Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,531,575 G→A 100% L542L (CTC→CTT RCAP_rcc02362 ← competence protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,531,5750G→A100.0% 1249.9 318L542L (CTC→CTTRCAP_rcc02362competence protein
Reads supporting (aligned to +/- strand):  new base (163/155):  ref base (0/0):  total (163/155)

Only 100 of 357 total aligned reads displayed.

AGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACGAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCG  >  NC_014034/2531477‑2531672
                                                                                                  |                                                                                                 
aGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaag                                                                                                 <  2:654458/101‑1 (MQ=255)
aGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaag                                                                                                 <  1:1763613/101‑1 (MQ=255)
aGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCACACCGCAAAGGCCAcaag                                                                                                 >  2:1418261/1‑101 (MQ=255)
    cccgcccCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaagcgcg                                                                                             <  2:799802/101‑1 (MQ=255)
           cTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCa                                                                                      <  1:1261598/101‑1 (MQ=255)
           cTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCa                                                                                      <  1:2908454/101‑1 (MQ=255)
            tCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAg                                                                                     >  1:1951045/1‑101 (MQ=255)
             cGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGGAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGCGCGCCAGc                                                                                    >  1:2634145/1‑101 (MQ=255)
               ggCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCAt                                                                                  <  2:576068/101‑1 (MQ=255)
               ggCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCAt                                                                                  <  2:4580815/101‑1 (MQ=255)
               ggCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCAt                                                                                  <  1:8398417/101‑1 (MQ=255)
                ggatcatcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATc                                                                                 <  1:5005108/98‑1 (MQ=255)
                gCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATc                                                                                 <  1:3823623/101‑1 (MQ=255)
                 cGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcg                                                                                >  2:7513156/1‑101 (MQ=255)
                 cGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcg                                                                                <  1:7703199/101‑1 (MQ=255)
                  gTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAATCGCGAGCGCCAGCATcgc                                                                               >  1:5730462/1‑101 (MQ=255)
                   tcatcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgcc                                                                              <  1:3940533/101‑1 (MQ=255)
                   tcatcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgcc                                                                              <  2:2377337/101‑1 (MQ=255)
                    catcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgccg                                                                             >  2:8223504/1‑101 (MQ=255)
                    catcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgccg                                                                             >  2:316379/1‑101 (MQ=255)
                    caccaccccGACAAGCGCGCCCGCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgccg                                                                             <  2:4236937/95‑1 (MQ=255)
                     atcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgccgc                                                                            <  1:1823932/101‑1 (MQ=255)
                          cccGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGa                                                                       <  1:3224266/101‑1 (MQ=255)
                           ccGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGaa                                                                      >  1:2252815/1‑101 (MQ=255)
                           ccGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCCGaa                                                                      <  1:7204712/101‑1 (MQ=255)
                               cAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAcgcg                                                                  >  1:8442640/1‑101 (MQ=255)
                                aaGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACgcgc                                                                 >  2:4682196/1‑101 (MQ=255)
                                 aGCGCGCCCCCGGGCGCGAGCAGCAGCGCCGGACGGGGGGGAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGcc                                                                <  1:7844933/101‑1 (MQ=255)
                                  gcgcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGccc                                                               <  1:4584912/101‑1 (MQ=255)
                                   cgcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGcccc                                                              <  1:4876322/101‑1 (MQ=255)
                                    gcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcg                                                             <  2:2433245/101‑1 (MQ=255)
                                     cgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcgc                                                            <  1:7318768/101‑1 (MQ=255)
                                      gcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcgcg                                                           <  2:5116742/101‑1 (MQ=255)
                                      gcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcgcg                                                           <  1:800575/101‑1 (MQ=255)
                                      gcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcgcg                                                           >  1:1588252/1‑101 (MQ=255)
                                      gcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcgcg                                                           >  2:1901672/1‑101 (MQ=255)
                                      gcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcgcg                                                           >  2:292016/1‑101 (MQ=255)
                                        ccccGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGAc                                                         <  1:3451831/101‑1 (MQ=255)
                                        ccccGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGAc                                                         <  2:8473864/101‑1 (MQ=255)
                                        ccccGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGAc                                                         >  1:5555982/1‑101 (MQ=255)
                                           cGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGc                                                      <  2:4861217/101‑1 (MQ=255)
                                           cGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGc                                                      >  1:5473640/1‑101 (MQ=255)
                                           cGGGCCCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGc                                                      <  2:1037264/101‑1 (MQ=255)
                                            gggCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCa                                                     >  1:7563508/1‑101 (MQ=255)
                                              gcgcGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAcc                                                   <  2:264080/101‑1 (MQ=255)
                                              gcgcGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAcc                                                   <  2:698438/101‑1 (MQ=255)
                                              gcgcGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAcc                                                   <  1:5906277/101‑1 (MQ=255)
                                                gcgATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgc                                                 >  2:6476639/1‑101 (MQ=255)
                                                 cgATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgcc                                                <  2:6355680/101‑1 (MQ=255)
                                                  gATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgccg                                               >  2:3050991/1‑101 (MQ=255)
                                                   aTCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGACCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACcgccgc                                              >  1:6464902/1‑101 (MQ=255)
                                                    tCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCgccgcc                                             >  1:8248006/1‑101 (MQ=255)
                                                    tCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCgccgcc                                             >  2:5926092/1‑101 (MQ=255)
                                                      agcagcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGcccc                                           <  2:3904251/101‑1 (MQ=255)
                                                      agcagcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGcccc                                           >  1:4468420/1‑101 (MQ=255)
                                                       gcagcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCg                                          >  1:8626045/1‑101 (MQ=255)
                                                       gcagcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCg                                          <  1:3156318/101‑1 (MQ=255)
                                                        cagcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCgg                                         >  1:1685882/1‑101 (MQ=255)
                                                          gcgcCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGcc                                       <  2:5152447/101‑1 (MQ=255)
                                                          gccccGGCCGGGGCGCCAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCTCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGcc                                       <  2:8905269/98‑1 (MQ=255)
                                                             ccGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCa                                    >  1:4097111/1‑101 (MQ=255)
                                                               ggACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAg                                  <  1:6895628/101‑1 (MQ=255)
                                                                 aCGGGGGGCAAGCCCGCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAgcg                                >  1:8846375/1‑101 (MQ=255)
                                                                  cGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAgcgc                               >  1:5321346/1‑101 (MQ=255)
                                                                   ggggggCACGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGcc                              <  2:8157563/101‑1 (MQ=255)
                                                                   ggggggCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGcc                              <  1:9017793/101‑1 (MQ=255)
                                                                   gggggcCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGcc                              <  1:8205787/101‑1 (MQ=255)
                                                                    gggggCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCa                             <  1:8719349/101‑1 (MQ=255)
                                                                    gggggCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCa                             <  1:6590968/101‑1 (MQ=255)
                                                                     ggggCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAg                            <  1:4281426/101‑1 (MQ=255)
                                                                      gaccAAGCCCCCACCCCGAAAAGGCCACAAGCGCGAGCGCCAGCACCGCCGCGCGGAACGCCCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGc                           <  2:6806007/98‑1 (MQ=255)
                                                                        gCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCAc                         <  1:450870/101‑1 (MQ=255)
                                                                        gCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCAc                         >  2:529180/1‑101 (MQ=255)
                                                                         cAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCAcc                        <  2:1688899/101‑1 (MQ=255)
                                                                          aaGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCg                       >  1:8139669/1‑101 (MQ=255)
                                                                           aGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCgg                      <  2:3464471/101‑1 (MQ=255)
                                                                           aGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCgg                      >  2:8404771/1‑101 (MQ=255)
                                                                            gCCCCCCGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGgc                     <  1:2854024/101‑1 (MQ=255)
                                                                            gCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGgc                     <  2:4005235/101‑1 (MQ=255)
                                                                                ccAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGgcagca                 <  2:4109048/101‑1 (MQ=255)
                                                                                  aGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCAcc               <  2:6225297/101‑1 (MQ=255)
                                                                                   gCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCAccc              >  1:6082914/1‑101 (MQ=255)
                                                                                   gCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCAccc              <  1:2994994/101‑1 (MQ=255)
                                                                                   gCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCAccc              <  1:6351545/101‑1 (MQ=255)
                                                                                    cccGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCa             >  2:1529634/1‑101 (MQ=255)
                                                                                    cccGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCa             <  2:8595484/101‑1 (MQ=255)
                                                                                      cGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGc           <  2:6092589/101‑1 (MQ=255)
                                                                                      cGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGc           <  2:5547877/101‑1 (MQ=255)
                                                                                       gCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGcc          >  2:6029168/1‑101 (MQ=255)
                                                                                       gCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGcc          >  2:8699487/1‑101 (MQ=255)
                                                                                       gCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGcc          >  2:1774373/1‑101 (MQ=255)
                                                                                       gCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGcc          <  1:1023338/101‑1 (MQ=255)
                                                                                        cAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCg         <  2:2747505/101‑1 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCgg        >  1:5172437/1‑101 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCgg        >  2:3780293/1‑101 (MQ=255)
                                                                                           aGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGggc      >  1:6227790/1‑101 (MQ=255)
                                                                                           aGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGggc      >  1:1106220/1‑101 (MQ=255)
                                                                                               cACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGgcggcg  >  2:7718016/1‑101 (MQ=255)
                                                                                               cACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGgcggcg  <  2:3685194/101‑1 (MQ=255)
                                                                                               cACAAGCGCGAGCGCCAGCATCGCAGCGCGGAACGCGCCCCGGGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGgcggcg  >  2:4444991/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
AGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACGAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCG  >  NC_014034/2531477‑2531672

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG