BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,494,070	G→A	4.1%	intergenic (+118/‑124)	RCAP_rcc02319 → / → RCAP_rcc02320	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,494,070	0	G→A	4.1%	15.7 ‑1.0 ‑0.0	291	intergenic (+118/‑124)	RCAP_rcc02319/RCAP_rcc02320	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (6/6): ref base (185/94): total (191/100)
Fisher's exact test strand distribution p-value = 3.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 313 total aligned reads displayed.


AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170


aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCa                                                                                                 >  2:2139550/1‑101 (MQ=18)


 aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCag                                                                                                <  2:9181179/101‑2 (MQ=18)


    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCagcgc                                                                                             >  2:7463652/1‑97 (MQ=18)


    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCagcgc                                                                                             >  1:3768029/1‑97 (MQ=17)


     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAGAGGGGGGGcgcgcc                                                                                             >  2:5435998/1‑96 (MQ=18)


     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc                                                                                          >  1:714694/1‑101 (MQ=18)


     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc                                                                                          >  2:7126503/1‑101 (MQ=18)


     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGG.AAGCG.cc                                                                                          >  2:264577/1‑101 (MQ=18)


     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc                                                                                          >  2:4464824/1‑101 (MQ=17)


       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCG.cccc                                                                                        <  1:7990667/101‑1 (MQ=17)


       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccc                                                                                        >  2:759813/1‑101 (MQ=18)


          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccct                                                                                     <  1:3132562/101‑3 (MQ=17)


           ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccctg                                                                                    <  1:8368439/101‑4 (MQ=17)


           ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccccg                                                                                    >  1:6917471/1‑98 (MQ=17)


            cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccctgc                                                                                   <  2:7704352/101‑5 (MQ=17)


            cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccctgc                                                                                   <  1:560356/101‑5 (MQ=17)


               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGcccc                                                                                 <  2:3818141/101‑1 (MQ=17)


               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGcccc                                                                                 <  2:3767100/101‑1 (MQ=17)


               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGcccc                                                                                 >  2:1083124/1‑101 (MQ=18)


                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcg                                                                                >  1:1831706/1‑101 (MQ=2)


                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcg                                                                                >  1:5174171/1‑101 (MQ=2)


                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcgg                                                                               <  2:314687/101‑1 (MQ=17)


                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                                              >  2:5005103/1‑99 (MQ=2)


                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggc                                                                              >  2:4744540/1‑101 (MQ=17)


                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggc                                                                              >  2:2661722/1‑101 (MQ=17)


                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcg                                                                             >  2:4504676/1‑101 (MQ=18)


                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcg                                                                             >  1:6499480/1‑101 (MQ=18)


                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcg                                                                             >  2:5374617/1‑101 (MQ=17)


                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                                            >  2:3274308/1‑101 (MQ=18)


                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.CGCGCCCCCCTGCCCcggcggccg                                                                         >  2:3906303/1‑98 (MQ=17)


                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                                                                         <  2:6445306/101‑1 (MQ=17)


                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGCGGGGC.AGCGCCCCCCTGCCCCCGCGGTcc                                                                         >  2:1769121/1‑101 (MQ=17)


                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                                                                        >  2:1361329/1‑101 (MQ=18)


                        gcagcaAGATGAAACCGGATGCCTTTCACCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                                                                        <  1:6395668/101‑1 (MQ=17)


                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGt                                                                       <  1:3359253/101‑1 (MQ=17)


                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGt                                                                       >  2:853078/1‑101 (MQ=17)


                          agcaAGATGAAACCGGATGGCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TCGC..GAA.G.CCAGAGGGGGGCAGCGG.CCCCCTGCCCCGGCGCTCCGTg                                                                      >  2:8193392/1‑101 (MQ=17)


                          agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                                      >  1:142136/1‑101 (MQ=17)


                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg                                                                     <  1:1972714/101‑1 (MQ=17)


                             aataTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                                                   >  1:2602822/4‑101 (MQ=17)


                             aaGATGAAACCGGATGCCTTTCATGTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                                                   >  2:4385111/1‑101 (MQ=17)


                             aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TTGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                                                   >  1:996582/1‑101 (MQ=18)


                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                                  >  2:3636282/1‑101 (MQ=18)


                                 tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGACCCCCTGCCCCGGCGGTCCGCGGAGgcgc                                                               >  1:8960017/1‑101 (MQ=17)


                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                                              >  2:6833472/1‑101 (MQ=18)


                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                                              >  2:1991524/1‑101 (MQ=18)


                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                                             <  2:2828971/101‑1 (MQ=17)


                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCACCGC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                                            <  2:5557655/101‑1 (MQ=18)


                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                                            <  2:4600707/101‑1 (MQ=17)


                                      ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                                          <  2:7773691/101‑1 (MQ=17)


                                      ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                                          <  1:7402618/101‑1 (MQ=17)


                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CGAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                                       >  1:7691488/1‑101 (MQ=18)


                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGCCGGGGGGGGCGCCTGGAcgg                                                       >  1:5046047/1‑101 (MQ=9)


                                          aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                                      <  2:4910541/101‑1 (MQ=17)


                                          aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                                      >  1:8056151/1‑101 (MQ=17)


                                              gttccagctggCCCGAAATAGCCCGGGGGGGGAG.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCGGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                                  <  1:2805703/91‑1 (MQ=12)


                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 <  1:7765654/101‑1 (MQ=17)


                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 >  1:4690823/1‑101 (MQ=17)


                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGGGGCGCCTTGACCGGCgggggg                                               >  2:512096/1‑100 (MQ=17)


                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                                               <  2:3424366/101‑1 (MQ=18)


                                                  cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCAGCGG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                                              >  2:8318466/1‑101 (MQ=18)


                                                      tggCCCGAAATTCCCGGGGGGGTGAT.TGGC..G.ACG.CCGGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                                          <  1:4387371/99‑1 (MQ=21)


                                                       tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                                         >  1:6116736/1‑101 (MQ=18)


                                                       tGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCCTGACGGGCGGGGGGCCGGGc                                         >  2:2903674/1‑101 (MQ=12)


                                                         cccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                                       >  2:8994577/1‑101 (MQ=18)


                                                          ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                      <  1:1313960/101‑1 (MQ=18)


                                                           cGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                     <  1:1073244/101‑1 (MQ=18)


                                                           cGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                     <  1:2693132/101‑1 (MQ=18)


                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                                    >  2:6707653/1‑101 (MQ=18)


                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                                    >  2:6216261/1‑101 (MQ=18)


                                                              aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaaac                                  >  1:8235956/1‑99 (MQ=18)


                                                              aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                                  >  2:1240412/1‑101 (MQ=21)


                                                               atatCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg                                 <  2:4162885/101‑1 (MQ=21)


                                                                tatCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                >  2:8370627/1‑101 (MQ=21)


                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCGTTAACCGaa                               >  1:3902521/1‑101 (MQ=21)


                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                               >  1:866606/1‑101 (MQ=21)


                                                                       gggggTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         <  1:7452579/101‑1 (MQ=25)


                                                                         gggTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                       >  1:4525976/1‑101 (MQ=25)


                                                                         gggTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                       >  1:7547530/1‑101 (MQ=25)


                                                                          ggTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                      >  1:5404995/1‑101 (MQ=25)


                                                                          ggTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                      >  1:1176138/1‑101 (MQ=25)


                                                                           gTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     >  2:4095384/1‑101 (MQ=25)


                                                                            tGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa                    >  1:570159/1‑101 (MQ=25)


                                                                                         gaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  2:5949280/9‑101 (MQ=38)


                                                                                         gaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  2:469953/9‑101 (MQ=38)


                                                                                         gaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  2:1583342/9‑101 (MQ=38)


                                                                                          aagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           >  2:563708/8‑101 (MQ=38)


                                                                                          aagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           >  2:7377002/8‑101 (MQ=38)


                                                                                            gccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATcc         >  1:912172/6‑101 (MQ=38)


                                                                                            gccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATcc         >  2:2236934/6‑101 (MQ=38)


                                                                                             ccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        >  2:1909842/5‑101 (MQ=38)


                                                                                             ccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        >  1:1514445/5‑101 (MQ=38)


                                                                                              cagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       <  1:216862/98‑1 (MQ=38)


                                                                                               agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTcc      >  1:2343963/3‑100 (MQ=38)


                                                                                               agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTcc      >  2:3958558/3‑100 (MQ=38)


                                                                                                 aGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc    >  1:6644998/1‑101 (MQ=255)


                                                                                                 aGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc    >  1:7058291/1‑101 (MQ=255)


                                                                                                 aGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc    >  2:3113678/1‑101 (MQ=255)


                                                                                                 aGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc    >  1:764454/1‑101 (MQ=255)


                                                                                                 aGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc    >  2:8894962/1‑101 (MQ=255)


AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170

Base quality scores:


ATCG

< 3 ≤


ATCG

< 16 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG