Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,523,952 T→G 100% I573S (ATC→AGC)  ppiD → peptidyl‑prolyl cis‑trans isomerase D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,523,9520T→G100.0% 1307.3 344I573S (ATC→AGC) ppiDpeptidyl‑prolyl cis‑trans isomerase D
Reads supporting (aligned to +/- strand):  new base (192/152):  ref base (0/0):  total (192/152)

Only 100 of 352 total aligned reads displayed.

CGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGATCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCAA  >  NC_014034/2523854‑2524049
                                                                                                  |                                                                                                 
 gACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCa                                                                                                <  2:1706919/101‑1 (MQ=255)
  aCAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAg                                                                                               >  1:6133335/1‑101 (MQ=255)
  aCAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAg                                                                                               >  1:6017422/1‑101 (MQ=255)
   cAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGa                                                                                              >  1:5047385/1‑101 (MQ=255)
   cAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGa                                                                                              <  1:8230570/101‑1 (MQ=255)
    aaGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             >  1:4439329/1‑101 (MQ=255)
    aaGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             <  2:2571374/101‑1 (MQ=255)
    aaGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             >  2:1735528/1‑101 (MQ=255)
    aaGGTCTGTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             <  1:2201067/101‑1 (MQ=255)
      ggTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGc                                                                                           <  1:3367956/101‑1 (MQ=255)
      ggTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGc                                                                                           <  1:3666078/101‑1 (MQ=255)
        tCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTc                                                                                         <  1:5827858/101‑1 (MQ=255)
        tCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTc                                                                                         <  2:3166118/101‑1 (MQ=255)
        tCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTc                                                                                         <  1:1229695/101‑1 (MQ=255)
         cTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCg                                                                                        <  1:9174446/101‑1 (MQ=255)
         cTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCg                                                                                        >  1:7916629/1‑101 (MQ=255)
         cTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCg                                                                                        <  2:6375843/101‑1 (MQ=255)
          tttGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCgg                                                                                       <  1:7841521/101‑1 (MQ=255)
            tGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgcg                                                                                     >  1:1003471/1‑101 (MQ=255)
              tcgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGcc                                                                                   <  2:8704982/101‑1 (MQ=255)
                gtcACCGTGCGCGCGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGa                                                                                 <  2:8433231/101‑1 (MQ=255)
                gccACGGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGa                                                                                 <  2:3410111/99‑1 (MQ=255)
                  cACCGTGCGCGCGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATc                                                                               <  2:8231591/101‑1 (MQ=255)
                  cACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATc                                                                               >  2:6724677/1‑101 (MQ=255)
                    ccttGCGCGGGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTg                                                                             <  2:6649172/98‑1 (MQ=255)
                    ccGTGCGCGCGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTg                                                                             <  2:2688674/101‑1 (MQ=255)
                    ccGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTg                                                                             <  1:173549/101‑1 (MQ=255)
                         cgcgcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTggcggc                                                                        <  1:5549935/101‑1 (MQ=255)
                          gcgggGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGcc                                                                       <  1:685551/101‑1 (MQ=255)
                            gcgGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCtt                                                                     <  2:4625497/101‑1 (MQ=255)
                            gcgGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCtt                                                                     <  1:7979583/101‑1 (MQ=255)
                               gTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTa                                                                  >  2:6994120/1‑101 (MQ=255)
                                 gAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTAcc                                                                >  2:4185988/1‑100 (MQ=255)
                                 gAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACg                                                                >  2:221304/1‑101 (MQ=255)
                                  aaaCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGc                                                               >  1:4065157/1‑101 (MQ=255)
                                   acccggccgatccggccgatCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGcc                                                              <  1:2557394/99‑1 (MQ=255)
                                    aCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGccc                                                             <  1:1460590/101‑1 (MQ=255)
                                      cggccgatccggccgatcCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGt                                                           <  2:3645486/101‑1 (MQ=255)
                                       ggccgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTg                                                          >  1:6234523/1‑101 (MQ=255)
                                        gccgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGc                                                         <  1:2663567/101‑1 (MQ=255)
                                         ccgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGcc                                                        <  2:448512/101‑1 (MQ=255)
                                         ccgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGcc                                                        <  2:8985131/101‑1 (MQ=255)
                                         ccgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGcc                                                        <  1:4351959/101‑1 (MQ=255)
                                         ccgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGcc                                                        <  1:6765868/101‑1 (MQ=255)
                                          cgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcg                                                       >  1:7706791/1‑101 (MQ=255)
                                            atccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcgcg                                                     >  1:353769/1‑101 (MQ=255)
                                             tccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCgcgc                                                    <  2:8153035/101‑1 (MQ=255)
                                                 gccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggc                                                >  2:789189/1‑101 (MQ=255)
                                                  ccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggcc                                               >  1:7037279/1‑101 (MQ=255)
                                                  ccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggcc                                               >  1:2102020/1‑101 (MQ=255)
                                                    gatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccga                                             >  1:8155805/1‑101 (MQ=255)
                                                    gatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccga                                             >  1:562501/1‑101 (MQ=255)
                                                    gatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccga                                             >  1:3257166/1‑101 (MQ=255)
                                                      tccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgagg                                           <  1:8491695/101‑1 (MQ=255)
                                                       cccGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggc                                          >  2:2017007/1‑101 (MQ=255)
                                                        ccGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggcc                                         <  1:9013198/101‑1 (MQ=255)
                                                        ccGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggcc                                         >  1:2420947/1‑101 (MQ=255)
                                                         cGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggccg                                        >  1:6535706/1‑101 (MQ=255)
                                                         cGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggccg                                        >  2:3962829/1‑101 (MQ=255)
                                                             cttgTGCAGCTGCGCAAGACCTTTGACCCCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGGGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGAt                                    <  1:4254350/98‑1 (MQ=255)
                                                             cTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCGGAGGCCggggc                                    >  2:1338636/1‑99 (MQ=255)
                                                             cTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGAt                                    >  2:7059719/1‑101 (MQ=255)
                                                             cTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGAt                                    >  2:4935352/1‑101 (MQ=255)
                                                              tGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATc                                   >  1:5691006/1‑101 (MQ=255)
                                                              tGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATc                                   >  2:583048/1‑101 (MQ=255)
                                                              tGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATc                                   <  2:3772366/101‑1 (MQ=255)
                                                               ggTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCTGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCa                                  >  1:184035/1‑101 (MQ=255)
                                                               ggTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCa                                  >  2:6880569/1‑101 (MQ=255)
                                                               ggTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCa                                  >  1:593930/1‑101 (MQ=255)
                                                                gTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAc                                 >  2:8051216/1‑101 (MQ=255)
                                                                 tGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAcc                                <  2:1205761/101‑1 (MQ=255)
                                                                  gCAGCTGCGCAAGACCTTTGACCCCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAccc                               <  2:8554261/101‑1 (MQ=255)
                                                                   cAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCt                              >  2:7253798/1‑101 (MQ=255)
                                                                    aGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTc                             >  1:3783244/1‑101 (MQ=255)
                                                                     gCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCg                            >  1:2720127/1‑101 (MQ=255)
                                                                      cTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGa                           >  1:6423086/1‑101 (MQ=255)
                                                                        gcgcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACa                         <  1:7670266/101‑1 (MQ=255)
                                                                            aaGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgcgc                     >  2:5557336/1‑101 (MQ=255)
                                                                            aaGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgcgc                     >  2:5292703/1‑101 (MQ=255)
                                                                            aaGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgcgc                     >  1:87928/1‑101 (MQ=255)
                                                                            aaGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgcgc                     <  1:7432212/101‑1 (MQ=255)
                                                                             aGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGcgcg                    >  1:4873640/1‑101 (MQ=255)
                                                                             aGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGcgcg                    >  2:1762208/1‑101 (MQ=255)
                                                                              gACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCga                   <  2:6283959/101‑1 (MQ=255)
                                                                               aCCTTTGACCCCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgat                  <  1:3197142/101‑1 (MQ=255)
                                                                               aCCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgat                  <  2:7614443/101‑1 (MQ=255)
                                                                                ccTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatg                 <  1:5145004/101‑1 (MQ=255)
                                                                                 cTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatga                >  1:6656584/1‑101 (MQ=255)
                                                                                 cTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatga                <  2:3257066/101‑1 (MQ=255)
                                                                                 cTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatga                >  2:5727081/1‑101 (MQ=255)
                                                                                  tttGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatgat               >  1:548951/1‑101 (MQ=255)
                                                                                   ttGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATc              >  1:5351664/1‑101 (MQ=255)
                                                                                   ttGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATc              <  2:3522162/101‑1 (MQ=255)
                                                                                   ttGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATc              >  2:6082325/1‑101 (MQ=255)
                                                                                    tGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCg             >  2:6151721/1‑101 (MQ=255)
                                                                                      acacCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCt           >  1:8392648/1‑101 (MQ=255)
                                                                                      acacCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGGGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGCTGATCGCt           >  1:1409340/1‑101 (MQ=255)
                                                                                            ggCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTg     <  1:7318424/101‑1 (MQ=255)
                                                                                             gCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGGGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACGGCGCGATGCTCGCTCCCGGGc    >  2:3062401/1‑101 (MQ=255)
                                                                                               tGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGGGCCGCGCGGGGCGAGGCCGGGAGCACACGCCAAAGAGCGATGATCGCTGGCGTGCac  >  1:8948556/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
CGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGATCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCAA  >  NC_014034/2523854‑2524049

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG