gcgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgca                                                                                                           <  1:6456884/101‑1 (MQ=25)
 cgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcag                                                                                                          <  2:1359759/101‑1 (MQ=25)
  gTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagc                                                                                                         <  2:6658635/101‑1 (MQ=25)
  gTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagc                                                                                                         <  1:4679238/101‑1 (MQ=25)
   tCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagca                                                                                                        >  1:2638230/1‑101 (MQ=25)
   tCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATATTTgcagca                                                                                                        >  2:8049699/1‑101 (MQ=25)
    cTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCaa                                                                                                       <  2:7821683/101‑1 (MQ=25)
     tGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                                                                                      <  2:7415482/101‑1 (MQ=21)
     tGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                                                                                      <  1:5166232/101‑1 (MQ=21)
     tGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                                                                                      <  2:5339795/101‑1 (MQ=21)
      ggCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGGCTCCCCCCGGGATATTTGCAGAAAGa                                                                                                     >  1:5377206/1‑101 (MQ=21)
       gCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAt                                                                                                    <  2:4721048/101‑1 (MQ=21)
       gCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAt                                                                                                    <  2:2901211/101‑1 (MQ=21)
        cccAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATg                                                                                                   <  1:3341178/101‑1 (MQ=21)
          cAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                                                                                 >  1:9181601/1‑101 (MQ=21)
           cgcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAGG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                <  2:3376977/100‑1 (MQ=18)
           aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                >  1:1291021/1‑101 (MQ=21)
            gcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                               <  1:3076599/101‑1 (MQ=21)
             cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                              <  2:3316583/101‑1 (MQ=21)
             cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                              <  2:7062458/101‑1 (MQ=21)
             cgccgcGGCGCGGGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                              <  2:4242139/101‑1 (MQ=18)
              gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                             >  1:7704678/1‑101 (MQ=21)
                  cggcggGCGCGCGGCACGACAGGCCGGGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGGAAGATGAAACCGGATg                                                                                         >  2:5961040/1‑101 (MQ=18)
                   ggcgggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                        <  2:3732290/101‑1 (MQ=21)
                     cgggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                      <  2:1576925/101‑1 (MQ=21)
                      gggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                     <  2:2294198/101‑1 (MQ=21)
                      gggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                     <  1:4058355/101‑1 (MQ=21)
                       ggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                    <  2:92579/101‑1 (MQ=21)
                       ggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                    <  1:1945162/101‑1 (MQ=21)
                       ggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                    <  1:5678555/101‑1 (MQ=21)
                        gcgcgcGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                   <  1:786711/101‑1 (MQ=21)
                        gcgcgcGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                   <  1:5943665/101‑1 (MQ=21)
                        gcgcgcGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                   <  1:3715740/101‑1 (MQ=21)
                         cgcgcgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                  >  1:4839243/1‑101 (MQ=21)
                             gggCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                              <  2:5377022/100‑1 (MQ=21)
                             cgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                              <  1:2514820/101‑1 (MQ=21)
                             cgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                              <  2:3788717/101‑1 (MQ=21)
                             cgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                              <  2:4001678/101‑1 (MQ=21)
                              ggCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                             <  1:4194395/101‑1 (MQ=21)
                              ggCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                             <  1:6153934/101‑1 (MQ=21)
                                cACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                           >  2:7296832/1‑101 (MQ=21)
                                 aCGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                          >  1:314690/1‑101 (MQ=21)
                                 aCGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                          <  2:2770154/101‑1 (MQ=21)
                                  cGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                         >  1:6788868/1‑101 (MQ=21)
                                  cGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                         <  2:2846990/101‑1 (MQ=21)
                                    aCAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                       >  2:8879047/1‑101 (MQ=21)
                                    aCAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                       <  1:3255690/101‑1 (MQ=21)
                                     cAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                      >  2:6323861/1‑101 (MQ=21)
                                     cAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                      <  1:8146757/101‑1 (MQ=21)
                                      aGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                     <  2:7585988/101‑1 (MQ=21)
                                       ggCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                    >  2:6446711/1‑101 (MQ=21)
                                       ggCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                    <  2:6865874/101‑1 (MQ=21)
                                        gCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                   <  2:2140873/101‑1 (MQ=21)
                                        gCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                   <  2:2527562/101‑1 (MQ=21)
                                         ccGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGCACTCCCCCCGGGATATTTGCAGCACGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                  <  1:6568343/101‑1 (MQ=21)
                                          cGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                 <  1:6324418/101‑1 (MQ=21)
                                          cGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                 >  1:234793/1‑101 (MQ=18)
                                          cGAGTA.AGGGGGGGGCGCTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAACCCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                 <  2:8574761/101‑1 (MQ=18)
                                           gAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                >  1:8974454/1‑101 (MQ=18)
                                              gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAAATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                              >  2:5664204/6‑101 (MQ=17)
                                               taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                             <  1:1707389/97‑1 (MQ=255)
                                                  ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAGGAAACCGGATGCCTTTCATCTTGCCCGAAATATCACggcggg                                                          >  1:8301384/2‑101 (MQ=18)
                                                   gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                         >  2:4399036/1‑100 (MQ=255)
                                                   gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                         <  2:618304/101‑1 (MQ=255)
                                                    ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                        <  1:880170/101‑1 (MQ=255)
                                                    ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                        <  1:3931415/101‑1 (MQ=255)
                                                    ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                        <  1:1154327/101‑1 (MQ=255)
                                                     gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                       <  1:1052772/101‑1 (MQ=255)
                                                       gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                    >  2:5189108/1‑101 (MQ=255)
                                                       gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                    <  2:8176758/101‑1 (MQ=255)
                                                         gcgcTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                  <  2:1947132/101‑1 (MQ=255)
                                                          cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                 <  1:3525941/101‑1 (MQ=255)
                                                             tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                           <  2:2638148/101‑2 (MQ=255)
                                                              gCCCCCCGTCCGCAAGCGGAGTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGCGGGTGAA.TGGC..G.aag                                          <  1:7251021/101‑3 (MQ=21)
                                                              gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                          <  1:984402/101‑3 (MQ=255)
                                                                ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                       >  2:5877657/1‑96 (MQ=255)
                                                                  cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                      <  2:3582138/97‑7 (MQ=255)
                                                                     gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                   <  2:1152574/100‑10 (MQ=255)
                                                                            aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCag                            <  1:6927312/101‑2 (MQ=18)
                                                                                gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGGcgcgcc                         >  2:5435998/1‑96 (MQ=18)
                                                                                gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc                      >  1:714694/1‑101 (MQ=18)
                                                                                gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc                      >  2:7126503/1‑101 (MQ=18)
                                                                                gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc                      >  2:4464824/1‑101 (MQ=17)
                                                                                  cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.cccc                    <  2:882595/101‑1 (MQ=17)
                                                                                      ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccccctg                <  1:8368439/101‑4 (MQ=17)
                                                                                       cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccccctgc               <  2:7704352/101‑5 (MQ=17)
                                                                                          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGcccc             >  2:1083124/1‑101 (MQ=18)
                                                                                           ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcg            >  1:1831706/1‑101 (MQ=2)
                                                                                           ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcg            >  1:2146677/1‑101 (MQ=2)
                                                                                           ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcg            >  2:4633388/1‑101 (MQ=18)
                                                                                            gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcgg           >  2:8980044/1‑101 (MQ=18)
                                                                                             atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcgcc          >  2:5005103/1‑99 (MQ=2)
                                                                                               atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccgg        <  1:8554535/101‑1 (MQ=2)
                                                                                               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CAAGAGGGGGGC.AGCGCCCCCCTGCCCcggcgg        >  1:4507048/1‑101 (MQ=18)
                                                                                                tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGt       >  2:3144781/1‑101 (MQ=18)
                                                                                                 ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTc      <  1:7429955/101‑1 (MQ=17)
                                                                                                 ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGAGGC.AGCGCCCCCCTGCCCCGGCGGTc      >  1:5013433/1‑101 (MQ=17)
                                                                                                 ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcggcc      >  1:8007750/1‑99 (MQ=17)
                                                                                                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.CGCGCCCCCCTGCCCcggcggccg     >  2:3906303/1‑98 (MQ=17)
                                                                                                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGCGGGGC.AGCGCCCCCCTGCCCCCGCGGTcc     >  2:1769121/1‑101 (MQ=17)
                                                                                                     |                                                                                                         
GCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.A.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTG  >  NC_014034/2493904‑2494102

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG