Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,531,713 G→C 100% D496E (GAC→GAG RCAP_rcc02362 ← competence protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,531,7130G→C100.0% 1122.6 302D496E (GAC→GAGRCAP_rcc02362competence protein
Reads supporting (aligned to +/- strand):  new base (177/125):  ref base (0/0):  total (177/125)

Only 100 of 325 total aligned reads displayed.

GACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAAGTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCGG  >  NC_014034/2531615‑2531810
                                                                                                  |                                                                                                 
gcctgccccgccgCCCCGGCCGCAAGCGGCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGTTCTGCGCCCCCGCCAGACCCGCGACGAACTc                                                                                                 <  2:5710637/94‑1 (MQ=255)
gACGGCCCCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTc                                                                                                 <  2:841335/101‑1 (MQ=255)
gACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTc                                                                                                 >  1:740576/1‑101 (MQ=255)
     cACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggcca                                                                                            <  2:8674338/101‑1 (MQ=255)
      cccgccgCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccac                                                                                           <  1:4534515/100‑1 (MQ=255)
      aCCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccac                                                                                           >  1:1031384/1‑101 (MQ=255)
          ccgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccacggcc                                                                                       <  2:8605963/101‑1 (MQ=255)
          ccgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccacggcc                                                                                       >  1:4128701/1‑101 (MQ=255)
           cgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcgcccacggcaa                                                                                      >  2:7352724/1‑99 (MQ=255)
            gccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAg                                                                                     >  2:6320944/1‑101 (MQ=255)
             ccccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGc                                                                                    <  2:4468187/101‑1 (MQ=255)
             ccccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGc                                                                                    >  1:5647033/1‑101 (MQ=255)
              cccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCa                                                                                   <  1:6772793/101‑1 (MQ=255)
               ccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAc                                                                                  >  1:2384008/1‑101 (MQ=255)
                cGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCAGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAcc                                                                                 >  1:8182946/1‑101 (MQ=255)
                 ggCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAccc                                                                                >  1:2223641/1‑101 (MQ=255)
                 ggCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAccc                                                                                >  2:2583300/1‑101 (MQ=255)
                   ccGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAg                                                                              <  2:3314312/101‑1 (MQ=255)
                    cGCAGGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGt                                                                             <  2:8045581/101‑1 (MQ=255)
                      cAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCg                                                                           >  2:158494/1‑101 (MQ=255)
                      cAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCg                                                                           >  1:1181569/1‑101 (MQ=255)
                          tccgatctccGGCAGCCCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGc                                                                       <  1:2021535/93‑1 (MQ=255)
                          cgcCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGc                                                                       <  2:7768095/101‑1 (MQ=255)
                            ccAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGccc                                                                     >  2:7253305/1‑101 (MQ=255)
                            ccAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGccc                                                                     >  1:6367438/1‑101 (MQ=255)
                              aGCACCGGCAGCCCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAg                                                                   <  1:7053807/101‑1 (MQ=255)
                              aGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAg                                                                   >  2:1887943/1‑101 (MQ=255)
                               gCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGc                                                                  >  2:2816123/1‑101 (MQ=255)
                                  cgtgctgcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGcccc                                                               >  2:6554534/7‑101 (MQ=255)
                                  ccGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGcagatcgga                                                               >  2:2021474/1‑93 (MQ=255)
                                    ggCAGCACCCAGGCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCAt                                                             >  1:6501175/1‑101 (MQ=255)
                                    ggCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCAt                                                             <  2:7044315/101‑1 (MQ=255)
                                     gcagcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATc                                                            <  1:1648945/101‑1 (MQ=255)
                                     gcagcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATc                                                            <  1:1109147/101‑1 (MQ=255)
                                      cagcaCCCGGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCa                                                           <  1:1835112/101‑1 (MQ=255)
                                      cagcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCa                                                           >  1:2584998/1‑101 (MQ=255)
                                       agcaCCCAGCCGGGCGGGGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGCCGGTGCCCCGCCCCATCac                                                          >  1:9182159/1‑100 (MQ=255)
                                       agcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAg                                                          <  2:8182597/101‑1 (MQ=255)
                                       agcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAg                                                          >  1:7599087/1‑101 (MQ=255)
                                       agcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAg                                                          >  1:7267849/1‑101 (MQ=255)
                                       agcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAg                                                          <  1:3748720/101‑1 (MQ=255)
                                        gcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGc                                                         >  1:1450620/1‑101 (MQ=255)
                                         caCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGcc                                                        >  2:6008914/1‑101 (MQ=255)
                                          ccccAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCa                                                       <  2:8147651/100‑1 (MQ=255)
                                          aCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCa                                                       >  2:4923415/1‑101 (MQ=255)
                                           cccAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCag                                                      >  1:4619345/1‑101 (MQ=255)
                                              aGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgc                                                   >  1:2904010/1‑101 (MQ=255)
                                              aGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgc                                                   >  2:8451212/1‑101 (MQ=255)
                                                ccGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgcgc                                                 >  2:6819434/1‑101 (MQ=255)
                                                 cGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGcgcg                                                >  2:2079157/1‑101 (MQ=255)
                                                  gggCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCgg                                               <  2:6286400/101‑1 (MQ=255)
                                                   ggcggcGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCggg                                              >  1:5149732/1‑101 (MQ=255)
                                                   ggcggcGCGGGCAGAAAGGTCTGCCCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCggg                                              >  2:4381053/1‑101 (MQ=255)
                                                    gcggcgCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGc                                             <  1:6675851/101‑1 (MQ=255)
                                                      ggcgCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGccc                                           >  1:3258388/1‑101 (MQ=255)
                                                      ggcgCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGccc                                           >  1:4453645/1‑101 (MQ=255)
                                                      ggcgCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGccc                                           >  2:1201923/1‑101 (MQ=255)
                                                       gcgcGGGCAGAAAGGGCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCAAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGcccc                                          <  2:1100974/101‑1 (MQ=255)
                                                        cgcgGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCg                                         >  1:5033250/1‑101 (MQ=255)
                                                           gggCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCa                                      >  2:7169886/1‑101 (MQ=255)
                                                           gggCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCACCACCCAGTCGGTGCCCAGCCCCATCAGCCATAGCGCGGGCCCCACCa                                      >  2:8852166/1‑101 (MQ=255)
                                                            ggCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCCGCCCCATCAGCCAGAGCGCGGGCCCCGCCAg                                     >  2:4502258/1‑101 (MQ=255)
                                                               acaaaGGCCTGCCCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAcc                                  <  1:6199719/99‑1 (MQ=255)
                                                                gAACGGTCCGCGCCCCGGCCAGCCCCACGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAccc                                 <  2:2939346/101‑1 (MQ=255)
                                                                gAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAccc                                 <  1:1985225/101‑1 (MQ=255)
                                                                 aaaGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCa                                >  2:2688349/1‑101 (MQ=255)
                                                                   aGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgc                              >  2:5049859/1‑101 (MQ=255)
                                                                   aGGTCTGCCCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgc                              <  1:3058030/101‑1 (MQ=255)
                                                                    ggTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACGCAgcg                             <  2:6227551/101‑1 (MQ=255)
                                                                        tGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGgcgc                         >  1:3003422/1‑101 (MQ=255)
                                                                         gcgcCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGcc                        >  1:5922231/1‑101 (MQ=255)
                                                                          cgcCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCTGCCAGACCCAGCGGCGCCa                       <  1:5746517/101‑1 (MQ=255)
                                                                          cgcCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCa                       <  2:2223574/101‑1 (MQ=255)
                                                                           gcCCCCGCCAGCCCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAg                      <  2:3126425/101‑1 (MQ=255)
                                                                           gcCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGcccg                      >  2:3076954/1‑99 (MQ=255)
                                                                           gcCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAg                      >  2:1752074/1‑101 (MQ=255)
                                                                           gcCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAg                      >  1:8867614/1‑101 (MQ=255)
                                                                             ccccGCCAGACCCGCGACGAACTCGCCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGaa                    <  1:7353048/101‑1 (MQ=255)
                                                                                cGCCAGACCCGCGACGACCTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccg                 <  2:4128582/101‑1 (MQ=255)
                                                                                cGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccg                 >  2:4831170/1‑101 (MQ=255)
                                                                                 gCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCTGCTACACCCAGCGGCGCCAGAActgc                >  2:9035381/1‑101 (MQ=255)
                                                                                 gCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgc                >  2:2375707/1‑101 (MQ=255)
                                                                                  ccggACCCGCGACGCACTCGGCCACGGCCAGCGCCCCGGCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgcc               <  1:7789697/98‑1 (MQ=255)
                                                                                   cAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgccg              >  1:9077175/1‑101 (MQ=255)
                                                                                    aGACCCGCGACGACCTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACcgccgc             <  2:3523137/101‑1 (MQ=255)
                                                                                    aGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACcgccgc             >  1:2760939/1‑101 (MQ=255)
                                                                                     gACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCgccgcc            <  2:7598760/101‑1 (MQ=255)
                                                                                      aCCCGCGACGACCTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCa           <  2:3945016/101‑1 (MQ=255)
                                                                                      aCCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCa           <  2:5251836/101‑1 (MQ=255)
                                                                                         cgcgGCGAACTCGGCCCCGGCCAGCCCCCAGCCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCa        <  2:5113058/101‑1 (MQ=255)
                                                                                         cgcgACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCa        <  2:4578405/101‑1 (MQ=255)
                                                                                         cgcgACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCa        <  1:4875096/101‑1 (MQ=255)
                                                                                          gcgACGAACTCGGCCACGGCCGGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCAc       <  2:5492232/101‑1 (MQ=255)
                                                                                          gcgACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCAc       >  1:8613060/1‑101 (MQ=255)
                                                                                          gcgACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCAc       >  1:3603910/1‑101 (MQ=255)
                                                                                            gacgaACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCAccc     >  1:8093399/1‑101 (MQ=255)
                                                                                             acgaACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCAcccc    <  1:3459711/101‑1 (MQ=255)
                                                                                              cgaACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCg   >  1:5382729/1‑101 (MQ=255)
                                                                                               gaACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCgg  >  2:1413947/1‑101 (MQ=255)
                                                                                               gaACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCgg  >  1:2163903/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAAGTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCGG  >  NC_014034/2531615‑2531810

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG