BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,494,061	G→A	4.4%	intergenic (+109/‑133)	RCAP_rcc02319 → / → RCAP_rcc02320	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,494,061	0	G→A	4.4%	20.5 ‑0.6 ‑0.3	271	intergenic (+109/‑133)	RCAP_rcc02319/RCAP_rcc02320	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (6/6): ref base (158/101): total (164/107)
Fisher's exact test strand distribution p-value = 5.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.70e-01

Only 100 of 290 total aligned reads displayed.


GCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493961‑2494152


gcTGCCCCCCGTCCGCAAGCGGACTCCCCGCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                                 <  2:2362552/101‑1 (MQ=255)


gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGCGGGTGAA.TGGC..g                                                                                                 >  2:700788/1‑101 (MQ=255)


 cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                               <  2:3030375/101‑1 (MQ=255)


  tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                              <  1:7350725/101‑2 (MQ=255)


   gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                             >  1:5765528/1‑99 (MQ=255)


   gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                             <  1:6370809/101‑3 (MQ=255)


   gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                             >  1:4600842/1‑99 (MQ=255)


    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                           >  1:6655669/1‑97 (MQ=255)


        ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                        <  2:2574335/98‑8 (MQ=255)


        ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                        >  2:3327175/4‑94 (MQ=255)


          gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                      <  2:1152574/100‑10 (MQ=255)


                aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCa                                                                               >  1:1258119/1‑101 (MQ=18)


                aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCa                                                                               <  1:3775851/101‑1 (MQ=18)


                    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCagcgc                                                                           >  2:7463652/1‑97 (MQ=18)


                     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAGAGGGGGGGcgcgcc                                                                           >  2:5435998/1‑96 (MQ=18)


                     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc                                                                        >  2:3447109/1‑101 (MQ=18)


                     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGG.AAGCG.cc                                                                        >  2:264577/1‑101 (MQ=18)


                     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc                                                                        >  2:4464824/1‑101 (MQ=17)


                      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.ccc                                                                       >  1:489283/1‑101 (MQ=18)


                       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCG.cccc                                                                      <  2:882595/101‑1 (MQ=17)


                       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCG.cccc                                                                      <  1:7990667/101‑1 (MQ=17)


                       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccc                                                                      >  1:2429977/1‑101 (MQ=18)


                       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccc                                                                      >  2:759813/1‑101 (MQ=18)


                          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccct                                                                   <  1:3132562/101‑3 (MQ=17)


                           ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccctg                                                                  <  1:8368439/101‑4 (MQ=17)


                           ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccccg                                                                  >  1:6917471/1‑98 (MQ=17)


                            cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccctgc                                                                 <  2:7704352/101‑5 (MQ=17)


                            cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccctgc                                                                 <  1:3318694/101‑5 (MQ=17)


                              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGccc                                                                <  2:1626352/101‑1 (MQ=17)


                              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.ccccactgctc                                                               >  2:7215451/1‑94 (MQ=17)


                               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGcccc                                                               <  2:3767100/101‑1 (MQ=17)


                               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGcccc                                                               <  2:2158618/101‑1 (MQ=17)


                               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGcccc                                                               <  2:3818141/101‑1 (MQ=17)


                               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGcccc                                                               >  2:1083124/1‑101 (MQ=18)


                                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcg                                                              >  1:1831706/1‑101 (MQ=2)


                                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcgg                                                             >  2:8980044/1‑101 (MQ=18)


                                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                            >  2:5005103/1‑99 (MQ=2)


                                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggc                                                            >  2:2661722/1‑101 (MQ=17)


                                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggc                                                            >  2:4744540/1‑101 (MQ=17)


                                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CAAGAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                          >  1:4507048/1‑101 (MQ=18)


                                      ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTc                                                        <  1:7429955/101‑1 (MQ=17)


                                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                                                       <  2:6445306/101‑1 (MQ=17)


                                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGCGGGGC.AGCGCCCCCCTGCCCCCGCGGTcc                                                       >  2:1769121/1‑101 (MQ=17)


                                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                                                      >  2:1361329/1‑101 (MQ=18)


                                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCg                                                      >  2:6821784/1‑101 (MQ=17)


                                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGt                                                     <  1:3359253/101‑1 (MQ=17)


                                          agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                    <  2:3922043/101‑1 (MQ=17)


                                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg                                                   <  1:1972714/101‑1 (MQ=17)


                                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggc                                                  <  1:7074252/101‑1 (MQ=17)


                                             aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TTGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                                 >  1:996582/1‑101 (MQ=18)


                                             aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                                 >  1:1405273/1‑101 (MQ=17)


                                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                >  2:3636282/1‑101 (MQ=18)


                                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                            >  2:6677205/1‑101 (MQ=18)


                                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                           <  2:1258087/101‑1 (MQ=17)


                                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                           <  2:2828971/101‑1 (MQ=17)


                                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGCAGCCC.CCCCCTGCCCCGCCGGCCGGTGGGGGCGCCt                                           >  1:1127422/1‑101 (MQ=12)


                                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCACCGC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  2:5557655/101‑1 (MQ=18)


                                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  2:5378781/101‑1 (MQ=17)


                                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          >  1:4189766/1‑101 (MQ=18)


                                                     acgggATGTCTATCATTGTGCCTGAAATAGCCGGGGTGGTGAA.TGGCG.G.A.G.CCAGAGTGAGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  2:44202/98‑1 (MQ=255)


                                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       >  1:5640879/1‑101 (MQ=18)


                                                        ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      >  2:5337349/1‑101 (MQ=18)


                                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CGAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                     >  1:7691488/1‑101 (MQ=18)


                                                         gATGCCTTTCATCTGACCCGAAATATCCCGGGGGGTGAA.CGGCA.A.A.G.CCAGAGGGGGGCAACGC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                     <  2:4675163/101‑1 (MQ=18)


                                                          aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    >  1:8056151/1‑101 (MQ=17)


                                                          aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    <  2:4910541/101‑1 (MQ=17)


                                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                   >  1:7193553/1‑101 (MQ=18)


                                                           cgCCTCTAATCTTGCCCGAAATATCCCGGGGGCTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtttgtcgag                                   <  2:602038/100‑8 (MQ=9)


                                                            gCCTTTCATCTTGCCCGAAATATCCCGGGGGATGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                                  >  2:4274964/1‑101 (MQ=18)


                                                              gttccagctggCCCGAAATAGCCCGGGGGGGGAG.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCGGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                <  1:2805703/91‑1 (MQ=12)


                                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               <  1:7765654/101‑1 (MQ=17)


                                                                    tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          >  1:5222058/1‑101 (MQ=18)


                                                                     cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                         >  1:5063574/1‑101 (MQ=18)


                                                                       tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                       >  1:6116736/1‑101 (MQ=18)


                                                                        gCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                      >  1:8714205/1‑101 (MQ=18)


                                                                         cccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                     <  1:9085299/101‑1 (MQ=18)


                                                                          ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  2:7916273/101‑1 (MQ=18)


                                                                           cGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                   <  1:1073244/101‑1 (MQ=18)


                                                                           cGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                   <  1:2693132/101‑1 (MQ=18)


                                                                            gAAATATCCGGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  2:4154282/101‑1 (MQ=18)


                                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  2:9132726/101‑1 (MQ=18)


                                                                              aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGGCTTGACGGGCGGGGGCGCGGGCATTAAcc                >  1:5422084/1‑101 (MQ=18)


                                                                              aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaaac                >  1:8235956/1‑99 (MQ=18)


                                                                              aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                >  2:3265307/1‑101 (MQ=21)


                                                                               atatCGCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCAGCAC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg               >  2:4530900/1‑101 (MQ=21)


                                                                               atatCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg               >  2:8501128/1‑101 (MQ=21)


                                                                                tatCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa              <  1:8179405/101‑1 (MQ=21)


                                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCGTTAACCGaa             >  1:3902521/1‑101 (MQ=21)


                                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  1:7636517/1‑101 (MQ=21)


                                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  2:2021604/1‑101 (MQ=21)


                                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  2:3126221/1‑101 (MQ=21)


                                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  1:2297369/1‑101 (MQ=21)


                                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  2:4010708/1‑101 (MQ=21)


                                                                                    ccGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCCTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          <  1:6462825/101‑1 (MQ=21)


                                                                                     cGGGGGGTGAA.GGGG..G.AGG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg         <  2:1916365/101‑1 (MQ=25)


                                                                                      ggggggCGAA.TGGC..GAA.G.CCAGAGGGGGGCACCGC.CCCCCTGCCCCGGCGGCCCGTGGGGGCGCCTTGACGGGAGGGGGCGCGGGCACTAACCGAAATTga        >  1:9198254/1‑100 (MQ=19)


                                                                                       gggggTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       >  1:7524311/1‑101 (MQ=25)


                                                                                       gggggTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       <  2:836053/101‑1 (MQ=25)


                                                                                         gggTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     >  2:8298949/1‑101 (MQ=25)


                                                                                          ggTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    <  2:5665382/101‑1 (MQ=25)


GCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493961‑2494152

Base quality scores:


ATCG

< 3 ≤


ATCG

< 16 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG