Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,023 C→A 3.4% intergenic (+71/‑171) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0230C→A 3.4% 18.6 ‑0.6 ‑0.2326intergenic (+71/‑171)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/5):  ref base (154/160):  total (161/165)
Fisher's exact test strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.63e-01

Only 100 of 343 total aligned reads displayed.

GCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493924‑2494121
                                                                                                     |                                                                                                         
  gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                         >  1:5995945/1‑101 (MQ=21)
  gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                         <  2:4257301/101‑1 (MQ=21)
  gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                         <  2:679440/101‑1 (MQ=21)
   ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                        <  2:92579/101‑1 (MQ=21)
   ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                        <  2:2465632/101‑1 (MQ=21)
   ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                        <  1:5678555/101‑1 (MQ=21)
    gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                       <  1:786711/101‑1 (MQ=21)
       cgcgGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                    <  1:2578386/101‑1 (MQ=21)
        gcgGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                                   <  2:1118283/101‑1 (MQ=21)
          ggCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                 <  1:6153934/101‑1 (MQ=21)
          ggCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                 <  2:5643495/101‑1 (MQ=21)
          ggCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                 <  1:4194395/101‑1 (MQ=21)
            cACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                               <  2:5947047/101‑1 (MQ=21)
            cACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                               >  2:7296832/1‑101 (MQ=21)
             aCGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                              >  1:314690/1‑101 (MQ=21)
             aCGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                              <  2:2770154/101‑1 (MQ=21)
               gACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                                                                                            >  2:7073967/1‑101 (MQ=21)
                aCAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                           >  2:6218555/1‑101 (MQ=21)
                aCAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                           <  1:3255690/101‑1 (MQ=21)
                aCAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                           <  2:379302/101‑1 (MQ=21)
                 cAGGCCGAGTAA.GGGGGGGTCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                          <  1:3587970/101‑1 (MQ=21)
                  aGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCCCAAGCGGACTCCCCCCGCGATATTTTCAGGAAGATGAAACCGGATGCCTTTCATATTGCCCGACat                                                                                         >  2:8955022/1‑101 (MQ=18)
                   ggCGGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                        <  1:6291246/101‑1 (MQ=18)
                   ggCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                        <  2:6865874/101‑1 (MQ=21)
                   ggCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                        >  2:7981973/1‑101 (MQ=21)
                      cGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                     <  2:812952/101‑1 (MQ=21)
                      cGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGCGATATTTGCAGCCAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                     >  1:1916425/1‑101 (MQ=18)
                       gAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                    <  1:7560390/101‑1 (MQ=18)
                          taagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                 <  1:1707389/97‑1 (MQ=255)
                          taagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                 >  1:836066/5‑101 (MQ=255)
                               gggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                            <  2:1946823/101‑1 (MQ=255)
                               gggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                            <  1:3931415/101‑1 (MQ=255)
                               gggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                            <  1:880170/101‑1 (MQ=255)
                                ggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                           <  2:6127768/101‑1 (MQ=255)
                                ggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                           <  1:1052772/101‑1 (MQ=255)
                                ggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGa                                                                           <  1:8155471/101‑1 (MQ=255)
                                  ggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                        <  2:7193470/101‑1 (MQ=255)
                                  ggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                        <  1:99830/101‑1 (MQ=255)
                                   gg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                       <  2:3627691/101‑1 (MQ=255)
                                      cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                     >  1:4154401/1‑101 (MQ=255)
                                          gCCCCCCGTCCGCAAGCGGAGTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGCGGGTGAA.TGGC..G.aag                                                              <  1:7251021/101‑3 (MQ=21)
                                          gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                              <  1:984402/101‑3 (MQ=255)
                                           cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                            <  1:8102985/101‑5 (MQ=255)
                                           cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                            <  1:9107171/101‑5 (MQ=255)
                                           cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                            >  1:6655669/1‑97 (MQ=255)
                                           cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                            <  1:772453/101‑5 (MQ=255)
                                              cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                          <  2:3582138/97‑7 (MQ=255)
                                               ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                         >  2:216859/4‑94 (MQ=255)
                                                  tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                      <  1:3327279/101‑11 (MQ=255)
                                                       aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCa                                                 <  1:3775851/101‑1 (MQ=18)
                                                        aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCag                                                <  1:6927312/101‑2 (MQ=18)
                                                           ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                             >  2:1313928/1‑97 (MQ=18)
                                                            gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGGcgcgcc                                             >  2:5435998/1‑96 (MQ=18)
                                                            gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c                                         >  1:714694/1‑101 (MQ=18)
                                                            gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c                                         >  2:3447109/1‑101 (MQ=18)
                                                            gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.AAGCG.c.c                                         >  2:264577/1‑101 (MQ=18)
                                                              cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.c.ccc                                       <  2:882595/101‑1 (MQ=17)
                                                              cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.c.ccc                                       <  1:7990667/101‑1 (MQ=17)
                                                                ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccc                                     >  2:5039857/1‑100 (MQ=18)
                                                                   cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccctgc                                  <  2:7704352/101‑5 (MQ=17)
                                                                   cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccctgc                                  <  1:560356/101‑5 (MQ=17)
                                                                     cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGccc                                 <  2:1626352/101‑1 (MQ=17)
                                                                     cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGccc                                 >  2:4432444/1‑101 (MQ=17)
                                                                      gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGcccc                                <  2:3767100/101‑1 (MQ=17)
                                                                      gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGcccc                                >  2:1083124/1‑101 (MQ=18)
                                                                       ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcg                               >  1:2146677/1‑101 (MQ=2)
                                                                       ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcg                               >  1:1831706/1‑101 (MQ=2)
                                                                       ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcg                               >  1:5174171/1‑101 (MQ=2)
                                                                          tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcggcg                            >  1:6499480/1‑101 (MQ=18)
                                                                           atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcgccgg                           <  1:8554535/101‑1 (MQ=2)
                                                                           atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcggcgg                           >  2:3274308/1‑101 (MQ=18)
                                                                            tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGt                          >  2:3144781/1‑101 (MQ=18)
                                                                             ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGAGGC.AGCGCC.CCCCTGCCCCGGCGGTc                         >  1:5013433/1‑101 (MQ=17)
                                                                              tGCAGCAAGATGAAACCGGATGCTTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTcc                        <  1:4666843/101‑1 (MQ=17)
                                                                              tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTcc                        <  2:6445306/101‑1 (MQ=17)
                                                                               gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCg                       >  2:4482595/1‑101 (MQ=18)
                                                                               gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCg                       >  2:664137/1‑101 (MQ=17)
                                                                               gcagcaAGATGAAACCGGATGCCTTTCACCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCg                       <  1:6395668/101‑1 (MQ=17)
                                                                                 agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTg                     <  2:3922043/101‑1 (MQ=17)
                                                                                   caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggc                   >  1:132532/1‑101 (MQ=18)
                                                                                   caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggc                   >  1:2412558/1‑101 (MQ=18)
                                                                                    aaGATGAAACCGGATGCCTTTCATGTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcg                  >  2:4385111/1‑101 (MQ=17)
                                                                                     aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcgg                 >  2:257776/1‑101 (MQ=18)
                                                                                        tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGAC.CCCCTGCCCCGGCGGTCCGCGGAGgcgc              >  1:8960017/1‑101 (MQ=17)
                                                                                         gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCTC.C.CCCCTGCCCCGGCGGTACGTGgccgcggc             >  2:1494372/1‑99 (MQ=14)
                                                                                         gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGcc             >  2:1991524/1‑101 (MQ=18)
                                                                                          aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCt            <  2:2828971/101‑1 (MQ=17)
                                                                                          aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGCAGCCC.C.CCCCTGCCCCGCCGGCCGGTGGGGGCGCCt            >  1:1127422/1‑101 (MQ=12)
                                                                                          aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCGGCCCCGGCGGGCCGTGGCGGCGCCt            >  1:1184577/1‑101 (MQ=14)
                                                                                          aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.CGCCCCTGCCCCGCCGGTCCGCGGGGGCCccc            >  1:290759/1‑100 (MQ=9)
                                                                                           aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt           >  2:766081/1‑101 (MQ=18)
                                                                                           aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt           <  2:5378781/101‑1 (MQ=17)
                                                                                             ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa         <  1:7402618/101‑1 (MQ=17)
                                                                                              cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        >  2:2872290/1‑101 (MQ=18)
                                                                                              cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGAC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        >  2:4739024/1‑101 (MQ=18)
                                                                                               ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg       >  2:5337349/1‑101 (MQ=18)
                                                                                                gATGCCTTTCATCTGACCCGAAATATCCCGGGGGGTGAA.CGGCA.A.AG.CCAGAGGGGGGCAACGC.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg      <  2:4675163/101‑1 (MQ=18)
                                                                                                 aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg     <  2:4910541/101‑1 (MQ=17)
                                                                                                  tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGGA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc    <  2:847206/101‑1 (MQ=17)
                                                                                                   gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg   >  2:7471263/1‑101 (MQ=18)
                                                                                                     |                                                                                                         
GCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493924‑2494121

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG