BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,494,067	A→G	93.8%	intergenic (+115/‑127)	RCAP_rcc02319 → / → RCAP_rcc02320	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,494,067	0	A→G	93.8%	27.2 ‑0.3 ‑0.0	244	intergenic (+115/‑127)	RCAP_rcc02319/RCAP_rcc02320	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (136/81): ref base (8/6): total (154/90)
Fisher's exact test strand distribution p-value = 7.78e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.80e-01

Only 100 of 260 total aligned reads displayed.


AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGG.GGGCAAGCG.C.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493977‑2494152


aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGCa                                                                                 >  1:1258119/1‑101 (MQ=18)


    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGCagcgc                                                                             >  1:3768029/1‑97 (MQ=17)


    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGCagcgc                                                                             >  2:1313928/1‑97 (MQ=18)


     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAGAGGGG.GGGcgcgcc                                                                             >  2:5435998/1‑96 (MQ=18)


     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCG.c.c                                                                         >  1:714694/1‑101 (MQ=18)


       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG.AGGG.GGGC.AGCG.c.ccc                                                                       <  1:7990667/101‑1 (MQ=17)


       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG.AGGG.GGGC.AGCG.c.ccc                                                                       <  2:882595/101‑1 (MQ=17)


          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCG.c.ccc.cct                                                                   <  1:1741180/101‑3 (MQ=17)


          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCG.c.ccc.cct                                                                   <  1:3132562/101‑3 (MQ=17)


             ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGCACCGC.C.CCC.CTGcc                                                                 >  1:4531221/1‑101 (MQ=17)


              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGccc                                                                <  2:1626352/101‑1 (MQ=17)


              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGccc                                                                >  2:4434598/1‑101 (MQ=18)


               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGcccc                                                               >  2:1083124/1‑101 (MQ=18)


               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGcccc                                                               <  2:2158618/101‑1 (MQ=17)


                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAG.GGGGCAGCG.C.CCC.CTGCCCcg                                                              >  1:2146677/1‑101 (MQ=2)


                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAG.GGGGCAGCG.C.CCC.CTGCCCcg                                                              >  1:5174171/1‑101 (MQ=2)


                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCcgg                                                             >  2:8980044/1‑101 (MQ=18)


                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAG.GGGGCAGCG.C.CCC.CTGCCCcgcc                                                            >  2:5005103/1‑99 (MQ=2)


                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCcggc                                                            >  2:2661722/1‑101 (MQ=17)


                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCcggcg                                                           >  2:7766288/1‑101 (MQ=18)


                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCcggcg                                                           >  2:4504676/1‑101 (MQ=18)


                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCcggcg                                                           >  2:5374617/1‑101 (MQ=17)


                    atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAG.GGGGCAGCG.C.CCC.CTGCCCcgccgg                                                          <  1:8554535/101‑1 (MQ=2)


                    atTTGCGGCAAGATGAAGGGGCATG.ATTTCACCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAG.GGGGCAGCG.C.CCC.CTGCCCcgccgg                                                          <  1:6583533/101‑1 (MQ=2)


                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGCAGCCC.C.CCC.CTGCCCcggcgg                                                          >  2:5454249/1‑101 (MQ=18)


                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCcggcgg                                                          >  1:9097314/1‑101 (MQ=17)


                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCcggcgg                                                          >  2:3274308/1‑101 (MQ=18)


                     tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGt                                                         >  2:3144781/1‑101 (MQ=18)


                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCg                                                      >  2:664137/1‑101 (MQ=17)


                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCg                                                      <  1:700798/101‑1 (MQ=17)


                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCg                                                      >  2:1361329/1‑101 (MQ=18)


                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGt                                                     <  1:3359253/101‑1 (MQ=17)


                          agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTg                                                    <  2:3922043/101‑1 (MQ=17)


                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTggc                                                  >  1:2412558/1‑101 (MQ=18)


                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTggc                                                  >  1:132532/1‑101 (MQ=18)


                             aataTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTggcg                                                 >  1:2602822/4‑101 (MQ=17)


                             aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TTGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTggcg                                                 >  1:996582/1‑101 (MQ=18)


                               gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTggcggc                                               >  2:7092140/1‑101 (MQ=18)


                               gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGACCCAGCGGTCCGTggcggc                                               >  2:600238/1‑101 (MQ=17)


                                 tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGAC.CCC.CTGCCCCGGCGGTCCGCGGAGgcgc                                             >  1:8960017/1‑101 (MQ=17)


                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGcc                                            >  2:6833472/1‑101 (MQ=18)


                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCt                                           <  2:2828971/101‑1 (MQ=17)


                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCt                                           >  2:3606184/1‑101 (MQ=18)


                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGG.GGGCAGCCC.C.CCC.CTGCCCCGCCGGCCGGTGGGGGCGCCt                                           >  1:1127422/1‑101 (MQ=12)


                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCG.CGCCC.CTGCCCCGCCGGTCCGCGGGGGCCccc                                           >  1:290759/1‑100 (MQ=9)


                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  2:4600707/101‑1 (MQ=17)


                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          >  1:4189766/1‑101 (MQ=18)


                                     acgggATGTCTATCATTGTGCCTGAAATAGCCGGGGTGGTGAA.TGGCG.G.A.G.CCAGAGTG.AGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  2:44202/98‑1 (MQ=255)


                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  2:3848032/101‑1 (MQ=17)


                                      ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                        <  1:7402618/101‑1 (MQ=17)


                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  2:3641976/101‑1 (MQ=17)


                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CGAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                     >  1:7691488/1‑101 (MQ=18)


                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGCCGGGGGGGGCGCCTGGAcgg                                     >  1:5046047/1‑101 (MQ=9)


                                          aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    <  2:4910541/101‑1 (MQ=17)


                                            gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                                  >  2:7471263/1‑101 (MQ=18)


                                            gCCTTTCATCTTGCCCGAAATATCCCGGGGGATGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                                  >  2:4274964/1‑101 (MQ=18)


                                             ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                 >  1:6425591/1‑101 (MQ=18)


                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               <  1:7765654/101‑1 (MQ=17)


                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               >  1:4690823/1‑101 (MQ=17)


                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGGGGCGCCTTGACCGGCgggggg                             >  2:512096/1‑100 (MQ=17)


                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             <  1:8050039/101‑1 (MQ=18)


                                                  cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCCGAGGG.GGGC.AGCG.C.CCCACTGCCCCGGCGGTCCCTGGCGGAGCCTTGACGGGCGGGGgcg                            >  1:1886174/1‑101 (MQ=18)


                                                  cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGCAGCGG.C.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                            >  2:8318466/1‑101 (MQ=18)


                                                    tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGggcg                          >  1:8243437/1‑101 (MQ=18)


                                                      ttGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                        >  2:1775279/1‑101 (MQ=18)


                                                      ttGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                        <  2:3767920/101‑1 (MQ=18)


                                                       tGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCCTGACGGGCGGGGGGCCGGGc                       >  2:2903674/1‑101 (MQ=12)


                                                          ccGAAATATCCCGGGGGGTGCA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  1:5636856/101‑1 (MQ=18)


                                                          ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  2:1145603/101‑1 (MQ=18)


                                                          ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  2:7916273/101‑1 (MQ=18)


                                                           cGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                   <  1:1073244/101‑1 (MQ=18)


                                                            gaaagttCCCGGGGGGTGAT.TGGC..G.A.G.TCCAGGGGTGGGCAGGGC.T.CCC.CTGCCCCGGCGGTCCGTGGCGGCTCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  2:3392940/95‑1 (MQ=255)


                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  2:9132726/101‑1 (MQ=18)


                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  >  2:6707653/1‑101 (MQ=18)


                                                             aaaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 <  1:8186217/101‑1 (MQ=18)


                                                              aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                <  2:4839083/101‑1 (MQ=21)


                                                              aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                <  2:2070887/101‑1 (MQ=21)


                                                              aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                >  2:7392681/1‑101 (MQ=21)


                                                               atatCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg               >  2:8501128/1‑101 (MQ=21)


                                                                tatCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGGGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa              >  1:4489356/1‑101 (MQ=18)


                                                                tatCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa              >  2:2383626/1‑101 (MQ=21)


                                                                tatCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa              >  2:8370627/1‑101 (MQ=21)


                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCGTTAACCGaa             >  1:3902521/1‑101 (MQ=21)


                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  1:7636517/1‑101 (MQ=21)


                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  2:5643118/1‑101 (MQ=21)


                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  2:4010708/1‑101 (MQ=21)


                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             <  1:6218794/101‑1 (MQ=21)


                                                                  tCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa            <  1:5877892/101‑1 (MQ=21)


                                                                   cccGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt           >  2:4708802/1‑101 (MQ=21)


                                                                    ccGGGGGGTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          <  2:4151863/101‑1 (MQ=21)


                                                                     cGGGGGGTGAA.GGGG..G.AGG.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg         <  2:1916365/101‑1 (MQ=25)


                                                                      ggggggTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc        >  2:6399056/1‑101 (MQ=25)


                                                                       gggggTGAA.TGGC..GAA.GCCAG.AGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       >  1:689785/1‑101 (MQ=25)


                                                                       gggggTGAA.TGGC..GAA.GCCAG.AGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       >  1:7524311/1‑101 (MQ=25)


                                                                       gggggTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       <  1:7452579/101‑1 (MQ=25)


                                                                         gggTGAA.TGGC..GAA.GCCAG.AGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     >  2:2647800/1‑101 (MQ=25)


                                                                         gggTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  2:7627618/101‑1 (MQ=25)


                                                                          ggTGAA.TGGC..GAA.GCCAG.AGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    >  1:5404995/1‑101 (MQ=25)


                                                                          ggTGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    <  1:2708145/101‑1 (MQ=25)


                                                                            tGAA.TGGC..GAA.G.CCAGAGGG.GGGC.AGCGCC.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa  >  1:570159/1‑101 (MQ=25)


AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGG.GGGCAAGCG.C.CCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493977‑2494152

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG