Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,549,153 G→A 100% G56S (GGC→AGC)  tonB → protein TonB

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,549,1530G→A100.0% 1338.7 351G56S (GGC→AGC) tonBprotein TonB
Reads supporting (aligned to +/- strand):  new base (158/190):  ref base (2/1):  total (160/191)

Only 100 of 374 total aligned reads displayed.

CGCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGAC  >  NC_014034/2549055‑2549251
                                                                                                  |                                                                                                  
cGCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGc                                                                                                  >  1:6206312/1‑101 (MQ=255)
 gCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGcc                                                                                                 >  1:5867291/1‑101 (MQ=255)
   tGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTc                                                                                               <  1:6593749/101‑1 (MQ=255)
    ggCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACCGCCTCg                                                                                              >  2:3845208/1‑101 (MQ=255)
    ggCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCg                                                                                              <  1:7567403/101‑1 (MQ=255)
    ggCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCg                                                                                              <  1:24185/101‑1 (MQ=255)
     gcgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGa                                                                                             <  1:5146258/101‑1 (MQ=255)
     gcgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGa                                                                                             <  1:1968972/101‑1 (MQ=255)
     ccgatctaTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGa                                                                                             <  2:7995537/94‑1 (MQ=255)
      cgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGGTCCGGACAGCCTccca                                                                                            >  2:8356166/1‑98 (MQ=255)
      cgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGaa                                                                                            <  2:3550347/101‑1 (MQ=255)
      cgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGaa                                                                                            <  1:3703092/101‑1 (MQ=255)
      cgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTCGCGGCGGGCGCGGGCGGCGAGGACCTGGACAGCCTCGaa                                                                                            >  2:9000933/1‑101 (MQ=255)
       gcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAg                                                                                           <  2:1491145/101‑1 (MQ=255)
       gcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAg                                                                                           <  2:2009198/101‑1 (MQ=255)
       gcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAg                                                                                           <  2:7141441/101‑1 (MQ=255)
        cTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGc                                                                                          <  1:1215272/101‑1 (MQ=255)
         tGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCg                                                                                         <  1:1467300/101‑1 (MQ=255)
         tGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCg                                                                                         >  1:1392000/1‑101 (MQ=255)
         tGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCg                                                                                         >  2:512581/1‑101 (MQ=255)
          gCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGCCCGCCCCCGCGGCGCTGGTGGCGGCGGCCGCGGGCGGCTCGGCGCTTGACAGCGGCGAAGCgg                                                                                        >  2:9123289/1‑100 (MQ=255)
             tGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcg                                                                                     <  1:9028771/101‑1 (MQ=255)
                  gcgcgGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCcgcg                                                                                <  2:7694944/100‑1 (MQ=255)
                   cgcgGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGGCAGCCTCGAAGCGATCGCCGCGa                                                                               >  1:468172/1‑101 (MQ=255)
                   cgcgGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCcgcgn                                                                               >  1:4818756/1‑100 (MQ=255)
                   cgcgGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGa                                                                               >  1:6890555/1‑101 (MQ=255)
                        tttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtc                                                                          <  2:6206073/101‑1 (MQ=255)
                        tttCCGTGCCCTCGCATCCCGATCCCTCCGCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtc                                                                          <  2:4231056/101‑1 (MQ=255)
                         ttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGGCAGCCTCGAAGCGAGCGCCGCGACCTtcg                                                                         >  2:797327/1‑101 (MQ=255)
                          tCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgt                                                                        <  2:5830860/101‑1 (MQ=255)
                           ccttGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGGAGCGATCGCCGCGACCTtcgtc                                                                       >  2:5348622/4‑101 (MQ=255)
                           ccGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtc                                                                       >  1:7119721/1‑101 (MQ=255)
                           ccGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtc                                                                       <  1:6774159/101‑1 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                      >  1:6058649/1‑101 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                      <  1:2945349/101‑1 (MQ=255)
                             gTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGa                                                                     >  1:5623477/1‑101 (MQ=255)
                              tGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGAt                                                                    <  1:6340184/101‑1 (MQ=255)
                              tGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGAt                                                                    <  1:2196333/101‑1 (MQ=255)
                               gCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATc                                                                   <  1:5032386/101‑1 (MQ=255)
                                 tctcGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTg                                                                 <  2:4348894/101‑1 (MQ=255)
                                  ctcGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGCTCTgg                                                                >  2:5011762/1‑101 (MQ=255)
                                  ctcGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTgg                                                                >  1:2793717/1‑101 (MQ=255)
                                  ctcGCAGACCGAAGCCGCCCCCGCGGCGGTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTgg                                                                >  1:8081756/1‑101 (MQ=255)
                                   tcGCAGACCGAGTCCGTACCCACCTCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGTTCTTGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGgc                                                               <  1:9123711/101‑1 (MQ=255)
                                   tcGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGgc                                                               <  1:420828/101‑1 (MQ=255)
                                   tcGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGgc                                                               <  1:3281214/101‑1 (MQ=255)
                                      cAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgcc                                                            <  2:6996298/101‑1 (MQ=255)
                                       aGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccg                                                           >  2:1865834/1‑101 (MQ=255)
                                       aGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccg                                                           <  1:3048900/101‑1 (MQ=255)
                                          ccGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAg                                                        <  1:2298106/101‑1 (MQ=255)
                                           cGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGa                                                       >  1:8600650/1‑101 (MQ=255)
                                            gAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGAt                                                      >  2:1215379/1‑101 (MQ=255)
                                             aGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATc                                                     <  2:5391214/101‑1 (MQ=255)
                                              ggCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATcc                                                    <  2:8561223/101‑1 (MQ=255)
                                              ggCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATcc                                                    <  2:5560157/101‑1 (MQ=255)
                                               gccgccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCa                                                   >  1:1769107/1‑101 (MQ=255)
                                                     cccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGAACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcc                                             >  2:4450427/1‑101 (MQ=255)
                                                      ccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgccc                                            <  1:3225043/101‑1 (MQ=255)
                                                      ccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgccc                                            <  2:7224730/101‑1 (MQ=255)
                                                       cgcgGCGCTGGTGGCGGCGGGCGCGGGCTGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccg                                           <  2:3406546/101‑1 (MQ=255)
                                                       cgcgGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccg                                           <  2:21803/101‑1 (MQ=255)
                                                       cgcgGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccg                                           <  1:4121111/101‑1 (MQ=255)
                                                       cgcgGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccg                                           <  1:3906368/101‑1 (MQ=255)
                                                        gcggcgCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccga                                          >  1:162565/1‑101 (MQ=255)
                                                          ggcgCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagc                                        <  1:643098/101‑1 (MQ=255)
                                                            cgcTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagccc                                      <  1:8473602/101‑1 (MQ=255)
                                                             gcTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcccg                                     <  2:1381648/101‑1 (MQ=255)
                                                               tggtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGcccgagcccgag                                   <  2:7491473/101‑1 (MQ=255)
                                                                ggtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCgaga                                  >  2:3152579/1‑101 (MQ=255)
                                                                 gtggCGGCGGGCGCGGGCGGCGAGGGTCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCgagag                                 >  2:4916933/1‑100 (MQ=255)
                                                                 gtggCGGCGGGCGCGGGCGACGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGGTCCAGCTGCCCGAGCCCGAGAt                                 >  1:5512582/1‑101 (MQ=255)
                                                                  tggCGGCGGGCGCGGGCGGCGAGGGTCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATg                                <  2:4653712/101‑1 (MQ=255)
                                                                      ggcgggcgCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGgcgc                            >  2:8123236/1‑101 (MQ=255)
                                                                        cgggcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTg                          <  2:4000614/101‑1 (MQ=255)
                                                                         gggcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCTATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGc                         <  1:8197518/101‑1 (MQ=255)
                                                                           gcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCg                       <  1:7936331/101‑1 (MQ=255)
                                                                            ggcgggtggAGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTGGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGc                      <  1:7599456/100‑1 (MQ=255)
                                                                             gcgggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCt                     <  1:7736565/101‑1 (MQ=255)
                                                                               gggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGa                   >  2:1650811/1‑101 (MQ=255)
                                                                                ggcggcGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAc                  <  1:8930599/101‑1 (MQ=255)
                                                                                ggcggcGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAc                  >  1:7360609/1‑101 (MQ=255)
                                                                                  cggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcg                >  2:8149377/1‑101 (MQ=255)
                                                                                  cggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcg                <  1:3481563/101‑1 (MQ=255)
                                                                                   ggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcgc               <  2:6346715/101‑1 (MQ=255)
                                                                                   ggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcgc               >  2:1307202/1‑101 (MQ=255)
                                                                                   ggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcgc               >  2:2369310/1‑101 (MQ=255)
                                                                                    gcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcgcg              >  1:5841466/1‑101 (MQ=255)
                                                                                     cgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCgcgc             >  2:6064506/1‑101 (MQ=255)
                                                                                      gAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGcc            >  1:1165908/1‑101 (MQ=255)
                                                                                       aGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCg           <  2:6420510/101‑1 (MQ=255)
                                                                                        ggATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCgg          >  1:8796357/1‑101 (MQ=255)
                                                                                         gATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGt         <  1:4365201/101‑1 (MQ=255)
                                                                                          aTCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTg        >  1:5288114/1‑101 (MQ=255)
                                                                                            cTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGAt      >  1:7508095/1‑101 (MQ=255)
                                                                                            cTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGGGAt      >  2:7304393/1‑101 (MQ=255)
                                                                                             tGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATc     >  1:8009539/1‑101 (MQ=255)
                                                                                              ggACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCg    <  2:7869105/101‑1 (MQ=255)
                                                                                              ggACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCg    >  1:1704247/1‑101 (MQ=255)
                                                                                               gACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGa   <  2:6562302/101‑1 (MQ=255)
                                                                                                aCGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGAc  <  1:2029240/101‑1 (MQ=255)
                                                                                                  |                                                                                                  
CGCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGAC  >  NC_014034/2549055‑2549251

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG