Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,014 A→G 3.4% intergenic (+62/‑180) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0140A→G 3.4% 16.1 ‑0.6 ‑1.3325intergenic (+62/‑180)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/5):  ref base (141/172):  total (147/178)
Fisher's exact test strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.57e-02

Only 100 of 362 total aligned reads displayed.

AGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.A.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTG  >  NC_014034/2493915‑2494113
                                                                                                     |                                                                                                        
cgcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAGG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                          <  2:3376977/100‑1 (MQ=18)
aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                          >  1:1291021/1‑101 (MQ=21)
aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                          <  1:2738025/101‑1 (MQ=21)
 gcgcCGGGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                                         <  2:8656445/101‑1 (MQ=18)
 gcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                                         >  2:7116961/1‑101 (MQ=21)
 gcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                                         <  1:3076599/101‑1 (MQ=21)
  cgccgcGGCGGGCGCGCGGCATGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                                        <  1:5745430/101‑1 (MQ=18)
  cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                                        <  2:3316583/101‑1 (MQ=21)
  cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                                        <  1:6872812/101‑1 (MQ=21)
   gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                                       <  1:1425954/101‑1 (MQ=21)
    ccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                      <  1:4852884/101‑1 (MQ=21)
    ccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                      <  2:4062327/101‑1 (MQ=21)
      gcggcgGGGGCGCGGCACGACAGGCCGGGTA.AGGGGGGGGCGCTGCGCCCCCTCCCGAAGCGGCCTTCCCCCGGGGAAGTTGCAGGACGATCAAACCGGag                                                                                                    >  2:162524/1‑100 (MQ=16)
       cggcggGCGCGCGGCACGACAGGCCGGGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGGAAGATGAAACCGGATg                                                                                                   >  2:5961040/1‑101 (MQ=18)
        ggcgggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                  <  2:3732290/101‑1 (MQ=21)
         gcgggcgCACGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                                 <  1:8601610/101‑1 (MQ=18)
          cgggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                <  2:3529329/101‑1 (MQ=21)
           gggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                               <  2:1045837/101‑1 (MQ=21)
            ggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                              <  2:92579/101‑1 (MQ=21)
             gcgcgcGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGAAACCGGCTGCCTTTc                                                                                             >  1:1802607/1‑101 (MQ=18)
             gcgcgcGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                             <  1:3715740/101‑1 (MQ=21)
             gcgcgcGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                             <  1:786711/101‑1 (MQ=21)
                  cgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                        <  2:6857187/101‑1 (MQ=21)
                  cgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                        <  1:2514820/101‑1 (MQ=21)
                   ggCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                       >  1:8177107/1‑101 (MQ=21)
                     cACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                     <  1:6018647/101‑1 (MQ=21)
                      aCGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCCTCTTGccc                                                                                    >  2:1895580/1‑101 (MQ=18)
                      aCGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                    >  1:314690/1‑101 (MQ=21)
                       cGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                   >  1:1210863/1‑101 (MQ=21)
                         aCAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                 <  1:3255690/101‑1 (MQ=21)
                          cAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                >  2:6323861/1‑101 (MQ=21)
                          cAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                <  1:8146757/101‑1 (MQ=21)
                          cAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTACCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                <  2:5231288/101‑1 (MQ=21)
                           aGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCCCAAGCGGACTCCCCCCGCGATATTTTCAGGAAGATGAAACCGGATGCCTTTCATATTGCCCGACat                                                                               >  2:8955022/1‑101 (MQ=18)
                            ggCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                              >  2:6446711/1‑101 (MQ=21)
                             gCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                             <  2:2140873/101‑1 (MQ=21)
                             gCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                             <  2:2527562/101‑1 (MQ=21)
                              ccGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGCACTCCCCCCGGGATATTTGCAGCACGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                            <  1:6568343/101‑1 (MQ=21)
                               cGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                           >  1:234793/1‑101 (MQ=18)
                               cGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                           <  1:1848598/101‑1 (MQ=18)
                               cGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                           <  1:6324418/101‑1 (MQ=21)
                                gAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                          >  1:8974454/1‑101 (MQ=18)
                                cgagtagggggggggCGCTGCCCCCCGTCCGCAAGCGGTCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                           <  2:4334386/93‑1 (MQ=18)
                                 aGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                         >  2:5415473/1‑101 (MQ=18)
                                   gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCGTTCATCTTGCCCGAAATATCCCgg                                                                        >  2:5025270/6‑101 (MQ=18)
                                    taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                       >  2:7402294/5‑101 (MQ=255)
                                    taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                       >  2:3951065/5‑101 (MQ=255)
                                       ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                    <  2:7701644/100‑1 (MQ=255)
                                        gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                   >  1:5378965/1‑100 (MQ=255)
                                         ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                  <  1:3931415/101‑1 (MQ=255)
                                         ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                  <  1:880170/101‑1 (MQ=255)
                                          gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                 <  2:6127768/101‑1 (MQ=255)
                                          gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                 <  1:1052772/101‑1 (MQ=255)
                                            gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaag                                                               >  2:1114671/1‑100 (MQ=255)
                                             ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                             <  2:3627691/101‑1 (MQ=255)
                                             ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                             >  1:9133148/1‑101 (MQ=255)
                                               cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                           >  1:734792/1‑101 (MQ=255)
                                               cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                           <  1:3525941/101‑1 (MQ=255)
                                               cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                           >  1:4154401/1‑101 (MQ=255)
                                               cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGc                                                           >  1:6159433/1‑101 (MQ=255)
                                                gcTGCCCCCCGTCCGCAAGCGGACTCCCCGCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                        <  2:2362552/101‑1 (MQ=255)
                                                gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGCGGGTGAA.TGGC..g                                                        >  2:700788/1‑101 (MQ=255)
                                                 ctccccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                      <  2:8864254/98‑1 (MQ=255)
                                                 cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                      <  2:3030375/101‑1 (MQ=255)
                                                  tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                     <  2:2638148/101‑2 (MQ=255)
                                                   gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                    <  1:984402/101‑3 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                  <  1:772453/101‑5 (MQ=255)
                                                    cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                   >  2:8117116/1‑98 (MQ=255)
                                                     ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                 >  2:5877657/1‑96 (MQ=255)
                                                       cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccag                                                >  2:1972654/5‑95 (MQ=18)
                                                           tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                            <  1:3327279/101‑11 (MQ=255)
                                                                 aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCag                                       <  1:6927312/101‑2 (MQ=18)
                                                                    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcgc                                    >  2:7463652/1‑97 (MQ=18)
                                                                     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc                                 >  1:714694/1‑101 (MQ=18)
                                                                           ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccctg                           <  1:8368439/101‑4 (MQ=17)
                                                                             ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCACCGC.CCCCCTGcc                          >  1:4531221/1‑101 (MQ=17)
                                                                              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGccc                         >  2:4434598/1‑101 (MQ=18)
                                                                              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccccactgctc                        >  2:7215451/1‑94 (MQ=17)
                                                                               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGcccc                        <  2:3818141/101‑1 (MQ=17)
                                                                                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcg                       >  1:5174171/1‑101 (MQ=2)
                                                                                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcg                       >  2:4633388/1‑101 (MQ=18)
                                                                                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgc                      <  2:2808375/101‑2 (MQ=0)
                                                                                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggc                     >  2:4744540/1‑101 (MQ=17)
                                                                                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggc                     >  2:2661722/1‑101 (MQ=17)
                                                                                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcg                    >  2:7766288/1‑101 (MQ=18)
                                                                                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcg                    >  2:5374617/1‑101 (MQ=17)
                                                                                    atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccgg                   <  1:7555424/101‑1 (MQ=2)
                                                                                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCAGCCC.CCCCCTGCCCcggcgg                   >  2:5454249/1‑101 (MQ=18)
                                                                                     tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGt                  >  2:3144781/1‑101 (MQ=18)
                                                                                      ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGAGGC.AGCGCCCCCCTGCCCCGGCGGTc                 >  1:5013433/1‑101 (MQ=17)
                                                                                      ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcggcc                 >  1:8007750/1‑99 (MQ=17)
                                                                                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg               >  2:4482595/1‑101 (MQ=18)
                                                                                        gcagcaAGATGAAACCGGATGCCTTTCACCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg               <  1:6395668/101‑1 (MQ=17)
                                                                                          agcaAGATGAAACCGGATGGCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TCGC..GAAGCCAGAGGGGGGCAGCGG.CCCCCTGCCCCGGCGCTCCGTg             >  2:8193392/1‑101 (MQ=17)
                                                                                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg            <  1:1972714/101‑1 (MQ=17)
                                                                                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCAGCTC.CCCCCTGCCCCGGCGGTACGTGgccgcggc     >  2:1494372/1‑99 (MQ=14)
                                                                                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc     >  2:6677205/1‑101 (MQ=18)
                                                                                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt    <  2:1258087/101‑1 (MQ=17)
                                                                                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGCAGCCC.CCCCCTGCCCCGCCGGCCGGTGGGGGCGCCt    >  1:1127422/1‑101 (MQ=12)
                                                                                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt   >  1:4189766/1‑101 (MQ=18)
                                                                                                     |                                                                                                        
AGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.A.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTG  >  NC_014034/2493915‑2494113

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG