Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,048 G→A 4.3% intergenic (+96/‑146) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0480G→A 4.3% 18.1 ‑0.6 ‑0.2280intergenic (+96/‑146)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/6):  ref base (156/112):  total (162/118)
Fisher's exact test strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.74e-01

Only 100 of 322 total aligned reads displayed.

AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAA  >  NC_014034/2493948‑2494148
                                                                                                    |                                                                                                            
gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                              >  2:7827681/6‑101 (MQ=255)
gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCGTTCATCTTGCCCGAAATATCCCgg                                                                                                              >  2:5025270/6‑101 (MQ=18)
 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                             <  1:1707389/97‑1 (MQ=255)
 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                             >  2:7402294/5‑101 (MQ=255)
  tcttccgatccgcTGCCCCCGGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCcggg                                                                                                            <  1:7232375/91‑1 (MQ=21)
  aagggggggcCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCcggg                                                                                                            <  1:8801286/98‑1 (MQ=18)
    ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                                                          <  2:6346311/100‑1 (MQ=255)
     gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                                                         >  1:5378965/1‑100 (MQ=255)
      ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                        >  2:71198/1‑101 (MQ=255)
       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                       <  2:6127768/101‑1 (MQ=255)
       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                       <  1:1052772/101‑1 (MQ=255)
       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCATTTCATCTTGCCCGAAATATCCCGGGGGGCGa                                                                                                       >  1:121102/1‑101 (MQ=17)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                    <  2:8176758/101‑1 (MQ=255)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                    >  2:5189108/1‑101 (MQ=255)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                    <  1:99830/101‑1 (MQ=255)
           gcgcTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                                                                  <  2:1947132/101‑1 (MQ=255)
            cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                                 >  1:734792/1‑101 (MQ=255)
            cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                                 <  1:3525941/101‑1 (MQ=255)
            cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                                 <  2:5992274/101‑1 (MQ=255)
            cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGc                                                                                                 >  1:6159433/1‑101 (MQ=255)
              cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                            <  2:3030375/101‑1 (MQ=255)
               tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                           <  2:2638148/101‑2 (MQ=255)
                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                          >  1:4600842/1‑99 (MQ=255)
                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                          <  1:984402/101‑3 (MQ=255)
                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                          >  1:5765528/1‑99 (MQ=255)
                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                          <  1:6370809/101‑3 (MQ=255)
                 cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                        <  1:9107171/101‑5 (MQ=255)
                 cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                        >  1:6655669/1‑97 (MQ=255)
                     ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                     <  2:2574335/98‑8 (MQ=255)
                     ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                     >  2:3327175/4‑94 (MQ=255)
                      cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                    <  1:8117465/99‑9 (MQ=255)
                       gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                   <  2:1152574/100‑10 (MQ=255)
                        tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                                                  <  1:3327279/101‑11 (MQ=255)
                               gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCGTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCagg                                                                          >  1:5092912/1‑99 (MQ=18)
                                  gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc                                                                     >  2:3447109/1‑101 (MQ=18)
                                   aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc                                                                    >  2:8319079/1‑101 (MQ=18)
                                   aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc                                                                    >  1:489283/1‑101 (MQ=18)
                                    cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCG.cccc                                                                   <  2:882595/101‑1 (MQ=17)
                                    cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccc                                                                   >  1:2429977/1‑101 (MQ=18)
                                    cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccc                                                                   >  2:759813/1‑101 (MQ=18)
                                     tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCGG.AGGGGGGC.AGCG.cccc.c                                                                 >  2:1095838/1‑101 (MQ=18)
                                        ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccc.cctg                                                              <  1:8368439/101‑4 (MQ=17)
                                        ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccc.cccg                                                              >  1:6917471/1‑98 (MQ=17)
                                         cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cccc.cctgc                                                             <  2:7704352/101‑5 (MQ=17)
                                           cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGccc                                                            >  2:4432444/1‑101 (MQ=17)
                                           cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccccactgctc                                                            >  2:7215451/1‑94 (MQ=17)
                                            gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCCC.CTGcccc                                                           >  1:4013546/1‑101 (MQ=0)
                                            gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGcccc                                                           <  2:3818141/101‑1 (MQ=17)
                                            gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGcccc                                                           <  2:3767100/101‑1 (MQ=17)
                                            gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGcccc                                                           <  2:2158618/101‑1 (MQ=17)
                                             ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCcg                                                          >  2:4633388/1‑101 (MQ=18)
                                              gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCcgg                                                         >  2:8980044/1‑101 (MQ=18)
                                               atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCcggc                                                        >  2:2661722/1‑101 (MQ=17)
                                                 atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCCC.CTGCCCcgccgg                                                      <  1:7555424/101‑1 (MQ=2)
                                                 atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GACAGAGGGGGGC.AGCGCCCCC.CTGCCCcggcgg                                                      >  1:4170280/1‑101 (MQ=18)
                                                  tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGt                                                     >  2:3144781/1‑101 (MQ=18)
                                                   ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCcggcggcc                                                    >  1:8007750/1‑99 (MQ=17)
                                                    tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTcc                                                   <  2:6445306/101‑1 (MQ=17)
                                                     gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCg                                                  >  2:4482595/1‑101 (MQ=18)
                                                     gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCg                                                  >  2:1361329/1‑101 (MQ=18)
                                                       agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTg                                                <  2:3922043/101‑1 (MQ=17)
                                                         caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTggc                                              >  1:132532/1‑101 (MQ=18)
                                                          aaGATGAAACCGGATGCCTTTCATGTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTggcg                                             >  2:4385111/1‑101 (MQ=17)
                                                           aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTggcgg                                            >  2:3636282/1‑101 (MQ=18)
                                                              tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGACCCC.CTGCCCCGGCGGTCCGCGGAGgcgc                                         >  1:8960017/1‑101 (MQ=17)
                                                               gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGcc                                        <  2:5453497/101‑1 (MQ=17)
                                                               gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGcc                                        >  2:6677205/1‑101 (MQ=18)
                                                               gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGcc                                        >  2:6833472/1‑101 (MQ=18)
                                                                aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCt                                       <  2:2828971/101‑1 (MQ=17)
                                                                aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCt                                       <  2:1258087/101‑1 (MQ=17)
                                                                aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCCC.CCCC.CTGCCCCGCCGGCCGGTGGGGGCGCCt                                       >  1:1127422/1‑101 (MQ=12)
                                                                 aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCtt                                      <  2:5378781/101‑1 (MQ=17)
                                                                   ccGGATGCCTTTCATCTTGCCCGAAATGTCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                    <  1:382543/101‑1 (MQ=17)
                                                                               cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCCG.AGGGGGGC.AGCG.CCCCACTGCCCCGGCGGTCCCTGGCGGAGCCTTGACGGGCGGGGgcg                        >  1:1886174/1‑101 (MQ=18)
                                                                               cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                        >  1:7423214/1‑101 (MQ=18)
                                                                                 tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGggcg                      >  1:8243437/1‑101 (MQ=18)
                                                                                      cccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                 <  1:3049734/101‑1 (MQ=18)
                                                                                       ccGAAATATCCCGGGGGGTGCA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                <  1:5636856/101‑1 (MQ=18)
                                                                                       ccGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                <  2:397766/101‑1 (MQ=18)
                                                                                        cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa               <  1:7117265/101‑1 (MQ=18)
                                                                                        cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa               <  1:2693132/101‑1 (MQ=18)
                                                                                         gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa              <  2:9132726/101‑1 (MQ=18)
                                                                                          aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc             >  1:7796682/1‑101 (MQ=18)
                                                                                          aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc             <  1:8186217/101‑1 (MQ=18)
                                                                                           aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc            <  2:4839083/101‑1 (MQ=21)
                                                                                           aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc            >  2:3265307/1‑101 (MQ=21)
                                                                                           aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc            >  1:767916/1‑101 (MQ=21)
                                                                                            atatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg           >  2:8501128/1‑101 (MQ=21)
                                                                                            atatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg           <  2:4162885/101‑1 (MQ=21)
                                                                                             tatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGGGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa          >  1:4489356/1‑101 (MQ=18)
                                                                                              atCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGGGCCTTGACGGGCGGGGGCGCGGGCCTTAACCGaa         >  2:171146/1‑101 (MQ=18)
                                                                                              atCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa         >  1:2297369/1‑101 (MQ=21)
                                                                                              atCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa         >  2:2021604/1‑101 (MQ=21)
                                                                                               tCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa        >  2:1911123/1‑101 (MQ=21)
                                                                                                cccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt       >  2:4708802/1‑101 (MQ=21)
                                                                                                 ccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt      <  1:3316133/101‑1 (MQ=21)
                                                                                                 ccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt      <  2:5608592/101‑1 (MQ=21)
                                                                                                 ccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCCTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt      <  1:6462825/101‑1 (MQ=21)
                                                                                                   ggggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTga    >  2:4948536/1‑100 (MQ=25)
                                                                                                    gggggTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa   >  1:7724443/1‑101 (MQ=25)
                                                                                                    |                                                                                                            
AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAA  >  NC_014034/2493948‑2494148

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG